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Influences of Mo on Nitrate Reductase, Glutamine Synthetase and Nitrogen Accumulation and Utilization in Mo-Efficient and Mo-Inefficient Winter Wheat Cultivars

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Agricultural Sciences in China 2010年第3期9卷 355-361页

作者： YU Min HU Cheng-xiao SUN Xue-cheng wang yun-hua Research Center of Trace Elements Huazhong Agricultural University Wuhan 430070 P.R. China Department of Horticulture Foshan University Foshan 528000 P.R. China

The objective is to study whether the accumulation and utilization of plant N are controlled by Mo status in winter wheat cultivars. Mo-efficient cultivar 97003 （eft） and Mo-inefficient cultivar 97014 （ineff） were grown in severely Mo-deficient acidic soil （Tamm-reagent-extractable Mo 0.112 mg kg^-1） with （＋Mo） and without （-Mo） the application of 0.13 mg kg^-1 Mo. The accumulation and use efficiency of plant total N were significantly higher in ＋Mo than that in -Mo and in eft than that in ineff under Mo deficiency. N use efficiency was remarkably higher in maturity but it was forwarded to jointing stage after Mo supply, thus indicating that Mo supply promoted the N use efficiency besides N uptake and eff was efficient in N uptake and utilization. The overall activity of nitrate reductase （NR, EC 1.***.6.1） was significantly higher in ＋Mo than in -Mo and ratio of ＋Mo/-Mo was even to 14.8 at filleting stage for ineff. Activity of glutamine synthetase （GS, EC 6.***.1.2） was significantly lower in ＋Mo than in -Mo. Concentration of nitrate and glutamate were also significantly lower in ＋Mo than in -Mo, thus provided evidences for enhancing N use efficiency by Mo supply. Activities of NR and GS were significantly higher and concentrations of nitrate and glutamate were significantly lower in eff than ineff under Mo deficiency, thus indicated eff was more efficient in N reduction and utilization. It is therefore concluded that Mo could promote N accumulation and utilization in winter wheat which was directly related to NR and feedback regulated by GS. Higher Mo status also results in higher accumulation and utilization of plant N in eft.

关键词： winter wheat Mo N accumulation N use efficiency nitrate reductase glutamine synthetase

Effects of Molybdenum on the Intermediates of Chlorophyll Biosynthesis in Winter Wheat Cultivars Under Low Temperature

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Agricultural Sciences in China 2006年第9期5卷 670-677页

作者： YU Min HU Cheng-xiao wang yun-hua Research Center of Trace Elements Huazhong Agricultural University Wuhan 430070 P.R.China Department of Horticulture Foshan University Foshan 528000 P.R.China

The objective was to probe the site where the biosynthesis of chlorophyll was blocked under Mo deficiency at low temperature, which led to the decrease of chlorophyll in winter wheat cultivars. The intermediates of chlorophyll biosynthesis were analyzed in winter wheat cultivars in soil culture, miniblock culture, and solution culture to study the effects of Mo on chlorophyll biosynthesis without Mo addition （CK, soil available Mo 0.112 mg kg^-1） and Mo addition （＋ Mo, 0.13 mg kg^-1 Mo was added）. Laevulinic acid （LA）, the competitive analog of δ-aminolaevulinic acid （ALA） was also introduced in the experiment. The ratio of Chl a/Chl b was constant between CK and ＋ Mo treatment, whereas it increased at low temperature, which indicated that Mo deficiency did not inhibit the transformation of Chl a to Chl b at low temperature. Under Mo deficiency, the contents of protochlorophyll （Pchl）, Mg-protoporphyrin Ⅸ （Mg-Proto Ⅸ）, protoporphyrin Ⅸ （proto IX）, and uroporphyrinogen Ⅲ （Uro Ⅲ） decreased [Uro Ⅲ decreased significantly （P 〈0.01）], whereas ALA and glutamate increased significantly （P 〈 0.01） compared with that of Mo addition, which suggested that the transformation from ALA to Uro Ⅲ might be inhibited. The content of ALA reversed after addition of LA, it was significantly higher （P 〈 0.01） in Mo addition than in CK. The results indicated that the transformation from ALA to Uro Ⅲ was blocked under Mo deficiency, which resulted in the inhibition of the biosynthesis of chlorophyll and led to the decrease of chlorophyll in winter wheat cultivars.

