检索结果-南通市图书馆

A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第7期11卷 1212-1215页

作者： HU Zhi-yong Zhan Gao-miao wang han-zhong HUA Wei Key Laboratory for Biology and Genetic Improvement of Oil Crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academyof Agriculture SciencesWuhan 430062P.R.China

In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

关键词： chloroplast DNA mitochondrial DNA organelle isolation rapeseed

Cloning and Sequence Analysis of Prophenoloxidase from Haemocytes of the Red Swamp Crayfish,Procambarus clarkii

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2009年第3期8卷 369-379页

作者： LI Yan-he ZHENG Fang-liang CHEN Hong-quan wang han-zhong wang Liu-quan XUDi-ping Anhui Agricultural University Hefei 230036 P.R.China State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 P.R.China The Fishery Institute Anhui Academy of Agricultural Sciences Hefei 230031 P.R.China Huibei Key Laboratory of Animal Embryo & Molecular Breeding Hubei Academy of Agricultural Sciences Wuhan 430064 P.R.China

The full-length cDNA sequence of prophenoloxidase was obtained through RACE technology. The complete cDNA sequence is 3 721-bp long, containing an open reading frame （ORF） of 1 881 bp, a 154-bp 5′-untranslated region, and a 1 686- bp 3′-untranslated region with three potential functional poly（A） signals （AATAAA）. The molecular mass of the deduced amino acid sequence （627 aa） was 72.3 kDa with an estimatedpI of 5.88. It contained putative copper-binding sites （copper A： 131, 135, 167 and copper B： 301,305, 341）, and a tentative complement-like motif （GCGWPDHL）. Eight potential N-linked glycosylation sites were predicted to be present in P. clarkii prophenoloxidase. Similar to those in other arthropod prophenoloxidases reported so far, no signal peptide was detected in the crayfish prophenoloxidase. The phylogenetic trees confirmed that P. clarkii prophenoloxidase was most closely related to that of freshwater crayfish P. leniusculus and more closely related to other crustacean prophenoloxidases from shrimp, prawn, and lobster than to the insect prophenoloxidases. Besides, two putative introns were found in this sequence of genomic DNA.

关键词： prophenoloxidase cDNA Procambarus clarkii nucleotide sequence copper-binding sites intron

Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第4期9卷 488-496页

作者： CHEN Bo wang Tai-xia wang han-zhong LI Yun-chang YAN Xiao-hong wang Li-jun WEIWen-hui Institute of Oil Crops Chinese Academy of Agricultural Sciences/Key Laboratory for Biological Sciences of Oil Crops Ministry of Agriculture Wuhan 430062 P.R.China College of Life Sciences Henan Normal University Xinxiang 453007 P.R.China

AINTEGUMENTA （ANT） gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B. napus cultivar zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B. napus, namely ***.a （GenBank accession no. DQ211969） and ***.b （accession no. DQ211970）. They shared 87.1 and 83.4% amino acid identity with ANT of A. thaliana, respectively. There is 87.4% amino acid identity between ***.a and ***.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B. napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B. napus.

关键词： AINTEGUMENTA gene Brassica napus BnaANT gene yield-related gene quantitative real-time PCR

Combining Ability and Genetic Effects of Germination Traits of Brassica napus *** Waterlogging Stress Condition

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第7期9卷 951-957页

作者： CHENG Yong GU Min CONG Ye ZOU Chong-shun ZhanG Xue-kun wang han-zhong Oil Crops Research Institute Chinese Academy of Agriculture Sciences/Key Laboratory for Oil Crop Biology Ministry of Agriculture Wuhan 430062. P.R.China Agricultural College Yangtze University Jingzhou 434023 P.R. China

Cross combinations from six rapeseed cultivars and lines were evaluated under waterlogging stress condition in order to understand the genetic mechanism of waterlogging tolerance of Brassica napus *** provide reasonable improvement *** were six germination traits investigated on combining ability and heritability using complete diallel crossing method designed for 30 combinations from those six cultivars and *** traits included relative root length,stem length,fresh weight per plant,survival rate,electrical conductivity,and vigor *** flooding treatment,the six traits of parents and F1 were *** general combining ability（GCA） and special combining ability（SCA） of germination traits were analyzed using Griffing I *** 30 cross combinations,the GCA was significantly different among six waterlogging resistance *** SCA of these traits was significantly different except the SCA of electrical *** a representative trait of waterlogging tolerance in rapeseed,relative vigor index had the highest narrow heritability and relatively low broad *** cultivars zhongshuang 9 and P79 had higher tolerance potential to waterlogging *** can be concluded that combining ability and genetic effects of relative vigor index during germination stage could be used to identify the waterlogging tolerance of rapeseed in breeding program.

