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组织工程化人工神经实验研究

中国创伤骨科杂志 2000年第4期2卷 290-292页

作者：沈尊理 BERGER Alfred HIERNER Robert LOHMEYER Joern ALLMELING Christina walter f.gerhard 上海市第一人民医院整形外科上海市200080 德国汉诺威医学院整形手重建外科德国汉诺威医学院神经病理研究所

目的研究组织工程化人工神经修复大鼠2.5cm长坐骨神经缺损的效果。方法 21只2月龄Lewis lw雌性大鼠随机分成三个神经移植组,每组7只。A组:种植同源雪旺细胞并具有内部支架结构的胶原神经管,即组织工程化人工神经。B组:无雪旺细胞但具... 详细信息

目的研究组织工程化人工神经修复大鼠2.5cm长坐骨神经缺损的效果。方法 21只2月龄Lewis lw雌性大鼠随机分成三个神经移植组,每组7只。A组:种植同源雪旺细胞并具有内部支架结构的胶原神经管,即组织工程化人工神经。B组:无雪旺细胞但具有内部支架结构的胶原神经管。C组:自体神经移植组。术后六个月,进行系列神经电生理监测,神经肌肉组织学观察,S-100和神经微丝蛋白(Neurofilament)免疫组化染色,轴突计数等检查。结果在A组和C组移植神经上均能诱发出波幅明显的神经肌肉复合动作电位(CMAP),再生轴突已通过移植神经全长,远端肌肉轻度萎缩。而B组中没有或仅记录到极小波幅的CMAP,移植神经远端结缔纤维组织增生,再生轴突罕见,所支配肌肉明显萎缩。结论初步结果显示:组织工程化人工神经可用来修复大鼠长段神经缺损。

关键词：组织工程神经移植雪旺氏细胞培养神经管

Dehydrated Natural feed for Cats: Influence on Apparent Digestibility Coefficient, feces Scores, and Serum Immune, Antioxidant, and Metabolic Biomarkers

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food and Nutrition Sciences 2024年第7期15卷 594-611页

作者： Bruno Giorgio de Oliveira Cécere Guilherme Luiz Deolindo Bianca f. Bissacotti Priscila Marquezin Copetti Maria Rosa Chitolina Schetinger Bruna Klein Matheus Delamea Baldissera Thiago Pereira Ribeiro walter Hugo Cuelho Suarez Daniel Pigatto Monteiro Aleksandro Schafer Da Silva Graduate Multicenter Program in Biochemistry and Molecular Biology University of the State of Santa Catarina Lages Brazil Department of Biochemistry and Molecular Biology Universidade Federal de Santa Maria (UFSM) Santa Maria Brazil Department of Animal Science Universidade Federal de Santa Maria (UFSM) Chapec Brazil Department of Health Science Universidade Franciscana (UFN) Santa Maria Brazil Department of Animal Nutrition Tectron-Tecnologia e Inovao Toledo Brazil

The present study aimed to determine whether natural dehydrated feed (DNf) can replace dry extruded feed while preserving the required daily intake, nutrient digestibility and benefits for immunity and antioxidant response in cats. The animals were divided into two groups of seven animals each. One group received DNf, and the other consumed commercial extruded dry feed (DCf). The experiment lasted 45 days and we measured daily consumption and collected blood and feces samples. The animals in the DNf group showed greater digestibility of fiber and mineral matter than DCf. Cats in the DNf group had a higher volume of feces. DNf cats had lower leukocyte and granulocyte counts and cholesterol levels. Serum enzymatic antioxidant responses and non-enzymatic were higher in DNf cats. Cats in the DNf group showed lower serum acute phase proteins in addition to a tendency toward a higher concentration of transferrin than in the DCf group. fecal Escherichia coli and total coliform counts of cats fed DNf were higher than DCf. We conclude that the daily consumption of cats is adequate. This feed had greater digestibility of fiber and mineral matter, in addition to an improvement in antioxidant and immunological responses.

