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Cloning of the key genes in maize oxylipins pathways and their roles in herbivore induced defense

Chinese Science Bulletin 2005年第21期50卷 2457-2466页

作者： XU Tao wang jianwu LUO Shiming Institute of Tropical and Subtropical Ecology South China Agricultural University Guangzhou 510642 China

To gain an understanding of the molecular ba-sis of signaling pathways in herbivore-induced maize plant defense, three key genes, ZmAOS, ZmAOC and ZmHPL, which are involved in the biosynthesis of oxylipin signals, have been cloned using RT-PCR in this study. Beet army-worm (BAW) infestation induced the systemic expression of the key genes involved in the biosynthesis of oxylipin signals similar to exogenous methyl jasmonate (MeJA). Moreover, the systemic expression patterns of maize defense-related genes were similar between maize leaves induced by jas-monic acid (JA) and damaged by BAW. Previous treatment with salicyhydroxamic acid (SHAM), an inhibitor of jas-monates (JAs) signal pathway followed by BAW infestation did not induce the systemic expression of the defense-related genes. Exposure to the vapors of green leafy volatiles (GLVs, (Z)-3-hexen-1-ol, (E)-2-hexenal, (E)-3-hexenal) and β-oci- mene induced the expression of the defense-related genes, as well as the key genes involved in biosynthesis of JAs. How-ever, previous treatment with SHAM clearly decreased the transcript levels of the defense genes induced by (Z)-3-hexen-1-ol, (E)-2-hexenal and (E)-3- hexenal. These results demonstrate the major role of oxylipin signal path-way in herbivore-induced maize chemical defense. JA was the endogenous signal in the process of herbivore-induced maize systemic defense. GLVs, another group of oxylipin, played an important role in the process of herbivore-induced systemic defense outside the plant. Furthermore, the expres-sion of defense-related genes induced by GLVs was partially dependent on JAs signal pathway, while β-ocimene induction was independent of JAs signal pathway.

关键词：玉米繁殖技术基因表达茉莉酮

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Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

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Animal Nutrition 2018年第3期4卷 300-304页

作者： Shuiyue Zhou Yuanxin Hang jianwu wang Rejun Fang College of Animal Science and Technology Hunan Agricultural University Xiangya School of Public Health Central South University

This study was to identify the effects of prebiotics supplemented in infant formula on enzyme activity and phosphate uptake in the small intestine of Sprague Dawley(SD) rats. Forty-eight healthy SD rats at15 days old(a week before weaning) with similar weight were randomly divided into 3 groups: A(control group), B, C, with 16 rats per group. Rats in groups A, B, C were fed a standard infant formula, the standard infant formula supplemented with oligosaccharides, and the standard infant formula supplemented with polysaccharides, respectively. The feeding test was conducted for 28 d. Compared with group A, the results showed the following: 1) the activities of sucrose and lactase in the small intestine were significantly increased in SD rats of group C(P < 0.05); 2) the relative expressions of lactase gene in the anterior and posterior segments of the small intestine were significantly increased by 1.68 and 2.26 in SD rats of group C(P < 0.05), and the relative expression of Mgam gene in the posterior segment of the small intestine was significantly increased by 0.99 in SD rats of group C(P < 0.05); 3) the relative expressions of Na/Pi-IIb gene in the anterior and posterior segments of the small intestine were significantly increased by 1.85 and 2.28 in SD rats of group C(P < 0.05). These results indicate that the supplementation of infant formula with prebiotics can promote enzyme activity in the small intestine by increasing the relative expression of enzyme gene or by decreasing intestinal injury, and can increase the relative expression of Na/Pi-IIb gene. The effect of polysaccharides is better than that of oligosaccharides.

关键词： Infant formula Prebiotics Ussing chamber Intestinal enzyme activities Phosphate absorption

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Conjugated polymer nanoparticles as fluorescence switch for selective cell imaging

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Chinese Chemical Letters 2020年第3期31卷 755-758页

作者： Ke Peng Fengting Lv Huan Lu jianwu wang Hao Zhao Libing Liu Shu wang Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic SolidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China College of Chemistry University of Chinese Academy of SciencesBeijing 100049China

Fluorescence switch plays a vital role in bioelectronics and ***,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual *** ECNs were prepared by mixing positively charged poly(6-(2-(thiophen-3-yl)ethoxy)hexyl trimethylammonium bromide)(PT)and negatively charged diarylethene sodium salt(DAECOONa).DAE-COONa is a photoswitchable molecule which can be transformed between the ring-closed fo rm and ring-open form under the irradiation of UV or visible *** closed-form of DAE-COONa can efficie ntly quench the fluorescence of PT through intermolecular energy transfer,while the open form of DAE-COONa does not influence the emission of ***,the fluorescence of ECNs can be modulated by light irradiation,and the ECNs with good fluorescence switching performance have been employed for fluorescence imaging and individual cell lighting up process *** demonstrate that the electrostatic complex strategy provides a facile method to construct fluorescence switch fo r selective cell marking and imaging applications.

