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OpNAC1 transcription factor regulates the biosynthesis of the anticancer drug camptothecin by targeting loganic acid O-methyltransferase in Ophiorrhiza pumila

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Journal of Integrative Plant Biology 2023年第1期65卷 133-149页

作者： Xiaolong Hao can wang Wei Zhou Qingyan Ruan Chenhong Xie Yinkai Yang Chengyu Xiao Yan Cai Jingyi wang Yao wang Xuebin Zhang Itay Maoz Guoyin Kai Laboratory of Medicinal Plant Biotechnology School of Pharmaceutical SciencesAcademy of Chinese Medical Science Zhejiang Chinese Medical UniversityHangzhou 310053 China State Key Laboratory of Crop Stress Adaptation and Improvement Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life Sciences Henan UniversityKaifeng 475001 China Department of Postharvest Science ARO The Volcani Center HaMaccabim Rd 68POB 15159Rishon LeZion 7528809 Israel

Camptothecin(CPT) is an anticancer pentacyclic quinoline alkaloid widely used to treat cancer patients worldwide. However, the biosynthetic pathway and transcriptional regulation of camptothecin are largely unknown. Ophiorrhiza pumila, the herbaceous plant from the Rubiaceae family, has emerged as a model plant for studying camptothecin biosynthesis and regulation. In this study, a high-quality reference genome of O. pumila with estimated size of ~456.90Mb was reported, and the accumulation level of camptothecin in roots was higher than that in stems and leaves. Based on its spatial distribution in the plant, we examined gene functions and expression by combining genomics with transcriptomic *** loganic acid O-methyltransferase(OpLAMTs)were identified in strictosidine-producing plant ***, and enzyme catalysis assays showed that OpLAMT1 and not OpLAMT2 could convert loganic acid into loganin. Further knock-out of OpL AMT1expression led to the elimination of loganin and camptothecin accumulation in O. pumila hairy *** key residues were identified in OpLAMT1 protein crucial for the catalytic activity of loganic acid to loganin. By co-expression network, we identified a NAC transcription factor, OpNAC1, as a candidate gene for regulating camptothecin *** hairy roots and biochemical assays demonstrated that OpNAC1 suppressed OpLAMT1 expression. Here, we reported on two camptothecin metabolic engineering strategies paving the road for industrial-scale production of camptothecin in CPT-producing plants.

关键词： camptothecin biosynthesis metabolic engineering strategies Ophiorrhiza pumila plant secondary metabolism transcriptional regulation

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A systematic review of the inequality of health burdens related to climate change

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Frontiers of Environmental Science & Engineering 2024年第5期18卷 137-153页

作者： Xinke Song Shihui Zhang Hai Huang Qun Ding Fang Guo Yaxin Zhang Jin Li Mingyu Li Wenjia Cai can wang State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing100084China Department of Earth System Science Tsinghua UniversityBeijing100084China

Climate change significantly impacts human health,exacerbating existing health inequalities and creating new *** study addresses the lack of systematic review in this area by analyzing 2440 publications,focusing on four key terms:health,disparities,environmental factors,and climate *** inclusion criteria limited the selection to English-language,peer-reviewed articles related to climate health hazards,ensuring the relevance and rigor of the synthesized *** process synthesized 65 relevant *** investigation revealed that recent research,predominantly from developed countries,has broadened its scope beyond temperature-related impacts to encompass diverse climate hazards,including droughts,extreme weather,floods,mental health issues,and the intersecting effects of Coronavirus Disease *** has highlighted exposure as the most studied element in the causal chain of climate change-related health inequalities,followed by adaptive capability and inherent *** most significant vulnerabilities were observed among populations with low socioeconomic status,ethnic minorities,and *** study further reveals research biases and methodological limitations,such as the paucity of attention to underdeveloped regions,a narrow focus on non-temperature-related hazards,challenges in attributing climate change effects,and a deficit of large-scale empirical *** findings call for more innovative research approaches and a holistic integration of physical,socio-political,and economic dimensions to enrich climate-health discourse and inform equitable policy-making.

