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Et_3NHCl-AlCl_3 Ionic Liquids as Catalyst for Alkylation of Toluene with 2-Chloro-2-methylpropane

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China Petroleum Processing & Petrochemical Technology 2013年第1期15卷 54-60页

作者： Chen Han Luo Guohua Xu Xin wang yanli Xia Jiajia Department of Chemical Engineering Beijing Institute of Petrochemical Technology College of Chemical Engineering Beijing University of Chemical Technology

Alkylation of toluene With 2-chloro-2-methylpropane （t-Bu-C1） to synthesize para-tert-butyltoluene （PTBT） was carded out in the presence of triethylamine hydrochloride-aluminum chloride ionic liquids used as the catalyst. The ionic liquids were prepared with different molar ratios of Et3NHC1 to A1CI3, and the effect of the molar ratio between A1C13 and Et3NHC1, the reaction time, the reaction temperature, the ionic liquid dosage, as well as the molar ratio of toluene to chloro- 2-methylpropane on the alkylation reaction of toluene with chloro-2-methyl-propane was investigated. The test results showed that the acidic ionic liquids prepared with Et3NHC1 and A1C13 had good activity and selectivity for the alkylation reaction of toluene with alkyl chloride to produce PTBT. The optimal reaction conditions were specified at an A1C13 to Et3N- HCI ratio of 1.6, a reaction temperature of 20 ℃, a mass fraction of toluene to ionic liquid of 10%, and a chloro-2-methyl- propane to toluene molar ratio of 0.5. Under the suitable reaction conditions, a 98% conversion of chloro-2-methylpropane and an 82.5% selectivity of PTBT were obtained. Ionic liquids could be reused 5 times with its catalytic activity unchanged, and the regenerated ionic liquids can be recycled.

关键词： alkylation ionic liquid toluene 2-chloro-2-methylpropane EhNHC1-A1C13

姜黄素抑制NF-κB核易位调控胰腺癌对吉西他滨的敏感性

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重庆医学 2024年第15期53卷 2247-2253页

作者：王艳丽戈立秀曾昭伟王睿张爱民天津市南开医院检验科天津300100 天津市中西医结合急腹症研究所天津300100 天津金域医学检验实验室有限公司天津300100

目的探讨姜黄素调控胰腺癌细胞增强吉西他滨敏感性的机制。方法为验证姜黄素对胰腺癌PANC1细胞中p53及核因子-κB(NF-κB)p65核易位的影响,将细胞以0、10、20、30μmol/L姜黄素处理,得到空白组、10μmol/L姜黄素组、20μmol/L姜黄素组... 详细信息

目的探讨姜黄素调控胰腺癌细胞增强吉西他滨敏感性的机制。方法为验证姜黄素对胰腺癌PANC1细胞中p53及核因子-κB(NF-κB)p65核易位的影响,将细胞以0、10、20、30μmol/L姜黄素处理,得到空白组、10μmol/L姜黄素组、20μmol/L姜黄素组和30μmol/L姜黄素组;采用免疫荧光染色技术在荧光显微镜下确定NF-κB p65细胞内分布情况;Western blot技术确定细胞内p53蛋白的表达水平;逆转录-实时荧光定量PCR技术确定细胞内p53 mRNA的表达水平;联合采用miRstar和JASPAR软件预测寻找可能受NF-κB p65核转位调控的微RNA(miRNA);采用双荧光素酶报告实验分别验证miRNA与p53的关系。为验证姜黄素是否通过NF-κB p65/miR-26b-5p/p53轴影响吉西他滨对PANC1细胞的敏感性,以10μmol/L的吉西他滨处理细胞得到吉西他滨组,以20μmol/L姜黄素及10μmol/L吉西他滨处理细胞得到姜黄素联合吉西他滨组;经姜黄素(20μmol/L)和吉西他滨(10μmol/L)联合处理细胞后,分别将寡核苷酸对照(mimic NC)和miR-26b-5p模拟物(mimic)转染至细胞,得到mimic NC和mimic miR-26b-5p组;将pcDNA3.1空载质粒和pcDNA 3.1-p53过表达质粒分别转染至细胞,得到pcDNA3.1组和p53组;采用MTT实验检测细胞活力;采用膜联蛋白-V(Annexin V)/碘化丙啶(PI)双染色流式细胞术测定细胞凋亡水平。结果与空白组比较,各姜黄素处理组(10、20、30μmol/L姜黄素组)细胞内p53 mRNA及蛋白表达水平均升高;与空白组比较,20μmol/L姜黄素组细胞核内NF-κB p65表达水平降低;miRstar和JASPAR预测软件找到8个miRNA可能受NF-κB p65核易位调控,其中有3个具有靶向p53基因的潜力,尤其以miR-26b-5p效果最明显。双荧光素酶报告实验验证发现miR-26b-5p与p53确有相互作用。与mimic NC组比较,mimic miR-26b-5p组p53 mRNA和蛋白表达水平均下降;与吉西他滨组比较,姜黄素联合吉西他滨组细胞活力降低、细胞凋亡率增加;与mimic NC组比较,mimic miR-26b-5p组细胞活力升高、细胞凋亡率下降,且与pcDNA 3.1组比较,pcDNA3.1-p53组细胞活力下降、细胞凋亡率升高。结论姜黄素通过抑制NF-κB p65蛋白核转位降低miR-26b-5p基因的表达,进而增加p53基因和蛋白的表达,通过NF-κB p65/miR-26b-5p/p53轴增强了胰腺癌细胞对吉西他滨的敏感性。

