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Identification of two Major Histocompatibility(MH) Class Ⅱ A Genes and Their Association to Vibrio anguillarum Infection in Half-smooth Tongue Sole(Cynoglossus semilaevis)

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Journal of Ocean University of China 2012年第1期11卷 32-44页

作者： LI Chunmei wang Xubo ZHANG Quanqi wang zhigang QI Jie YI Qilin LIU Zhipeng wang Yanan YU Haiyang College of Marine Life Sciences Key Laboratory of Marine Genetics and BreedingMinistry of EducationOcean University of ChinaQingdao 266003P.R.China

Major histocompatibility complex class II antigens are important in vertebrate immune *** the present study,the full cDNA sequence of class II A gene was synthesized by RACE-PCR from half-smooth tongue sole(Cynoglossus semilaevis),and its open reading frame(ORF) polymorphism was *** whole cDNA sequence was 992 bp in length,including the ORF with 717 ***-five alleles were identified and clustered into two distinct groups according to the specific nucleotides/amino acids in specific *** alleles belonged to Cyse-DAA while fourteen alleles belonged to *** Cyse-DAA alleles were observed in one individual,and three to five Cyse-DBA alleles were observed in each of the three detected individuals,which indicated that at least two loci existed in each ***,in order to study the function of the alleles in resistance to infection,200 individuals were intraperitoneally injected with Vibrio anguillarum and the first 20 dead individuals and 20 surviving ones were selected for genotype ***-six alleles were identified among the 40 ***-nine alleles belonged to Cyse-DAA and the other 27 alleles belonged to *** alleles were selected for studying their function in resistance to *** Cyse-DAA*0201,Cyse-DAA*1101,Cyse-DBA*0401,Cyse-DBA*1102,Cyse-DBA*1801 and Cyse-DBA*2201 were identi-fied only in surviving individuals,while alleles Cyse-DAA*0901,Cyse-DBA*1101 and Cyse-DBA*1401 occurred more frequently in dead *** study confirmed the existence and polymorphism of two class II A genes as well as the relationship between alleles of class II A genes and disease susceptibility/resistance in half-smooth tongue sole.

关键词： major histocompatibility polymorphism Cyse-DAA Cyse-DBA allele Vibrio anguillarum

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基于层次化P2P协议的网格空间数据库系统模型

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武汉大学学报（信息科学版） 2008年第12期33卷 1233-1236,1258页

作者：孟令奎张文 Frank zhigang wang 武汉大学遥感信息工程学院武汉市430079 剑桥-克兰菲尔德高性能计算设施网格计算中心 AMAC/SOE克兰菲尔德大学英国Bedfordshire MK43OAL

针对传统空间数据库技术的不足,充分结合网格空间数据管理的新特点,提出了一种适合于网格环境的空间数据库系统模型(grid peer-spatial database management system,GPeer-SDBMS)。该模型运用P2P协议Tapestry构建了基于网格的层次化分... 详细信息

针对传统空间数据库技术的不足,充分结合网格空间数据管理的新特点,提出了一种适合于网格环境的空间数据库系统模型(grid peer-spatial database management system,GPeer-SDBMS)。该模型运用P2P协议Tapestry构建了基于网格的层次化分布式空间数据库,不仅利用数据库的模式差异将网格空间数据库划分为由相同模式节点组成的多个虚拟节点集合,实现了空间数据的分布式存储,而且还对查询算法进行了有效的改进,并通过实验验证了查询算法的高效性。

关键词：网格空间数据库 P2P Tapestry 分布式

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Locally differentially private frequency distribution estimation with relative error optimization

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Frontiers of Computer Science 2024年第5期18卷 227-229页