关键词： chlorophyll δ-aminolaevulinic acid uroporphyrinogen Ⅲ glutamate laevulinic acid winter wheat cultivars

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一种新型电流源型APF及其控制

电力电子技术 2024年第6期58卷 93-98页

作者：何芳刘斌黄嘉文王华云南昌航空大学信息工程学院江西南昌330063 国网江西省电力有限公司电力科学研究院江西南昌330077

电压源型有源电力滤波器(APF)常采用三相全桥结构,这种结构存在开关损耗大,至少需要3个功率电感,系统体积大等不足。此处提出了一种新型电流源型APF结构,该结构在一个PWM周期之内只使用3个高频开关和一个主电感。相较于传统电压源型APF... 详细信息

电压源型有源电力滤波器(APF)常采用三相全桥结构,这种结构存在开关损耗大,至少需要3个功率电感,系统体积大等不足。此处提出了一种新型电流源型APF结构,该结构在一个PWM周期之内只使用3个高频开关和一个主电感。相较于传统电压源型APF,所提结构在提升效率和降低成本等方面更有优势。根据不同的电压及电流状态,此处对系统的开关模式进行了讨论,并对其中典型模式的换流状态进行了分析,在此基础上说明了系统的调制及控制策略,对有源滤波时的电感电流给定和前馈占空比等情况进行了分析,并进一步讨论了系统的硬件设计。最后通过仿真和实验对所提结构的有效性进行验证。

关键词：电流源型有源电力滤波器效率开关模式前馈占空比

Plant Growth,Yield Components,Economic Responses,and Soil Indigenous K Uptake of Two Cotton Genotypes with Different K-Efficiencies

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Agricultural Sciences in China 2011年第5期10卷 705-713页

作者： JIANG Cun-cang XIA Ying CHEN Fang LU Jian-wei wang yun-hua College of Resources and Environment Huazhong Agricultural University Wuhan 430070 P.R.China Wuhan Botanical Garden Chinese Academy of Sciences Wuhan 430074 P.R.China

HG103,a high K-efficiency cotton cultivar with high-yield potential,and LG122,a low K-efficiency cotton cultivar with low-yield potential were used to study the genotypic variation on yield,economic responses,and soil indigenous K exploitation for cotton in pot and field conditions in 2006 and *** indicated that both the cultivars differed significantly in the rate of translocation of dry matter into reproductive organs and the time of running into reproductive *** yield and economic parameters of HG103 were much better than *** content in cotton shells reached the highest level at maturity stage for both *** also showed that the K content in each plant part of HG103 was lower than that of *** two genotypes showed different efficiencies and abilities on absorbing soil rapidly available K and slowly available ***122 showed higher absorption of K rate than HG103 after K fertilization,but on the contrary,HG103 uptake more soil indigenous K than LG122 in no K fertilization *** result indicated that HG103 could tolerant and absorb more soil indigenous K than LG122,under condition of soil K ***122 could grow well and absorb more K nutrient when soil K supply is *** results demonstrated that higher K content in plant tissues and higher K uptake by plant may not an exclusive condition for high yield planting with K *** translocation of more dry matters into reproductive organs is one of the key mechanisms of high K use efficiency in cotton.

