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Preliminary study of the retention mechanism of planktonic copepods in the Jiulong Estuary in China

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Acta Oceanologica Sinica 2007年第4期26卷 156-163页

作者： SHANG Xu wang guizhong LI Shaojing GUO Donghui Department of Oceanography State Key laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China State Key Laboratory of Estuarine and Coastal Research East China Normal University Shanghai 200062 China

The horizontal and vertical distribution patterns of five planktonic copepods, Calanus sinicus, Acartia pacifica, Tortanus derjugini, Acartiella sinensis and Pseudodiaptomus poplesia, predominant in the Jiulong Estuary, were investigated from May 2003 to April 2004. The results showed that the distribution of these copepods was related to the tidal period but that each species had its own specific pattern. C. sinicus showed no tidal vertical migration behavior and was thought to be a non-resident species in this estuary. Among Acartia pacifica, T. derjugini,Acartiella sinensis, more individuals occurred in the surface than in the bottom waters during flood tide, and the pattern was reversed during ebb tide. The epibenthic copepod P. poplesia usually remained in the bottom waters in the upstream part of the estuary, but it displayed strong tidally-oriented vertical migration in the middle reaches of the estuary. Taking into account the hydrographic characteristics of the Jiulong Estuary, it was hypothesized that the planktonic copepods in this estuary had more or less adopted the mechanism of vertically migrating to the surface waters during flood tide in order to make use of the inflowing tide, and then sinking to the bottom during ebb tide to avoid being carried out of the estuary by net outflow.

关键词： estuary copepod retention mechanism tidal vertical migration

Response of antioxidant defense system in copepod Calanus sinicus Brodsky exposed to CO_2-acidified seawater

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Acta Oceanologica Sinica 2016年第8期35卷 82-88页

作者： ZHANG Dajuan GUO Donghui wang guizhong LI Shaojing Key Laboratory for Aquatic Ecology and Aquaculture of Tianjin College of Fisheries Tianjin Agricultural University College of Ocean and Earth Science Xiamen University

Marine zooplankton responds sensitively to elevated seawater CO_2 concentration. However, the underlying physiological mechanisms have not been studied well. We therefore investigated the effects of elevated CO_2concentration（0.08%, 0.20%, 0.50% and 1.00%） on antioxidant defense components, as well as two detoxification enzymes of Calanus sinicus（copepod）. The results showed that glutathione peroxidase（GPx） activity exposed to CO_2-acidified seawater was significantly stimulated while other antioxidant components, including glutathione-Stransferase（GST） activity, superoxide dismutase（SOD） activity decreased significantly with reduced glutathione（GSH） level and GSH/oxidized glutathione（GSSG） value. CO_2-acidified seawater exhibited stimulatory effects on adenosine triphosphatase（ATPase） activity and acetylcholinesterase（Ach E） activity was inhibited. Moreover, the results of principal component analysis indicated that 75.93% of the overall variance was explained by the first two principal components. The elevated CO_2 concentration may affect the metabolism and survivals of copepods through impacts these enzymes activities. Further studies are needed to focus on the synergistic effects of elevated CO_2 concentration and other environmental factors on copepods.

关键词： acidified seawater carbon dioxide Calanus sinicus antioxidant defense system

Genetic diversity and differentiation of three populations of Penaeus monodon Fabricus

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Acta Oceanologica Sinica 2008年第1期27卷 113-121页

作者： wang guizhong TAN Shuhua LI shaojing YE Haihui Department of Oceanography Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan （ Hainan Province of China, HN） and Malaysia （KD）, and the F1 generation of a Thailand broodstock population （CP） were examined by vertical polyacrylamide gel electrophoresis. Of 21 loci encoded by ten enzymes, 11 were polymorphic. The mean proportions of polymorphic loci of HN, KD and CP were 36. 36%, 45.45% and 50.00% , with the average heterozygosities of 0.135, 0.181 and 0.191, and the effective numbers of alleles per loci were 1. 300, 1. 330 and 1. 329, respectively. The divergent indexes of HN, KD and CP were0.023, 0.124, and 0.117, respectively. The genetic distance between the two wild populations was 0.005, and the gene differentiation coefficient was also very low （0.014）. The results indicated that the F1 generation population had a higher genetic diversity than the two wild populations, and that the HN population had the lowest one. There was no significant differentiation between HN and KD populations and all populations existed with a slight heterozygote excess.