作者： Ning wangI Yifei LIU zhigang wang Zhiqiang WEI Ruichun TANG Peng TANG Ge YU Cyberspace Institute of Advanced Technology Guangzhou UniversityGuangzhou 510006China School of Computer Science and Technology Ocean University of ChinaQingdao 266100China Key Laboratory of Cryptologic Technology and Information Security(Ministry of Education) Shandong UniversityQingdao 266071China School of Computer Science and Technology Northeastern UniversityShenyang 110819China

This paper tackles the problem of estimating the frequency distribution on the crowdsourced multidimensional categorical data under local differential privacy(LDP).Although the frequency estimation problem under LDP[1]has attracted a lot of attention in recent years,currently,to our knowledge,the existing works are all devoted to optimizing the absolute error,rather than relative *** the work for the former,the one targeting at the latter should take the true frequency distribution into consider and design true frequency distribution-sensitive data collection protocol so that the skewed distribution,which involves smaller frequency in high probability,is with less ***,it is challenging to fulfill such a requirement,since the true frequency distribution is private information and not available.

关键词： distribution estimation error

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Driving High-Quality Development Through Grcater Self-reliance and Strength in Science and Technology

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Qiu Shi 2023年第5期15卷 48-55页

作者： wang zhigang 不详

Achieving high-quality development is the primary task in building a modern socialist country and an essential requirement of Chinese *** accomplish this task,we must regard science and technology(S&T)as our primary productive force,talent as our primary resource,and innovation as our primary driver of *** must make S&T innovation an integral element of overall economic and social development and cultivate new engines for sustainable growth.

关键词： productive modernization sustainable

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Forming Limit Stress Diagram Prediction of Aluminum Alloy 5052 Based on GTN Model Parameters Determined by In Situ Tensile Test

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Chinese Journal of Aeronautics 2011年第3期24卷 378-386页

作者： HE Min LI Fuguo wang zhigang School of Materials Science and Engineering Northwestern Polytechnical University Xi 'an 710072 China Department of Mechanical Science and Engineering Xuzhou Institute of Technology Xuzhou 221008 China Department of Aircraft Structure Design and Research the First Aircraft Institute Aviation Industry Corporation of China Xi 'an 710089 China

The conventional forming limit diagram （FLD） is described as a plot of major strain versus minor strain. However, FLD is dependent on forming history and strain path. In the present study, a forming limit stress-based diagram （FLSD） has been adopted to predict the fracture limit of aluminum alloy （AA） 5052-O1 sheet. Nakazima test is simulated by plastic constitutive formula derived from the modified Gurson-Tvergaard-Needleman （GTN） model. An in situ tensile test with scanning electron microscope （SEM） is proposed to determine the parameters in GTN model. The damage evolution is observed and recorded, and the parameters of GTN model are identified through counting void fraction at three damage stages of AA5052-O 1. According to the experimental results, the original void volume fraction, the volume fraction of potential nucleated voids, the critical void volume fraction, the void volume fraction at the final failure of material are assigned as 0.002 918, 0.024 9, 0.030 103, 0.048 54, respectively. The stress and strain are obtained at the last loading step before crack. FLSD and FLD of AA5052-O 1 are plotted. Compared with the experimental Nakazima test and uniaxial tensile test, the predicted results show a good agreement. The parameters determined by in situ tensile test can be applied to the research of the forming limit for ductile metals.

关键词： forming limit stress diagram GTN model in situ tensile test void damage aluminum alloy 5052-O1 sheet metal forming

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Field Detection of Chlorophyll-a Concentration in the Sea Surface Layer by an Airborne Oceanographic Lidar

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Journal of Ocean University of China 2008年第1期7卷 108-112页

作者： LIU Zhishen MA Sen wang Xin LI zhigang Ocean Remote Sensing Institute Ocean University of China Qingdao 266003 R R. China

An airborne oceanographic lidar, with a frequency-tripled Q-switched Nd： YAG laser of 355 nm, has been designed to measure chlorophyll-a （Chl-a） concentration in the sea surface layer by the Ocean Remote Sensing Institute, OUC. The field experiment was carried out in the bay which is located south of the Liaodong Peninsula on the 10th of September 2005. After the flight, the raw data were processed and analyzed by the fluorescence-to-Raman ratio method with seawater attenuation coefficients calculated from signal profiles. The results of Chl-a concentration sea water were also compared with those of Chl-a concentration by measurements by lidar are shown. The measurements in clear a Moderate Resolution Imaging Spectroradiometer （MODIS）.

