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Early Detection of the Emerging SARS-CoV-2 BA.2.86 Lineage Through Wastewater Surveillance Using a Mediator Probe PCR Assay—Shenzhen City,Guangdong Province,China,2023

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China CDC weekly 2024年第15期6卷 332-338,I0008,I0009页

作者： Chen Du Yuejing Peng Ziquan Lyu Zhijiao Yue Yulin Fu Xiangjie Yao Jinzhen Tang Guixian Luo Chenxi Gao Shisong Fang Xiaolu Shi chengsong wan Yinghui Li Qinghua Hu Shenzhen Center for Disease Control and Prevention Shenzhen CityGuangdong ProvinceChina BSL-3 Laboratory(Guangdong) Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou CityGuangdong ProvinceChina Department of Public Health Laboratory Sciences School of Public HealthHengyang Medical SchoolUniversity of South ChinaHengyang CityHunan ProvinceChina School of Public Health Shanxi Medical UniversityTaiyuan CityShanxi ProvinceChina

Introduction:The emergence of the new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron sublineage,BA.2.86,has sparked global public health concerns for its potential heightened transmissibility and immune *** data from Shenzhen’s city-wide wastewater surveillance system,we highlight the presence of the BA.2.86 lineage in ***:A mediator probe polymerase chain reaction(PCR)assay was developed to detect the BA.2.86 lineage in wastewater by targeting a specific mutation(Spike:A264D).Between September 19 and December 10,2023,781 wastewater samples from 38 wastewater treatment plants(WWTPs)and 9 pump stations in ten districts of Shenzhen were *** multiple short-amplicon sequencing,three positive samples were ***:The BA.2.86 lineage was identified in the wastewater of Futian and Nanshan districts in Shenzhen on December 2,*** December 2 to 10,a total of 21 BA.2.86-positive wastewater samples were found across 6 districts(Futian,Nanshan,Longhua,Baoan,Longgang,and Luohu)in *** weighted average viral load of the BA.2.86 lineage in Shenzhen’s wastewater was 43.5 copies/L on December 2,increased to 219.8 copies/L on December 4,and then decreased to approximately 100 copies/L on December 6,8,and ***:The mediator probe PCR assay,designed for swift detection of low viral concentrations of the BA.2.86 lineage in wastewater samples,shows promise for detecting different SARS-CoV-2 *** surveillance could serve as an early detection system for promptly identifying specific SARS-CoV-2 variants as they emerge.

关键词： wastewater Wastewater district

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Genomic sequencing and analysis of the first imported Middle East Respiratory Syndrome Coronavirus(MERS CoV) in China

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Science China(Life Sciences) 2015年第8期58卷 818-820页

作者： XIE Qian CAO YuJuan SU Juan WU XianBo wan chengsong KE ChangWen ZHAO Wei ZHANG Bao The lab of Biosafety Level 3 School of Public Health and Tropical Medicine Southern Medical University Research Center for Pathogens Detection Technology of Emerging Infectious Diseases Guangdong Provincial Center for Disease Control and Prevention

Dear Editors,Middle East Respiratory Syndrome （MERS） has spread to more than 23 countries since the first case of a MERS coronavirus （MERS CoV） infection was confirmed in Sep 2012 [1]. Because MERS CoV has led to mortality as high as 40%, it has become one of the most important emerging pathogens threatening human health. Recently, the outbreak of MERS CoV, which resulted in 172 infections and 27 deaths in South Korea, was the largest outside Saudi Arabia. A 44-year-old man, had been exposed to the first confirmed MERS case who shared a ward with the man＇s father in this outbreak, travelled from South Korea to China, becoming the first imported MERS case reported in China. Here, we report the sequencing and analysis of the MERS CoV ge- nome from this case. Viral genome sequencing provides information vital to tracing the pathogenic source, predict- ing viral virulence, and following the pattern of viral evolution, variation, and transmission [2-4].

关键词：基因组测序呼吸综合征冠状病毒中国中东进口

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Piperlongumine Inhibits Zika Virus Replication In vitro and Promotes Up-Regulation of HO-1 Expression, Suggesting An Implication of Oxidative Stress

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Virologica Sinica 2021年第3期36卷 510-520页

作者： Weizhi Lu Linjuan Shi Jing Gao Huimin Zhu Ying Hua Jintai Cai Xianbo Wu chengsong wan Wei Zhao Bao Zhang Biosafety Level-3 Lab School of Public HealthSouthern Medical UniversityGuangzhou 510515China Department of Epidemiology School of Public HealthSouthern Medical UniversityGuangzhou 510515China

