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Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes

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Horticulture Research 2018年第1期5卷 418-430页

作者： Jieqiong Zhang Zichen Wu Fuchu Hu Lian Liu Xuming Huang Jietang Zhao huicong wang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of HorticultureSouth China Agricultural UniversityGuangzhouChina Guangdong Litchi Engineering Research Center College of HorticultureSouth China Agricultural UniversityGuangzhouChina Key laboratory of tropical fruit tree biology of Hainan Province Hainan Academy of Agricultural ScienceHaikou571100China Department of Life Sciences and Technology Yangtze Normal UniversityChongqingChina Shanghai Center for Plant Stress Biology(PSC) Shanghai Institutes of Biological SciencesChinese Academy of SciencesShanghai201602China

Cell wall invertase(CWIN)are known to play important roles in seed ***,most reports to date have focused on a single gene family member,and have mainly investigated CWIN functions during the filling stage of seed *** this study,we found significant lower levels of CWIN protein and activity associated with seed abortion in the Litchi chinensis cultivar“Nuomici.”We identified five litchi CWIN genes and observed that the expression of LcCWIN5 was limited to the flower tissues and decreased sharply with fruit *** of LcCWIN5 expression before 28 DAA(cell division stage)resulted in perturbed liquid endosperm development,smaller seeds,and higher seed abortion rate,while silencing after 28 DAA(filling stage)had no effect on seed *** contrast,LcCWIN2 was mostly expressed in the funicle and seed coat,and increased with fruit *** LcCWIN2 expression and CWIN activity during early seed filling coincided with smaller seeds in the cultivar“Feizixiao.”Silencing of LcCWIN2 caused a reduction in the seed size without inducing seed *** propose that CWIN activity in seed maternal tissues during cell division stage is likely due to LcCWIN5 expression,which regulates early seed *** the other hand,CWIN activity during the filling stage is due to the expression of LcCWIN2,which may promote carbon import by creating a sucrose *** LcCWIN5 expression,but much lower CWIN activity,detected in the funicle of“Nuomici”is consistent with post-translational regulation.

关键词： development. impaired sharply

Single-cell transcriptomic analysis reveals a systemic immune dysregulation in COVID-19-associated pediatric encephalopathy

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Signal Transduction and Targeted Therapy 2023年第11期8卷 5429-5443页

作者： Yi wang Laurence Don Wai Luu Shuang Liu Xiong Zhu Siyuan Huang Fang Li Xiaolan Huang Linying Guo Jin Zhang Haiyan Ge Yuanyuan Sun Yi Hui Yanning Qu huicong wang Xiaoxia wang Weilan Na Juan Zhou Dong Qu Jun Tai Experimental Research Center Capital Institute of PediatricsBeijing100020P.R.China School of Life Sciences University of Technology SydneySydneyAustralia Department of Critical Medicine Children’s Hospital Affiliated Capital Institute of PediatricsBeijing100020P.R.China Central&Clinical Laboratory of Sanya People’s Hospital SanyaHainan572000P.R.China Department of Otorhinolaryngology Head and Neck Surgery Children’s Hospital Affiliated Capital Institute of PediatricsBeijing100020P.R.China

Unraveling the molecular mechanisms for COVID-19-associated encephalopathy and its immunopathology is crucial for developing effective ***,we utilized single-cell transcriptomic analysis and integrated clinical observations and laboratory examination to dissect the host immune responses and reveal pathological mechanisms in COVID-19-associated pediatric *** found that lymphopenia was a prominent characteristic of immune perturbation in COVID-19 patients with encephalopathy,especially those with acute necrotizing encephalopathy(AE).This was characterized a marked reduction of various lymphocytes(e.g.,CD8^(+)T and CD4^(+)T cells)and significant increases in other inflammatory cells(e.g.,monocytes).Further analysis revealed activation of multiple cell apoptosis pathways(e.g.,granzyme/perforin-,FAS-and TNF-induced apoptosis)may be responsible for lymphopenia.A systemic S100A12 upregulation,primarily from classical monocytes,may have contributed to cytokine storms in patients with AE.A dysregulated type I interferon(IFN)response was observed which may have further exacerbated the S100A12-driven inflammation in patients with *** COVID-19 patients with AE,myeloid cells(e.g.,monocytic myeloid-derived suppressor cells)were the likely contributors to immune ***,the immune landscape in COVID-19 patients with encephalopathy,especially for AE,were also characterized by NK and T cells with widespread exhaustion,higher cytotoxic scores and inflammatory response as well as a dysregulated B cell-mediated humoral immune *** together,this comprehensive data provides a detailed resource for elucidating immunopathogenesis and will aid development of effective COVID-19-associated pediatric encephalopathy treatments,especially for those with AE.