关键词： Brassica napus L. relative vigor index waterlogging tolerance germination trait combining ability diallel crossing

Effect of Gingko Injection on the Expression of NF-κB in Pulmonary Vessels of Rats with Portal Hypertension

在线全文

学校读者我要写书评

暂无评论

生物医学工程学杂志 2005年第S1期22卷 107-108页

作者： Chao wang Juan han zhong Yan LIU Zhen YANG (Department of Integrative Surgery, Tongji Hospital, Tong ji Medical College, Hua zhong University of Science and Technology, Wuhan 430030, China) Department of Integrative Surgery Tongji Hospital Tong ji Medical College Hua Zhong University of Science and Technology Wuhan 430030 China

1 IntroductionDomestic scholars have originated the conception of'portal hypertensive vasculopathy ( PHV)', which concerns multiple organ vasculopathies in portal hypertensive patients. Portal hypertensive pulmonary vasculopathy is also a kind of internal organ vasculopathy. The transcription factor nuclear factor κB (NF-κB) regulates the expressions of genes that contribute to acute inflammatory reactions in endothelium. Few have reported about the expression of NF-κB in pulmonary vessels of rats related with portal hypertension (PH). Firstly we detected the expression of NF-κB in pulmonary vessels of rats with PH induced by biliary cirrhosis. Then through injecting of gingko (GI) into abdominal cavity, we found that NF-κB's expression was inhibited. We propose that NF-κB may play an important role in the pathogenesis of PHV and GI can hinder the improvement of PHV through inhibiting the expression of NF-κB. This outcome may provide certain theoretic basis for a novel therapy of portal hypertension.

关键词： Effect of Gingko Injection on the Expression of NF GI B in Pulmonary Vessels of Rats with Portal Hypertension

Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei

在线全文

学校读者我要写书评

暂无评论

Effect of probiotics on growth performance and digestive enz...

第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Yan-Bo wang Jian-zhong han

The effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei was *** bacteria and Bacillus *** added to shrimp basal diets as probiotics at three concentrations:T-1,2 g Kg(1 g Kgiyophilized photosynthetic bacteria cells (PSB)and 1 g Kglyophilized Bacillus sp.(BS));T-2,10 g Kg(5 g KgPSB and 5 g KgBS);and T-3, 20 g Kg(10 g KgPSB and 10 g KgBS).Twelve aquaria with three replicates for each treatment group and the Control group were *** 28 days,shrimp receiving the diets supplemented with probiotics showed’ significantly better growth performance than those fed the basal diet(Control).The mean digestive enzyme activity of each treatment groups was significantly different(P<0.05)from that of the Control. The protease activity of T-2 and T-3 was significantly higher compared with T-1 and the ***, there was no significant difference between T-2 and *** amylase activity of T-2 was highest and significantly different(P<0.05)from that of the Control and *** treatment groups had significantly higher lipase and cellulase activity compared to the Control.

关键词： Probiotics Penaeus vannamei Growth performance Digestive enzyme

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

装备备件两级闭环供应链库存优化与分析

系统工程理论与实践 2010年第12期30卷 2309-2314页

作者：聂涛盛文王晗中空军雷达学院研究生管理大队武汉430019 空军雷达学院陆基预警监视装备系武汉430019

研究备件两级闭环供应链系统结构与备件运转流程.通过建立单项备件供应可用度模型,构建了两级闭环供应链库存系统优化模型,并运用边际效能分析算法合理配置基层级和基地级备件库存量,实现了在有限购置费用资源约束条件下的系统供应效能... 详细信息

研究备件两级闭环供应链系统结构与备件运转流程.通过建立单项备件供应可用度模型,构建了两级闭环供应链库存系统优化模型,并运用边际效能分析算法合理配置基层级和基地级备件库存量,实现了在有限购置费用资源约束条件下的系统供应效能最大化,分析得出提高两级维修能力可以改善系统供应效能.

关键词：装备备件闭环供应链库存优化供应可用度 METRIC模型

在线全文

学校读者我要写书评

暂无评论

中国油料产业发展的现状、问题与对策

中国油料作物学报 2005年第4期27卷 100-105页

作者：王汉中中国农业科学院油料作物研究所湖北武汉430062

从我国油料生产、消费、进出口情况及进入WTO后所面临的国际国内形势等方面,分析了我国油料产业发展的现状和存在的问题,提出了“十一五”我国油料产业发展的对策。

关键词：油料产业现状与问题对策

Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第7期9卷 942-950页

作者： Zhan Gao-miao,TONG Jin,wang han-zhong and HUA Wei Key Laboratory for Biological Sciences of Oil Crops,Ministry of Agriculture/Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,P.R.China [a] Key Laboratory for Biological Sciences of Oil Crops Ministry of Agriculture/Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R. China

In eukaryotes,14-3-3 proteins constitute a family of ubiquitous regulatory *** play very important roles in many cell ***,their enconding genes and roles in plants remain to be *** this paper,four 14-3-3 genes from Brassica napus *** obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 *** phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2 belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon *** transcript levels of four Bn1433 genes were analyzed in different organs,various stress conditions and some hormone treatments by real-time *** result showed that all the Bn1433s were expressed constitutively in roots,stems,leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF（day after flowering）.By the analysis of real-time PCR,different expression patterns of Bn1433s were studied in various stress conditions and hormone *** was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.

关键词： 14-3-3 expression pattem abiotic stress Brassica napus L.