关键词： Penaeus monodon isozyme genetic structure genetic differentiation

Effects of biogenic amines on the testicular development in mud crabs Scylla serrata

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Acta Oceanologica Sinica 2006年第2期25卷 119-124页

作者： YE Haihui HUANG Huiyang LI Shaojing wang guizhong LI Qifu Oceanographic Department State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China Laboratory of Cell Biology School of Life Science Xiamen University Xiamen 361005 China

The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the mature sections in testes, and the ratio of Type B cells in androgenic gland were taken as the quantitative indexes. The in vivo injections indicated that： 5-HT can significantly promote the testicular development and the secretion of the androgenic gland in S. serrata; DA can inhibit the testicular development, but no influence on the secretion of the androgenic gland was found; no significant difference was observed between the OA-injected group and the concurrent control group. In vitro incubations showed that： 5-HT can stimulate the secretion of the brain and the thoracic ganglia, thus accelerating that of the androgenic gland; however, neither OA nor DA showed any significant influence on the secretion of the brain and the thoracic ganglionic mass. As to the optic ganglia, the three biogenic amines hardly have any effect on its secretion. It is the first time to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans through vitro experiments. Results corroborate that 5-HT activates the brain and the thoracic ganglia to secret GSH first, then promote the testicular development through the activity of the androgenic gland.

关键词： biogenic amines Scylla serrata neuroendocrine organs androgenic gland reproduction

Proteomic approach for acute-phase proteins of hemolymph and muscles in Scylla serrata challenged by a pathogenic bacterium

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Frontiers in Biology 2006年第3期1卷 254-258页

作者： LIU Wenjie SU Jing wang guizhong wang Sanying Center of Proteomics Department of BiologySchool of Life SciencesXiamen UniversityXiamen 361005China

Acute-phase response is documented to be a significant mechanism of innate immunity in vertebrates and *** this study,proteomic methodologies were applied for different protein expressions in hemolymph of Scylla serrata challenged by Vibrio parahaemolyticus after immunization,and in muscles of the crabs separately chal-lenged by ***,*** and Aeromonas ***-regulated cryptocyanin is documented in the hemolymph and up-regulated calexcitin,wingless(fragment)and tachykinin-related peptide in the muscle as acute-phase *** the four altered proteins were responsible for bacterial stress,but cryptocyanin seemed to be a memory response protein against the chal-lenge by a live bacterium after immunization of the live *** up-regulated proteins can be indicative of an understanding of immunity of a crab.

关键词： Scylla serrata proteomics acute phase re-sponse acute phase protein hemolymph muscle differen-tial proteins

Nonparametric estimations of the sea state bias for a radar altimeter

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Acta Oceanologica Sinica 2017年第9期36卷 108-113页

作者： MIAO Hongli JING Yujie JIA Yongjun LIN Mingsen ZHANG Guoshou wang guizhong College of Information Science and Engineering Ocean University of China National Satellite Ocean Application Service State Oceanic Administration

To estimate the sea state bias（SSB） for radar altimeter, two nonparametric models, including a Nadaraya-Watson（NW） kernel estimator and a local linear regression（LLR） estimator, are studied based on the Jason-2 altimeter data. Selecting from different combinations of the Gaussian kernel function, spherical Epanechnikov kernel function, a fixed bandwidth and a local adjustable bandwidth, it is observed that the LLR method with the spherical Epanechnikov kernel function and the local adjustable bandwidth is the optimal nonparametric model for the SSB estimation. The comparisons between the nonparametric and parametric models are conducted and the results show that the nonparametric model performs relatively better at high-latitudes of the Northern Hemisphere. This method has been applied to the HY-2A altimeter as well and the same conclusion can be obtained.