关键词： Electrostatic complex nanoparticles Conjugated polymers Fluorescence switch Cell imaging Selective cell lighting up

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Facile Synthesis of Enantiomerically Pure 1-（5-Bromo-2-chlorophenyl）-1-（4-ethoxyphenyl）ethane

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Chemical Research in Chinese Universities 2014年第2期30卷 250-256页

作者： ZHANG Shuo wang Wenjin LI Chuan LIU Peng XU Weiren TANG Lida wang jianwu ZHAO Guilong School of Chemistry and Chemical Engineering Shandong University Jinan 250100 P.R.China Tianjin Key Laboratory of Molecular Design and Drug Discovery Tianjin Institute of Pharmaceutical ResearchTianjin 300193 P.R.China

A facile 7-step procedure for the synthesis of enantiomerically pure 1-（5-bromo-2-chlorophenyl）-1-（4-ethoxyphenyl）ethanes[（R）-2 and（S）-2] that started from （5-bromo-2-chlorophenyl）（4-ethoxyphenyl）methanone 3 was *** key step was the resolution of 2-（5-bromo-2-chlorophenyl）-2-（4-ethoxyphenyl）acetic acid 6 by crystallizations of its L-and D-menthyl esters 7 and 8 from petroleum ether to give optically pure enantiomers 9 and 10,*** absolute configurations of the products were unambiguously determined by single-crystal X-ray diffractions of four key intermediates,9,10,13 and *** procedure is characterized by inexpensiveness,scalability and ability to produce two individual enantiomers of a diarylethane with unambiguously determined absolute configurations and high enantiomeric purities.

关键词： Diarylethane Diastereomer Enantiomer Resolution SGLT2 inhibitor

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Climate capacity:the measurement for adaptation to climate change

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Chinese Journal of Population,Resources and Environment 2015年第2期13卷 99-108页

作者： Jiahua Pan Yan Zheng jianwu wang Xinlu Xie Institute for Urban and Environmental Studies Chinese Academy of Social Sciences Research Center for Sustainable Development Chinese Academy of Social Sciences Legal Centre for Land and Mineral Resources The Ministry of Land and Resources

This article proposed the concept of"climate capacity"as a way of measuring human's adaptiveness to climate *** article also focused on the related concepts like ecological carrying capacity,water resources carrying capacity,land carrying capacity as well as population carrying *** concept of climate capacity was articulated against a background of global climate and environmental ***,China's efforts to adapt to climate change was a matter of improving climate capacity,which is the ecosystem as well as the frequency,the intensity and the scale of human's social activities that the climatic resources of a particular geographic area were supposed to *** climate capacity has two *** is the natural climate capacity,which includes temperature,sunlight,precipitation,extreme climatic events,*** other is the derived climate capacity,which includes water resources,land resources,ecological systems,climatic risks,*** climate capacity can be developed or be transferred between regions by taking engineering,technology or regime-based adaptive ***,these adaptive measures must be implemented under the principle of economic rationalism,ecological integrity,climate protection,and social *** is expected that by combining the climate capacity and its threshold value with the assessment of climate change risks,we are able to predict the optimal population carrying capacity and the scale of socioeconomic development,and furthermore,provide policy support for the socioeconomic development strategy and adaptive *** the regions with high climate capacity,there is a symbiotic relationship between adaptation and socioeconomic ***,in the regions with limited climate capacity,irrational development may further damage the *** the Yangtze River delta,a region with high climate capacity,and a region of Ningxia,a region with limited climate capacity,as illustrative examples,the author

关键词： climate change climate capacity adaptation development climate change economics

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A Facile Synthetic Approach to 3-Halo-4-substituted-l,5-dihydro-4H-1,2,4-triazoline-5-thiones Based on Retro-Michael Addition

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Chemical Research in Chinese Universities 2017年第4期33卷 587-593页

作者： TIAN He LIU Yuqiang LI Xingwei XIE Yafei LIU Changying wang jianwu ZHAO Guilong School of Chemistry and Chemical Engineering Shandong University Jinan 250100 P. R. China Eanjin Key Laboratory of Molecular Design and Drug Discovery Eanjin Institute of Pharmaceutical Research Tianjin 300193 P. R. China

A facile 3-step synthetic approach to 3-halo-4-substituted-l,5-dihydro-4H-1,2,4-triazolme-5-thiones （la--lk） from corresponding unhalogenated 8a---8i was developed based on the strategy of retro-Michael addition, with the key steps being regioselective S-alkylation of compounds 8a-8i with ethyl 3-iodopropionate（12c） and alkaline cleavage of the propionate protecting group via retro-Michael addition. The synthetic approach was characterized by simplicity of operation and wide substrate scope.

