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Inducible nitric oxide synthase and Fas/FasL with C3 expression of mouse retinal pigment epithelial ceils in response to stimulation by peroxynitrite and antagonism of puerarin

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Chinese Medical Journal 2011年第16期124卷 2522-2529页

作者： HAO Li-na ZHANG Yan-qing SHEN Yu-hua wang Zhi-yun wang Yan-hua Department of Ophthalmology Hebei Province People's HospitalShijiazhuang Hebei 050051 China

Background Retinal pigment epithelial （RPE） cell is a monolayer of multifunctional cells between the retina and the choroid. Peroxynitrite （ONOO-） is known to induce toxicity on RPE cells. This study aimed to evaluate ONOO- induced expression of inducible nitric oxide synthase （iNOS） and complement 3 （C3） via Fas/FasL pathway in RPE cells and the values of puerarin as a therapeutic target for inhibiting the apoptosis of RPE cells. Methods RPE cells were obtained from eyes of C57BL/6 mice. RPE cells were divided into control, ONOO-and puerarin groups. Control group was treated with saline, ONOO-group was treated with ONOO-, and puerarin group was treated with puerarin after added with ONOO-. All changes were observered at 6, 12 and 24 hours after treatment. Western blotting analysis was used to determine the expression of nitrotyrosine （NT, the foot print of ONOO-） and C3; flow cytometry was used to determine the apoptosis of RPE cells. Immunohistochemistry and Western blotting were used to determine Fas/FasL signal transduction. Gene array analysis, reverse transcription polymerase chain reaction （RT-PCR） and Western blotting were used to determine the expression of iNOS mRNA and iNOS protein in RPE cells. Results There were minor expression of NT, C3, Fas/FasL and iNOS mRNA in control group, and strong expression of NT and C3 in ONOO- group, while in puerarin group weak expressions of NT and C3 were detected as time passed by （P 〈0.001）. Apoptosis of RPE cells occured and reached a higher level at 6 and 24 hours after addition of ONOO- respectively in ONOO- group, but delayed apoptosis in puerarin group （P 〈0.05）. Compared to control group, the expression of Fas/FasL was up-regulated in ONOO- group, but was down-regulated in puerarin group （P 〈0.001）. Similarly, the expressions of iNOS mRNA and iNOS protein in ONOO-group were up-regulated in ONOO- group, but down-regulated in puerarin group （P 〈0.001）. Conclusions ONOO- expresseion in RPE cells may constitute the n

关键词： retinal pigment epithelial cells oxidative cell signal complement puerarin

Effects of B, Mo, Zn, and Their Interactions on Seed Yield of Rapeseed (Brassica napus L.)

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Pedosphere 2009年第1期19卷 53-59页

作者： YANG Mei SHI Lei XU Fang-Sen LU Jian-Wei wang yun-hua Microelement Research Center Huazhong Agricultural University Wuhan 430070 （China）. National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 （China）

A field experiment was conducted to study the effects of boron (B), molybdenum (Mo), zinc (Zn) and their interactions on seed yield and yield formation of rapeseed (Brassica napus L. var. huashuang 4). Application of B fertilizer to a sandy soil increased the seed yield by 46.1% compared to the control and also created a considerably higher seed yield than the two treatments solely applying Mo and Zn fertilizers, which suggested that B was a main constraint for the seed yield of huashuang 4 in this experiment. The effect of B fertilizer on the seed yield was attributed to an increase in the number of seeds per silique and siliques per plant. The combined application of B with Mo or Zn resulted in higher seed yield than the application of B, Mo or Zn alone, and the seed yield of the B+Mo+Zn treatment was the highest in all treatments, 68.1% above the control. Dry matter accumulation of seed followed a typical S-shaped curve and it was higher in plants supplied with B than in plants without B. A small but significant increase in the seed oil content and an improvement in the oil quality were also observed in all treatments compared with the control. These results suggested that optimal micronutrient application could provide both yield and quality advantages for rapeseed in poor soil.

关键词： boron Brassica napus molybdenum seed yield zinc

Alternating spin splitting of electronic and magnon bands in two-dimensional altermagnetic materials

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Chinese Physics B 2024年第9期33卷 194-198页

作者： Qian wang Da-Wei Wu Guang-hua Guo Meng-Qiu Long yun-Peng wang School of Physics Central South UniversityChangsha 410083China

Unconventional antiferromagnetism dubbed as altermagnetism was first discovered in rutile structured magnets,which is featured by spin splitting even without the spin–orbital coupling *** interesting phenomenon has been discovered in more altermagnetic *** this work,we explore two-dimensional altermagnetic materials by studying two series of two-dimensional magnets,including MF4 with M covering all 3d and 4d transition metal elements,as well as TS2 with T=V,Cr,Mn,*** the magnetic symmetry operation of RuF4 and MnS2,it is verified that breaking the time inversion is a necessary condition for spin *** on symmetry analysis and first-principles calculations,we find that the electronic bands and magnon dispersion experience alternating spin splitting along the same *** work paves the way for exploring altermagnetism in two-dimensional materials.