关键词： encephalopathy analysis likely

Development of ＂Purple Endosperm Rice＂ by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System

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Molecular Plant 2017年第7期10卷 918-929页

作者： Qinlong Zhu Suize Yu Dongchang Zeng Hongmei Liu huicong wang Zhongtang Yang Xianrong Xie Rongxin Shen Jiantao Tan Heying Li Xiucai Zhao Qunyu Zhang Yuanling Chen Jingxing Guo Letian Chen Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology Guangdong Provincial Higher Education Institutions Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China School of Biology and Engineering Guizhou Medical University Guangzhou 510642 China Physioiogical Laboratory for South China Fruits College of HorLiculture South China Agricultural University Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms Guangzhou 510642 China

Anthocyanins have high antioxidant activities, and engineering of anthocyanin biosynthesis in staple crops, such as rice （Oryza sativa L.）, could provide health-promoting foods for improving human health. However, engineering metabolic pathways for biofortification remains difficult, and previous attempts to engineer anthocyanin production in rice endosperm failed because of the sophisticated genetic regulatory network of its biosynthetic pathway. In this study, we developed a high-efficiency vector system for transgene stacking and used it to engineer anthocyanin biosynthesis in rice endosperm. We made a construct containing eight anthocyanin-related genes （two regulatory genes from maize and six structural genes from Coleus） driven by the endosperm-specific promoters,plus a selectable marker and a gene for marker excision. Transformation of rice with this construct generated a novel biofortified germplasm ＂Purple Endosperm Rice＂（called ＂Zijingmi＂ in Chinese）, which has high anthocyanin contents and antioxidant activity in the endosperm. This anthocyanin production results from expression of the transgenes and the resulting activation （or enhancement） of expression of 13 endogenous anthocyanin biosynthesis genes that are silenced or expressed at low levels in wild-type rice endosperm. This study provides an efficient, versatile toolkit for transgene stacking and demonstrates its use for successful engineering of a sophisticated biological pathway, suggesting the potential utility of this toolkit for synthetic biology and improvement of agronomic traits in plants.

关键词： biofortification metabolic engineering multi-transgene stacking anthocyanin biosynthesis rice endosperm

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压电材料中多个孔边径向裂纹的动力相互作用

振动与冲击 2016年第16期35卷 176-180,225页

作者：李冬王慧聪宋天舒河北交通职业技术学院土木工程系石家庄050091 哈尔滨工程大学航天与建筑工程学院哈尔滨150001

采用Green函数法对压电材料中多个孔边径向裂纹在SH波作用下的相互作用问题进行了研究。首先利用复变函数方法构造出具有多个半圆形凹陷的半无限压电介质的位移Green函数和电场Green函数,然后采用裂纹"切割"技术构造孔边径向裂纹,根据... 详细信息

采用Green函数法对压电材料中多个孔边径向裂纹在SH波作用下的相互作用问题进行了研究。首先利用复变函数方法构造出具有多个半圆形凹陷的半无限压电介质的位移Green函数和电场Green函数,然后采用裂纹"切割"技术构造孔边径向裂纹,根据界面上的位移和应力连续性条件建立求解问题的第一类Fredholm定解积分方程。最后作为算例,给出了裂纹尖端动应力强度因子随缺陷几何尺寸、材料物理参数和入射波频率的变化特征图并进行了讨论。

关键词：压电材料孔边径向裂纹 Green 函数动应力强度因子 SH 波散射 dynamic stress intensity factor(DSIF)

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NAC转录因子在果实成熟中的调控作用

果树学报 2023年第7期40卷 1455-1470页

作者：卓茂根王惠聪华南农业大学农业农村部华南地区园艺作物生物学与种质创制重点实验室·园艺学院广东省荔枝工程中心广州510640

NAC转录因子是植物中最大的特异性转录因子家族之一,其依赖由高度保守的N端结构域和高度变异的C端转录调控结构域组成独特的NAC结构域发挥功能,在果实成熟的调控中发挥着重要的作用。在介绍NAC转录因子的结构特征及不同物种NAC基因的成... 详细信息

NAC转录因子是植物中最大的特异性转录因子家族之一,其依赖由高度保守的N端结构域和高度变异的C端转录调控结构域组成独特的NAC结构域发挥功能,在果实成熟的调控中发挥着重要的作用。在介绍NAC转录因子的结构特征及不同物种NAC基因的成员与分类的基础上,总结了近年来NAC转录因子在果实成熟过程中的积极作用,包括促进果实软化影响果实质地,促进叶绿素降解及类胡萝卜素、类黄酮和花色苷的合成决定果实着色、调控糖分积累影响果实糖酸比例以及促进多种芳香化合物合成积累形成果实特有风味、果实脱涩等方面,并阐述了NAC转录因子与内源激素特别是乙烯和ABA交互在调控果实成熟中的重要作用。总结了NAC转录因子调控果实成熟的潜在机制以及影响NAC表达的因素与分子通路,旨在为在果实成熟性状遗传改良与调控技术研发提供重要参考。