Owing to the widespread distribution of mosquitoes capable of transmitting Zika virus, lack of clinical vaccines and treatments, and poor immunity of populations to new infectious diseases, Zika virus has become a global public health concern. Recent studies have found that Zika virus can continuously infect human brain microvascular endothelial *** cells are the primary components of the blood–brain barrier of the cerebral cortex, and further infection of brain tissue may cause severe damage such as encephalitis and fetal pituitary disease. The present study found that a biologically active base, piperlongumine(PL), inhibited Zika virus replication in human brain microvascular endothelial cells, Vero cells, and human umbilical vein endothelial cells. PL also significantly increased heme oxygenase-1(HO-1) gene expression, while silencing HO-1 expression and using the reactive oxygen species scavenger, N-acetylcysteine, attenuated the inhibitory effect of PL on Zika virus replication. These results suggest that PL induces oxidative stress in cells by increasing reactive oxygen species. This, in turn, induces an increase in HO-1 expression, thereby inhibiting Zika virus replication. These findings provide novel clues for drug research on the prevention and treatment of Zika virus.

关键词： Piperlongumine Heme oxygenase-1(HO-1) Reactive oxygen species Zika virus(ZIKV) Human brain microvascular endothelial cells

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Desmoglein 2(DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia

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Virologica Sinica 2021年第6期36卷 1400-1410页

作者： Jing Zhang Kui Ma Xiangyu wang Yinbo Jiang Shan Zhao Junxian Ou Wendong Lan Wenyi Guan Xiaowei Wu Heping Zheng Bin Yang chengsong wan Wei Zhao Jianguo Wu Qiwei Zhang Guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou510632China Guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou510515China Guangdong Provincial Dermatology Hospital Southern Medical UniversityGuangzhou510091China

Human adenovirus type 55(HAdV-B55) is a re-emergent acute respiratory disease pathogen that causes adult communityacquired pneumonia(CAP). Previous studies have shown that the receptor of HAdV-B14, which genome is highly similar with HAdV-B55, is human Desmoglein 2(DSG2). However, whether the receptor of HAdV-B55 is DSG2 is undetermined because there are three amino acid mutations in the fiber gene between HAdV-B14 and HAdV-B55. Here, firstly we found the 3T3 cells, a mouse embryo fibroblast rodent cell line which does not express human DSG2, were able to be infected by HAdV-B55 after transfected with pcDNA3.1-DSG2, while normal 3T3 cells were still unsusceptible to HAdV-B55 infection. Next, A549 cells with h DSG2 knock-down by siRNA were hard to be infected by HAdV-B3/-B14/-B55, while the control siRNA group was still able to be infected by all these types of HAdVs. Finally, immunofluorescence confocal microscopy indicated visually that Cy3-conjugated HAdV-B55 viruses entered A549 cells by binding to DSG2 ***, DSG2 is a major receptor of HAdV-B55 causing adult CAP. Our finding is important for better understanding of interactions between adenoviruses and host cells and may shed light on the development of new drugs that can interfere with these processes as well as for the development of potent prophylactic vaccines.

关键词： Human adenovirus type 55(HAdV-B55) Severe community-acquired pneumonia Adenovirus receptor Desmoglein 2(DSG2)

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Identification and Typing of Respiratory Adenoviruses in Guangzhou, Southern China Using a Rapid and Simple Method

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Virologica Sinica 2013年第2期28卷 103-108页

作者： Guiyuan Han Hongling Niu Suhui Zhao Bing Zhu Changbing wang Yungang Liu Mingjie Zhang Shu Yang Feitong Liu chengsong wan Qiwei Zhang School of Public Health and Tropical Medicine Southern Medical University Central Laboratory Guangzhou Women and Children's Medical Center

Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China.

关键词： Molecular epidemiology Identification Typing Southern China

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深圳市基于污水流行病学的新型冠状病毒奥密克戎变异株感染者来源追踪案例分析

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中华医学杂志 2024年第4期104卷 302-307页

作者：彭悦景李迎慧杜琛郭寅生宋江腾贾春艳张鑫刘慕军王子苗刘斌严淑兰杨煜祥唐湘良林革新李学云张勇袁建辉许世宽陈传德逯建华邹旋万成松扈庆华南方医科大学公共卫生学院生物安全研究中心，广州510515 深圳市疾病预防控制中心病原微生物检测所，深圳518055 深圳市疾病预防控制中心突发公共卫生应急部，深圳518055 深圳市生态环境局水生态环境处，深圳518040 深圳市福田区水务局，深圳518035 深圳市福田区水务局供排水管理科，深圳518035 深圳市南山区水务局排水与灾害防御科，深圳518052 深圳市生态环境局罗湖管理局，深圳518001 深圳市生态环境局罗湖管理局环境管理科，深圳518001 深圳市福田区疾病预防控制中心，深圳518040 深圳市福田区疾病预防控制中心微生物检验科，深圳518040 深圳市南山区疾病预防控制中心，深圳518054 深圳市南山区疾病预防控制中心传染病防制科，深圳518054 深圳市罗湖区疾病预防控制中心，深圳518020 深圳市疾病预防控制中心，深圳518055 南方医科大学公共卫生学院生物安全研究中心，广州510515 南方医科大学公共卫生学院广东省热带病研究重点实验室BSL-3实验室，广州510515