关键词： Alternative feed Antioxidant Companion Animals Health

Trace-element geochemistry and S–O isotopes in the fluorite-barite mineralization of Merguechoum,Moroccan eastern Meseta:insights into ore genesis to the Pangea rifting

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Acta Geochimica 2023年第3期42卷 435-452页

作者： Mohammed Cherai Larbi Rddad fouad Talbi Benjamin f.walter faculte´des Sciences Laboratoire de GeosciencesGeo-Environnement et Prospection Miniere et HydriqueBP 71760000 OujdaMaroc Earth and Planetary Science Division Physical Science DepartmentKingsborough Community College of the City University of New York(CUNY)2001 Oriental BoulevardBrooklynNY 11235–2398USA Chair of Economic Geology and Geochemistry Karlsruhe Institute of Technology(KIT)Adenauerring 20B76131 KarlsruheGermany Laboratory for Environmental and Raw Material Analyses(LERA) Adenauerring 20B76131 KarlsruheGermany

The Merguechoum fluorite-barite mineralization,located in the Eastern Meseta of Morocco,is hosted in the Late Hercynian *** ore consists of fine crystals of fluorite 1,massive barite 1,euhedral crystals of fluorite 2,and barite 2 with calcite and minor quartz and *** Merguechoum ore deposits have never been *** study was the first contribution that studied the genesis of fluorite and *** ore occurs as dissemination within granite intrusion and also fills the NE-SWtrending meter-sized fractures and *** values of the total Rare Earth Elements and Yttrium(REY)and the ratios of LREY/HREY,Y/Ho,Tb/Ca,and Tb/La indicate that the Merguechoum fluorite precipitated from hydrothermal fluids,likely basinal brines,which interacted with the Hercynian *** REY data indicate that the ore-forming fluids of the early stage have intensely interacted with the Hercynian granite compared to those of the late ore *** gradual decrease in the europium(Eu/Eu^(*)),yttrium(Y/Y^(*)),and cerium(Ce/Ce^(*))anomalies and a low concentration ofΣREY observed in the second ore stage compared to the first ore stage suggest an increase in p H and fO_(2)and by inference a decrease in temperature during the evolution of the hydrothermal *** evolution could be explained by fluid mixing between the ascending basinal hydrothermal fluids and the diluted sulfate-rich meteoric water barite separates from selected samples reveal that the dissolved sulfates(SO_(4)^(2-))were derived from Permian–Triassic sulfates and/or coeval poreseawater *** proposed fluid mixing triggered the precipitation of an early-stage f-Ba assemblage followed by the second-stage f-Ba *** fieldwork,REY inventories,and isotope data point to the ore genesis during the Permian–Triassic extensional tectonic activity concerning the Pangea *** extensional tectonic environment is likely the driving force that mobilized a large amount of the ore

关键词： fluorite-barite veins Geochemistry of REY and trace elements O–S isotopes Late hercynian granite Pangea rifting Merguechoum Eastern Meseta of Moroccan

Treatment of colonic varices and gastrointestinal bleeding by recanalization and stenting of splenic-vein-thrombosis:A case report and literature review

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World Journal of Gastroenterology 2023年第24期29卷 3922-3931页

作者： Lisa-Michaela füssel Rene Müller-Wille Patrick Dinkhauser walter Schauer Harald Hofer Department of Internal Medicine Ⅰ Gastroenterology and HepatologyKlinikum Wels-GrieskirchenWels 4600Austria Department of Radiology Klinikum Wels-GrieskirchenWels 4600Austria Department of Abdominal Surgery Klinikum Wels-GrieskirchenWels 4600Austria

BACKGROUND Splenic vein thrombosis is a known complication of *** can lead to increased blood flow through mesenteric *** segmental hypertension may result in the development of colonic varices(CV)with a high risk of severe gastrointestinal *** clear guidelines for treatment are lacking,splenectomy or splenic artery embolization are often used to treat *** vein stenting has been shown to be a safe *** SUMMARY A 45-year-old female patient was admitted due to recurrent gastrointestinal *** was anemic with a hemoglobin of 8.0 g/*** a source of bleeding,CV were *** tomography scans revealed thrombotic occlusion of the splenic vein,presumably as a result of a severe acute pancreatitis 8 years *** a selective angiography,a dilated mesenterial collateral leading from the spleen to enlarged vessels in the right colonic flexure and draining into the superior mesenteric vein could be *** hepatic venous pressure gradient was within normal *** an interdisciplinary board,transhepatic recanalization of the splenic vein via balloon dilatation and consecutive stenting,as well as coiling of the aberrant veins was discussed and successfully *** evaluation revealed complete regression of CV and splenomegaly as well as normalization of the red blood cell count during *** Recanalization and stenting of splenic vein thrombosis might be considered in patients with gastrointestinal bleeding due to ***,a multidisciplinary approach with a thorough workup and discussion of individualized therapeutic strategies is crucial in these difficult to treat patients.

