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Soluble expression of active human β-defensin-3 in Escherichia coli and its effects on the growth of host cells

Chinese Medical Journal 2007年第8期120卷 708-713页

作者： SI Li-gang LIU Xi-cheng LU You-yong wang gen-yu LI Wen-mei Beijing Pediatric Research Institute Beijing Children＇s Hospital Affiliated to Capital Medical University Beijing 100045 China Molecular Oncology Laboratory Beijing Institute for Cancer Research School of Oncology Peking University Beijing 100034 China

Background Human β-defensin-3 （HBD3） is an epithelial peptide that has been demonstrated to have a salt-insensitive broad spectrum of potent antimicrobial activity. Expressing antimicrobial peptides in Escherichia coil （E. colt） is very difficult for it can result in death of the bacterial host cells. Our aim was to establish a prokaryotic system expressing soluble HBD3 protein and demonstrate the antimicrobial activity of the expressed protein. We then studied whether the host cells would activate the suicide pathways. Methods We first cloned the complementary DNA coding for the mature chain of HBD3, inserted it into the vector PGEX-KG then transformed E. coil BL21 （DE3） with the appropriate recombinant plasmid. After induction with 0.5 mmol/L isopropyl-1-thio-β-D-galactopyranoside （IPTG） the transformed E. cofiproduced a recombinant glutathione S-transferase and HBD3 （GST-HBD3） fusion protein. The fusion protein was treated with thrombin to produce pure HBD3 protein then the antimicrobial activity of HBD3 was evaluated in a liquid microdilution assay. Results The fusion protein GST-HBD3 was efficiently cleaved by thrombin and yielded HBD3 that had anti-staphylococcus aureus activity with a minimal inhibitory concentration level of 12.5 μg/ml. The E. coil strain expressing the recombinant protein did not grow slower than the empty vector strain. Conclusion Active HBD3 in E. coil by expressing the recombinant protein GST-HBD3 could be produced, and suicide did not occur in the E. coli strain expressing the recombinant protein.

关键词： human beta-defensin-3 antimicrobial peptide prokaryotic expression

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Light People:Professor Manijeh Razeghi

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Light(Science & Applications) 2024年第8期13卷 1451-1462页

作者： Hui wang Cun yu Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China

The sense of light is the first sensation the human body *** importance of light is ***,we all know that the light we can see and perceive covers only a small section of the ***,for Light People,we feature a researcher who is committed to exploring different spectral bands of light ranging from deep ultraviolet to terahertz waves and working on quantum semiconductor technology,*** Razeghi of the Northwestern University in the United *** for her quick thinking and witty remarks,*** is passionate about life and always kind to *** a scientist,she does not limit her research to a single focus,instead,she works on the entire process from material selection,device design,processing,and manufacturing,all the way to product *** has a strong passion for education,a commitment unwavered by fame or *** her students,she is both a reliable source of knowledge and a motherly figure with a caring *** firmly believes that all things in nature can give her energy and *** science,she is a true“pioneer”in research and a“miner”of scientific *** advises young scientists to enjoy and love what they do,and turn their research into their *** a female scientist,she calls on all women to realize their true value and ***,let’s hear from Professor Manijeh Razeghi,a true star who radiates energy and light.

关键词： mother instead passion

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Light People:Professor Laura Na Liu

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Light(Science & Applications) 2024年第2期13卷 184-189页

作者： Hui wang Cun yu Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of Sciences130033ChangchunChina

Editorial The DNA nanotechnology outlines a new chapter in biological sciences,opening a new era of scientific and technological advancements.A pioneer of DNA nanotechnology,Professor Liu from the University of Stuttgart in Germany,our latest Light People,has been working at the interface,where nanophotonics meets biology and *** the path of scientific explorations.

关键词： chemistry. advancement pioneer

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Annihilator Ideals of Indecomposable Modules of Finite-Dimensional Pointed Hopf Algebras of Rank One

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Algebra Colloquium 2024年第1期31卷 149-164页

作者： yu wang School of Mathematics and Physics Jiangsu University of Technology ChangzhouJiangsu 213001China

Let H be a finite-dimensional pointed Hopf algebra of rank one over an algebraically closed feld of characteristic *** describe all annihilator ideals of indecomposable H-modules by *** particular,we give the classification of all ideals of the finite-dimensional pointed rank one Hopf algebra of nilpotent type over the Klein 4-group.

