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Expression Optimization and Characterization of the Catalytic Domain of Human MT3-MMP

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Chemical Research in Chinese Universities 2006年第2期22卷 129-133页

作者： SHI Xiu-juan JIN Feng-hai wang hui-ling YANG Jin-gang wang Zhi-yong FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering the Ministry of Education School of Pharmacy College of Life Science Jilin University Changchun 130021 P. R. China

Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as therapeutic agents in treating a number of diseases including cancer, cardiovascular diseases and arthritis. Attempts have been made to develop MMPIs. Recombinant MMPs have been used to screening MMPs in vitro assays. In this work, we report the expression of MMP-16 in E. coli and the characterization of the recombinant MMP-16 with a commonly used MMP substrate DQ-gelatin.

关键词： Matrix metalloproteinase(MMP) Protein expression Catalytic domain MMP-16

Inhibition of Metalloproteinase Activity by Chinese Formulations Used to Treat Inflammatory Diseases

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Chemical Research in Chinese Universities 2006年第2期22卷 245-247页

作者： JIN Feng-hai wang hui-ling ZHAO Shu-hua YANG Jin-gang FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering the Ministry of Education Jilin University Changchun 130021 P. R. China School of Pharmacy Jilin University Changchun 130021 P. R. China College of Life Science Jilin University Changchun 130021 P. R. China The China-Japan Union Hospital Jilin University Changchun 130021 P. R. China

Matrix metalloproteinases （MMPs） are known to be involved in a number of pathological processes including cancer, atherosclerosis, arthritis and neurodegenerative disease among others. The drive to develop MMP inhibitors as therapeutics has been put forward for years by pharmaceutical companies as well as academicians. In an attempt to screen for MMP inhibitors from Traditional Chinese Medicines （TCMs） , a number of Chinese formulations used to treat inflammatory diseases such as nephritis and hepatitis have been studied. Strong inhibitory effects of three Chinese formulations toward the activity of MMP-16 have been discovered. These results suggest that these anti-inflammatory medicines contain some unknown MMP inhibitory compound（s） and provide reasonable molecular mechanisms for their theraoeutic effects.

关键词： Matrix metalloproteinase (MMP) Matrix metalloproteinase inhibitor ( MMPI ) Traditional Chinese Medicine(TCM) Herbal formulation

Inhibition of Matrix Metalloproteinases Activities by Luteolin

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Chemical Research in Chinese Universities 2009年第6期25卷 895-898页

作者： JI Hai-tao SHI Xiu-juan HONG Yuan wang Ye wang hui-ling wang Zhi-yong FANG Xue-xun College of Life Science Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

Matrix metalloproteinases（MMPs） are a family of zinc dependent enzymes that can degrade the components of the extracellular matrix（ECM） and basement membranes. Because MMPs play roles in many important physiological and pathological processes, many MMP inhibitors（MMPls） have been developed with the aim of interfering in and treating diseases. The authors conclude via a fluorescence based assay in vitro that luteolin inhibits the enzymatic activities of MMP-2, MMP-7, MMP-9, MMP-14, and MMP-16. This compound most specifically inhibits MMP-7 of all the MMPs tested. Finally, we used molecular modeling to dock luteolin with MMPs and revealed the binding mode of the luteolin-MMP interaction. Our results suggest that luteolin may exert its therapeutic effects via MMP inhibition.

关键词： Matrix metalloproteinase(MMP) MMP inhibitor(MMPI) Natural product Luteolin

Subchronic Oral Toxicity Evaluation of Sodium Dehydroacetate: A 90-day Repeated Dose Study in Rats

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Biomedical and Environmental Sciences 2022年第4期35卷 296-311页

作者： FANG Jin LIU Hai Bo ZHI Yuan FENG Yong Quan wang hui ling CUI Wen Ming ZHANG Ji Yue wang Hua Li YU Zhou JIA Xu Dong NHC Key Laboratory of Food Safety Risk Assessment Chinese Academy of Medical Science Research Unit(2019RU014)China National Center for Food Safety Risk AssessmentBeijing 100021China

Objective The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate(DHA-Na)and to determine the point of departure(POD),which is a critical factor in the establishment of an acceptable dietary *** DHA-Na was administered once daily by gavage to Sprague–Dawley rats at dose levels of 0.0,31.0,62.0,and 124.0 mg/kg BW per day for 90 days,followed by a recovery period of 4 weeks in the control and 124.0 mg/kg BW per day *** outcome parameters were mortality,clinical observations,body weights,food consumption,hematology and clinical biochemistry,endocrine hormone levels,and ophthalmic,urinary,and histopathologic *** benchmark dose(BMD)approach was applied to estimate the *** Significant decreases were found in the 62.0 and 124.0 mg/kg BW groups in terms of the body weight and food utilization rate,whereas a significant increase was found in the thyroid stimulating hormone levels of the 124.0 mg/kg BW ***,the 95%lower confidence limit on the BMD of 51.7 mg/kg BW was modeled for a reduction in body *** The repeated-dose study indicated the slight systemic toxicity of DHA-Na at certain levels(62.0 and 124.0 mg/kg BW)after a 90-day oral exposure.

