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Chemical Research in Chinese Universities 2001年第1期17卷 102-107页

作者： wang chun ming ZHU Li College of Chemistry and Chemical Engineering Lanzhou UniversityLanzhou 730000P.R.China

The cyclic voltammetry(CV) and the square wave technique were used for the investigations of thallium(Ⅰ) underpotential deposition(UPD) on the silver electrode. A solution of 10 \{mmol/L\} HClO 4+10 mmol/L NaCl was selected as the supporting electrolyte. The calibration plots for Tl(Ⅰ) concentration in the range of 2×10 -9 -1×10 -7 mol/L were obtained. The detection limit was 5×10 -10 mol/L. For the solutions of 4 0×10 -9 mol/L thallium added before the urine sample pretreatment procedure, the average recovery was 105 6% with a relative standard deviation(RSD) of 15 5%.

关键词： Thallium(Ⅰ) Underpotential deposition Silver rotating disk electrode Cyclic voltammetry Square wave anodic stripping voltammetry

Electrochemical Study and Application on Shikonin at Poly（diallyldimethylammonium chloride） Functionalized Graphene Sheets Modified Glass Carbon Electrode

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Chemical Research in Chinese Universities 2013年第4期29卷 798-805页

作者： AN Jing LI Ji-ping CHEN Wen-xia YANG chun-xia HU Fang-di wang chun-ming School of Pharmacy Hospital of Lanzhou University College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 P. R. China

The electrochemical behaviors of shikonin at a poly（diallyldimethylammonium chloride） functionalized graphene sheets modified glass carbon electrode（PDDA-GS/GCE） have been investigated. Shikonin could exhibit a pair of well-defined redox peaks at the PDDA-GS/GCE located at 0.681 V（Epa） and 0.662 V（Epc）[vs. saturated calo- mel electrode（SCE）] in 0.1 mol/L phosphate buffer solution（pH=2.0） with a peak-to-peak separation of about 20 mV, revealing a fast electron-transfer process. Moreover, the current response was remarkably increased at PDDA- GS/GCE compared with that at the bare GCE. The electrochemical behaviors of shikonin at the modified electrode were investigated. And the results indicate that the reaction involves the transfer of two electrons, accompanied by two protons and the electrochemical process is a diffusional-controlled electrode process. The electrochemical para- meters of shikonin at the modified electrode, the electron-transfer coefficient（a）, the electron-transfer number（n） and the electrode reaction rate constant（ks） were calculated to be as 0.53, 2.18 and 3.6 s^-1, respectively. Under the optimal conditions, the peak current of differential pulse voltammetry（DPV） increased linearly with the shikonin concentra- tion in a range from 9A72×10^-8 mol/L to 3,789×10^-6 mol/L with a detection limit of 3,157× 10^-8 mol/L. The linear regression equation was Ip=O.7366c＋0.7855（R=0.9978; lp： 10-7 A, c： 10-8 mol/L）. In addition, the modified glass carbon electrode also exhibited good stability, selectivity and acceptable reproducibility that could be used for the sensitive, simple and rapid determination of shikonin in real samples. Therefore, the present work offers a new way to broaden the analytical application of graphene in pharmaceutical analysis.

关键词： Shikonin Poly(diallyldimethylammonium chloride) functionalized graphene sheets modified glass carbon electrode(PDDA-GS/GCE) Cyclic voltammetry Differential pulse voltammetry Electrochemical sensor

Isolating the Mutator Transposable Element Insertional Mutant Gene mio16 of Maize Using Double SelectedAmplification of Insertion Flanking Fragments (DSAIFF)

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Journal of Integrative Agriculture 2012年第10期11卷 1592-1600页

作者： ZHONG Wen-juan ZHANGMei-dong YANG Liu-qi wang ming-chun ZHENG Yong-lian YANG Wen-peng GAO You-jun National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University Institute of Upland Food Crops Guizhou Academy of Agricultural Sciences/Guizhou Center of Maize Engineering Techniques

Mutator transposable element （Mu） has been used as an effective tool to clone maize （Zea mays L.） genes. One opaque endosperm mutant （miol6） was identified in a pool of Mu inserted mutants. A modified method, termed the double selected amplification of insertion flanking fragments （DSAIFF）, was employed to isolate the Mu flanking fragments （MFFs） of miol6. The target site duplications （TSDs） isolated from the Msp I and Mse I digested MFFs had a same 9-bp sequence and were confirmed to be the flanking sequence of one identically inserted gene. Co-segregation analysis suggested that the MFFs were associated with the mutant opaque endosperm, and miol6 was mapped in silico onto the physical position ranged from 229 965 021 to 229 965 409 bp of the maize chromosome 4.09 bin. The full-length cDNA of the wild-type gene was obtained by an RT-PCR primer-scanning technique, and Mio16 was found to putatively encode a homolog of the Arabidopsis MAP3K delta-1 protein kinase. RT-PCR result the mRNA expression of miol6 region anchored by primers Mu20 and af276 was not interrupted by Mu insertion. Further researches will be done to elucidate how the expression of miol6 is alternated by Mu insertion.

