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Assembly-free reads accurate identification(AFRAID)approach outperforms other methods of DNA barcoding in the walnut family(Juglandaceae)

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Plant Diversity 2025年第1期47卷 115-126页

作者： Yanlei Liu Kai Chen Lihu wang Xinqiang Yu Chao Xu Zhili Suo Shiliang Zhou Shuo Shi Wenpan dong School of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056038China State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Science Hebei Normal UniversityShijiazhuang 050024China School of Ecology and Nature Conservation Beijing Forestry UniversityBeijing 100083China

DNA barcoding has been extensively used for species ***,species identification of mixed samples or degraded DNA is limited by current DNA barcoding *** this study,we use plant species in Juglandaceae to evaluate an assembly-free reads accurate identification(AFRAID)method of species identification,a novel approach for precise species identification in ***,we determined(1)the accuracy of DNA barcoding approaches in delimiting species in Juglandaceae,(2)the minimum size of chloroplast dataset for species discrimination,and(3)minimum amount of next generation sequencing(NGS)data required for species *** found that species identification rates were highest when whole chloroplast genomes were used,followed by taxon-specific DNA barcodes,and then universal DNA *** identification of 100%was achieved when chloroplast genome sequence coverage reached 20%and the original sequencing data reached 500,000 *** accurately identified species for all samples tested after 500,000 clean reads,with far less computing time than common *** results provide a new approach to accurately identify species,overcoming limitations of traditional DNA *** method,which uses next generation sequencing to generate partial chloroplast genomes,reveals that DNA barcode regions are not necessarily fixed,accelerating the process of species identification.

关键词： DNA barcode Species identification Random DNA barcode Juglandaceae Assembly-free

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Behavior and outcomes of 141 acquired lacrimal sac mucoceles treated via endoscopic dacryocystorhinostomy

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International Journal of Ophthalmology(English edition) 2025年第2期18卷 244-250页

作者： Ying-Jie Ma Guang-Ming Zhou Zhi-Rong Liu Jin-fei Wei Xin-Yu Li Bin dong Lin-Juan Wu Wen-Can Wu Bo Yu National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhou 325027Zhejiang ProvinceChina The Eye Hospital School of Ophthalmology&OptometryWenzhou Medical UniversityWenzhou 325027Zhejiang ProvinceChina

AIM:To investigate the clinical profile of patients with acquired lacrimal sac mucocele(ALSM)and evaluate the efficacy of endoscopic dacryocystorhinostomy(En-DCR)for this ***:En-DCRs were performed on 141 patients with ALSM patients from January 2016 to March *** clinical baseline information and magnetic resonance imaging(MRI)images were recorded and *** assess the effectiveness of En-DCR therapy,both anatomical and functional success rate was assessed during a 12mo ***:A total of 141 patients,with a mean age of 57.70±14.11y,were enrolled in this *** of the patients were female(n=91;64.54%)and all had unilateral *** patients had a previous history of epiphora and purulent secretion,and the duration from lacrimal duct obstruction to mucocele formation ranged from 6 to *** findings consistently revealed an enlarged sac diameter,fluid accumulation separated by a thin rim from adjacent tissues,which is indicative of lacrimal sac ***-DCR was performed with an anatomical success rate of 93.62%and a functional success rate of 81.56%.CONCLUSION:ALSM is more commonly seen in females and *** is essentially a complication of lacrimal duct *** characteristics can be used for precise clinical diagnosis,while En-DCR emerges as an optimal therapy for this *** results provide a comprehensive reference for the diagnosis and treatment of ALSM.

关键词： acquired lacrimal sac mucocele clinical profile magnetic resonance imaging endoscopic dacryocystorhinostomy

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Novel high-temperature-resistant material SbLaO_(3) with superior hardness under high pressure

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Chinese Physics B 2025年第2期34卷 74-80页

作者： Haoqi Chen Haowen Jiang Xuehui Jiang Jialin wang Chengyao Zhang Defang Duan Jing dong Yanbin Ma School of Science Harbin University of Science and TechnologyHarbin 150080China College of Physics Jilin UniversityChangchun 130012China School of Public Health Mudanjiang Medical UniversityMudanjiang 157011China

Perovskites have garnered significant attention in recent years. However, the presence of La atoms at the B-site in ABX_(3) structures has not yet been observed. Under high pressure, perovskites exhibit unexpected phase transitions. In this study, we report the discovery of SbLaO_(3) under ambient pressure, with a space group of R3m. Mechanical property calculations indicate that it is a brittle material, and it possesses a band gap of 4.0266 e V, classifying it as an *** also investigate the phase at 300 GPa, where the space group shifts to P2_(1/m). Additionally, the P2_(1/m) phase of LaInO_(3) under 300 GPa is explored. Ab initio molecular dynamics calculations reveal that the melting point of SbLaO_(3) is exceptionally high. The inclusion of Sb alters the electronic structure compared with LaInO_(3), and the Vickers hardness(H_(v)) is estimated to reach 20.97 GPa. This research provides insights into the phase transitions of perovskites under high pressure.