关键词： airborne oceanographic lidar fluorescence Raman seawater attenuation coefficients Chl-a MODIS

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Design optimization and testing of a morphing leading-edge with a variable-thickness compliant skin and a closed-chain mechanism

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Chinese Journal of Aeronautics 2024年第7期37卷 285-300页

作者： zhigang wang Xiasheng SUN Yu YANG Wenjie GE Daochun LI Jinwu XIANG Panpan BAO Qi WU Andrea DA RONCH School of Aeronautical Science and Engineering Beihang UniversityBeijing 100191China National Key Laboratory of Strength and Structural Integrity Aircraft Strength Research Institute of ChinaXi’an 710065China School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai 200240China School of Mechanical Engineering Northwestern Polytechnical UniversityXi’an 710072China Faculty of Engineering and Physical Sciences University of SouthamptonSouthampton SO171JBUK

Climate warming and the increased demand in air travels motivate the aviation industry to urgently produce technological *** of the most promising innovations is based on the smoothly continuous morphing leading-edge *** study proposes a two-step process for the design of a morphing leading-edge,including the optimization of the outer variable-thickness composite compliant skin and the optimization of the inner kinematic *** the compliant skin design,an optimization of the variable thickness composite skin is proposed based on a laminate continuity model,with laminate continuity constraint and other manufacturing *** laminate continuity model utilizes a guiding sequence and a ply-drop sequence to describe the overall stacking sequence of plies in different thickness regions of the complaint *** the inner kinematic mechanism design,a coupled four-bar linkage system is proposed and optimized to produce specific trajectories at the actuation points on the stringer hats of the compliant skin,which ensures that the compliant skin can be deflected into the aerodynamically optimal ***,a morphing leading-edge is manufactured and *** results are compared with numerical predictions,confirming the feasibility of the morphing leading-edge concept and the overall proposed design approach.

关键词： Optimization Design Morphing wing Compliant skin Kinematics Leading-edge Control surfaces

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Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS-LRR Protein Class Gene in Apple （Golden Delicious）

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Molecular Plant 2014年第1期7卷 218-230页

作者： Chao Ma You Lu Songlin Bai Wennan Zhang Xuwei Duan Dong Meng zhigang wang Aide wang Zongshan Zhou Tianzhong Li Laboratory of Fruit Cell and Molecular Breeding China Agricultural University Beijing 100193 China Research Institute of Pomology Chinese Academy of Agricultural Sciences Xingcheng Liaoning 125100 China College of Horticulture Shenyang Agricultural University Shenyang 110866 China

MicroRNA （miRNA） has emerged as an important regulator of gene expression in plants. 146 miRNAs were identified from apple （Malus domestica cv. Golden Delicious） by bioinformatic analysis and RNA library sequencing. From these, 135 were conserved and 11 were novel miRNAs. Target analysis predicted one of the novel miRNAs, Md-miRLn11 （Malus domestica microRNA Ln11）, targeted an apple nucleotide-binding site （NBS）-Ieucine-rich repeat （LRR） class protein coding gene （Md-NBS）. 5/ RACE assay confirmed the ability of Md-miRLn11 to cleave Md-NBS at the 11-12-nt position. Analysis of the expression of Md-miRLn11 and Md-NBS during the optimum invasion period in 40 apple varieties showed that the expression of Md-NBS gene in resistant varieties is higher than in susceptible varieties, with an inverse pattern for Md-miRLn11. Seedlings from the resistant apple variety ＇JiGuan＇ were used to carry out an Agrobacterium infiltration assay, and then inoculated with the apple leaf spot disease. The result showed a clear decline of disease resistance in JiGuan apples. In contrast, the susceptible variety ＇FuJi＇ infiltrated with the Md-NBS gene showed a significant increase in disease resistance. Based on the above results, we propose that Md-miRLn11 regulates Md-NBS gene expression in particular under the condition of pathogen infection, and that the Md-miRLn11 targeting P-loop site may regulate many NBS-LRR protein class genes in woody plants.