关键词： maize (Zea mays L.) Mutator (Mu) transposable element Mu flanking fragments (MFFs) double selected amplification of insertion flanking fragments (DSAIFF) miol6

基于粗集与遗传算法相结合的文本模糊聚类方法

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电子与信息学报 2005年第4期27卷 548-551页

作者：王明春王正欧天津大学系统工程研究所

该文将粗集与遗传算法相结合的方法成功应用于文本模糊聚类。在聚类过程中,将权重参数的设定也通过编码由遗传算法确定,从而使得权重参数的设定具有科学性和可操作性,避免了在类似算法中确定权重时的主观性和不可靠性。最后的实例说... 详细信息

该文将粗集与遗传算法相结合的方法成功应用于文本模糊聚类。在聚类过程中,将权重参数的设定也通过编码由遗传算法确定,从而使得权重参数的设定具有科学性和可操作性,避免了在类似算法中确定权重时的主观性和不可靠性。最后的实例说明了算法的可行性。

关键词：粗集遗传算法文本挖掘模糊聚类

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果蝇杂种劣育与P因子转座调控

昆虫学报 2023年第7期66卷 959-968页

作者：王春明兰州大学生命科学学院兰州730000

果蝇Drosophila杂种劣育(hybrid dysgenesis)指某些品系果蝇间杂交,子代出现诸如卵巢发育不全、分离比异常、雄性个体减数分裂出现重组、高突变率、高频率染色体畸变甚至不育等异常现象。该现象可由多种转座因子(transposable element,... 详细信息

果蝇Drosophila杂种劣育(hybrid dysgenesis)指某些品系果蝇间杂交,子代出现诸如卵巢发育不全、分离比异常、雄性个体减数分裂出现重组、高突变率、高频率染色体畸变甚至不育等异常现象。该现象可由多种转座因子(transposable element,TE)频繁转座引起,包括:P因子、I因子、hobo因子、Penelope因子、Paris因子和Helena因子等。其中P因子是首个确定DNA序列的真核生物转座子,也是研究得最为充分的动物TE之一,已被开发成基因工程工具,在果蝇转基因研究中发挥重要作用。基因组中携带P因子的果蝇为父系品系(paternal strain),即P品系,无P因子的果蝇为母系品系(maternal strain),即M品系。M雌×P雄杂交子代,在繁殖过程中出现杂种劣育现象,而P雌×M雄、M雌×M雄以及P雌×P雄交配组合的后代都是正常的。近20年来,P因子调控机制研究取得重大进展,在原有阻遏蛋白机制外,又发现了与PIWI蛋白相互作用的RNA[P-element-induced wimpy testis(PIWI)-interacting RNA,piRNA]机制。阻遏蛋白机制基于P因子转座酶4个外显子间的3个内含子转录后剪切方式:如果3个内含子均被剪切,则产生的mRNA包含4个外显子,翻译为87 kD的转座酶,促进P因子转座;如果仅前2个内含子被剪切,则产生的mRNA在第3个内含子区段所含终止密码子处提前终止翻译,产生66 kD的阻遏蛋白,阻止P因子转座。近年发现的piRNA机制是更为普遍的TE调控机制,该机制类似细菌中发现的CRISPR来源的RNA(CRISPR-derived RNA,crRNA)机制,编码piRNA的基因也成簇排列,称为piRNA基因簇。piRNA基因簇转录出单链RNA前体分子,剪切为23~32 nt的piRNA,随即与PIWI蛋白结合形成复合体,并通过两个途径发挥作用:一是降解与piRNA序列互补的靶mRNA,发挥转录后沉默作用;另一是进入细胞核,指导P因子或piRNA基因簇序列的表观遗传修饰,发挥对P因子的转录抑制作用,和对piRNA基因簇的转录激活作用。研究发现,阻遏蛋白机制和piRNA机制在P因子转座调控中均发挥作用。本文可为果蝇杂种劣育现象的教学和科研工作提供帮助。

关键词：果蝇杂种劣育转座因子转座子 P因子 piRNA 阻遏蛋白

Aluminum（Ⅲ）ion sensor based on the sensitive membrane containing ***2O3 powders

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Journal of Chemistry and Chemical Engineering 2008年第4期2卷 47-52页

作者： MA Chuan-li HE Li-jun, TIAN Li-fang wang chun-ming （School of Chemistry. and Chemical Engineering Lanzhou University Lanzhou 730000 China

An AI^3＋ sensor based on the membrane of acetyl cellulose containing nano γ-Al2O3 crystals was studied. In the buffer solution of 0.5 mol/L CH3COOH-CH3COONa （pH=5.0）, the sensor responds to AI^3＋ in a linear range from 1.00×10^-5 to 1.00x10-^2 mol/L. A near-Nernstian response was obtained and the regression equation was E （mv） = -161.4-26.54 Ig [AI^3＋] with a detection limit of 7.90x10^-6 mol/L. More than 14 different ions as the considered interferences were tested and the relevant selectivity coefficients were determined using the separate solution method （SSM）. The sensor possesses many advantages including short conditioning time, fast response, and, especially, very good selectivity over a wide variety of other co-existing ions. The sample analysis on the aluminium migration amount from aluminium utensils to the solution was determined by this sensor. The analytical results were agreed with that of inductively coupled plasma-atomic emission spectroscopy（ICP-RES）.