关键词： Pancreatitis-induced splenic vein thrombosis Gastrointestinal hemorrhage Colonic varices Splenic vein stenting Segmental/sinistral hypertension Case report

On COVID-19 and Membrane Lipids and Public Health

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粮油食品科技 2022年第3期30卷 55-58页

作者： Yi-qun WANG Mark JOHNSON walter f.SCHMIDT Hong-wei REN Michael A CRAWfORD Department of Metabolism Digestion and ReproductionChelsea and Westminster Hospital CampusImperial CollegeLondon SW109NHUK United States Department of Agriculture Agricultural Research Service BeltsvilleMD 20705USA Division of Virology Department of PathologyUniversity of CambridgeCambridge CB21QPUK

Coronavirus has a lipid *** replication requires hijacking the RNA tools of the host to synthesize virion protein,that then has to be wrapped in a lipid membrane to enable the budding off which extends the *** studies implicate certain essential fatty acids with replication suppression *** lipid membrane is commonly thought of as a fatty barrier to water *** is however highly ordered and compositionally specific to cellular and sub cellular *** will likely also be an optimum specificity for the viral *** DNA,RNA and protein compositions are not affected by diet,the lipid membrane ***,the greater sensitivity of males over females to inadequacy of these essential fatty acids and membrane integrity has been known since the 1960 *** evidence that arachidonic and docosahexaenoic acids exhibiting anti-viral,immune,anti-inflammatory,blood pressure control and resolvin activity,their status needs to be urgently examined in relation to the prevention and therapy for *** would also be advisable to re-assess food *** lipid requirements for the membrane rich systems as in the brain,nervous,vascular and immune systems have not been *** is little doubt these were significant in shaping the human genome over several million *** from such conditions would be predicted to put populations at risk to disorder and infection,with males being more at risk than females.

关键词： coronavirus Covid-19 arachidonic docosahexaenoic membrane lipid male-female risk public health

康复卫生政策和系统研究对加强卫生系统中康复服务的重要性:呼吁采取行动并加速开展

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中国康复 2024年第4期39卷 251-253页

作者： walter R.frontera Wouter DeGroote Abdul Ghaffar 康复卫生政策和系统研究小组 WHO康复培训与研究合作中心(武汉) 不详

具体机构及作者名单如下:美国物理医学与康复杂志(WRf);世界卫生组织康复计划(WDG);世界卫生组织卫生政策和系统研究联盟(WAG);康复卫生政策和系统研究小组(合作期刊主编按字母顺序排列):Iben Axen,DC,博士(脊椎按摩疗法和手法治疗)、Mu... 详细信息

具体机构及作者名单如下:美国物理医学与康复杂志(WRf);世界卫生组织康复计划(WDG);世界卫生组织卫生政策和系统研究联盟(WAG);康复卫生政策和系统研究小组(合作期刊主编按字母顺序排列):Iben Axen,DC,博士(脊椎按摩疗法和手法治疗)、Muhammad Ehab Azim,DPT,MS-NMPT(基础大学康复科学杂志)、Linamara Battistella,医学博士、哲学博士(Acta fisiatrica)、克里斯蒂安·博格,医学博士、哲学博士(康复医学杂志)、Ines Campos,医学博士、理学硕士(葡萄牙物理与康复医学杂志)、罗德里戈·卡斯特罗,医学博士(Revista Columbiana de Medicina físicay Rehabilitación),Joaquim Chaler,MD,Ph.D(Rehabilitación),Leighton Chan,MD,MPH(物理医学与康复档案室)。

关键词：卫生政策物理医学卫生系统康复计划哲学博士按摩疗法卡斯特罗作者名单

Pneumothorax in a Thiel cadaver model of cardiopulmonary resuscitation

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World Journal of Emergency Medicine 2023年第2期14卷 143-147页

作者： Daniel Auinger Simon Orlob Johannes Wittig Gabriel Honnef Stefan Heschl Georg feigl gerhard Prause Division of Anaesthesiology and Intensive Care Medicine 1 Department of Anaesthesiology and Intensive Care MedicineMedical University of GrazGraz 8036Austria Division of Anaesthesiology and Intensive Care Medicine 2 Department of Anaesthesiology and Intensive Care MedicineMedical University of GrazGraz 8036Austria Medical University of Graz Graz 8036Austria Research Center for Emergency Medicine Aarhus University HospitalAarhus 8200Denmark Department of Medicine Randers Regional HospitalRanders 8930Denmark Chair of Macroscopic and Clinical Anatomy Gottfried Schatz Research CenterMedical University of GrazGraz 8036Austria Institute of Morphology and Clinical Anatomy Faculty of Health/School of MedicineWitten/Herdecke UniversityWitten 58448Germany