关键词： cotton (Gossypium hirsutum L.) dry matter partitioning K depletion indigenous K uptake economic parameter

Inheritance of Boron Efficiency in Oilseed Rape

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Pedosphere 2009年第3期19卷 403-408页

作者： SHI Lei wang yun-hua NIAN Fu-Zhao LU Jian-Wei MENG Jin-Ling XU Fang-Sen National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research （Wuhan） Huazhong Agriculture University Wuhan 430070 （China） Microelement Research Centre College of Resources and Environment Huazhong Agriculture University Wuhan 430070 （China）

Field experiments were conducted to study the inheritance of boron efficiency in oilseed rape (Brassica napus L.) by evaluating the boron (B) efficiency coefficient (BEC, the ratio of the seed yield at below the critical boron level to that at the boron-sufficient level) with 657 F2:3 lines of a population derived from a cross between a B-efficient cultivar, Qingyou 10, and a B-inefficient cultivar, Bakow. Qingyou 10 had high BEC as well as high seed yield at low available soil B. On the contrary, Bakow produced low seed yield at low B status. Boron deficiency decreased the seed yield of the F2:3 lines to different extents and the distribution of BEC of the population showed a bimodal pattern. When the 657 F2:3 lines were grouped into B-efficient lines and B-inefficient lines according to their BEC, the ratio of B-efficient lines to B-inefficient lines fitted the expected ratio (3:1), indicating that one major gene controlled the B-efficiency trait. 127 F2:3 lines selected from the population at random, with distribution of BEC similar to that of the overall population, were used to identify the target region for fine mapping of the boron efficiency gene.

关键词： boron efficiency boron level Brassica napus L. inheritance seed yield

HPLC双波长法测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量

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中国兽药杂志 2024年第4期58卷 43-49页

作者：石慧慧刘建晖熊玥周杨汪云花孙瑶江苏省畜产品质量检验测试中心南京210036

建立HPLC双波长法同时测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量。采用XBridge C_(18)色谱柱(4.6 mm×150 mm,5μm),以甲醇-水(52∶48)为流动相,流速1.0 mL·min^(-1);柱温25℃;进样量10μL,检测波长分别为225 nm(穿心莲内酯... 详细信息

建立HPLC双波长法同时测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量。采用XBridge C_(18)色谱柱(4.6 mm×150 mm,5μm),以甲醇-水(52∶48)为流动相,流速1.0 mL·min^(-1);柱温25℃;进样量10μL,检测波长分别为225 nm(穿心莲内酯)和254 nm(脱水穿心莲内酯)。穿心莲内酯浓度在12.7375μg·mL^(-1)~509.5μg·mL^(-1)范围内,线性良好(r^(2)=0.9996);脱水穿心莲内酯浓度在3.4μg·mL^(-1)~136μg·mL^(-1)范围内,线性关系良好(r^(2)=0.9997)。穿心莲内酯加样回收率平均为98.8%,RSD为1.0%;脱水穿心莲内酯回收率平均为99.9%,RSD为0􀆰8%。该方法快捷、灵敏、准确、可靠,可用于四味穿心莲散中穿心莲内酯和脱水穿心莲内酯2个有效成分的同步测定,为四味穿心莲散的质量控制和评价提供科学依据。

关键词： HPLC双波长法四味穿心莲散穿心莲内酯脱水穿心莲内酯

小切口复位临时固定辅助股骨近端防旋髓内钉治疗股骨转子间骨折

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临床骨科杂志 2024年第1期27卷 83-86页

作者：王浩然张文杰郑宏瑞何斌王云华范磊南京医科大学第二附属医院骨科江苏南京210011

目的探讨小切口复位临时固定辅助股骨近端防旋髓内钉(PFNA)治疗股骨转子间骨折的疗效。方法采用小切口复位临时固定辅助PFNA治疗28例股骨转子间骨折患者。记录术中出血量、手术时间、骨折复位质量、骨折愈合情况。采用Harris评分评价髋... 详细信息