关键词： Climate change Health impact Human well-being Inequality Vulnerability

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Effects of BaTiO3 and SrTiO3 as the buffer layers of epitaxial BiFeO3 thin films

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Science China(Physics,Mechanics & Astronomy) 2017年第6期60卷 69-73页

作者： Yu Feng can wang ShiLu Tian Yong Zhou Chen Ge HaiZhong Guo Meng He KuiJuan Jin GuoZhen Yang Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China Collaborative Innovation Center of Quantum Matter Beijing 100190 China

BiFeO_3 (BFO) thin films with BaTiO_3 (BTO) or SrTiO_3 (STO) as buffer layer were epitaxially grown on SrRuO_3-covered SrTiO_3 substrates. X-ray diffraction measurements show that the BTO buffer causes tensile strain in the BFO films, whereas the STO buffer causes compressive strain. Different ferroelectric domain structures caused by these two strain statuses are revealed by piezoelectric force microscopy. Electrical and magnetical measurements show that the tensile-strained BFO/BTO samples have reduced leakage current and large ferroelectric polarization and magnetization, compared with compressively strained BFO/STO. These results demonstrate that the electrical and magnetical properties of BFO thin films can be artificially modified by using a buffer layer.

关键词： BFO buffer layer strain

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Interpreting the vulnerability of power systems in cascading failures using multi-graph convolutional networks

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Frontiers of Information Technology & Electronic Engineering 2022年第12期23卷 1848-1861页

作者： Supaporn LONAPALAWONG Changsheng CHEN can wang Wei CHEN State Key Lab of CAD&CG Zhejiang UniversityHangzhou 310058China China Electric Power Research Institute Beijing 100192China College of Computer Science and Technology Zhejiang UniversityHangzhou 310058China

Analyzing the vulnerability of power systems in cascading failures is generally regarded as a challenging problem. Although existing studies can extract some critical rules, they fail to capture the complex subtleties under different operational conditions. In recent years, several deep learning methods have been applied to address this issue. However, most of the existing deep learning methods consider only the grid topology of a power system in terms of topological connections, but do not encompass a power system’s spatial information such as the electrical distance to increase the accuracy in the process of graph convolution. In this paper, we construct a novel power-weighted line graph that uses power system topology and spatial information to optimize the edge weight assignment of the line graph. Then we propose a multi-graph convolutional network(MGCN) based on a graph classification task, which preserves a power system’s spatial correlations and captures the relationships among physical components. Our model can better handle the problem with power systems that have parallel lines, where our method can maintain desirable accuracy in modeling systems with these extra topology features. To increase the interpretability of the model, we present the MGCN using layer-wise relevance propagation and quantify the contributing factors of model classification.

关键词： Power systems Vulnerability Cascading failures Multi-graph convolutional networks Weighted line graph

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CD301b^(+) macrophage: the new booster for activating bone regeneration in periodontitis treatment

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International Journal of Oral Science 2023年第2期15卷 266-277页

作者： can wang Qin Zhao Chen Chen Jiaojiao Li Jing Zhang Shuyuan Qu Hua Tang Hao Zeng Yufeng Zhang The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of EducationSchool&Hospital of StomatologyWuhan UniversityWuhanChina Institute of Infection and Immunity Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesPR China Medical Research Institute School of MedicineWuhan UniversityWuhanChina Taikang Center for Life and Medical Sciences Wuhan UniversityWuhanChina

Periodontal bone regeneration is a major challenge in the treatment of *** the main obstacle is the difficulty of restoring the regenerative vitality of periodontal osteoblast lineages suppressed by inflammation,via conventional ***301b^(+)macrophages were recently identified as a subpopulation that is characteristic of a regenerative environment,but their role in periodontal bone repair has not been reported.

