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In-Silico Identification and Differential Analysis of Mitochondrial RNA Editing Events in Helianthus Genotypes/Species and Powdery Mildew Infected Variants

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American Journal of Plant Sciences 2023年第12期14卷 1464-1479页

作者： Duruvasula Sree Lekha kandasamy Ulaganathan Mulpuri Sujatha Centre for Plant Molecular biology Osmania University Hyderabad India ICAR-Indian Institute of Oilseeds Research Rajendranagar Hyderabad India

Sunflower is one of the most used commercial oilseed crops and suffers due to Powdery mildew. RNA sequence alteration occurs due to RNA editing which is a post transcriptional modification. It causes a deviation from the genomic DNA sequence resulting in RNA-DNA differences. Accurate study of RNA editing events in diverse species is possible by NGS based methods. Here, we performed RNA sequencing of 12 leaf transcriptomes, which include three genotypes of Helianthus annuus (2023b, TX16R and ID25), H. debilis, H. niveus, and H. praecox along with their respective powdery mildew pathogen infected variants and systematically analysed the mitochondrial RNA editing events using computational reference-based mapping approach. we discovered 687 editing sites, 220 editing events in the protein-coding regions, among all species and genotypes considered in this study. These included “C to U” and “U to C” RNA editing events. On further analysis, we observed that these editing events include 14 different types of amino acid changes that involve the creation of two stop codon events. The conserved editing sites identified were 247 accounting for ~36% of all the editing sites identified. This study provides a detailed picture of the Helianthus species’ mitochondrial RNA editing status. we have identified and characterized for the first time, genotype-specific, species-specific, and stress-specific RNA editing events which may be useful as a potential source for stress-responsive studies in the future.

关键词： Helianthus annuus RNA Editing RNA-seq H. niveus H. debilis H. praecox biotic Stress Powdery Mildew

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b-Chromatic Number of M[Cn],M[Pn],M[F1,n] and M[wn]

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Open Journal of Discrete Mathematics 2011年第2期1卷 85-88页

作者： Duraisamy Vijayalakshmi kandasamy Thilagavathi Narayanan Roopesh 不详

In this paper, we discuss about the b-colouring and b-chromatic number for middle graph of Cycle, Path, Fan graph and wheel graph denoted as M[Cn],M[Pn],M[F1,n] and M[wn] .

关键词： Chromatic Number b-Chromatic b-Colouring Middle Graph

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water Stress Responsive Differential Methylation of Organellar Genomes of Zea maysZ59

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American Journal of Plant Sciences 2020年第7期11卷 1077-1100页

作者： bhupathipalli Divya bhanu kandasamy Ulaganathan Arun K. Shanker Centre for Plant Molecular biology Osmania University Hyderabad India Central Research Institute for Dryland Agriculture Hyderabad India

DNA methylation is an important epigenetic change affecting gene expression in plants in both normal and stress conditions. The organelles, mitochondria and chloroplast play a significant role in sensing and initiating stress response. In this study, we report the methylation pattern in chloroplast and mitochondrial genomes in irrigated and water stressed conditions and its relationship with gene expression of a drought tolerant Zea mays cultivar, Z59. Whole genome bisulfite sequencing was done to analyze the pattern of methylation in both the conditions. Mapping of bisulfite reads to B73 reference of mitochondrial and chloroplast genomes showed hypomethylation in water stressed plants when compared to irrigated plants. Sliding window approach to the methylation count data showed highest peak at 419,800 to 420,800 bp region in mitochondria and at 36,900 to 37,900 bp region in chloroplast genomes in both samples. Annotation of the methylated genomes showed that, genes related to photosystem I & II in chloroplast and nad4 gene in mitochondria were hypo methylated in the water stressed sample. RNA-seq analysis of transcriptomics reads mapped to the same reference showed regulation of rps3, rps2A, ccmFC, atp1 and many uncharacterized genes in mitochondria and psbA, psbD, psbc, psaA, and atpA, genes in chloroplast.

关键词： Maize Drought Stress DNA Methylation Epigenetics Mitochondria Chloroplast RNA-Seq

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Synthesis, Growth, Crystal Structure and Characterization of the o-Toluidinium Picrate

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Journal of Crystallization Process and Technology 2013年第4期3卷 123-129页

作者： kandasamy Mohana Priyadarshini Angannan Chandramohan Thangarak Uma Devi Department of Chemistry Sri Ramakrishna Mission Vidyalaya College of Arts and Science Coimbatore India Department of Chemistry Sri Ramakrishna Mission Vidyalaya College of Arts and Science Coimbatore India Department of Physics Government Arts College for Women Pudukottai India.