关键词： high pressure molecular dynamics phase transition

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基于代理模型的大尺寸复合材料结构静强度可靠性及灵敏度分析

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复合材料学报 2025年第1期42卷 570-578页

作者：张佳锦王栋上海飞机制造有限公司复合材料中心上海201324 中国商飞北京民用飞机技术研究中心北京102211

基于代理模型的结构可靠性分析方法目前已有多种代理模型和多种失效概率计算方法,参数灵敏度分析也存在多种灵敏度指标,为探讨大尺寸复合材料结构可靠性分析方法,并研究不同代理模型和计算方法对民机复合材料结构静强度预测结果的差异性... 详细信息

基于代理模型的结构可靠性分析方法目前已有多种代理模型和多种失效概率计算方法,参数灵敏度分析也存在多种灵敏度指标,为探讨大尺寸复合材料结构可靠性分析方法,并研究不同代理模型和计算方法对民机复合材料结构静强度预测结果的差异性,以某型飞机碳纤维复合材料升降舵结构为研究对象,考虑单层板力学性能、厚度、密度和不同部位铺层角度等17种输入变量的不确定性,利用Matlab和Optistruct仿真软件,构建Kriging、PC-Kriging和支持向量回归机(SVR)3种代理模型,结合蒙特卡洛法(MCS)和Subset两种方法求解结构失效概率,根据代理模型验证误差选取最准确的代理模型计算Morris基本效应筛选指标和Sobol指标,从而获得关键设计参数排序,为民机复合材料结构可靠性设计提供参考。

关键词：复合材料结构代理模型 Kriging模型可靠性分析灵敏度分析

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Daratumumab improves the hematological response and kidney function of patients with light chain deposition disease

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中华医学杂志(英文版) 2025年第2期138卷 2024 Nov 13页

作者： Zhang Xin Yu Xiaojuan wang Suxia dong Yujun Zhao Minghui Zhou Fude Renal Division Department of Medicine Peking University First Hospital Beijing China Institute of Nephrology Peking University Beijing China Key Laboratory of Renal Disease Ministry of Health of China Beijing China Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases Chinese Academy of Medical Sciences Beijing China Laboratory of Electron Microscopy Pathological Centre Peking University First Hospital Beijing China Hematological Division Department of Medicine Peking University First Hospital Beijing China Peking-Tsinghua Center for Life Science Academy for Advanced Interdisciplinary Studies Peking University Beijing China

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Computed tomography enterography-based radiomics for assessing mucosal healing in patients with small bowel Crohn's disease

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World Journal of Gastroenterology 2025年第3期31卷 62-72页

作者： Hao Ding Yuan-Yuan Fang Wen-Jie Fan Chen-Yu Zhang Shao-fei wang Jing Hu Wei Han Qiao Mei Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Radiation Oncology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Gastroenterology Suzhou First People's HospitalSuzhou 234099Anhui ProvinceChina

BACKGROUND Mucosal healing(MH)is the major therapeutic target for Crohn's disease(CD).As the most commonly involved intestinal segment,small bowel(SB)assessment is crucial for CD ***,it poses a significant challenge due to its limited accessibility through conventional endoscopic *** To establish a noninvasive radiomic model based on computed tomography enterography(CTE)for MH assessment in SBCD *** Seventy-three patients diagnosed with SBCD were included and divided into a training cohort(n=55)and a test cohort(n=18).Radiomic features were obtained from CTE images to establish a radiomic *** demographics were analysed to establish a clinical model.A radiomic-clinical nomogram was constructed by combining significant clinical and radiomic *** diagnostic efficacy and clinical benefit were evaluated via receiver operating characteristic(ROC)curve analysis and decision curve analysis(DCA),*** Of the 73 patients enrolled,25 patients achieved *** radiomic-clinical nomogram had an area under the ROC curve of 0.961(95%confidence interval:0.886-1.000)in the training cohort and 0.958(0.877-1.000)in the test cohort and provided superior clinical benefit to either the clinical or radiomic models alone,as demonstrated by *** These results indicate that the CTE-based radiomic-clinical nomogram is a promising imaging biomarker for MH and serves as a potential noninvasive alternative to enteroscopy for MH assessment in SBCD patients.

关键词： Crohn’s disease Computed tomography enterography Mucosal healing Nomogram Radiomics

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我国生态工业园建设中存在的问题与对策

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石家庄经济学院学报 2009年第2期32卷 41-44页

作者：董王飞杨耀富西北大学经济管理学院陕西西安710127

我国生态工业园的建设主要存在两方面的问题:一是污染控制不力,二是生态产业链建设并不成功。导致第一个问题的原因是在生态工业园建设过程中目标分工不明确,导致第二个问题的原因是生态产业链的经济效率低下。文章对这些问题进行了剖析... 详细信息

我国生态工业园的建设主要存在两方面的问题:一是污染控制不力,二是生态产业链建设并不成功。导致第一个问题的原因是在生态工业园建设过程中目标分工不明确,导致第二个问题的原因是生态产业链的经济效率低下。文章对这些问题进行了剖析,并提出了对策和建议。