关键词： period inflammation treatment

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Abnormal metabolism of gut microbiota reveals the possible molecular mechanism of nephropathy induced by hyperuricemia

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Acta Pharmaceutica Sinica B 2020年第2期10卷 249-261页

作者： Libin Pan Pei Han Shurong Ma Ran Peng can wang Weijia Kong Lin Cong Jie Fu Zhengwei Zhang Hang Yu Yan wang Jiandong Jiang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences/Peking Union Medical CollegeBeijing 100050China Insitute of Medicinal Biotechnology Chinese Academy of Medical Sciences/Peking Union Medical CollegeBeijing 100050China

The progression of hyperuricemia disease is often accompanied by damage to renal ***,there are few studies on hyperuricemia nephropathy,especially its association with intestinal *** study combines metabolomics and gut microbiota diversity analysis to explore metabolic changes using a rat model as well as the changes in intestinal flora *** results showed that amino acid metabolism was disturbed with serine,glutamate and glutamine being downregulated whilst glycine,hydroxyproline and alanine being *** combined glycine,serine and glutamate could predict hyperuricemia nephropathy with an area under the curve of *** intestinal flora was also ***,Myroides,Corynebacterium,Alcaligenaceae,Oligella and other conditional pathogens increased significantly in the model group,while Blautia and Roseburia,the shortchain fatty acid producing bacteria,declined *** phylum,family and genus levels,disordered nitrogen circulation in gut microbiota was *** the model group,the uric acid decomposition pathway was enhanced with reinforced urea liver-intestine *** results implied that the intestinal flora play a vital role in the pathogenesis of hyperuricemia ***,modulation of gut microbiota or targeting at metabolic enzymes,i.e.,urease,could assist the treatment and prevention of this disease.

关键词： Hyperuricemia Renal function Gut microbiota Metabolomics Urease

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Effects of Procyanidines on the Expression of STAT3,NFκB Signal Protein and Cytokines in Rats after High-intensity Exercise

Effects of Procyanidines on the Expression of STAT3,NFκB Si...

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华东地区生物化学与分子生物学学会联合会2017年学术交流会暨安徽省生物化学与分子生物学学会理事会议

作者： wang can Beijing Sport University

Objects:To investigate the effects of proanthocyanidins on the expression of *** k B protein and cytokines in exercise training rats,and to study the biochemical mechanism of procysnidines’ s ***:40 eight-week-old male SD rats(weighing 263.27 ± 9.66 g) were divided into four groups randomly:exercise + proanthocyanidins supplementation group,simple exercise intervention group,quiet +proanthocyanidins supplementation group,quiet intervention *** five per cage,free access to food and water,natural circadian rhythm of *** the rats every ***-week intensive swimming training was used as the exercise *** dosage of exercise plus procyanidins supplementation group and quiet + procyanidin supplement group dose of 500 mg/kg BW,simple exercise intervention group and quiet non-intervention group were the same content of water.12 hours after the last *** the rats with sodium pentobarbital(2%) and drew the *** of rats were drawn and then stored in-80℃ ultra-low temperature ***-γ,IL-10,TNF-α,IL-6 content of the spleen were detected by ***3 and NF κB protein was detected by western blot and *** SPSS21.0 to compare the differences between groups,pcant difference,pcant ***:IL-6 index,the simple exercise intervention group had a higher trend than the quiet intervention group(P cantly lower than simple exercise intervention group(P cantly lower than simple exercise intervention group(P <0.01).IFN-γ index,the simple exercise intervention group was higher than the quiet intervention group,with a obvious trend(P <0.01),while the exerci

关键词： procyanidines STAT3 NFκB Cytokines

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Fe/S oxidation-coupled arsenic speciation transformation mediated by AMD enrichment culture under different pH conditions

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Journal of Environmental Sciences 2024年第3期137卷 681-700页

作者： Yu-hang Zhou Wei-xi Huang Zhen-yuan Nie Hong-chang Liu Yue Liu can wang Jin-lan Xia Wen-sheng Shu Key Lab of Biometallurgy of Ministry of Education of China School of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China School of Life Sciences South China Normal UniversityGuangzhou 510631China

