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Mechanical loading induced expression of bone morphogenetic protein-2, alkaline phosphatase activity, and collagen synthesis in osteoblastic MC3T3-E1 cells

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Chinese Medical Journal 2012年第22期125卷 4093-4097页

作者： LU hong-fei Mai Zhi-hui XU Ye wang Wei ai hong Sun Yat Sen Univ Dept Stomatol Hosp 3 Guangzhou 510630 Guangdong Peoples R China Second Hosp Chao Yang Dist Dept Stomatol Beijing 100022 Peoples R China

Background Bone morphogenetic protein （BMP）-2, alkaline phosphatase （ALP）, and collagen type I are known to play a critical role in the process of bone remodeling. However, the relationship between mechanical strain and the expression of BMP-2, ALP, and COL-I in osteoblasts was still unknown. The purpose of this study was to investigate the effects of different magnitudes of mechanical strain on osteoblast morphology and on the expression of BMP-2, ALP, and COL-I. Methods Osteoblast-like cells were flexed at four deformation rates （0, 6%, 12%, and 18% elongation）. The expression of BMP-2 mRNA, ALP, and COL-I in osteoblast-like cells were determined by real-time quantitative reverse transcription polymerase chain reaction, respectively. The results were subjected to analysis of variance （ANOVA） using SPSS 13.0 statistical software. Results The cells changed to fusiform and grew in the direction of the applied strain after the mechanical strain was loaded. Expression level of the BMP-2, ALP, and COL-I increased magnitude-dependently with mechanical loading in the experimental groups, and the 12% elongation group had the highest expression （P 〈0.05）. Conclusion Mechanical strain can induce morphological change and a magnitude-dependent increase in the expression of BMP-2, ALP, and COL-I mRNA in osteoblast-like cells, which might influence bone remodeling in orthodontic treatment.

关键词： mechanical strain, osteoblasts bone morpho enetic protein-2 alkaline phosphatase collagen type I

Cloning and Expression Pattern of a Gene Encoding a Putative Plastidic ATP/ADP Transporter from Helianthus tuberosus L.

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Journal of Integrative Plant Biology 2005年第9期47卷 1123-1132页

作者： Kun MENG Tuan-Jie CHANG Xiang LIU Song-Biao CHEN Yong-Qin wang ai-Jun SUN hong-Lin XU Xiao-Li WEI Zhen ZHU Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China

Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolated from the tuber-cDNA library of H. tuberosus for the first time. The predicted HtAATP protein was judged as a plastidic ATP/ADP translocator protein from its high homology at the amino acid sequence level to the two Arabidopsis thaliana plastidic ATP/ADP translocator proteins AATP1 and AATP2 （84.8% and 79.9% identity, respectively）. Amino acid sequence analysis of the primary structure of HtAATP revealed that it belonged to the plastidic ATP/ADP transporter family. Hydropathy prediction indicated that HtAATP gene product is a highly hydrophobic membrane protein that contains 10 transmembrane domains to form a spanning topology. Southern blotting analysis showed that the HtAATP gene is a single-copy gene in the H. tuberosus genome. Tissue distribution analysis showed that the HtAATP gene is prominently expressed in sink tissues. A stable expression pattern in tubers at different developmental stages implies an active involvement of HtAATP during carbohydrate formation.

关键词： expression pattern Helianthus tuberosus plastidic ATP/ADP transporter.

Effect of epidermal growth factor and dexamethasone on fetal rat lung development

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Chinese Medical Journal 2009年第17期122卷 2013-2016页

作者： MA Lian wang ai-hong Law Frieda HE hong-yan MA Gui-xia wang hong-wu LIN Li-min Department of Pediatrics Second Affiliated Hospital of ShantouUniversity Medical College Shantou Guangdong 515041 China Department of Surgery First Affiliated Hospital of ShantouUniversity Medical College Shantou Guangdong 515144 China Shantou University Medical College Shantou Guangdong515041 China

