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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu jie mu yu Leng Lei Ma yu Zheng Ruizhi Li Yin wang Peifeng Li hai Zhu Dong wang jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

Rate Constants for Reaction Between Hydroxyl Radical and Dimethyl Sulfide Under Real Atmospheric Condition

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Chinese Journal of Chemical Physics 2008年第5期21卷 407-414页

作者： hai-tao wang Chang-jiang Hu yu-jing mu yu-jie zhang Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China

The rate constants of the reaction between hydroxyl radical （OH-） and dimethyl sulfide （DMS） were investigated by using the relative methods in air, N2, and 02. Strong influences of ground state oxygen O（3p） on DMS consumption were found by the photolysis of HONO and CH3ONO as OH. sources, and the rate constants obtained in these systems varied significantly. The rate constants of the reaction between DMS and OH- （generated by photolysis of H2 O2） at room temperature were 8.56 × 10^-12, 11.31× 10^-12, and 4.50× 10^-12 cm3/（molecule.s）, in air, O2, and N2, respectively. The temperature dependence of the rate constants for OH- with DMS over the temperature range of 287-338 K was also investigated in nitrogen and air, and the Arrhenius expression was obtained as follows： kaire=（7.24±0.28）× 10^-13exp[（770.7±E97.2）/T], kN2 =（3.40±0.15） × 10^-11 exp[-- （590.3±165.9）/T].

关键词： Dimethyl sulfide Hydroxyl radical Ground state oxygen Kinetics

A Trojan horse biomimetic delivery system using mesenchymal stem cells for HIF-1α siRNA-loaded nanoparticles on retinal pigment epithelial cells under hypoxia environment

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International Journal of Ophthalmology(English edition) 2022年第11期15卷 1743-1751页

作者： Lei zhang jie-jing Yan hai-Yan wang mu-Qiong Li Xi-Xi wang Li Fan yu-Sheng wang Shaanxi Eye Hospital Xi'an People's Hospital(Xi'an Fourth Hospital)Xi'an 710004Shaanxi ProvinceChina Department of Ophthalmology Xijing HospitalXi'an 710032Shaanxi ProvinceChina Ophthalmology Department Xi'an No.1 Hospitalthe First Affiliated Hospital of Northwest UniversityXi'an 710002Shaanxi ProvinceChina Department of Pharmaceutical Chemistry and Analysis School of Pharmacy Air Force Medical University Xi'an 710032Shaanxi ProvinceChina Department of Mathematics and Statistics University of Arkansas at Little RockLittle RockAR 72204USA

AIM: To demonstrate the feasibility of mesenchymal stem cell(MSC)-mediated nano drug delivery, which was characterized by the “Trojan horse”-like transport of hypoxiainducible factor-1α small interfering RNA(HIF-1α si RNA) between MSCs and retinal pigment epithelial cells(RPE) under hypoxia ***: Plasmid and lentivirus targeting the human HIF-1α gene were designed and constructed. HIF-1α si RNA was encapsulated into poly(lactic-co-glycolic acid) nanoparticles(PLGA-NPs) through the water-in-oil-in-water(w/o/w) multiple emulsion technique. The effect of PLGANPs uptake on the expression of HIF-1α m RNA was tested in RPE cells by real-time quantitative polymerase chain reaction(q PCR) and additional transfected conditions were used as control, including lentivirus group, nude plasmid group and blank PLGA group. MSCs were transfected with the NPs and the transfection efficacy was evaluated by flow cytometry. Transwell co-culture system of transfected MSCs and RPE cells was constructed under hypoxia environment. The effects of MSC-loaded HIF-1α si RNA PLGA-NPs on proliferation, apoptosis, and migration of RPE cells were then evaluated. The effect of transfected MSCs on HIF-1α expression of RPE cells was analyzed by using q PCR at the time points 24h, 3d, and ***: The average diameter of PLGA-NPs loaded with HIF si RNA was 314.1 nm and the zeta potential was-0.36 m V. The transfection efficiency of PLGA-NPs was 67.3%±5.2% into MSCs by using flow cytometry. Compared with the lentivirus group, the PLGA-NPs loaded with HIF-1α si RNA can effectively reduce the expression of HIF-1α m RNA up to 7d in RPE(0.63±0.05 at 7d, P<0.001). In the Transwell co-culture system of transfected MSCs and RPE, the abilities of proliferation(2.34±0.17, 2.40±0.28, 2.47±0.24 at 48h, F=0.23, P=0.80), apoptosis(14.83%±2.43%, 12.94%±2.19%, 12.39%±3.21%;F=0.70, P=0.53) and migration(124.5±7.78, 119.5±5.32, 130±9.89, F=1.33, P=0.33) of the RPE cells had no differences between MSClo

