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Induction of Apoptosis by L-NMMA,via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells

Biomedical and Environmental Sciences 2006年第4期19卷 285-291页

作者： YONG-zhong wang ZHEN-qing FENG Department of Pathology Nanjing Medical University Nanjing 210029 Jiangsu China

Objective To investigate the apoptosis-inducing effect of endogenous nitric oxide （NO） suppression in gastric cancer cells and its mechanisms. Methods Apoptosis of gastric cancer cells was detected by flow cytometry. Expression of phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL1 in gastric cancer cells was analyzed using Western blotting. Immunofluorescence assay was performed to localize the intracellular phosphorylated FKHRL1 （thr-32, ser-253） and FKHRL 1. Transfection of FKHRL1-HA wild type and mutant FKHRL1-HA T32A constructs was performed by lipofectamine plus reagent. NO generation was determined by Griess reaction. Results Gastric cancer cells were significantly apoptotic after treatment with NG-monomethyl-L-arginine （L-NMMA, a nitric oxide synthase inhibitor）, compared with the control （P〈0.01）. The apoptosis of gastric cancer cells induced by L-NMMA was dose-dependent and time-independent. However, the Z-DEVD-fmk, a caspase-3, 6, 7, 8, l0 inhibitor, did not prevent the apoptosis. The immunofluorescence assays showed that FKHRL1 protein was strongly expressed in the nucleu and p-FKHRL1 thr-32 protein was ：strongly expressed in the cytoplasm of SGC-7901 cells when endogenous nitric oxide generation was blocked by L-NMMA, but no change in FKHRL1 ser-253 phosphorylation. Nevertheless, ROCK protein was strongly expressed in p-FKHRL1 thr-32-positive SGC-7901 cells. The wortmannin, an inhibitor of phosphoinositol-3-OH kinase （PI3K）, did not block the phosphorylated FKHRL1 thr-32 protein induced by L-NMMA. However, Y-27632, a specific inhibitor of the protein kinase ROCK, significantly blocked apoptosis induced by phosphorylated FKHRL1 thr-32 （P〈0.01）, which was mediated by L-NMMA. A significant decrease in NO generation （P〈0.01） and a significant increase in apoptosis （P〈0.01） were observed when FKHRL1-HA wild-type cells were transfected, which caused increased FKHRL1 thr-32 phosphorylation. Conclusions L-NMMA triggers gastric carcinoma cell apoptosis, possibly

关键词： Gastric carcinoma Nitric oxide FKHRL1 Apoptosis Signaling

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奇偶q-变形相干态的高阶压缩效应

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物理学报 2001年第4期50卷 90-92页

作者：汪仲清石油大学应用物理系东营257061

研究了奇偶 q 变形相干态中光场的高阶压缩效应 .数值计算结果发现 ,奇偶 q 变形相干态中光场的高阶压缩效应与参数值 q有关 ,当参数q取偏离 1较大的值时 ,q 变形奇偶相干态均可具有奇次方阶压缩效应却无偶次方阶压缩效应。

关键词：光场 q-变形相干态高阶压缩效应振幅

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k玻色子q相干态的反聚束效应(英文)

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高能物理与核物理 2002年第6期26卷 576-581页

作者：汪仲清石油大学应用物理系山东东营257061

研究了q变形湮没算符高次幂 (akq,k≥ 3 )本征态的反聚束效应 ,并就k =3的情况用数值计算方法研究了q变形参数对该效应的影响 .结果表明 ,当q变形相干态中谐振子的强度x=|z| 2 在某些区间内取值时 ,akq 的本征态将呈现反聚束效应 ,并且... 详细信息

研究了q变形湮没算符高次幂 (akq,k≥ 3 )本征态的反聚束效应 ,并就k =3的情况用数值计算方法研究了q变形参数对该效应的影响 .结果表明 ,当q变形相干态中谐振子的强度x=|z| 2 在某些区间内取值时 ,akq 的本征态将呈现反聚束效应 ,并且这一效应明显地受到q参数的影响 .当参数q取定时 ,随着q变形光场强度的变大 ,该光场的光子数涨落在经典 (或量子 )和量子 (或经典 )特性之间交替地变化 .