Background Epidermal growth factor （EGF）, a mitogenic polypeptide that binds to cell surface receptors, is an important regulator of cell differentiation and fetal lung surfactant synthesis. We investigated the preventive and therapeutic effects of EGF in respiratory distress syndrome, by administering EGF and dexamethasone （Dex） to mother rat before delivery. Methods Six female Sprague-Dawley （SD） rats were assigned to three groups （2 rats each）; EGF or Dex was given to pregnant rats （EGF group and Dex group, respectively） from gestational day 16 to day 18 by intraperitoneal injection, while the group with normal saline injection was used as negative controls. Fetal rats were taken out of womb by hysterotomy on day 19 of pregnancy, then 24 fetal rats were randomly chosen from each group. Their body weights were measured, and pulmonary surfactant protein-A and -B （SP-A and SP-B） antigens were determined by immunohistochemical staining in each group. The histologic structure was examined under a light microscope, a light microscopic image system or an electron microscope. Results The expressions of SP-A and SP-B could be detected in each group. A significant difference was observed for SP-A and SP-B in the EGF and Dex groups compared with the control group （P 〈0.01）. Image analysis showed that the relative values of air space area and interalveolar septa area in the EGF and Dex groups were significantly greater than those in the control group （P 〈0.01）, while no significant difference was found between the two groups （P 〉0.05）. The ultrastructural features of fetal lungs showed that the number of alveolar type [I cells containing lamellar bodies in the EGF and Dex groups was apparently increased compared with that in the control group. The mean body weight of fetus from the Dex group was smaller than that from the control group （（1.3192＋0.0533） g, （1.3863_＋0.0373） g）, but there was no significant difference between the EGF group and the control group （（1.3986_＋0.0730

关键词： epidermal growth factor dexamethasone fetal lung

Overexpression of dishevelled-1 attenuates wortmannin-induced hyperphosphorylation of cytoskeletal proteins in N2a cell

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Acta Pharmacologica Sinica 2005年第6期26卷 679-684页

作者： Hai-hong wang, ai-hong ZHANG, Ling-qiang ZHU, Qun wang, Jian-zhi wang~2 Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China DepartmentofPathophysiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China

aim: To investigate the effect of dishevelled-1 (DVL-1) on wortmannin-induced Alzheimer-like hyperphosphorylation of cytoskeletal proteins in mouse neuroblastoma 2a (N2a) cells. Methods: Cultured N2a cells were transitorily transfected with DVL-1 expression plasmid using Lipofectamine鈩?2000. Western blot and immunofluorescence microscopy were used to measure the phosphorylation of neurofilament and tau. Results: Level of phosphorylated neurofilament at SMI31 epitope and phosphorylated tau determined by PHF-1 was increased at 1 h and 3 h and back to normal at 6 h after wortmannin 1 |imol/L treatment. The highest level of phosphorylated neurofilament and phosphorylated tau was seen at 1 h and 3 h after wortmannin treatment, respectively. When DVL-1 protein was overexpressed, the hyperphosphorylation of neurofilament at SMI31 and SMI32 epitopes and tau atPHF-l(Ser-396/404),M4(Thr-231/Ser-235),andTau-l(Ser-198/199/202)epitopes was attenuated. Conclusion: Overexpression of mouse DVL-1 protein inhibits wortmannin-induced hyperphosphorylation of neurofilament and tau in N2a cells.

关键词： Alzheimer disease tau proteins neurofilament wortmannin dishevelled-1

Clinicopathological features and treatment of gastrointestinal schwannomas

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World Journal of Gastroenterology 2025年第5期31卷 32-42页

作者： Peng-Cheng Zhang Shu-Hui wang Jun Li Jing-Jie wang hong-Tan Chen ai-Qing Li Department of Gastroenterology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Epidemiology&Health Statistics School of Public HealthSchool of MedicineZhejiang UniversityHangzhou 310058Zhejiang ProvinceChina Department of Pathology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina

BACKGROUND Gastrointestinal schwannomas(GIS)are rare neurogenic tumors arising from Schwann cells in the gastrointestinal *** on GIS are limited to small case reports or focus on specific tumor sites,underscoring the diagnostic and thera-peutic challenges they *** To comprehensively examine the clinical features,pathological characteristics,treatment outcomes,associated comorbidities,and prognosis of *** The study population included patients diagnosed with GIS at the First Affiliated Hospital,Zhejiang University School of Medicine,between June 2007 and April *** were retrospectively collected and analyzed from medical records,including demographic characteristics,endoscopic and imaging findings,treatment modalities,pathological evaluations,and follow-up *** In total,229 patients with GIS were included,with a mean age of 56.00 years and a male-to-female ratio of 1:*** mean tumor size was 2.75 cm,and most(76.9%)were located in the ***,6.6%of the patients had other malig-nant *** imaging and endoscopy frequently misdiagnosed GIS as gastrointestinal stromal ***,accurate preoperative diagnosis was achieved using endoscopic ultrasound-guided fine-needle aspiration combined with immunohistochemical analysis,in which S100 and SOX-10 markers were mostly *** tumors were typically managed with endoscopic resection,while larger lesions were treated with surgical ***-up results showed that most patients experienced favorable *** Preoperative diagnosis of GIS via clinical characteristics,endoscopy,and imaging examinations remains challenging but *** therapy provides a minimally invasive and effective option for patients.