关键词： two-dimensional altermagnetic materials altermagnetism spin splitting first-principles calculations

Precise determination of the pole position of the exotic Z_(c)(3900)

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Science China(Physics,Mechanics & Astronomy) 2024年第9期67卷 84-90页

作者： yun-hua Chen Meng-Lin Du Feng-Kun Guo School of Mathematics and Physics University of Science and Technology BeijingBeijing 100083China School of Physics University of Electronic Science and Technology of ChinaChengdu 611731China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Peng huanwu Collaborative Center for Research and Education Beihang UniversityBeijing 100191China

We perform a unified description of the experimental data of the π^(+)π^(-)and J/ψπ^(±)invariant mass spectra for e^(+)e^(-)→J/ψπ^(+)π^(-)and the D^(0)D^(*-)mass spectrum for e^(+)e^(-)→D^(0)D^(*-)π^(+)at e^(+)e^(-)center-of-mass energies 4.23 and 4.26 *** analysis takes into account open-charm meson loops that contain triangle singularities,the J/ψπ-DD^(*)coupled-channel interaction respecting unitarity,and the strong ππ-KK final state interaction using dispersion *** analysis leads to a precise determination of the Z_(c)(3900)pole with the pole mass and width(3880.7±1.7_(stat)±22.4_(syst))MeV and(35.9±1.4_(stat)±15.3_(syst))MeV,respectively,and hints at that the DD^(*)molecular and non-molecular components are of similar importance in the Z_(c)(3900)formation.

关键词： exotic hadrons dispersion relations final state interaction triangle singularity

Quasi-shadowing for Z^(d)-actions

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Acta Mathematica Sinica,English Series 2024年第6期40卷 1563-1580页

作者： Juan PAN Xian Kun REN yun hua ZHOU School of Mathematics and Statistics Chongqing Technology and Business UniversityChongqing 400067P.R.China College of Mathematics and Statistics Chongqing UniversityChongqing 401331P.R.China

A diffeomorphism is non-uniformly partially hyperbolic if it preserves an ergodic measure with at least one zero Lyapunov *** prove that a C^(1)-smooth Z^(d)-action has the quasishadowing property if one of the genera... 详细信息

关键词： Quasi-shadowing Z^(d)-action non-uniformly partially hyperbolic ergodic measure

Rebuilding the theory of isotope fractionation for evaporation of silicate melts under vacuum condition

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Acta Geochimica 2024年第4期43卷 661-676页

作者： Jie wang yun Liu Research Center for Planetary Science College of Earth and Planetary SciencesChengdu University of TechnologyChengdu 610059China State Key Laboratory of Ore Deposit Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China CAS Center for Excellence in Comparative Planetology Hefei 230026China

Isotope eff ects are pivotal in understanding silicate melt evaporation and planetary accretion *** on the Hertz-Knudsen equation,the current theory often fails to predict observed isotope fractionations of laboratory experiments due to its oversimplified ***,we point out that the Hertz-Knudsen-equation-based theory is incomplete for silicate melt evaporation cases and can only be used for situations where the vaporized species is identical to the one in the *** propose a new model designed for silicate melt evaporation under *** model considers multiple steps including mass transfer,chemical reaction,and *** derivations reveal a kinetic isotopic fractionation factor(KIFF orα)αour model=[m(^(1)species)/m(^(2)species)]^(0.5),where m(species)is the mass of the reactant of reaction/nucleation-limiting step or species of diffusion-limiting step and superscript 1 and 2 represent light and heavy isotopes,*** model can eff ectively reproduce most reported KIFFs of laboratory experiments for various elements,i.e.,Mg,Si,K,Rb,Fe,Ca,and ***,the KIFF-mixing model referring that an overall rate of evaporation can be determined by two steps jointly can account for the eff ects of low P_(H2)pressure,composition,and *** addition,we find that chemical reactions,diffusion,and nucleation can control the overall rate of evaporation of silicate melts by using the fitting slope in ln(−ln f)versus ln(t).Notably,our model allows for the theoretical calculations of parameters like activation energy(E_(a)),providing a novel approach to studying compositional and environmental eff ects on evaporation processes,and shedding light on the formation and evolution of the proto-solar and Earth-Moon systems.