关键词： hypoxia mesenchymal stem cells poly(lactic-co-glycolic acid)nanoparticles hypoxia-inducible factor-1α retinal pigment epithelial cells

Interface optimization of graphene paper-Cu composite prepared by electrodeposition

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Journal of Iron and Steel Research International 2024年第1期31卷 64-73页

作者： jing Guo hai-jing wang Shuang-juan Liu Peng zhang jing-yu Tang Wei-yang zhang Han-jie Guo Peng-cheng wang Cai Meng School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China Institute of High Energy Physics Chinese Academy of SciencesBeijing100049China Advanced Technology and Materials Co. Ltd.Beijing100083China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei230027AnhuiChina

A room-temperature electrodeposition method with an organic electrolyte was developed to fabricate a HNO3-pretreated graphene paper Cu(GP'-Cu)*** improve the interfacial bonding of GP'-Cu composite,magnetron sputtering technology was used to create a"sandwich"structural gradient GP'-Cu *** selection of the intermediate transition layer metal was based on two-dimensional *** electron microscopy,X-ray photoelectron spectroscopy,and other analytical methods confirmed that the addition of an intermediate transition metal(Cr,Ni)layer reduced the gap distance and enhanced the interfacial bonding of the GP'and Cu deposited *** GP'-Ni-Cu composite exhibited the largest increase in tensile strength and *** addition,it had the highest thermal diffusivity and elongation at break among the GP'-Cu,GP'-Cr-Cu and GP'-Ni-Cu composites.

关键词： Graphene paper Two-dimensional disregistry Magnetron sputtering Metal composite

Combined fascia iliaca compartment block and monitored anesthesia care for geriatric patients with hip fracture: Two case reports

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World Journal of Clinical Cases 2021年第27期9卷 8268-8273页

作者： Li Zhan yu-jie zhang jing-Xian wang Department of Anesthesiology Lu’an Affiliated Hospital of Anhui Medical UniversityAnhui Medical UniversityLu’an 237005Anhui ProvinceChina

BACKGROUND Major hip surgery usually requires neuraxial or general anesthesia with tracheal intubation and may be supplemented with a nerve block to provide intraoperative and postoperative pain *** SUMMARY This report established that hip surgical procedures can be performed with a fascia iliaca compartment block(FICB)and monitored anesthesia care(MAC)while avoiding neuraxial or general *** was a preliminary experience with two geriatric patients with hip fracture,American Society of Anesthesiologists status III,and with many *** patient could be operated on within 48 h after *** general anesthesia and neuraxial anesthesia were high-risk procedures and had ***,we chose nerve block combined with a small amount of *** analgesia was provided by single-injection ultrasound-guided *** intravenous sedation was *** exposure was satisfactory,and neither patient complained of any symptoms during the *** This report showed that hip surgery for geriatric patients can be performed with FICB and MAC,although complications and contraindications are *** anesthetic program was accompanied by stable respiratory and circulatory system responses and satisfactory analgesia while avoiding the adverse effects and problems associated with either neuraxial or general anesthesia.

关键词： Fascia iliaca compartment block Monitored anesthesia care Geriatric Hip fracture Ultrasound-guided Case report

miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes

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Hepatobiliary & Pancreatic Diseases International 2019年第4期18卷 360-365页

作者： jing-jie wang Yi-Tong zhang yu Jen Tseng Jun zhang Department of Digestive Diseases Huashan Hospital Fudan University

Background:Non-alcoholic fatty liver disease(NAFLD)is one of the most prevalent chronic liver ***,the exact pathogenesis of NAFLD remains to be *** the association with tumors and cardiovascular diseases,the role of miR-222 in NAFLD remains *** present study was to investigate the role of miR-222 in ***:Wild-type C57BL/6 mice were fed a high-fat diet for 12 weeks to induce *** human liver cell line(L02)was cultured with free fatty acid(FFA)-containing medium to stimulate cell *** mRNA levels of miR-222 and acyl Coenzyme A xidase 1(ACOX1)were detected by quantitative-PCR(Q-PCR).The prediction of ACOX1 as the target gene for miR-222 was conducted via *** overexpression or inhibition of miR-222 was mediated by miR-222 mimics or antagomir,and intracellular triglyceride levels were measured using a triglyceride *** reporter assays verified ACOX1 as the target gene for ***:miR-222 was significantly elevated in both the in vivo and in vitro NAFLD *** of miR-222 significantly increased triglyceride content in the L02 cells,while inhibition of miR-222 expression restricted the accumulation of *** of miR-222 significantly inhibited ACOX1 *** transfection assays verified that ACOX1 3-UTR luciferase reporter activity could be inhibited by miR-222 ***:The present study suggested that miR-222 promotes the accumulation of triglycerides by inhibiting ACOX1.