关键词： κ玻色子 q相干态 q变形本征态反聚束效应量子光学湮没算符高次幂谐振子

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Ionic liquid modified GC electrode for the direct electrochemistry of chloroperoxidase

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Chinese Chemical Letters 2008年第12期19卷 1483-1486页

作者： Liu Yang Xia Qin Wu Rong wang zhong qing Lu Wen Jing Hou He Xing Li Life and Environment Science College Shanghai Normal University 100 Guilin Road Shanghai 200234 PR China

Chloropcroxidase （CPO） was immobilized by konjac glucomannan （KGM） on the 1-butyl-3-methyl imidazolium tetrafluoroborate [BMIM][BF4]/Nafion modified glassy carbon eloctrode. The electrochemical behaviors of the immobilized CPO were investigated by cyclic voltammetry. The results showed that CPO was successfully immobilized on the GCE and underwent fast direct electron transfer reactions with the formal potential at -0.3 V vs. SCE. The modified electrode showed a good catalytic activity for elcctrocatalytical reduction of O2 and H2O2.

关键词： Ionic liquid Chloroperoxidase Electroeatalysis Direct electrochemistry

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Effects of Zishen Yutai Pills on in vitro Fertilization-Embryo Transfer Outcomes in Patients with Diminished Ovarian Reserve:A Prospective,Open-Labeled,Randomized and Controlled Study

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Chinese Journal of Integrative Medicine 2023年第4期29卷 291-298页

作者： LI Xiu-fang wang zhong-qing XU Hai-yan LIU Hong SHENG Yan XU Jin LI Yuan-mei LIAN Fang The First Clinical College Shandong University of Traditional Chinese MedicineJinan 250014China Center for Reproductive Medicine Shandong UniversityJinan 250021China Medical College of Optometry and Ophthalmology Shandong University of Traditional Chinese MedicineJinan 250014China Department of Traditional Chinese Medicine Jinan Golden Time Health Nursing HospitalJinan 250000China Reproductive and Genetic Center of Integrated Traditional and Western Medicine Affiliated Hospital of Shandong University of Traditional ChineseJinan 250011China Medicine

Objective:To explore the effects of Zishen Yutai Pills(ZYPs)on the quality of oocytes and embryos,as well as pregnancy outcomes in patients with diminished ovarian reserve(DOR)receiving in vitro fertilizationembryo transfer(IVF-ET).The possible mechanisms,involving the regulation of bone morphogenetic protein 15(BMP15)and growth differentiation factor 9(GDF9),were also ***:A total of 120 patients with DOR who underwent their IVF-ET cycle were randomly allocated to 2 groups in a 1:1 *** patients in the treatment group(60 cases)received ZYPs from the mid-luteal phase of the former menstrual cycle by using gonadotropin-releasing hormone(Gn RH)antagonist *** patients in the control group(60 cases)received the same protocol but without *** primary outcomes were the number of oocytes retrieved and high-quality *** outcomes included other oocyte or embryo indices as well as pregnancy *** events were assessed by comparison of the incidence of ectopic pregnancy,pregnancy complications,pregnancy loss,and preterm *** of BMP15 and GDF9 in the follicle fluids(FF)were also quantified with enzymelinked immunosorbent ***:Compared with the control group,the numbers of oocytes retrieved and high-quality embryos were significantly increased in the ZYPs group(both P0.05).The administration of ZYPs did not increase the incidence of adverse *** expressions of BMP15 and GDF9 in the ZYPs group were significantly up-regulated compared with the control group(both P<0.05).Conclus

关键词： Zishen Yutai Pills diminished ovarian reserve in vitro fertilization-embryo transfer oocytes embryos

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Research on Digital Models for Product Design and Manufacture

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厦门大学学报（自然科学版） 2002年第S1期41卷 155-156页

作者： wang zhong-qing, GUO Chun-liang (East China Jiaotong University, Nanchang 330013, China)

CAD/CAM integrate technology has been drawn to much a ttention and becomes one of the focus of the international academic and commerci al research, Through efforts nearly 10 years, remarkable progress has been made in the 3-dimension feature parametric modeling. UG, SOLIDWORK and MDT apply the se technology with respective features. However, no distinct development has bee n made in the digital models from geometric feature to manufacture feature. Prod uct information expression and the basic model frame of data exchange have been proposed based on STEP standards, but it is quite difficult to some extent ensur e the operability, due to the complexity in application. The models addressed in this paper divide the shared digital models (not data models) for product desig n and manufacture into five parts: ①Digital product 3D molding design; ②Digit al product technology process design; ③Digital product assembly design; ④Digit al product manufacture process design; ⑤Digital product management design. The resource model should not be included. And It should be a peripheral model i n product design; which is the innovative viewpoint of this paper. The core of above-mentioned five models is to access the digital model from the solid unit feature. Product definition should be determined on the basis of solid unit, and solid unit should follow the rules of "generation of machining processing", Therefore, the author also proposed the Constructing Cutting Geomet ry Method (CCG) in IMCC’2000. In this way, the integration of "forming - processing - assembling - machining - management" can be obtained. This is th e core originality of the paper.