关键词：姜黄素 NF-κB p65/miR-26b-5p/p53轴胰腺癌吉西他滨敏感性

Folding Behaviors of Purine Riboswitch Aptamers

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Wuhan University Journal of Natural Sciences 2018年第1期23卷 43-50页

作者： GONG Sha wang yanli wang Zhen SUN Yuying ZHANG Wenbing Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization Huanggang Normal University Huanggang 438000 Hubei China School of Physics and Technology Wuhan UniversityWuhan 430072 Hubei China

Most riboswitches are characterized by two components, an aptamer domain that folds into a unique ligand binding pocket to interact with the ligand, and an expression platform that converts folding changes in the aptamer into changes in gene expression. Using the recently developed systematic helix-based computational method, we theoretically studied the refolding and co-transcriptional folding behaviors of the purine riboswitch aptamers from Bacillus subtilis xpt-pbu X guanine riboswitch and Vibrio vulnificus add adenine riboswitch. Despite several intermediate structures persisting a short time during the transcription, helices P2, P3 and P1 fold in turn for both aptamers. Although some misfolded structures are observed during the refolding process, the RNAs can fold into the ligand binding pocket structure containing helices P2, P3 and P1 within a few seconds, suggesting the aptamer domains are highly evolved. The purine riboswitch aptamers can quickly fold into the ligand binding pocket structure even at a high transcription speed, possibly because formation of this structure is the necessary prerequisite for the riboswitch to bind its ligand and then regulate relevant gene expression.

关键词： purine riboswitch gene regulation aptamer co-transcriptional folding

Effect of Preparation Process on Compressive Strength and Hydrogenation Performance of Raney-Ni/Al2O3 Catalyst

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China Petroleum Processing & Petrochemical Technology 2017年第2期19卷 14-20页

作者： Luo Guohua wang yanli Sun Daming Xu Xin Jin Haibo College of Chemical Engineering Beijing Institute of Petrochemical Technology Beijing 102617 Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology Beijing 102617 Key Laboratory of Fine Chemicals School of Chemical EngineeringDalian University of Technology Dalian 116024

In this study, Raney-Ni/Al_2O_3 catalysts were prepared from precursors which were calcined in different atmosphere(argon and air) and leached for different time. XRD, SEM, H_2-TPR and BET techniques were introduced to characterize the catalysts, and hydrogenation of unsaturated hydrocarbons, including vinyltoluene, dicyclopentadiene and indene, was used as probe reaction to evaluate the catalytic hydrogenation performance of the catalysts. The results demonstrated that the calcination atmosphere had noticeable effects on the compressive strength and hydrogenation performance of Raney-Ni/Al_2O_3. The catalyst calcined in argon exhibited better compressive strength and catalytic performance than the one calcined in air. The compressive strength and activity stability of catalyst were also determined by the leaching time. A too longer leaching time was not conducive to improving the compressive strength and catalytic performance of catalysts. When the precursor calcined in argon was treated in a 15%(by wt.) sodium hydroxide solution at 343 K for 5 h, a catalyst with higher compressive strength(at 26.10 N/mm) was obtained and the conversion of vinyltoluene, dicyclopentadiene(DCPD), and indene could amount to more than 87.5%, 99% and even 100%, respectively, under the reaction conditions covering a temperature of 493 K, a hydrogen pressure of 2.0 MPa, a LSHV of 3.0 h^(-1), and a hydrogen/oil ratio of 200:1.