污水流行病学(WBE)是一门新兴学科,已被应用于药物滥用追踪和传染病病原体监测。在新型冠状病毒(简称新冠病毒)感染疫情期间,WBE被应用于监测疫情流行趋势和提供早期预警。为了及时发现隐匿的新冠病毒感染者,防止其在社区的传播,深圳市... 详细信息

污水流行病学(WBE)是一门新兴学科,已被应用于药物滥用追踪和传染病病原体监测。在新型冠状病毒(简称新冠病毒)感染疫情期间,WBE被应用于监测疫情流行趋势和提供早期预警。为了及时发现隐匿的新冠病毒感染者,防止其在社区的传播,深圳市于2022年7月26日至11月30日在福田、南山、罗湖和盐田区的口岸地带、城中村、居民小区等重点场所布设369个污水采样点位,覆盖193万人口,开展污水监测新冠病毒的工作。每日在用水高峰时段进行3 h连续采样,采用聚乙二醇沉淀法和RT-qPCR进行污水病毒富集浓缩及新冠病毒核酸检测,并形成了阳性水样处置流程。本文旨在介绍深圳市基于城市污水开展新冠病毒感染者来源追踪的相关案例,在罗湖区洪湖水质净化厂污水监测有效发挥了早期预警作用并实现感染者来源追踪;在福田区福田南路阳性水样处置中获得监测点位布设的重要经验;在南山区南山村污水监测中揭示了隐匿感染者的存在。分享基于WBE的新冠病毒监测和感染者追踪,避免新冠病毒感染者在社区传播的经验,总结污水监测的优势和应用前景,为未来具有肠道排毒的新发或再发传染病病原体监测提供新的研究思路。

关键词：污水流行病学污水监测新型冠状病毒

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“新医改”实施前后佛山市某社区卫生服务中心的基本药物使用情况分析

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中国药房 2017年第18期28卷 2512-2516页

作者：王珍姝万成松南方医科大学公共卫生学院广州510515 佛山市顺德区伦教社区卫生服务中心广东佛山528308

目的:为"新医改"的进一步推进和国家基本药物制度的进一步实施和完善提供参考。方法:采取系统抽样法,随机抽取佛山市某社区卫生服务中心"新医改"前后(2012-2015年)门诊处方1 728张,对处方用药数量、处方合理用药指标情况、处方费用、主... 详细信息

目的:为"新医改"的进一步推进和国家基本药物制度的进一步实施和完善提供参考。方法:采取系统抽样法,随机抽取佛山市某社区卫生服务中心"新医改"前后(2012-2015年)门诊处方1 728张,对处方用药数量、处方合理用药指标情况、处方费用、主要疾病构成及其治疗费用等进行统计和对比分析,研究其变化规律及特点。结果:单张处方药品平均品种数和基本药物平均品种数均逐年下降;与2012年相比,抗菌药物处方比例、二联及以上使用抗菌药物处方比例、注射剂处方比例及输液处方比例均显著降低,患者就医费用显著下降,药品费用、医保处方报销金额则有不同程度上升,差异均有统计学意义(P<0.05);多元线性回归分析(逐步法)显示,患者就医费用与处方合理性等密切相关;主要疾病构成方面,呼吸道疾病、心脑血管系统疾病、消化道疾病相关处方占比居前3位,而相关处方占比呼吸道疾病、消化道疾病逐年下降,心脑血管系统疾病则逐年上升。结论:"新医改"的推进和国家基本药物制度的实施对社区卫生服务中心的合理用药水平和医疗质量提升起到正向促进作用,但社区卫生服务中心单张处方药物数量偏多、疾病诊治能力不足、药品遴选及管理缺陷等问题仍然存在。

关键词：新医改国家基本药物制度社区卫生服务中心合理用药

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副溶血性弧菌tdh基因的分子信标PCR技术检测

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中国公共卫生 2006年第12期22卷 1475-1477页

作者：万成松谭翰清温文川南方医科大学公共卫生与热带医学学院微生物学系广州510515 广州出入境检验检疫局食品检验中心

目的采用分子信标PCR技术进行副溶血性弧菌tdh基因检测。方法在反应体系中加入分子信标探针。对13株副溶血性弧菌和其他细菌分别进行tdh基因实时和终点法荧光检测。结果2株副溶血性弧菌和阳性质粒的终点法检测荧光值分别为114．9，95．2... 详细信息