关键词： ACOX1 Hepatocyte steatosis miR-222 NAFLD β-hydroxybutyrate

Feedback on a shared big dataset for intelligent TBM Part Ⅱ:Application and forward look

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Underground Space 2023年第4期11卷 26-45页

作者： Jian-Bin Li Zu-yu Chen Xu Li Liu-jie jing yun-Pei zhang Hao-Han Xiao Shuang-jing wang Wen-Kun Yang Lei-jie Wu Peng-yu Li hai-Bo Li Min Yao Li-tao Fan China Railway Group Co.Ltd Beijing 100089China Department of Geotechnical Engineering China Institute of Water Resources and Hydropower ResearchBeijing 100038China Key Laboratory of Urban Underground Engineering Ministry of EducationBeijing Jiaotong UniversityBeijing 100044China China Railway Engineering Equipment Group Co. LtdZhengzhouHenan 450016China School of Civil Engineering Southeast UniversityNanjing 211189China Xi’an University of Technology Xi’an 710048China

This review discusses the application scenarios of the machine learning-supported performance prediction and the optimization effi-ciency of tunnel boring machines(TBMs).The rock mass quality ratings,which are based on the Chinese code for geological survey,were used to provide"labels"suitable for supervised *** a result,the generation of machine prediction for rock mass grades reason-ably agreed with the ground truth documented in geological *** contrast,the main operational parameters,i.e.,thrust and torque,can be reasonably predicted based on historical ***,18 collapse sections of the Yinsong project have been successfully predicted by several *** studies on the selection of the optimal penetration rate and cost were *** review also presents a summary of the main achievements in response to the initiatives of the Lotus Pool Contest in *** the first time,large and well-documented TBM performance data has been shared for joint scientific ***,the review discusses the technical problems that require further study and the perspectives in the future development of intelligent TBM construction based on big data and machine learning.

关键词： TBM performance prediction TBM rock mass quality rating TBM-supported machine learning Rock mass classification ensemble Tunnel collapse

Catalytically active atomically thin cuprate with periodic Cu single sites

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National Science Review 2023年第1期10卷 226-235页

作者： Huimin Yang Shibo Xi Na Guo mu wang Lingmei Liu Pin Lyu Xiaolong yu jing Li Haomin Xu Xiao hai Zejun Li Xinzhe Li tao Sun Xiaoxu Zhao yu Han Wei yu jie Wu Chun zhang Honghan Fei Ming Joo Koh Jiong Lu Department of Chemistry National University of Singapore Institute of Chemical and Engineering Sciences Department of Physics National University of Singapore Advanced Membranes and Porous Materials Center King Abdullah University of Science and Technology SDU-ANU Joint Science College Shandong University School of Materials Science and Engineering Peking University Centre for Advanced 2D Materials and Graphene Research Centre National University of Singapore Department of Chemistry Tongji University

Rational design and synthesis of catalytically active two-dimensional(2D) materials with an abundance of atomically precise active sites in their basal planes remains a great ***,we report a ligand exchange strategy to exfoliate bulk [Cu4(OH)6] [O3S(CH2)4SO3] cuprate crystals into atomically thin 2D cuprate layers([Cu2(OH)3]+).The basal plane of 2D cuprate layers contains periodic arrays of accessible unsaturated Cu(Ⅱ) single sites(2D-CuSSs),which are found to promote efficient oxidative Chan-Lam *** me chanistic studies reveal that the reactions proceed via coordinatively unsaturated CuO4(Ⅰ) single sites with the formation of Cu(Ⅰ) species in the rate-limiting step,as corroborated by both operando experimental and theoretical *** robust stability of 2D-CuSSs in both batch and continuous flow reactions,coupled with their recyclability and good performance in complex molecule derivatization,render 2D-CuSSs attractive catalyst candidates for broad utility in fine chemical synthesis.

关键词： exfoliation nanosheet heterogeneous catalysis

Sesquiterpenes and prenylated C_6–C_3 compounds from the stems of Illicium henryi

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Chinese Chemical Letters 2015年第12期26卷 1538-1541页

作者： Peng-yu Zhuang Gui-jie zhang Xiao-jing wang Yan zhang Shi-Shan yu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

One new sesquiterpene compound, namely, illihenlactone A（1）, and one new prenylated C6–C3 compound, illihenryione H（2）, along with three known sesquiterpenes（3–5） were isolated from the stems of Illicium henryi. The structures of 1 and 2 were elucidated by spectroscopic evidence including NMR, HRESIMS and circular dichroism（CD）. Compound 2 exhibited a weak inhibitory ratio for bglucuronidase release induced by platelet-activating factor（PAF） in rat polymorphonuclear leukocytes（PMNs） in vitro.

关键词： Illicium activating elucidated spectroscopic hydroxymethyl circular shrub spectrometer Elmer extracts