关键词： apple microRNA NBS-LRR protein bioinformatics analysis gene function analysis

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Exponentially increased nucleation ability for poly(L-lactide) by adding acid-oxidized multiwalled carbon nanotubes with reduced aspect ratios

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Science China Chemistry 2013年第2期56卷 181-194页

作者： ZHONG Yong ZHANG YaQiong YANG JingJing LI WenLin wang zhigang XU DongHua CHEN ShouYu DING YunSheng CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and Engineering Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Institute of Polymer Materials & Chemical Engineering School of Chemical Engineering Hefei University of Technology Anhui Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering

Acid-oxidized multiwalled carbon nanotubes (A-MWCNTs) with a range of reduced aspect ratios (from about 11 to 5.8) were obtained by acid oxidization of MWCNTs in the mixture of HNO 3 and H 2 SO 4 for varying periods of 1, 3, 8 and 12 h, respec- tively. The aspect ratios and surface functionalization of A-MWCNTs were well characterized by scanning electron microsco- py (SEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and thermogravimetric analysis (TGA). Poly(L-lactide)/A-MWCNT composites containing 0.5 wt% A-MWCNTs with a range of reduced aspect ratios were prepared by solution cast. The effects of added A-MWCNTs on the isothermal crystallization kinetics of poly(L-lactide)/A-MWCNT composites were investigated by means of differential scanning calorimetry (DSC), rheology and polarized optical microscopy (POM). It is surprising to find that not only the addition of A-MWCNTs effectively increases the poly(L-lactide) (PLA) crys- tallization kinetics, but also the nucleation ability of A-MWCNTs for PLA crystallization exponentially increases with the re- duced aspect ratio, that is to say, those with lower aspect ratios show much stronger nucleation ability for PLA crystallization than those with higher aspect ratios. The exponentially increased nucleation ability of A-MWCNTs with a range of reduced aspect ratios for PLA crystallization is disclosed.

关键词： poly(L-lactide) MWCNTs aspect ratio nucleation

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Analysis of New Microsatellite Markers Developed From Reported Sequences of Japanese Flounder Paralichthys olivaceus

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Journal of Ocean University of China 2010年第4期9卷 365-370页

作者： YU Haiyang JIANG Liming CHEN Wei wang Xubo wang zhigang ZHANG Quanqi College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding Ministry of EducationOcean University of China Qingdao 266003 P. R. China

The expressed sequence tags（ESTs）of Japanese flounder,Paralichthys olivaeeus,were selected from GenBank to identify simple sequence repeats（SSRs）or microsatellites.A bioinformatic analysis of 11111 ESTs identified 751 SSR-containing ESTs,including 440 dinucleotide,254 trinucleotide,53 tetranueleotide,95 pentanucleotide and 40 hexanucleotide microsatellites *** CA/TG and GA/TC repeats were the most abundant ***-rich types were predominant among trinucleotide and tetranucleotide *** primers were designed to amplify 10 identified microsatellites *** PCR results from eight pairs of primers showed polymorphisms in wild *** 30 wild individuals,the mean observed and expected heterozygosities of these 8 polymorphic SSRs were 0.71 and 0.83 respectively and the average PIC value was *** microsatellite markers should prove to be a useful addition to the microsatellite markers that are now available for this species.

关键词： bioinformatics data mining microsatellite Japanese flounder EST

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