关键词： radar altimeter sea state bias significant wave height wind speed nonparametric model parametric model

Optimization of Culture Conditions and Medium Composition for the Marine Algicidal Bacterium Alteromonas sp. DH46 by Uniform Design

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Journal of Ocean University of China 2013年第3期12卷 385-391页

作者： LIN Jing ZHENG Wei TIAN Yun wang guizhong ZHENG Tianling State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems School of Life SciencesXiamen University

Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic *** HAB control techniques have been developed,and biological methods have been paid more *** bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner,and kill or damage the algal cells.A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas *** culture conditions were optimized using a single-factor test *** including carbon source,nitrogen source,temperature,initial pH value,rotational speed and salinity were *** results showed that the cultivation of the bacteria at 28℃ and 180 r min-1with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain *** optimal medium composition for strain DH46 was determined by means of uniform design experimentation,and the most important components influencing the cell density were tryptone,yeast extract,soluble starch,NaNO3 and *** the following culture medium was used(tryptone 14.0g,yeast extract 1.63g,soluble starch 5.0 g,NaNO3 1.6 g,MgSO4 2.3 g in 1L),the largest bacterial dry weight(7.36 g L-1) was obtained,which was an enhancement of 107% compared to the initial medium;and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization.

关键词： algicidal bacteria optimization culture conditions medium composition uniform design

Immunocytological evidence for the presence of vertebrate FSH and LH-like substances in the brain and thoracic ganglion of the swimming crab,Portunus trituberculatus

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Progress in Natural Science:Materials International 2008年第11期18卷 1453-1457页

作者： Huiyang Huang Haihui Ye Shaojing Li guizhong wang Department of Oceanography,Xiamen University,Xiamen 361005,China Department of Oceanography Xiamen University Xiamen 361005 China

Investigating the distribution of follicle stimulating hormone(FSH)and luteinizing hormone(LH)in the swimming crab, Portunus trituberculatus,helps in the understanding of its reproductive regulation *** study has detected the location of vertebrate FSH-and LH-like substances in the brain and the thoracic ganglion mass with immunocytochemical *** immunoreaction is very weak or negative during the immature period and the post-spawning period,while it is stronger in the mature *** of FSH-like immunoreactive cells resembles that of LH-like *** immunoreactive cells were observed chiefly in the anterior median cell-cluster of the protocerebrum with stronger *** were only a few in the inboard cell-cluster of the olfactory lobe of the deutocerebrum and the posterior cell-cluster of the *** cells were located in all the three ganglia in the thoracic ganglion *** results indicated that substances resembling the vertebrate FSH and LH exist in ***,and their expression has some correlation with the developmental stage of ovaries,thus they might engage in the ovary development and ovulation.

关键词： Follicle stimulating hormone(FSH) Luteinizing hormone(LH) Portunus trituberculatus Brain Thoracic ganglion mass Immunocytochemistry

Immunorecognition of estrogen and androgen receptors in the brain and thoracic ganglion mass of mud crab,Scylla paramamosain

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Progress in Natural Science:Materials International 2008年第6期18卷 691-695页

作者： Haihui Ye*,Huiyang Huang,Shaojing Li,guizhong wang Department of Oceanography,Xiamen University,Xiamen 361005,China Department of Oceanography Xiamen University Xiamen 361005 China

The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone(GSH)which stimulates the maturation of *** the previous experiments,sex steroid hormones(estradiol,testosterone,progesterone,etc.)have been detected from the ***,the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so *** the present experiment,monoclonal antibodies were applied to investigate the immuno-recognition of estrogen receptor(ER)and androgen receptor(AR)in the brain and the thoracic ganglion mass of Scylla *** results showed that the distribution of the immunopositive substances of ER and AR was extremely *** distributed in the protocerebrum,deutocerebrum and tritocerebrum of the brain,and mainly in *** the thoracic ganglion mass,immuno-positive substances distributed in the subesophageal ganglion,thoracic ganglion and abdominal ganglion,and mostly in subesophageal *** substances of ER and AR mostly existed in the cytoplasm of *** present study will provide mor-phological evidence for the origin and the evolution of ER and *** addition,the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.

关键词： Scylla paramamosain Estrogen receptor Androgen receptor Brain Thoracic ganglion mass Immunocytochemistry