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Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis

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Journal of Ocean University of China 2019年第6期18卷 1451-1469页

作者： MU Danli DU Chen FU Suyan wang jingqian HOU Congcong TANG Daojun ZHU Junquan The Key Laboratory of Applied Marine Biotechnology of Ministry of Education School of Marine SciencesNingbo University

KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in *** understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea,we studied the testis characteristics at different developmental stages of ***,and determined the spatiotemporal expression patterns of kif3a and kif3b during *** real-time PCR(qR T-PCR)showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are *** stage Ⅱ to stage V,kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage ***,the m RNA abundances of both genes at stage V were higher than those at stages Ⅱ and Ⅲ.In addition,it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all *** in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria,the deformation of nucleus,and the formation of *** m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei,then appeared at one side of nuclei,and finally were mainly on the tail during *** findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish;and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria,nuclear deformation,and the formation of tail during the spermiogenesis in ***.

关键词： Larimichthys crocea kif3a/3b spermiogenesis expression pattern

Evidence for the role of KIF17 in fish spermatid reshaping:expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

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Journal of Oceanology and Limnology 2021年第6期39卷 2322-2335页

作者： jingqian wang Xinming GAO Xuebin ZHENG Chen DU Congcong HOU Qingping XIE Bao LOU Feng LIU Shan JIN Junquan ZHU Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Zhejiang Academy of Agricultural Sciences Hangzhou 310021China

The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in ***,its role in fish spermiogenesis has not been ***,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in Larimichthys polyactis *** full-length cDNA of *** kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino *** analyses showed that *** KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail *** Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the *** in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this *** immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during *** findings suggested that KIF17 is involved in *** *** particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid *** addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in ***.

关键词： KIF17 spermiogenesis Larimichthys polyactis spermatid reshaping

Testis Development and Ultrastructural Features During Spermatogenesis in Cultured Small Yellow Croaker,Larimichthys polyactis

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Journal of Ocean University of China 2023年第5期22卷 1412-1426页

作者： ZHENG Xuebin wang jingqian GAO Xinming DU Chen HOU Congcong XIE Qingping LIU Feng ZHU Junquan LOU Bao Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China Zhejiang Academy of Agricultural Sciences Hangzhou 310021China

The small yellow croaker Larimichthys polyactis is an economically important marine fish in Northeast ***,its natural resources are threatened by overfishing and environmental ***,research on the reproductive system of the fish is ***,we studied the testis development and ultrastructural features of spermatogenesis in cultured *** using anatomical,histological,and ultrastructural techniques.A pair of testes,consisting of a central sperm duct and radial seminiferous lobules,were *** reproduction cycle of testes can be divided into stages I–*** to May was confirmed as the breeding season for male ***,while April is the ideal period for artificial *** male *** can attain sexual maturity within 1 *** spermatogenesis of *** comprised spermatogonium,spermatocyte,spermatid,and mature *** morphology of spermatogenic cells changed obviously during spermiogenesis,including nuclear shaping,midpiece and flagellum *** mature sperms consist of an ellipsoidal head,a short midpiece,and a long *** anterior of the head with a kidney-shaped nucleus can be *** midpiece is located posterior to the head and includes four to six spherical *** flagellum has irregular lateral *** testis of *** is an unrestricted lobular type,with cystic spermatogenesis,type II spermiogenesis,and type II *** features are highly similar to those of other Sciaenid *** findings provide useful insights into the mechanism underlying testis development and spermatogenesis of ***,which can facilitate the artificial breeding of this species.

关键词： testis development spermatogenesis Larimichthys polyactis histology ultrastructure

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基于网格的语音关键词检索算法改进

清华大学学报（自然科学版） 2015年第5期55卷 508-513页

作者：肖熙王竞千清华大学电子工程系北京100084

针对多候选汉语音节网格语音关键词检索任务,在Gauss混合模型以及多候选识别算法方面进行了研究改进。首先探讨了Gauss混合模型的不同简化策略并用实验进行了验证,证明了全协方差矩阵在识别性能上的优越性;随后对经典的多候选令牌传递... 详细信息

针对多候选汉语音节网格语音关键词检索任务,在Gauss混合模型以及多候选识别算法方面进行了研究改进。首先探讨了Gauss混合模型的不同简化策略并用实验进行了验证,证明了全协方差矩阵在识别性能上的优越性;随后对经典的多候选令牌传递算法做出了针对汉语特点的改进。实验表明这2方面的研究不仅提高了以音节作为输出的语音识别引擎的单候选识别效果,也大幅提高了多候选的识别性能。最后搭建了一个基于多候选网格的语音关键词检索系统,在该系统中验证了上述改进的效果。

关键词：语音关键词检索多候选网格 Gauss混合模型 CUDA 三音子模型

Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways

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Journal of Oceanology and Limnology 2023年第6期41卷 2429-2443页

作者： Shengyu LUO Cheng LIU Xinming GAO jingqian wang Yibo ZHANG Jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Zhejiang Academy of Agricultural SciencesHangzhou 310021China State Key Laboratory of Large Yellow Croaker Breeding Ningbo Academy of Oceanology and FisheryNingbo 315012China

Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in *** is a consequence of hypoxia on ***,the effects of hypoxia stress on apoptosis in *** remain largely *** investigated the effect of environmental hypoxia on apoptosis in *** show that hypoxia induced apoptosis in *** both in vivo and in *** mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)*** expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in *** expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF *** level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of ***,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic *** addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in ***,hypoxia induced apoptosis in *** through both the intrinsic and extrinsic *** present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