目的探讨小切口复位临时固定辅助股骨近端防旋髓内钉(PFNA)治疗股骨转子间骨折的疗效。方法采用小切口复位临时固定辅助PFNA治疗28例股骨转子间骨折患者。记录术中出血量、手术时间、骨折复位质量、骨折愈合情况。采用Harris评分评价髋关节功能。结果患者均获得6个月随访。术后无切口感染情况发生。术中出血量95~110(100.5±8.0)ml,手术时间45~57(50.5±4.5)min。骨折复位质量:解剖复位20例,功能复位5例,复位不良3例。术后3个月Harris评分67~95(88.32±8.07)分,其中优20例、良5例、差3例,优良率25/28。26例骨折愈合,时间3~4个月,其中1例术后髋内翻畸形,无螺钉松动,应患者要求未做特殊处理;1例术后螺旋刀片向外侧切出、1例术后骨折不愈合,考虑二次行翻修手术治疗。结论小切口复位临时固定辅助PFNA治疗股骨转子间骨折,手术操作简单,创伤小,不影响PFNA操作,可辅助增强牵引床的牵引作用,疗效满意。

关键词：股骨转子间骨折内固定顶棒克氏针复位钳股骨近端防旋髓内钉

Propagation and Dispersion Characteristics of Scholte Wave in VTI Media with the Presence of Irregular Seabed

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Journal of Earth Science 2024年第2期35卷 722-725页

作者： Xiaobo Liu yun wang Jianlei Zhang Haifeng Chen hua Zhang School of Ocean Sciences China University of GeosciencesBeijing 100083China Key Laboratory of Polar Geology and Marine Mineral Resources China University of GeosciencesBeijing 100083China School of Geophysics and Information Technology China University of GeosciencesBeijing 100083China Research&Development Center BGP IncCNPCZhuozhou 072751China National Engineering Research Center for Oil and Gas Exploration Computer Software Beijing 100080China

INTRODUCTION Surface waves propagating along the seafloor are general ly called Scholte waves,and were first discovered and studied in the early 1950s(Kugler et al.,2005;Buchen and Ben-Hador,1996).Scholte waves exhibit a dispersion phenomenon,which implies that their velocity varies with ***-frequency Scholte waves can propagate over long distances on the seabed with little attenuation(Bohlen et al.,2004).The particle mo tion of Scholte waves in a solid medium changes from a retro grade to a prograde ellipse(Klein et al.,2005;McMechan and Yedlin,1981).

关键词： seabed Dispersion implies

High-resolution remote thermometry based on Bi^(3+)-doped 0D zinc halide hybrids

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Science China Chemistry 2024年第8期67卷 2599-2605页

作者： Cui-Mi Shi Shu-hua Xue Jin-yun wang Liang-Jin Xu Zhong-Ning Chen State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China Fujian Science Technology Innovation Laboratory for Optoelectronic Information of ChinaFuzhou 350108China University of Chinese Academy of Sciences Beijing 100049China

Developing highly sensitive optical thermometers is of great significance due to their capability to enable remote and non-contact temperature measurements, rendering them highly applicable in diverse and harsh environments. Herein we report a temperature-dependent phosphor of 0D metal halide hybrid by incorporating Bi^(3+)into the(MePPh_(3))_(2)ZnCl_(4) matrix. Through Bi^(3+)doping,the initially non-luminescent(MePPh_(3))_(2) ZnCl_(4) matrix exhibits a deep-blue emission centered at 453 nm, with a photoluminescence quantum yield(PLQY) of 5.71% and a Stokes shift of 75 nm at room temperature. Experimental characterization demonstrates that exciton-like luminescence of Bi^(3+)is mainly responsible for the blue emission. Single crystals of Bi^(3+)-doped(MePPh_(3))_(2)ZnCl_(4) show an unusual correlation between photoluminescence(PL) lifetime and temperature. Particularly, the dependence of luminescence lifetime on temperature is most remarkable in the temperature range of 80 to 100 K with an exceptional sensitivity up to 0.09 K^(-1), representing one of the best levels for thermometry based on PL decay lifetime. Our work not only provides a viable strategy for designing a novel, environmentally friendly, and stable blue emitter, but also paves the way for precise thermometric application at low temperature.

关键词： lead-free hybrids Bi^(3+)doping blue emission temperature sensing