关键词： sensor aluminum (Ⅲ) acetyl cellulose membrane nano γ-Al2O3

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现代企业如何建设团队绩效文化

科技管理研究 2007年第11期27卷 200-202页

作者：崔明王睿王春明兰州大学管理学院甘肃兰州730000

团队绩效文化是企业文化研究的新课题,是企业持续发展的不竭动力。创建团队绩效文化是企业文化建设的总趋势。本文分别从基础层、制度层和宣传层介绍了企业团队绩效文化建设的途径,并为其建设提供了基本方向。

关键词：团队绩效文化团队建设绩效考评

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新技术方法在新药研发中的应用

中国新药杂志 2024年第14期33卷 1458-1465页

作者：王春明牛张明张龙德睿智药(苏州)新药研发有限公司苏州215100 杭州德睿智药科技有限公司杭州310000

新药研发是一个漫长且复杂的过程,需要高投入并伴有高风险和较低的成功率,因此提高新药研发效率,以最快的速度获得高质量的药物分子是所有新药研发企业梦寐以求的结果。为了克服传统二维细胞和动物实验模型简单、与人相关性差、低预测性... 详细信息

新药研发是一个漫长且复杂的过程,需要高投入并伴有高风险和较低的成功率,因此提高新药研发效率,以最快的速度获得高质量的药物分子是所有新药研发企业梦寐以求的结果。为了克服传统二维细胞和动物实验模型简单、与人相关性差、低预测性和/或高成本的弊端,开发和使用人体相关性更好、预测性更高、耗时更少、花费更低、动物福利更好的新技术方法已经迫在眉睫。高通量组学、类器官、器官芯片、模型引导的药物开发、人工智能等新型的技术和方法经过几十年的发展已经越来越成熟,并已经在新药研发领域展现出潜在的应用价值和应用前景。本文将对上述5种新技术方法在新药研发中的应用进行综述,使读者能够对这些新技术方法有一定的了解,以促进这些新技术方法在新药研发中的应用。

关键词：新药研发新技术方法组学类器官器官芯片模型引导的药物开发人工智能

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通钢120t转炉实现计算机自动炼钢的实践

钢铁 2007年第5期42卷 82-86页

作者：关春立王明春刚占库通化钢铁股份有限公司第三炼钢厂吉林通化134003 达涅利公司北京代表处北京100004

用实际生产数据说明,通钢120 t转炉实现副枪和动、静态模型控制下的自动炼钢工艺后,吹炼过程稳定,补吹炉数少,钢铁料、合金消耗低,炉龄提高,转炉冶炼周期缩短,工作环境改善,使转炉副枪系统的应用取得了很好的经济效益和社会效益。总结... 详细信息

用实际生产数据说明,通钢120 t转炉实现副枪和动、静态模型控制下的自动炼钢工艺后,吹炼过程稳定,补吹炉数少,钢铁料、合金消耗低,炉龄提高,转炉冶炼周期缩短,工作环境改善,使转炉副枪系统的应用取得了很好的经济效益和社会效益。总结了在实现转炉自动炼钢工艺的过程中所做的主要工作和存在的问题,并确定了下一步工作的努力方向。

关键词：转炉自动炼钢实践

布地奈德联合万托林雾化吸入治疗小儿支气管哮喘的临床疗效

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中国妇幼保健 2012年第23期27卷 3668-3670页

作者：王明春江苏省徐州市鼓楼区妇幼保健所 221000

目的:探讨布地奈德联合万托林雾化吸入治疗小儿支气管哮喘的临床疗效和安全性。方法:70例支气管哮喘患儿随机分为治疗组和对照组。对照组给予常规治疗,治疗组在常规治疗的基础上,给予布地奈德联合万托林雾化吸入治疗,连续治疗3天。观察... 详细信息

目的:探讨布地奈德联合万托林雾化吸入治疗小儿支气管哮喘的临床疗效和安全性。方法:70例支气管哮喘患儿随机分为治疗组和对照组。对照组给予常规治疗,治疗组在常规治疗的基础上,给予布地奈德联合万托林雾化吸入治疗,连续治疗3天。观察治疗前后两组主要临床症状和体征改善情况,评价临床疗效,观察不良反应。结果:治疗组临床疗效总有效率为94.3%,显著高于对照组(71.4%),相比较有统计学差异(χ2=6.437,P<0.05)。治疗组喘憋消失、咳嗽缓解、肺部湿啰音和肺部哮鸣音消失时间明显低于对照组,两组相比较有统计学差异(t=5.898、4.899、4.982、7.420,P<0.01)。两组均无不良反应发生。结论:布地奈德联合万托林雾化吸入治疗小儿支气管哮喘是一种安全、有效的治疗方法,临床值得推广应用。

关键词：支气管哮喘布地奈德万托林雾化吸入