Arsenic(As)speciation transformation in acid mine drainage(AMD)is comprehensively affected by biological and abiotic factors,such as microbially mediated Fe/S redox reactions and changes in environmental conditions(pH and oxidation-reduction potential).However,their combined impacts on arsenic speciation transformation remain poorly ***,we explored arsenic transformation and immobilization during pyrite dissolution mediated by AMD enrichment culture under different acidic pH *** results for incubation and mineralogical transformation of solid residues show that in the presence of AMD enrichment culture,pH 2.0,2.5,and 3.0 are more conducive to the formation of jarosites and ferric arsenate,which could immobilize high quantities of dissolved arsenic by adsorption and *** pH conditions significantly affect the initial adsorption of microbial cells to the minerals and the evolution of microbial community structure,further infuencing the biodissolution of pyrite and the release and oxidation process of Fe/*** results of Fe/S/As speciation transformation of the solid residues show that the transformation of Fe,S,and As in solution is mainly regulated by pH and potential values,which imposed significantly different effects on the formation of secondary minerals and thus arsenic oxidation and *** above results indicated that arsenic transformation is closely related to the Fe/S oxidation associated with pyrite bio-oxidation,and this correlation is critically regulated by the pH conditions of the system.

关键词： Acid mine drainage(AMD) pH conditions Fe/S/As speciation transformation Microbial community structure

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Effects of Procyanidines on Cytokines and p38MAPK Protein In Rats after Exercise

Effects of Procyanidines on Cytokines and p38MAPK Protein In...

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中国营养学会第十次特殊营养学术会议

作者： wang can Beijing Sport University

Objects:The aim of this study was to investigate the effects of procyanidines on the expression of cytokines and p38MAPK protein in spleens of rats after exercise, and to provide a basis for the widespread use of procyanidines as a nutritional ***:40 eight-week-old male SD rats were divided into four groups randomly: exercise +procyanidines supplementation group(A group), exercise intervention group(B group), quiet +procyanidines supplementation group(C group), quiet control group(D group). Six-week intensive swimming training was used as the exercise protocol. The dosage of exercise +procyanidines supplementation group and quiet + procyanidines supplementation group were 500 mg/kg BW, exercise intervention group and quiet control group were gavaged with the same content of water. IFN-γ, IL-10, TNF-α, IL-6 content of the spleen were detected by ELISA. The protein of p38MAPK were detected by *** the differences between groups by SPSS21.0 ***:(1) IL-6, the B group had a higher trend than the D group(P cantly lower than B group(P cantly lower than B group(P cantly lower than B group(P <0.01).(4) IL-10, the B group was lower than D group,with a obvious trend(P <0.01).The A group had a higher tendency than B group(P <0.01).(5)p-p38MAPK, the A group had a tendency to decrease compared with the B group. The B group showed a higher trend than the D ***:Strenuous exercise caused the disorder of cytokines metabolism,the supplementation of procyanidines may regulate the expression of proinflammatory cytokines and anti-inflammatory cytokines,so as to reduce the disorder of cytokines caused by exercise and to promote the balance beteween the proinflammatory cytokines a

关键词： procyanidines p38MAPK IL-6 TNF-α

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Effects of Exercise on the Balance of Th1Th2 in Rats and the Intervention of the Procyanidines

Effects of Exercise on the Balance of Th1Th2 in Rats and the...

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第十一届全国免疫学学术大会

作者： wang can 北京体育大学

Purpose: The aim of this study was to observe the effects of exercise on the regulation of Th1/Th2 balance in rats, and to explore the effects of the intervention of the procyanidines on ***: With random number table,40 SPF SD rats were randomly divided into 4 groups, the sedentary control group(SC group), the sedentary nutrition group(SN group), the exercise control group(EC group) and the exercise nutrition group(EN group).The sedentary nutrition and exercise nutrition groups were gavaged with 300mg/(kg·d) dose procyanidines,the exercise control and exercise nutrition groups were assigned to an increasing load swimming training for 6 weeks.24 hours after the last training,we determined the content of IFN-γ、TNF-α、IL-4 and IL-10 in blood serum by *** data was examined by SPSS 21.0 statistical ***:(1) The results showed that the content of IFN-γ in EC group was significantly lower than SC group(pcantly higher than EC group(pcantly higher than EC group(pcantly higher than SC group(pcantly lower than EC group(pcantly higher than SC group(pcantly lower than EC group(p<0.01).Conclusions: The results indicated that high intensity exercise could cause the Th1/Th2 imbalance,thus affect the immune *** supplement of procyanidines could regulate the immune function by regulating the immune factors in serum and improving the Th1/Th2 balance.

关键词： Th1/Th2 TNF-α IL-10 procyanidines

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