关键词： Gastrointestinal schwannoma Diagnosis Treatment Endoscopictherapy Complications

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略论农民增收的障碍及对策——以河南为例

经济问题 2008年第11期 93-96,112页

作者：王爱红濮阳职业技术学院工商管理系河南濮阳457000

全面建设小康社会的重点、难点、潜力在农村,而关键在于增加农民收入。当前农民因结构不合理、非农经济发展缓慢、农民负担重、农业投入不足、农民素质不高、政府服务不到位等诸多因素影响,农民收入存在多元化、不均衡、不稳定等问题,... 详细信息

全面建设小康社会的重点、难点、潜力在农村,而关键在于增加农民收入。当前农民因结构不合理、非农经济发展缓慢、农民负担重、农业投入不足、农民素质不高、政府服务不到位等诸多因素影响,农民收入存在多元化、不均衡、不稳定等问题,农民增收困难。探讨阻碍农民增收的原因并提出实现农民增收的对策。

关键词：农民增收障碍因素对策

The secondary periodic elementβ_(p^(2)/p^(2)-1)and its applications

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Science China Mathematics 2025年第1期68卷 207-222页

作者： Jianguo hong Xiangjun wang Yu Zhang Science College Shijiazhuang UniversityShijiazhuang 050035China School of Mathematical Sciences Nankai UniversityTianjin 300071China Center for Applied Mathematics Tianjin UniversityTianjin 300072China

Let p≥7 be a *** prove thatβ_(p^(2)/p^(2)-1)survives to E_(∞)in the Adams-Novikov spectral ***,using the Thom mapΦ:■,we can see that h0h3 also survives to E_(∞)in the classical Adams spectral *** an application ... 详细信息

关键词： stable homotopy groups of spheres Adams-Novikov spectral sequence in nite descent method

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我国农产品物流发展的对策与建议

中国市场 2008年第45期 62-64页

作者：王爱红濮阳职业技术学院河南濮阳457000

解决"三农"的核心是增加农民收入。当今,农民收入为何难提高?原因是多方面的,其中一个重要原因是农民生产的农产品由于"卖难",无法实现其价值或低值出售,致使农民不能增收。实践证明大力发展农产品现代物流,是解决农产品"卖难"的有效举... 详细信息

解决"三农"的核心是增加农民收入。当今,农民收入为何难提高?原因是多方面的,其中一个重要原因是农民生产的农产品由于"卖难",无法实现其价值或低值出售,致使农民不能增收。实践证明大力发展农产品现代物流,是解决农产品"卖难"的有效举措,是增加农民收入的有效途径。

关键词：农产品物流发展对策建议

Tauroursodeoxycholic acid targets HSP90 to promote protein homeostasis and extends healthy lifespan

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Science China(Life Sciences) 2025年第2期68卷 416-430页

作者： Jia-Yu Liu Yao wang Yue Guo Run-Qi Zheng Yun-Ying wang Yan-Yan Shen Yan-hong Liu ai-Ping Cao Rui-Bo wang Bo-Yang Xie Shuai Jiang Qiu-Ying Han Jing Chen Fang-Ting Dong Kun He Na wang Xin Pan Tao Li Tao Zhou ai-Ling Li Qing Xia Wei-Na Zhang Nanhu Laboratory National Center of Biomedical AnalysisBeijing 100850China

As the elderly population expands,the pursuit of therapeutics to reduce morbidity and extend lifespan has become increasingly *** an FDA-approved drug for chronic cholestatic liver diseases,tauroursodeoxycholic acid(TUDCA),a natural bile acid,offers additional health benefits beyond liver ***,we show that TUDCA extends the lifespan and healthspan of ***,oral supplementation of TUDCA improves fitness in old mice,including clinically relevant phenotypes,exercise capacity and cognitive ***,TUDCA treatment drives broad transcriptional changes correlated with anti-aging ***,we discover that TUDCA targets the chaperone HSP90 to promote its protein refolding *** collaboration further alleviates aging-induced endoplasmic reticulum(ER)stress and facilitates protein homeostasis,thus offering resistance to *** summary,our findings uncover new molecular links between an endogenous metabolite and protein homeostasis,and propose a novel anti-aging strategy that could improve both lifespan and healthspan.

关键词： tauroursodeoxycholic acid lifespan healthspan protein homeostasis