关键词： Evaporation Kinetic isotopic fractionation Chemical kinetics Hertz-Knudsen equation CAIS

Study on the Nutrition Characteristics of Different K Use Efficiency Cotton Genotypes to K Deficiency Stress

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Agricultural Sciences in China 2008年第6期7卷 740-745页

作者： JIANG Cun-cang CHEN Fang GAO Xiang-zhao LU Jian-wei WAN Kai-yuan NIAN Fu-zhao wang yun-hua Plant Nutrition Lab Huazhong Agricultural University Wuhan 430070 P.R.China Wuhan Botanical Garden Chinese Academy of Sciences Wuhan 430074 P.R.China National Agro-Tech Extension and Service Center Beijing 100026 P.R.China

To study the mechanism of plant K use efficiency, both K high and low use efficiency cotton genotypes, 103 and 122, respectively, were selected from 86 cotton cultivars （Gossypium hirsutum L.）. The research was conducted using pot experiment for planting cotton on without K （0.0 g kg-1 soil） and with K （0.4 g kg-1 soil） treatments in 2005. Experimental result showed that, with K deficiency stress, genotype 103 grew much better than genotype 122 except that its lower leaves showed the symptoms of K deficiency, whereas all the leaves of genotype 122 showed the symptoms of K deficiency. Root dry matter weights of treatments for genotype 103 with and without K application were 1.07 and 1.25 times of genotype 122. It indicated that the root system of genotype 103 was well developed and has better nutrition uptake capability than that of genotype 122. The result also showed that the cotton shoots of genotype 103 were 1.07 and 1.13 times over genotype 122 on treatments of with and without K application. It indicated that genotype 103 has stronger transport organs. In genotype 103, plants, dry matter, and potassium were mainly transported to cotton bolls. The boll dry weight of genotype 103 was 2.58 times in without K treatment and 1.90 times in with K treatment over genotype 122. The potassium accumulation in bolls of genotype 122 was only 49.3% of that in genotype 103. Potassium accumulation in the other organs of genotype 103 was relatively low compared with in bolls. This indicated that the distribution of K and organic matter in genotype 103 was more efficient than genotype 122. The main differences between high K efficiency cotton genotype 103 and low K efficiency genotype 122 lie in their potassium nutrition and organic matter using efficiency on uptake, transportation, accumulation, distribution, and utilization.

关键词： cotton (Gossypium hirsutum L.) potassium use efficiency genotype uptake capability distribution ability

Hydrophobic long-chain two-dimensional perovskite scintillators for underwater X-ray imaging

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Rare Metals 2024年第1期43卷 175-185页

作者： Jin-Xiao Zheng Zi-An Zhou Tiao Feng Hui Li Cheng-hua Sun Nüwang Yang Tian Yong Zhao Shu-yun Zhou Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Bioinspired Smart Interfacial Science and Technology of the Ministry of Education Beijing Key Laboratory of Bioinspired Energy Materials and DevicesSchool of ChemistryBeijing Advanced Innovation Center for Biomedical EngineeringBeihang UniversityBeijing 100191China Department of Engineering Physics Tsinghua University and Key Laboratory of Particle and Radiation Imaging Ministry of EducationTsinghua UniversityBeijing 100084China e-mail:yangt@***

The underwater X-ray imaging technology development is significant to subaqueous target reconnaissance/detection/identification, subfluvial archaeology,submerged resource exploration, etc. As the core of X-ray imaging detection, the scintillator has been plagued by inherent moisture absorption and decomposition, and strict requirements for seamless packaging and ***, we designed a manganese-doped two-dimensional(2D) perovskite scintillator modified by hydrophobic longchain organic amine through the combination of component and doping engineering. The modified perovskites show high water repellency that can be used as an underwater X-ray scintillator. X-ray images of aquatic organisms or other objects with a high spatial resolution of10 lp·mm^(-1) at a big view field(32 mm × 32 mm) were obtained by scintillation screen. This hydrophobic perovskite scintillator based on molecular design is of great promise in underwater X-ray nondestructive testing technology development.

关键词： Two-dimensional perovskite Hydrophobic Scintillators Underwater X-ray imaging Underwater nondestructive testing technology

Quantized bound states around a vortex in anisotropic superconductors

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Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 146-155页

作者： Ke Xiang Da wang Qiang-hua wang National Laboratory of Solid State Microstructures&School of Physics Nanjing UniversityNanjing 210093China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China

The bound states around a vortex in anisotropic superconductors is a longstanding yet important *** this work,we develop a variational theory on the basis of the Andreev approximation to obtain the energy levels and wave functions of the low-energy quantized bound states in superconductors with anisotropic pairing on arbitrary Fermi *** the case of circular Fermi surface,the effective Schr¨odinger equation yielding the bound state energies gets back to the theory proposed by Volovik and Kopnin many years *** generalization here enables us to prove the equidistant energy spectrum inside a vortex in a broader class of *** importantly,we are now able to obtain the wave functions of these bound states by projecting the quasiclassical wave function on the eigenmodes of the effective Schr¨odinger equation,going beyond the quasiclassical Eilenberger results,which,as we find,are sensitive to the scattering *** the case of isotropic Fermi surface,the spatial profile of the low-energy local density of states is dominated near the vortex center and elongates along the gap antinode directions,in addition to the ubiquitous Friedel oscillation arising from the quantum inteference neglected in the Eilenberger ***,as a consequence of the pairing anisotropy,the quantized wave functions develop a peculiar distribution of winding number,which reduces stepwise towards the vortex *** work provides a flexible way to study the vortex bound states in the future.

关键词： vortex bound state anisotropic superconductor Andreev approximation