关键词：果实成熟 NAC转录因子软化着色糖分积累芳香化合物合成

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不同土体中单排弹性桩对水平向剪切波的隔离

地震工程与工程振动 2015年第2期35卷 78-85页

作者：李冬王慧聪宋天舒河北交通职业技术学院土木工程系河北石家庄050091 哈尔滨工程大学航天与建筑工程学院黑龙江哈尔滨150001

采用Green函数法和"契合"思想研究不同土体中界面上单排弹性桩对SH波的隔离问题。首先构造适于本问题的位移Green函数,然后利用"契合"思想并结合界面上的应力和位移连续条件,将问题转化为一组第一类Fredholm积分方程,得到了弹性桩后位... 详细信息

采用Green函数法和"契合"思想研究不同土体中界面上单排弹性桩对SH波的隔离问题。首先构造适于本问题的位移Green函数,然后利用"契合"思想并结合界面上的应力和位移连续条件,将问题转化为一组第一类Fredholm积分方程,得到了弹性桩后位移和透射系数的解析表达式,并进行数值求解。最后作为算例,给出了等间距单排8个弹性圆柱桩对SH波的隔离效果图,分析了剪切弹性模量、桩间距等对桩后隔离效果的影响规律。通过分析曲线得出:隔振的最佳区域并不是弹性桩中心线后的近距离范围内,而是位于弹性桩后的一定距离上;当桩间距s/R<1时,桩后区域取得最佳隔振效果。

关键词：弹性桩水平向剪切波隔振无量纲位移透射系数

基于充分降维方法的河南省农业综合竞争力研究

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河南农业大学学报 2020年第6期54卷 1074-1080页

作者：王慧聪费宇云南财经大学统计与数学学院云南昆明650221

本文选取国家统计局2008年、2013年和2018年的各省市农业相关数据,利用充分降维方法对河南省的农业综合竞争力进行研究。结果表明,近10年来河南省农业综合竞争力在全国始终处于1类水平,但与江苏和山东等省市仍有差距。今后的农业发展应... 详细信息

本文选取国家统计局2008年、2013年和2018年的各省市农业相关数据,利用充分降维方法对河南省的农业综合竞争力进行研究。结果表明,近10年来河南省农业综合竞争力在全国始终处于1类水平,但与江苏和山东等省市仍有差距。今后的农业发展应着重于进一步提升农业技术水平,逐步向绿色农业转变,提高农业抗风险能力等。

关键词：农业综合竞争力充分降维聚类分析河南省

儿童特应性皮炎严重程度与血清25-羟维生素D和总IgE水平的相关性

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中国麻风皮肤病杂志 2019年第2期35卷 86-88页

作者：张媛王慧丛颜敏胜利油田中心医院山东东营257000

目的:明确儿童特应性皮炎患者特应性皮炎严重程度与血清25-羟维生素D和IgE水平的相关性。方法:参考SCORAD评分法评估60例特应性皮炎患者疾病严重程度,并检测特应性皮炎患者及55例对照组血清25-羟基维生素D3水平以及特应性皮炎患者总IgE... 详细信息

目的:明确儿童特应性皮炎患者特应性皮炎严重程度与血清25-羟维生素D和IgE水平的相关性。方法:参考SCORAD评分法评估60例特应性皮炎患者疾病严重程度,并检测特应性皮炎患者及55例对照组血清25-羟基维生素D3水平以及特应性皮炎患者总IgE水平。结果:特应性皮炎组患者血清25-羟维生素D水平(16.13±6.68)ng/mL低于对照组(19.81±8.24)ng/mL,差异有统计学意义(P0.05)。结论:AD患者血清25-羟基维生素D与AD严重程度呈负相关。

关键词：特应性皮炎疾病严重程度指数 25-羟维生素D IgE

物质文明与精神文明协调发展水平测度及影响因素分析

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统计与决策 2024年第13期40卷 34-39页

作者：朱喜安王慧聪中南财经政法大学统计与数学学院武汉430073

文章基于我国31个省份2011—2021年的面板数据,分别构建物质文明与精神文明发展水平指标体系,利用熵值法进行测度,借助耦合协调度模型和离差系数协调度模型对物质文明与精神文明协调发展水平进行测度,并结合动态空间杜宾模型对影响二者... 详细信息

文章基于我国31个省份2011—2021年的面板数据,分别构建物质文明与精神文明发展水平指标体系,利用熵值法进行测度,借助耦合协调度模型和离差系数协调度模型对物质文明与精神文明协调发展水平进行测度,并结合动态空间杜宾模型对影响二者协调发展水平的因素进行分析。结果表明:第一,样本考察期内,我国大部分省份物质文明与精神文明协调发展水平有一定程度的提升,东部地区省份协调发展水平提升较慢,中部和西部地区省份协调发展水平提升较快,三大地区整体上呈现东部高、中部居中、西部低的分布格局。第二,物质文明与精神文明协调发展水平具有时间上的叠加效应和空间上的辐射带动效应。第三,城镇化水平、人口密度和老龄化水平对协调发展水平有显著正向影响,而少儿比率具有一定的抑制作用。

关键词：物质文明精神文明耦合协调动态空间杜宾模型

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