关键词： product digitization solid unit technique CAD CAPP CAM

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Study on phase diagrams and properties of solutions in ternary systems Li+,K+(Mg2+)/SO42--H2O at 25℃

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Chinese Journal of Chemistry 1995年第2期13卷 112-117页

作者： LI,Bing LI, Jun FANG, Chun-Hui wang, qing-zhong SONG, Peng-Shengqinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining, qinghai 810008, China qingkai Institute of Salt Lakes Chinese Academy of Sciences Xining Qinghai 810008 China

Solubilities of ternary systems Li+,K+/SO42--H2O (1) and Li+,Mg2+/SO42-H2O (2) were investigated by isothermal method at 25℃. Physico-chemical properties of solutions, such as density, refractive index, viscosity, conductivity and pH, were determined. Phase diagram of the system (1) consists of three solubility branches and three crystallization fields corresponding to K2SO4, Li2SO4.H2O and LiKSO4. LiKSO4 is an incongruent compound, and its transition point is estimated graphically to be 45.5-46.0℃. No solid solution of LiKSO4 with Li2SO4.H2O was found in the system. The system (2) is a simple eutonic type. Pitzer model of electrolyte solution was used to check the obtained solubilities. Data comparison gives good agreement. Two equations were used to correlate density, refractive index of the solution with its composition. Differences between measured and calculated values are less than 0.6% for density, 0.15% for the latter.

关键词： Salt-water system solubility of Li salt phase diagram.

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q变形对相干态的相位概率分布特性

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高能物理与核物理 2006年第3期30卷 273-276页

作者：汪仲清段昌奎重庆邮电学院光电工程学院

通过推广Pegg和Barnett的相位算符和相位态到q变形的双模情况,应用数值计算研究了q变形对相干态的相位概率分布特性．结果表明,q变形对相干态的相位概率分布受到相位参数、q参数和参数|ξ|的调节,从而反映出不同的量子相干特性．

关键词： q变形对相干态相位概率分布量子干涉特性

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激发压缩真空态的相位概率分布(英文)

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光子学报 2008年第3期37卷 589-593页

作者：汪仲清万鹏重庆邮电大学光电工程学院重庆400065

应用Pegg和Barnett提出的相位算符和相位态理论,研究了激发压缩真空态的相位概率分布特性.数值计算结果表明,激发压缩真空态的相位概率分布主要受到相位参量和压缩参量的调节,相位参量使相位概率分布呈峰值结构,压缩参量的变化将影响相... 详细信息

应用Pegg和Barnett提出的相位算符和相位态理论,研究了激发压缩真空态的相位概率分布特性.数值计算结果表明,激发压缩真空态的相位概率分布主要受到相位参量和压缩参量的调节,相位参量使相位概率分布呈峰值结构,压缩参量的变化将影响相位概率分布的峰值强度,此外激发光子数对相位概率分布也有较大的影响.

关键词：激发压缩真空态相位态相位概率分布量子干涉效应

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小鼠精原干细胞在三种培养基中的生长行为

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中国应用生理学杂志 2009年第2期25卷 286-288页

作者：王庆忠潍坊学院生物工程学院山东潍坊261061

目的:建立小鼠精原干细胞(SSCs)的体外长期培养体系。方法:用分别添加了等量的胶质细胞源神经营养因子(GDNF)、可溶性GFRα1和bFGF的DMEM/F12、KSR和StemPro-34 SFM三种无血清培养基和MEF饲养层分别培养经差异贴壁分选富集的小鼠SSCs,... 详细信息

目的:建立小鼠精原干细胞(SSCs)的体外长期培养体系。方法:用分别添加了等量的胶质细胞源神经营养因子(GDNF)、可溶性GFRα1和bFGF的DMEM/F12、KSR和StemPro-34 SFM三种无血清培养基和MEF饲养层分别培养经差异贴壁分选富集的小鼠SSCs,通过形态观察、标志基因的RT-PCR和免疫细胞化学分析检测其SSCs本原。结果:DMEM/F12与KSR可支持小鼠SSCs在体外存活6-7 d,而StemPro-34 SFM能能维持SSCs体外增值一个月。结论:StemPro-34 SFM支持小鼠SSCs的体外增殖。

关键词：精原干细胞,无血清培养基,培养,小鼠

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