关键词： indecomposable module annihilator ideal primitive central orthogonal idem-potent element

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Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway

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World Journal of Gastroenterology 2024年第20期30卷 2709-2725页

作者： Kai-Di Chen Kui-Ling wang Chen Chen Yi-Jia Zhu Wen-Wen Tang yu-Ji wang Ze-Peng Chen Lin-Hai He yu-gen Chen Wei Zhang Jiangsu Province Hospital of Chinese Medicine The Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina The No.1 Clinical Medical College Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina Department of Colorectal Surgery Jiangsu Province Hospital of Chinese MedicineThe Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina Jiangsu Province Key Laboratory of Tumor Systems Biology and Chinese Medicine The Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina Jiangsu Collaborative Innovation Center of Chinese Medicine in Prevention and Treatment of Tumor The Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina

BACKGROUND Constipation,a highly prevalent functional gastrointestinal disorder,induces a significant burden on the quality of patients'life and is associated with substantial healthcare ***,identifying efficient therapeutic modalities for constipation is of paramount *** stress is a pivotal contributor to colonic dysmotility and is the underlying pathology responsible for constipation ***,we postulate that hydrogen therapy,an emerging and promising intervention,can serve as a safe and efficacious treatment for *** To determine whether hydrogen-rich water(HRW)alleviates constipation and its potential *** Constipation models were established by orally loperamide to Sprague-Dawley *** freely consumed HRW,and were recorded their 24 h total stool weight,fecal water content,and charcoal propulsion *** samples were subjected to 16S rDNA gene *** non-targeted metabolomic analysis,malondialdehyde,and superoxide dismutase levels were *** tissues were stained with hematoxylin and eosin,Alcian blue-periodic acid-Schiff,reactive oxygen species(ROS)immunofluorescence,and immunohistochemistry for cell growth factor receptor kit(c-kit),PGP 9.5,sirtuin1(SIRT1),nuclear factor-erythroid-2-related factor 2(Nrf2),and heme oxygenase-1(HO-1).Quantitative real-time PCR and western blot analysis were conducted to determine the expression level of SIRT1,Nrf2 and HO-1.A rescue experiment was conducted by intraperitoneally injecting the SIRT1 inhibitor,EX527,into constipated ***460 cells were induced with H2O2 and treated with the metabolites to evaluate ROS and SIRT1 *** HRW alleviated constipation symptoms by improving the total amount of stool over 24 h,fecal water content,charcoal propulsion rate,thickness of the intestinal mucus layer,c-kit expression,and the number of intestinal *** modulated intestinal microbiota imbalance and abnormaliti

关键词： Hydrogen-rich water Constipation Sirtuin1 Oxidative stress Gut microbiota Serum metabolites

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Possibilities for the synthesis of superheavy element Z=121 in fusion reactions

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Nuclear Science and Techniques 2024年第6期35卷 90-100页

作者： Ming-Hao Zhang yu-Hai Zhang Ying Zou Xiu-Xiu Yang gen Zhang Feng-Shou Zhang The Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China School of Physical Science and Technology Guangxi UniversityNanning 530004China National Laboratory of Heavy Ion Accelerator of Lanzhou Center of Theoretical Nuclear PhysicsLanzhou 730000China

Based on the dinuclear system model,the calculated evaporation residue cross sections matched well with the current experimental *** synthesis of superheavy elements Z=121 was systematically studied through combinations of stable projectiles with Z=21-30 and targets with half-lives exceeding 50 *** influence of mass asymmetry and isotopic dependence on the projectile and target nuclei was investigated in *** reactions^(254)Es(^(46)Ti,3n)^(297)121 and^(252)Es(^(46)Ti,3n)^(295)121 were found to be experimentally feasible for synthesizing superheavy element Z=121,with maximal evaporation residue cross sections of 6.619 and 4.123 fb at 219.9 and 223.9 MeV,respectively.

关键词： Superheavy nuclei Dinuclear system model Fusion reaction Evaporation residue cross section

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Recent progress in all-inorganic tin-based perovskite solar cells:a review

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Science China Chemistry 2024年第4期67卷 1117-1136页

作者： Minghao wang yu Shi School of Electronics and Information Engineering Wuxi UniversityWuxi 214105China Institute of Advanced Materials Nanjing Tech UniversityNanjing 211816China

Recently,power conversion efficiency(PCE)of organic-inorganic hybrid perovskite solar cells(PSCs)has been improved to26.1%.However,the toxicity issue of lead still hinders the large-scale commercial production and application of lead(Pb)-based halide ***,environmentally friendly tin(Sn)-based perovskites have recently shown excellent *** them,the all-inorganic Sn-based perovskites,including CsSnX_(3) and Cs_(2)SnX_(6),have better stability,more suitable bandgap,and higher charge mobility,which have attracted more attention from researchers than organic-inorganic Sn-based hybrid ***,we review recent research progress on all-inorganic Sn-based perovskite materials and corresponding solar cell ***,we also summarize the current challenges and future research directions for this type of PSCs.