关键词： Synthetic approach Protecting group Triazole Halogenation Regioselectivity Retro-Michael addition

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Sugarcane/soybean intercropping with reduced nitrogen addition enhances residue-derived labile soil organic carbon and microbial network complexity in the soil during straw decomposition

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Journal of Integrative Agriculture 2024年第12期23卷 4216-4236页

作者： Tantan Zhang Yali Liu Shiqiang Ge Peng Peng Hu Tang jianwu wang Key Laboratory of Agro-Environments in Tropics Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Eco-Circular Agriculture/College of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China

Sugarcane/soybean intercropping with reduced nitrogen addition is an important sustainable agricultural pattern that can alter soil ecological functions,thereby affecting straw decomposition in the ***,the mechanisms underlying changes in soil organic carbon(SOC)composition and microbial communities during straw decomposition under long-term intercropping with reduced nitrogen addition remain *** this study,we conducted an in-situ microplot incubation experiment with^(13)C-labeled soybean straw residue addition in a two-factor(cropping pattern:sugarcane monoculture(MS)and sugarcane/soybean intercropping(SB);nitrogen addition levels:reduced nitrogen addition(N1)and conventional nitrogen addition(N2))long-term experimental field *** results showed that the SBN1 treatment significantly increased the residual particulate organic carbon(POC)and residual microbial biomass carbon(MBC)contents during straw decomposition,and the straw carbon in soil was mainly conserved as *** addition changed the structure and reduced the diversity of the soil microbial community,but microbial diversity gradually recovered with decomposition *** straw decomposition,the intercropping pattern significantly increased the relative abundances of Firmicutes and *** addition,straw addition reduced microbial network complexity in the sugarcane/soybean intercropping pattern but increased it in the sugarcane monoculture ***,microbial network complexity remained higher in the SBN1 treatment than in the MSN1 *** general,the SBN1 treatment significantly increased the diversity of microbial communities and the relative abundance of microorganisms associated with organic matter decomposition,and the changes in microbial communities were mainly driven by the residual labile SOC *** findings suggest that more straw carbon can be sequestered in the soil under sugarcane/soybean intercropping with reduced nitrogen addition to ma

关键词： sugarcane/soybean intercropping ^(13)C-labeled straw labile SOC fractions microbial networks

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Fuzzy Simulation Human Intelligent Control System Design on Gyratory Breaker

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微计算机信息 2005年第1期21卷 11-12页

作者： Wen,Ruchun Zhao,Shuling Zhu,jianwu wang,Xiaoyan MechanicalandElectronicEngineeringSchoolatJiangxiUniversityofScienceandTechnology GanzhouJiangxiChina341000

In order to deal with the complex process that incurs serious time delay, enormous inertia and nonlinear problems, fuzzy simulation human intelligent control algorithm rules are established. The fuzzy simulation human intelligent controller and the hardware with the single-chip microcomputer are designed and the anti-interference measures to the whole system are provided.

关键词：模糊控制人工智能模拟控制旋转断路器单芯片微计算机

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Design, Synthesis and Biological Activity of Tetrazole-bearing Uric Acid Transporter 1 Inhibitors

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Chemical Research in Chinese Universities 2017年第1期33卷 49-60页

作者： CAI Wenqing LIU Wei XIE Yafei WU Jingwei LIU Yuqiang LIU Changying XU Weiren TANG Lida wang jianwu ZHAO Guilong School of Chemistry and Chemical Engineering Shandong University Jinan 250100 P. R. China Tianjin Key Laboratory of Molecular Design and Drug Discovery Tianjin Institute of Pharmaceutical Research Tianjin 300193 P. R. China

Systematic structure-activity relationship（SAR） exploration of a moderately active tetrazole-bearmg lesinurad-based hit If led to the discovery of a potent uric acid transporter I（URAT1） inhibitor li, which possessed a novel molecular skeleton and was 11-fold more potent than the parent lesinurad against human URAT1 in-vitro （IC50=0.66 gmol/L for li vs. 7.18 μmol/L for lesinurad）.

关键词： URAT1 inhibitor Structure-activity relationship Drug discovery Lesinurad Tetrazole Bioisostere

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Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine

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Frontiers of Agricultural Science and Engineering 2015年第1期2卷 90-99页

作者： Jianxiang XU Dengke PAN Jie ZHAO jianwu wang Xiaohong HE Yuehui MA Ning LI State Key Laboratory for Agrobiotechnology China Agricultural University Institute of Animal Sciences Chinese Academy of Agricultural Sciences

Myostatin is a transforming growth factor-beta family member that normally acts to limit skeletal muscle growth. Myostatin gene (MSTN) knockout (KO) mice show possible effects for the prevention or treatment of metabolic disorders such as obesity and type 2 diabetes. We applied chromatography and mass spectrometry based metabonomics to assess system-wide metabolic response of heterozygous MSTN KO (MSTN+/-) swine. Most of the metabolic data for MSTN+/- swine were similar to the data for wild type (WT) control swine. There were, however, metabolic changes related to fatty acid metabolism, glucose utilization, lipid metabolism, as well as BCAA catabolism caused by monoallelic MSTN depletion. The statistical analyses suggested that: (1) most metabolic changes were not significant in MSTN+/- swine compared to WT swine; (2) only a few metabolic properties were significantly different between KO and WT swine, especially for lipid metabolism. Significantly, these minor changes were most evident in female KO swine and suggested differences in gender sensitivity to myostatin.

关键词： myostatin transforming growth factor-beta family skeletal muscle metabolic disorders chromatography mass spectrometry metabonomics

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