INTRODUCTION There has been some previous research in the field of cardiopulmonary resuscitation (CPR) using cadaver models.[1]The Thiel method was developed by Professor walter Thiel (Graz, Austria) and described in 1992 and 2002. It consists of both an intravascular injection of the embalming solution and submersion of the bodies in a tank with the same solution for a determined period.[2,3]

关键词： walter cardiopulmonary bal

The impact of salps(Salpa thompsoni) on the Antarctic krill population(Euphausia superba): an individual-based modelling study

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Ecological Processes 2023年第4期12卷 38-53页

作者： Bruno walter Pietzsch Aaron Schmidt Jürgen Groeneveld Dominik Bahlburg Bettina Meyer Uta Berger faculty of Environmental Sciences Institute of Forest Growth and Forest Computer Sciences Technische Universit?t Dresden Department of Ecological Modelling Helmholtz Centre for Environmental Research-UFZ Section Polar Biological Oceanography Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Institute for Chemistry and Biology of the Marine Environment(ICBM) Carl-Von-Ossietzky University Helmholtz Institute for Marine functional Biodiversity(HIfMB)

Background Krill（Euphausia superba） and salps（Salpa thompsoni） are key macrozooplankton grazers in the Southern Ocean ecosystem. However, due to differing habitat requirements, both species previously exhibited little spatial overlap. With ongoing climate change-induced seawater temperature increase and regional sea ice loss, salps can now extend their spatial distribution into historically krill-dominated areas and increase rapidly due to asexual reproduction when environmental conditions are favorable. Understanding the potential effects on krill is crucial, since krill is a species of exceptional trophic significance in the Southern Ocean food web. Negative impacts on krill could trigger cascading effects on its predators and prey. To address this question, we combined two individual-based models on salps and krill, which describe the whole life cycle of salp individuals and the dynamic energy budget of individual krill. The resulting new model PEKRIS（PErformance of KRIll vs. Salps） simulates a krill population for 100 years under varying chlorophyll-a concentrations in the presence or absence of *** All of the investigated krill population properties（abundance, mean length, and yearly egg production） were significantly impacted by the presence of salps. On the other hand, salp density was not impacted if krill were present. The medians of krill population properties deviated during variable maximum chlorophyll-a density per year when salps were introduced by-99.9%（-234 individuals per 1000 m3） for krill density,-100%（-22,062 eggs per 1000 m3） for krill eggs and-0.9%（-0.3 mm） for mean length of *** If both species compete for the same food resource in a closed space, salps seem to inhibit krill populations. further simulation studies should investigate whether this effect prevails if different phytoplankton sizes and consumption preferences of krill are implemented. furthermore, direct predation of the two species or consumption of krill f

关键词： Euphausia superba Salpa thompsoni Individual-based model food competition Population dynamics DEB theory

Artificial nesting habitats as a conservation strategy for turtle populations experiencing global change

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Integrative Zoology 2013年第2期8卷 209-221页

作者： John P.WNEK walter f.BIEN Harold W.AVERY Department of Biology Drexel UniversityPhiladelphiaPennsylvaniaUSA

Diamondback terrapins(Malaclemys terrapin)inhabit estuaries in eastern USA and may tolerate salinity of seawater for short *** North American estuaries are adversely affected by anthropogenic impacts,such as pollution,dredging and invasion by non-native *** nesting areas have been altered or destroyed,causing terrapins to nest on roadsides and artificial islands made of dredged substrate from bottom *** by non-native plants may suppress development and reduce survival of *** Barnegat Bay,New Jersey,USA,there is a mosaic of natural and degraded terrapin nesting *** investigated the effects of dredge soil and shade on the hatching success of diamondback terrapins to determine whether nesting habitat could be increased by using dredged bottom *** year 1,unshaded nests in natural loamy-sand had the highest hatching success(55.2%),while nests in dredge soil produced no *** year 2,nests in unshaded loamy-sand had a hatching success of 85.3%,whereas those in dredge soil,aged 1 year,had a hatching success of 59.4%.Dredge soil improved as an incubation substrate after aging 1 year by the washout of *** temperatures were generally cool and produced mostly male *** dredge soil may provide suitable nesting substrates for diamondback terrapins if used after one year,and may be particularly beneficial if used for constructing islands that provide new nesting sites with reduced access of mammalian predators.

关键词： dredge estuarine turtles hatching success Malaclemys terrapin nesting ecology nest temperature nest substrate shade