关键词：生态工业园生态产业链问题对策

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Emerging patterns of antimicrobial resistance:Concurrent presence of mcr-1.1 and mcr-8.1 in a ST15 Klebsiella pneumoniae isolated from poultry in China

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Journal of Integrative Agriculture 2025年第1期24卷 403-407页

作者： dong'an Cui Panpan Liu Ling wang Jiongjie He Yuzhang Yan Mengke Ru Baocheng Hao Yan Sun Shengyi wang Key Laboratory of New Animal Drug Project Gansu Province/Key Laboratory of Veterinary Pharmaceutical DevelopmentMinistry of Agriculture and Rural AffairsLanzhou Institute of Husbandry and Pharmaceutical SciencesChinese Academy of Agricultural SciencesLanzhou 730050China College of Animal Science and Technology Guangxi UniversityNanning 530005China China Institute of Veterinary Drug Control Beijing 100081China

Colistin serves as a crucial treatment for multidrugresistant(MDR)Gram-negative bacterial ***,its excessive use has contributed to an increase in colistin-resistant strains within livestock production(Binsker et al.2022).The mobile colistin resistance gene,mcr,which reduces colistin affinity by incorporating phosphoethanolamine(pEtN)into the bacterial lipopolysaccharide,significantly compromises the effectiveness of colistin treatments(Liu et al.2016).

关键词： poultry treatment compromise

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Unilamellar MnO_(2)nanosheets confined Ru-clusters combined with pulse electrocatalysis for biomass electrooxidation in neutral electrolytes

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Science Bulletin 2025年第2期70卷 193-202页

作者： Yuanqing He Chongyang Ma Shiheng Mo Chung-Li dong Wei Chen Shuo Chen Huan Pang Renzhi Ma Shuangyin wang Yuqin Zou School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225009China State Key Laboratory of Chem/Bio-Sensing and Chemometrics Advanced Catalytic Engineering Research Center of the Ministry of EducationCollege of Chemistry and Chemical EngineeringThe National Supercomputer Centers in ChangshaHunan UniversityChangsha 410082China Department of Physics Tamkang UniversityNew Taipei City 25137China School of Materials Science and Engineering Central South UniversityChangsha 410083China International Research Center for Materials Nanoarchitectonics(WPI-MANA) National Institute for Materials Science(NIMS)TsukubaIbaraki 305-0044Japan

The electrochemical oxidation of 5-hydroxymethylfurfural(HMFOR)in alkaline electrolyte is a promising strategy for producing high-value chemicals from biomass ***,the disproportionation of aldehyde groups under strong alkaline conditions and the polymerization of HMF to form humic substances can impact the purity of 2,5-furandicarboxylic acid(FDCA)*** use of neutral electrolytes offers an alternative environment for electrolysis,but the lack of OH^(-)ions in the electrolyte often leads to low current density and low yields of *** this study,a sandwich-structured catalyst,consisting of Ru clusters confined between unilamellar MnO_(2)nanosheets(S-Ru/MnO_(2)),was used in conjunction with an electrochemical pulse method to realize the electrochemical conversion of5-hydroxymethylfurfural into FDCA in neutral *** electrolysis and the strong electron transfer between Ru clusters and MnO_(2)nanosheets help maintain Ru in a low oxidation state,ensuring high *** increased*OH generation led to a groundbreaking current density of 47 mA/cm^(2)at1.55 V *** hydrogen electrode(RHE)and an outstanding yield rate of 98.7%for FDCA in a neutral *** work provides a strategy that combines electrocatalyst design with an electrolysis technique to achieve remarkable performance in neutral HMFOR.?2024 Science China *** by Elsevier *** Science China *** rights are reserved,including those for text and data mining,AI training,and similar technologies.

关键词： Electrocatalytic 5-hydroxymethylfurfural oxidation Neutral electrolyte Electrocatalysts Nano-confined structure Electrochemical pulse

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Lung metastasis following temporary discontinuation of lenvatinib and tislelizumab in hepatocellular carcinoma:A case report

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World Journal of Gastrointestinal Surgery 2025年第3期17卷 391-397页

作者： Chen-dong wang Run-dong Liu Ming-Jie Liu Jia Song Hepatic Surgery Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Oncology Liyuan HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430062Hubei ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is a prevalent malignancy in China,primarily diagnosed at advanced stages,which limits treatment options and increases mortality *** therapy,which includes systemic and locoregional treatments,aims to render unresectable tumors ***,research is scant on the risks of disease progression during the temporary cessation of targeted drugs and immune checkpoint inhibitors before *** SUMMARY This report describes a 58-year-old male with HCC who developed lung meta-stases following the discontinuation of lenvatinib and tislelizumab,revealing the necessity for further investigation into the management of HCC patients during the perioperative period,particularly concerning the timing and duration of tar-geted therapy and *** Our study highlights the complex challenges in managing advanced HCC and emphasizes the critical need for ongoing research to refine treatment strategies and improve patient outcomes.

关键词： Hepatocellular carcinoma Hepatic arterial infusion chemotherapy Lenvatinib Tislelizumab Conversion therapy Case report

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