A new organic charge transfer molecular complex salt of o-toluidinium picrate (OTP) was synthesised and the single crystals were grown by the slow solvent evaporation solution growth technique using methanol as a solvent at room temperature. Formation of the new crystal has been confirmed by single crystal X-ray diffraction (XRD) and NMR spectroscopic techniques. The crystal structure determined by single crystal X-ray diffraction indicates that both the cation and the anion are interlinked to each other by three types of intermolecular hydrogen bonds, namely N(4)-H(4A)···O(7), N(4)-H(4b)···O(5) and N(4)-H(4C)···O(7). The title compound (OTP) crystallizes in monoclinic crystal system with the centrosymmetric space group P21/c. Fourier transform infrared (FT IR) spectral analysis was used to confirm the presence of various functional groups in the grown crystal. The optical properties were analyzed by the UV-Vis-NIR and fluorescence emission studies.

关键词： Single Crystal Organic Molecules Solution Growth X-Ray Diffraction Characterization Nonlinear Optical

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Triggering the biocontrol of botrytis cinerea by Trichoderma harzianum through inhibition of pathogenicity and virulence related proteins

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Frontiers of Agricultural Science and Engineering 2018年第2期5卷 271-279页

作者： kandasamy SARAVANAKUMAR Zhixiang LU Hai XIA Meng wANG Jianan SUN Shaoqing wANG Qiang-qiang wANG Yaqian LI Jie CHEN School of Agriculture and biology/State Key Laboratory of Microbial Metabolism/Key Laboratory of Urban Agriculture (South) of the Ministry of Agriculture Shanghai Jiao Tong University

This study reports a strain of Trichoderma harzianum CCTCC-Sbw0162 with potential to enhance biocontrol activity against gray mold pathogen, botrytis cinerea, and with a pivotal role in tomato(Solanum esculentum) plant growth enhancement. A total of 254 Trichoderma isolates were screened by in vitro antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of b. *** in vitro antagonistic assay and greenhouse experiments indicated that T. harzianum CCTCC-Sbw0162 gave the highest inhibition rate(90.6%) and disease reduction(80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-Sbw0162 against b. cinerea, molecular docking was used to assess the interactions between CCTCC-Sbw0162-derived metabolites, and pathogencity and virulence related proteins of b. cinerea. The molecular docking results indicated that the combination of harzianopyridone,harzianolide and anthraquinone C derived from CCTCCSbw0162 could synergistically improve antifungal activity against b. cinerea through the inhibition/modification of pathogenicity and virulence related ***, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect T. harzianum-derived metabolites against the proteins of b. cinerea and their interactions.

关键词： anthraquinone botrytis cinerea harzianolide harzianopyridone molecular docking Trichoderma harzianum

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基于多参数MRI的SLIC-超体素方法对骨肉瘤新辅助化疗的疗效评价

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国际医学放射学杂志 2020年第4期 499-500页

作者： E.b.Kayal D.kandasamy R.Sharma M.C.Sharma S.bakhshi A.Mehndiratta 刘春明 Centre for biomedical Engineering Indian Institute of Technology Delhi

目的组织病理学检查(HPE)是目前评价肿瘤化疗反应的金标准,但只适用于手术后。本研究旨在开发一种无创的、基于影像学的有效方法,通过勾画、可视化和定量分

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Titanium-containing high entropy oxide(Ti-HEO):A redox expediting electrocatalyst towards lithium polysulfides for high performance Li-S batteries

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Nano Research Energy 2024年第3期3卷 39-49页

作者： Hassan Raza Junye Cheng Jingwei wang Subash kandasamy Guangping Zheng Guohua Chen Department of Materials Science Shenzhen MSU-BIT UniversityShenzhen 517182China Department of Mechanical Engineering Research Institute for Smart EnergyThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina School of Energy and Environment City University of Hong KongDat Chee AvenueKowloonHong Kong SARChina

Since lithium sulfur(Li-S)energy storage devices are anticipated to power portable gadgets and electric vehicles owing to their high energy density(2600 wh·kg^(-1));nevertheless,their usefulness is constrained by sluggish sulfur reaction kinetics and soluble lithium polysulfide(LPS)shuttling *** electrically conductive bifunctional electrocatalysts are urgently needed for Li-S batteries,and high-entropy oxide(HEO)is one of the most promising *** this work,we synthesize titanium-containing high entropy oxide(Ti-HEO)(TiFeNiCoMg)O with enhanced electrical conductivity through calcining metal-organic frameworks(MOF)templates at modest *** resulting single-phase Ti-HEO with high conductivity could facilitate chemical immobilization and rapid bidirectional conversion of *** a result,the Ti-HEO/S/Kb cathode(with 70 wt.%of sulfur)achieves an initial discharge capacity as high as~1375 mAh·g^(-1)at 0.1 C,and a low-capacity fade rate of 0.056%per cycle over 1000 cycles at 0.5 *** increased sulfur loading(~5.0 mg·cm^(-2)),the typical Li-S cell delivered a high initial discharge capacity of~607 mAh·g^(-1)at 0.2 C and showcased good cycling *** work provides better insight into the synthesis of catalytic Ti-containing HEOs with enhanced electrical conductivity,which are effective in simultaneously enhancing the LPS-conversion kinetics and reducing the LPS shuttling effect.

关键词： titanium containing high entropy oxide(Ti-HEOs) multi-metal-MOFs template method electrical conductivity lithium-sulfur batteries catalytic conversion

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