关键词： nickel-aluminum alloy pseudo-boehmite unsaturated hydrocarbon hydrogenation

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占位法制备球形孔泡沫铝的结构与性能研究

轻金属 2024年第2期 49-56页

作者：闫彬王录才游晓红王芳王艳丽武建国太原科技大学材料科学与工程学院山西太原030024

以氯化钙、硬脂酸、硫代硫酸钠、尿素作为造孔剂,利用占位法进行孔径为1~2 mm,孔隙率为60%、70%、80%球形孔泡沫铝的制备。对其孔结构(孔面积、孔圆形度)、压缩性能和吸能效果进行对比分析。结果表明:氯化钙-泡沫铝因其具备较好的孔结构... 详细信息

以氯化钙、硬脂酸、硫代硫酸钠、尿素作为造孔剂,利用占位法进行孔径为1~2 mm,孔隙率为60%、70%、80%球形孔泡沫铝的制备。对其孔结构(孔面积、孔圆形度)、压缩性能和吸能效果进行对比分析。结果表明:氯化钙-泡沫铝因其具备较好的孔结构,所表现出的压缩性能和吸能效果也更优异。

关键词：造孔剂球形孔泡沫铝孔结构力学性能氯化钙

Coarsening behavior of γ' particles in a nickel-base superalloy

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Rare Metals 2009年第2期28卷 197-201页

作者： Li, Hongyu Zuo, Lingli Song, Xiping wang, yanli Chen, Guoliang State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing 100083 China

The coarsening behavior of γ particles in a nickel-base superalloy FGH95 was investigated by means of experimental observations and growth kinetics calculations. The results show that when aging at 1000,1080 and 1140°C for different times,the relation of average particle size to time obeys the cube law ( a /2)3= kt,where k is 15.49 × 103,77.5 × 103 and 230.04 × 103 nm3/min,respectively. The particle size distributions are better fit to the LSW theoretical distributions when aging at 1000°C within 1440 min....

关键词： nickel-base superalloys γ particles coarsening behavior particle size distribution activation energy

Interface engineering of MXene-based heterostructures for lithiumsulfur batteries

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Nano Research 2023年第7期16卷 9158-9178页

作者： Siyu Wu Xiang Li Yongzheng Zhang Qinghua Guan Jian wang Chunyin Shen Hongzhen Lin Jitong wang yanli wang Liang Zhan Licheng Ling State Key Laboratory of Chemical Engineering East China University of Science and Technology130 Meilong RoadShanghai 200237China Key Laboratory of Specially Functional Polymeric Materials and Related Technology(Ministry of Education) School of Chemical EngineeringEast China University of Science and Technology130 Meilong RoadShanghai 200237China i-Lab&CAS Key Laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-tech and Nano-bionicsChinese Academy of SciencesSuzhou 215123China Helmholtz Institute Ulm(HIU) Ulm D89081Germany

High energy density and low cost make lithium-sulfur(Li-S)batteries as one of the next generation's promising energy storage ***,the following problems need to be solved before commercialization:(i)the shuttling effect and sluggish redox kinetics of lithium polysulfides in sulfur cathode;(ii)the formation of lithium dendrites and the crack of solid electrolyte interphase;(iii)the large volume changes during charge and discharge ***,as newly emerging two-dimensional transition metal carbides/nitrides/carbonitrides,have attracted widespread attention due to their abundant active surface terminals,adjustable vacancies,and high electrical *** MXene-based heterogeneous structures is expected to solve the stacking problem induced by hydrogen bonds or Van der Waals force and to provide other charming physiochemical ***,we generalize the design principles of MXene-based heterostructures and their functions,i.e.,adsorption and catalysis in advanced conversion-based Li-S ***,the physiochemical properties of MXene and MXene-based heterostructures are briefly ***,the catalytic functions of MXene-based heterostructures with the compositional constituents including carbon materials,metal compounds,organic frameworks,polymers,single atoms and special high-entropy MXenes are comprehensively summarized in sulfur cathodes and lithium ***,the challenges of MXene-based heterostructure in current Li-S batteries are pointed out and we also provide some enlightenments for future developments in high-energy-density Li-S batteries.