关键词： hypoxia large yellow croaker mitochondrial apoptosis pathway death receptor apoptosis pathway

西藏农村社会工作发展的经验及启示——以墨脱县社会工作站建设为例

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民族研究 2023年第5期 30-44,M0003页

作者：王景迁鲁东大学法学院烟台市264025

2023年中央一号文件明确要求深化农村社会工作服务。西藏是边疆民族地区,属于社会工作“后发”省区,农村社会工作的发展会受到多重因素的影响,既有普遍性,又有特殊性。墨脱县立足自身实际情况,创新农村社会发展模式,在西藏最早引入社会... 详细信息

2023年中央一号文件明确要求深化农村社会工作服务。西藏是边疆民族地区,属于社会工作“后发”省区,农村社会工作的发展会受到多重因素的影响,既有普遍性,又有特殊性。墨脱县立足自身实际情况,创新农村社会发展模式,在西藏最早引入社会工作参与农村社会服务和社会治理,走出了一条特色鲜明的“内源与外援”相结合的社会工作发展之路,积累了一定经验,成为西藏农村社会工作发展先行县。本文总结了墨脱县农村社会工作发展的举措和基本经验,希望能够对西藏其他县市区农村社会工作发展有所借鉴。

关键词：西藏农村社会工作社会服务基层治理

“百年中共思想史研究的学术展望”座谈会综述

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中共党史研究 2021年第5期 153-158页

作者：王婧倩中共中央党史和文献研究院

作为中共党史研究的一个传统论域,思想史研究在梳理中国共产党的思想发展脉络和结构以及整合具体党史问题研究和推动学术创新等诸多方面,发挥着不可替代的积极作用,具有非常显著的学术地位。20世纪80年代以来,中共思想史研究历经多个发... 详细信息

作为中共党史研究的一个传统论域,思想史研究在梳理中国共产党的思想发展脉络和结构以及整合具体党史问题研究和推动学术创新等诸多方面,发挥着不可替代的积极作用,具有非常显著的学术地位。20世纪80年代以来,中共思想史研究历经多个发展阶段,进入新世纪后取得了新的学术进展。在纪念中共成立一百周年的特殊语境下,为继续推动中共思想史研究及其理论、方法和体系建设,由《中共党史研究》编辑部和杭州师范大学人文学院联合主办的“百年中共思想史研究的学术展望”学术座谈会于5月29日至30日在杭州举行,来自中共中央党史和文献研究院、中国社会科学院、北京大学、清华大学、中国人民大学、北京师范大学、中国政法大学、复旦大学、浙江大学、中山大学、厦门大学、山东大学、四川大学、华东师范大学、华南师范大学、上海大学、上海师范大学、山东师范大学、国防大学、河南大学、湖南工商大学、中共福建省委党校和杭州师范大学等单位的50余名专家学者与会。

关键词：山东师范大学华南师范大学中国政法大学上海大学人文学院中国人民大学中共福建省委学术创新

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失独家庭社会救助现状与对策研究

北京大学学报（哲学社会科学版） 2019年第5期56卷 77-86页

作者：王景迁方卫鲁东大学法学院

失独问题是典型的计划生育政策后遗症问题,有其自身特性;做好失独救助工作,必须要对其特性有充足认识,这需要在理论层面与实践层面做出调整。解决失独人士的生活、医疗、养老与心理问题,是失独救助工作的核心。Y市是我国开展计划生育工... 详细信息

失独问题是典型的计划生育政策后遗症问题,有其自身特性;做好失独救助工作,必须要对其特性有充足认识,这需要在理论层面与实践层面做出调整。解决失独人士的生活、医疗、养老与心理问题,是失独救助工作的核心。Y市是我国开展计划生育工作最早的地区之一,失独问题突出,是计划生育特殊家庭典型化地区。通过对Y市失独救助工作的回顾与探讨,认为解决当前的失独问题要从宏观、中观与微观三个层面进行解决,涉及政策设计、社区资源的整合、社会工作专业方法的介入等问题。做好救助工作的同时,还要提防群体风险演变为社会风险的可能,尤其警惕其政治化倾向。

关键词：失独家庭社会救助社工介入心理救助幸福感

Petrogenesis of Adakitic Intrusive Rocksin Xu-Huai Area, Eastern China

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Global Geology 2003年第2期8卷 141-148页

作者： Qinghai wang, Wenliang Xu, Dongyan wang, jingqian LinCollege of Earth Sciences, Jilin University, Changchun, 130061 China College of Earth Sciences Jilin University Changchun 130061 China

The research of the petrology and the geochemistry of the Mosozoic intrusive rocks in Xu-Huai area indicated that they are mainly composition of dioritic porphyry and monzodioritic porphyry, and the features of the petrology and geochemistry of the Mosozoic intrusive rocks are extremely similar to those with the typical adakites. Combined with the petrography and the testimated results of the deep-seated xenoliths in the intrusive rocks, it indicates that the petrogenesis of the Mosozoic intrusive rocks could be resulted from the partial melting of amphibole-bearing garnet pyroxenite and garnet amphibolite in lower continental crust in this area.

关键词： adakite, petrogenesis, intrusive rocks, partial melting, Xu-Huai area