目的采用分子信标PCR技术进行副溶血性弧菌tdh基因检测。方法在反应体系中加入分子信标探针。对13株副溶血性弧菌和其他细菌分别进行tdh基因实时和终点法荧光检测。结果2株副溶血性弧菌和阳性质粒的终点法检测荧光值分别为114．9，95．2，90．0。实时PCR检测的CT值分别为26．2，26．8，32．0。其他肠道细菌终点法检测荧光值为47．0～69．1；CT值〉32．0或无值，与琼脂糖电泳分析结果一致。结论分子信标PCR技术可以准确、快速、实时、简便地进行副溶血性弧菌tdh基因检测。

关键词：副溶血性弧菌(VP) 分子信标 tdh基因实时PCR

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广东地区TTV感染情况及基因分型

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中华传染病杂志 2000年第3期18卷 183-185页

作者：王虹王省良万成松彭华国第一军医大学基础部生物医学诊断研究中心广州510515 第一军医大学微生物学教研室广州510515

目的　调查广东地区TT病毒 (TTV)感染流行情况和基因分型。方法　选取TTVORF1的保守序列作内外引物 ,Ⅰ型、Ⅱ型同源性极高的序列nt2 46 4～nt2 5 2 0作包被探针 ,异源性大于 5 0 %的序列nt2 12 5～nt2 15 9作显色探针Ⅰ和显色探针Ⅱ ,... 详细信息

目的　调查广东地区TT病毒 (TTV)感染流行情况和基因分型。方法　选取TTVORF1的保守序列作内外引物 ,Ⅰ型、Ⅱ型同源性极高的序列nt2 46 4～nt2 5 2 0作包被探针 ,异源性大于 5 0 %的序列nt2 12 5～nt2 15 9作显色探针Ⅰ和显色探针Ⅱ ,采用微板核酸杂交 ELISA技术对 2 80例肝功能损害的肝炎患者血清进行TTV检测及TTV基因分型研究。结果　检测出 44例TTV阳性患者 ,检出率为 15 .71%。对 44例TTV阳性患者的标本进行分型 ,Ⅰ型、Ⅱ型和混合型比例为 30∶4∶10。慢性肝炎中TTV感染者与急性肝炎中TTV感染者比例为 2 8∶16。结论　在广东地区的肝炎疾病中 ,TTV有较高的发病率 ,且慢性肝炎感染TTV的机会多于急性肝炎 ,TTV可分为两个型 :Ⅰ型和Ⅱ型 ,Ⅰ型占 6 8.18% ,Ⅱ型占 9.0 9% ,Ⅰ型明显多于Ⅱ型。微板核酸杂交 ELISA技术特异性强 ,灵敏度高 ,分型准确快速。

关键词：微板核酸杂交 TTV感染基因分型

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肠出血型大肠埃希菌O157:H7 ppk基因缺失株的构建及其生物学特性分析

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南方医科大学学报 2014年第6期34卷 904-908页

作者：韩朋孙琦赵素慧张其威万成松南方医科大学公共卫生与热带医学学院BSL-3实验室广东广州510515

目的利用Red重组系统构建肠出血型大肠埃希菌(EHEC)O157:H7 ppk基因缺失株,并研究其生物特性。方法设计并合成一对同源臂引物,每条引物5'端与ppk基因同源,3'端与卡那霉素抗性基因同源,扩增卡那霉素抗性基因片段;制备含有pKD46质粒的EHEC... 详细信息

目的利用Red重组系统构建肠出血型大肠埃希菌(EHEC)O157:H7 ppk基因缺失株,并研究其生物特性。方法设计并合成一对同源臂引物,每条引物5'端与ppk基因同源,3'端与卡那霉素抗性基因同源,扩增卡那霉素抗性基因片段;制备含有pKD46质粒的EHEC O157:H7 EDL933w感受态细菌,然后将该抗性基因片段电击转化入制备的感受态菌株中;利用pKD46介导的Red重组系统,通过同源重组替换EHEC O157:H7 EDL933w ppk基因,PCR和测序验证;通过革兰染色、测D600值、吉姆萨染色比较EHEC O157:H7 EDL933w野生株和突变株的形态结构、生长能力和黏附性。结果构建了ppk基因缺失并含有卡那霉素抗性的EHEC O157:H7 EDL933w突变菌株(EHEC O157:H7 EDL933wΔppk),初步探索了EHEC O157:H7 EDL933w野生株和突变株的生物学特性。结论本研究运用Red重组系统构建了肠出血型大肠埃希菌O157:H7 ppk基因缺失株,为进一步研究肠出血型大肠埃希菌中ppk基因的调控机制奠定了基础。

关键词： EHEC O157 H7 ppk基因 Red重组系统基因突变

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