关键词： MXene heterostructures lithium-sulfur battery shuttle effect interface engineering

盐酸伊伐布雷定对阿霉素诱导的心力衰竭大鼠心室重构的影响

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中国医药科学 2024年第18期14卷 4-9页

作者：王方泽王韵菡赵云波张瑞王艳丽马长虹王健山东省潍坊市人民医院心血管内科山东潍坊261000 山东第二医科大学公共卫生学院山东潍坊261000 山东省潍坊市第二人民医院护理部山东潍坊261000

目的探讨盐酸伊伐布雷定对阿霉素诱导的心力衰竭大鼠心室重构的影响。方法50只无特定病原体级9周龄Wistar大鼠[体重(300±10)g,雌雄不限],按照随机数表法分为对照组(10只)、模型组(20只)、治疗组(20只)。采用阿霉素诱导法构建心力衰竭模... 详细信息

目的探讨盐酸伊伐布雷定对阿霉素诱导的心力衰竭大鼠心室重构的影响。方法50只无特定病原体级9周龄Wistar大鼠[体重(300±10)g,雌雄不限],按照随机数表法分为对照组(10只)、模型组(20只)、治疗组(20只)。采用阿霉素诱导法构建心力衰竭模型,以左室射血分数(LVEF)35%~50%为建模成功的标准。治疗组盐酸伊伐布雷定[10 mg/(kg·d)]灌胃,对照组和模型组等量生理盐水灌胃,4周后,心脏超声记录左室收缩末期内径(LVEDs)、左室舒张末期内径(LVEDd)、LVEF、左室短轴缩短率(LVFS)变化,苏木精-伊红染色观察左室心肌细胞,Luminex、蛋白免疫印迹分析、酶联免疫吸附试验检测单核细胞趋化蛋白1(MCP-1)、肿瘤坏死因子-α(TNF-α)、脑钠肽(BNP)、白细胞介素-1β(IL-1β)、粒细胞集落刺激因子(G-CSF)等因子水平。结果与对照组比较,模型组LVEDs、LVEDd增加(P<0.05),LVEF、LVFS降低(P<0.05);MCP-1、TNF-α、IL-1β、G-CSF、BNP等因子水平升高(P<0.05)。与模型组比较,治疗组LVEDs、LVEDd降低(P<0.05),LVEF、LVFS增加(P<0.05),且上述因子表达水平降低(P<0.05)。对照组心肌细胞形态正常,模型组心肌细胞明显异常并伴有炎症细胞浸润,治疗后得到部分缓解。结论腹腔注射阿霉素的方法制备大鼠心力衰竭模型成功率高且稳定,盐酸伊伐布雷定可以有效抑制该模型的炎症反应,改善心室重构。

关键词：心力衰竭盐酸伊伐布雷定心室重构炎症

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Fasudil alleviates LPS-induced lung injury by restoring aquaporin 5 expression and inhibiting inflammation in lungs

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The Journal of Biomedical Research 2019年第3期33卷 156-163页

作者： Jingjing wang Hui Kong Jian Xu yanli wang Hong wang Weiping Xie Department of Respiratory & Critical Care Medicine the First Affiliated Hospital of Nanjing Medical University

Fasudil, a selective rho kinase(ROCK) inhibitor, has been reported to play a beneficial role in systemic inflammation in acute lung injury, but its mechanism for ameliorating pulmonary edema and inflammation remains unclear. Using hematoxylin-and-eosin(H&E) staining, immunohistochemistry, enzyme-linked immunosorbent assay,quantitative real time PCR and Western blotting, we found that fasudil attenuated LPS-induced lung injury, decreased lung edema, and suppressed inflammatory responses including leukocyte infiltration and IL-6 production. Further,fasudil upregulated LPS-induced aquaporin 5 reduction and inhibited NF-κB activation in the lungs of mice. Our results suggest that fasudil could restore the expression of aquaporin 5 to eliminate LPS-induced lung edema and prevent LPS-induced pulmonary inflammation by blocking the inflammatory pathway. Collectively, blockade of the ROCK pathway by fasudil may be a potential strategy for the treatment of acute lung injury.

关键词： acute lung injury aquaporin 5 fasudil fliud transport NF-κB