关键词： solar cells perovskite all-inorganic CsSnX_(3) Cs_(2)SnX_(6)

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Steel-refractory reactions in lanthanum-,cerium-,and ytrium-added steels

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Journal of Iron and Steel Research International 2024年第6期31卷 1473-1485页

作者： Jian Kang Hong-po wang yu wang College of Materials Science and Engineering Chongqing UniversityChongqing 400044China The State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China

A systematic experimental study was conducted on the similarities and differences in the reactions between commonly used rare earth elements(REEs)and frequently used refractories in the steelmaking *** results indicate that the reaction behaviors of La,Ce,and Y with MgO,Al_(2)O_(3),and MgO·Al_(2)O_(3) crucibles differed *** consumption rates of La,Ce,and Y in the molten steel were in descending order of La>Ce>Y for smelting with all three kinds of *** the REEs reacted with the crucible,the contents of Mg and Al showed a trend of first increasing and then *** laboratory-scale smelting conditions,the reactions related to REEs were dominated by steel-crucible reactions,and the contact area between the unit mass of molten steel and refractory materials mainly determined the consumption rates of ***-and Ce-crucible reactions generated loose and porous reaction interfaces;however,Y-crucible reactions generated a dense Y_(2)O_(3) layer,dramatically suppressing the consumption rate of *** calculation indicated that the reactions between rare earth and different refractory materials were first-order *** crucible materials significantly affected the product type and morphology of the steel-crucible reaction interfaces,thereby influencing the consumption rate of *** reactions between REEs and refractories must be seriously considered,especially for small-scale smelting.

关键词： Rare earth Inclusion Refractory Thermodynamics Dynamics

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Numerical simulations of Atlantic meridional overturning circulation(AMOC)from OMIP experiments and its sensitivity to surface forcing

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Journal of Oceanology and Limnology 2024年第2期42卷 454-467页

作者： Xiaowei wang Yongqiang yu State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics(LASG) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China

Atlantic meridional overturning circulation(AMOC)plays an important role in transporting heat meridionally in the Earth’s climate system and is also a key metrical tool to verify oceanic general circulation *** OMIP(Ocean Model Intercomparison Project phase 1 and 2)simulations with LICOM3(version 3 of the LASG/IAP Climate System Ocean Model)developed at the State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics(LASG),Institute of Atmospheric Physics(IAP),are compared in this *** simulations well reproduce the fundamental characteristics of the AMOC,but the OMIP1 simulation shows a significantly stronger AMOC than the OMIP2 *** the LICOM3 configurations are identical between these two experiments,any differences between them must be attributed to the surface forcing *** analysis suggests that sea surface salinity(SSS)differences should be mainly responsible for the enhanced AMOC in the OMIP1 simulation,but sea surface temperature(SST)also play an unignorable role in modulating *** the North Atlantic,where deep convection occurs,the SSS in OMIP1 is more saline than that in *** find that in the major region of deep convection,the change of SSS has more significant effect on density than the change of *** a result,the SSS was more saline than that in OMIP2,leading to stronger deep convection and subsequently intensify the *** conduct a series of numerical experiments with LICOM3,and the results confirmed that the changes in SSS have more significant effect on the strength of AMOC than the changes in SST.

关键词： oceanic general circulation model(OGCM) Atlantic meridional overturning(AMOC) surface forcing deep convection

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Room-temperature Self-healing and Recyclable PDMS Elastomers with Superior Mechanical Properties for Triboelectric Nanogenerators

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Chinese Journal of Polymer Science 2024年第10期42卷 1566-1577,I0014页

作者： Shu-Juan wang Lu wang Hong-Zhe Su Zhi-Cheng Wu Qiao-gen Zhang Wei Fan Xin-Li Jing School of Chemistry Joint Innovation Center of Thermal Protection MaterialsXi’an Jiaotong UniversityXi’an710049China School of Electrical Engineering Xi’an Jiaotong UniversityXi’an710049China School of Textile Science and Engineering Xi’an Polytechnic UniversityXi’an710048China

Polydimethylsiloxane(PDMS)is an electron-withdrawing material that is widely used in triboelectric nanogenerators(TENGs).However,PDMS has poor mechanical properties after curing and is easily damaged when subjected to long-term ***,the long-term stable operation of TENGs under mechanical deformation cannot be *** this work,multiple hydrogen bonds and aromatic disulfide bonds were introduced into PDMS *** elastomers exhibited high toughness(a tensile strength of 1.91 MPa and an elongation at break of 340%),good recyclability,and room-temperature self-healing properties(healing efficiency of 96.4%in 24 h).Recyclable sandwich-like triboelectric nanogenerators with excellent electrical output performance(13.5 V)and room-temperature self-healing performance(24 h,98%recovery of self-generating performance)were prepared by utilizing the hydrogen bonding between the PDMS elastomer and *** work reported herein offers theoretical guidance and a compelling strategy for developing high-performance TENG negative friction layers.

关键词： Polydimethylsiloxane Aromatic disulfide bonds Hydrogen bonds Room temperature self-healing Triboelectric nanogenerator

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