关键词： Sodium dehydroacetate Sub-chronic toxicity 90-day repeated oral dose test Benchmark dose Point of departure

Hepatoprotective role of ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice

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World Journal of Gastroenterology 2002年第4期8卷 728-733页

作者： Guo-Liang Zhang Ye-Hong wang Wei Ni hui-ling Teng Zhi-Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijing 100083,China Department of Pharmacology School of Basic Medical Sciences Beijing University Beijing 100083 China. yuankui@***

AIM: To examine the effect of ganoderma lucidumpolysaccharide (GLP) on the immune liver injuryinduced by BCG infection, and investigate therelationship between degrees of hepatic damage andNO production in ***: Immune hepatic injury was markedlyinduced by BCG-pretreatment (125 ***-1, 2-week, iv)or by BCG-pretreatment plus lipopolysaccharide (LPS,125 μ***-1, 12-hour, iv) in mice in *** induced by BCG-pretreated plus inflammatorycytokines mixture (CM), which was included TNF-α, IL1β, IFN-γ and LPS in culture medium in *** of GLP was performed by oral orincubating with culture medium at immune stimulisimultaneity. Liver damage was determined by activityof alanine aminotransferase (ALT) in serum and inhepatocytes cultured supernatant, by liver weightchanges and histopathological examination. NOproduction in the cultured supematant was determinedby the Griess reaction. Moreover, inducible nitric oxidesynthase (iNOS) protein expression was alsoexaminated by immunohistochemi1cal ***: Immune hepatic injury was markedly inducedby BCG or BCG plus inflammatory cytokines in BALB/cmice in vivoand in vitro. Under BCG-stimulated condition,augment of the liver weight and increase of the serum/supernatant ALT level were observed, as well asgranuloma forming and inflammatory cells soakage wereobserved by microscopic analysis within liver ***, NO production was also increased by BCG or/and CH stimuli in the culture supernatant, and a lot ofiNOS positive staining was observed in BCG-prestimulated hepatic sections. Application of GLPsignificantly mitigated hepatic tumefaction, decreasedALT enzyme release and NO production in serum/supernatant, improved the pathological changes ofchronic and acute inflammation induced by BCG-stimuliin mice. Moreover, the immunohistochemical resultshowed that GLP inhibited iNOS protein expression inBCG-immune hepatic damage ***: The present study indicates that NOparti

关键词：灵芝多糖卡介苗肝损伤肝脏保护动物实验作用机制

5 -Lipoxygenase and cysteinyl leukotriene receptors in brain injury and repair

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中国药理通讯 2005年第4期22卷 32-33页

作者： Er - Qing Wei Wei - Ping Zhang Meng - ling wang ling - hui Zeng Department of Pharmacology School of Medicine Zhejiang University Hangzhou 310031

Three kinds of mesenchymal stem cells:differentiating into neuron-like cells

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中国组织工程研究 2018年第33期22卷 5356-5360页

作者： Yang Ying Xu Qian wang hui-ling Yao Min-xiu Department of Endocrinology Second Affiliated Hospital of Qingdao University Medical SchoolQingdao 266042Shandong ProvinceChina

BACKGROUND:Stem cells,characterized with convenient collection,wide sources and great potential,have been developed in the cell *** most commonly used stem cells include bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem ***:To compare the biological changes in bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells after co-cultured with nerve ***:Human bone marrow mesenchymal stem cells,human placental mesenchymal stem cells and human umbilical cord blood mesenchymal stem cells were isolated and cultured in *** morphological changes of mesenchymal stem cells differentiated into nerve cells were observed by co-culture with Transwell culture *** expression of neuron-specific enolase was detected after *** AND CONCLUSION:In the co-culture system,these three kinds of mesenchymal stem cells gradually formed a star-like shape and were ***,all the cells were positive for neuron-specific *** findings reveal that the microenvironment provided by nerve cells can induce and promote the differentiation of three kinds of mesenchymal stem cells into neuron-like cells.

关键词： Mesenchymal Stem Cells Coculture Techniques Tissue Engineering

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冬瓜山铜矿隔离矿柱开采的地压危害分析

长江科学院院报 2014年第9期31卷 88-92页

作者：汪令辉铜陵有色金属股份集团有限公司冬瓜山铜矿安徽铜陵244031

针对冬瓜山铜矿厚大矿体盘区隔离矿柱开采过程中的地压危害问题,应用数值模拟方法并采取微震监测等手段进行分析研究。结果表明:开采过程中发生岩爆的可能性很低;由于隔离矿柱开采的技术条件劣化很多,位移和塑性区域明显增大,应该对单... 详细信息

针对冬瓜山铜矿厚大矿体盘区隔离矿柱开采过程中的地压危害问题,应用数值模拟方法并采取微震监测等手段进行分析研究。结果表明:开采过程中发生岩爆的可能性很低;由于隔离矿柱开采的技术条件劣化很多,位移和塑性区域明显增大,应该对单个开挖单元的空间体量加以控制。对模拟的54#隔离矿柱而言,开挖空间高度不宜超过50 m,长度不宜超过48 m,采用间隔开挖顺序能够降低工程岩体的应力集中度,对工程稳定有利。

关键词：岩体开挖地压危害微震监测数值模拟

The logic analyses of heat transfer process between solids

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Journal of Energy and Power Engineering 2007年第1期1卷 29-33,40页

作者： CHEN Jin wang hui-ling ZHOU Zheng-xin HONG Xiao-ou School of Electronic ＆ Electrical Engineering Shanghai Second Polytechnic University Shanghai 201209 China Institute of Refrigeration and Cryogenics Huazhong University of Science and Technology Wuhan 430074 China

The thermal switch algebra method and theory, which is used as a power tool of logic analyses of heat transfer process, is discussed in this paper, it was a practical application of pan-logic algebra in the heat science. As an example of heat switch algebra application, the logic algebra model of interfacial thermal resister between Bi-2223 and AIN were built at the range from 30K to 200K according to the thermal switch algebra theory. The computer simulation results were agreed greatly with experiment data, the error is less that 5%. The design and analyses of digit heat routes can be described by the thermal switch algebra as well.

关键词： heat transfer thermal switch algebra thermal resister