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The Influence of Culture Medium Type on Cellular Phenotype of Canine Adipose Derived Stem Cells

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Open Journal of Regenerative Medicine 2014年第1期3卷 28-37页

作者： Kristina M. kiefer G. Elizabeth Pluhar Michael G. Conzemius Timothy D. O’Brien Department of Veterinary Clinical Sciences College of Veterinary Medicine University of Minnesota St. Paul USA Department of Veterinary Population Medicine College of Veterinary Medicine University of Minnesota St. Paul USA

Canine adipose derived stem cells (ASCs) hold a great promise for the therapy of osteoarthritis in veterinary medicine. Current therapy is an autologous, stromal vascular fraction. Allogeneic ASCs provide many advantages, including efficient, cost-effective treatments while eliminating a surgical procedure in a diseased animal. Cultured ASCs can be expanded and characterized, allowing selection of desirable qualities. Use of allogeneic ASCs requires selection of a culture medium that provides consistent, desirable cellular products. The supplements within a medium can greatly influence cellular phenotypes. we hypothesized that medium type influenced cellular phenotype, allowing selection of a specified cellular product for clinical applications. we evaluated ASCs derived from adipose tissue of six dogs, assessing mRNA expression of proinflammatory: interleukin-1b, cyclooxygenase-2, and anti-inflammatory mediators: tissue inhibitor metalloproteinase-2 and interleukin -1 receptor antagonist, via quantitative RT-PCR prior to, and following culture in five cell culture media: basic cell growth medium (BGM), Keratinocyte N acetyl-L-cysteine supplemented (KNAC) medium, Multipotent Adult Progenitor Cell (MAPC) medium, serum free medium (SFM) and xeno-free medium. Major histocompatability complex I (MHCI), major histocompatability complex II (MHCII), CD44 and CD90 immunophenotypes were assessed via flow cytometry analysis. Tri-lineage differentiation (bone, adipose and cartilage tissue) was utilized to verify multipotency. SFM and xeno-free culture conditions did not produce cell expansion sufficient to assess phenotype. ASCs prior to culture had wide variability in all mediator levels, while culturing in the remaining conditions resulted in more predictable expression levels of inflammatory mediators, with a decrease in all levels. Cultured ASCs retained expression of cell surface markers MHCI, CD44 and CD90, while decreasing MHCII expression levels. KNAC and MAPC medium c

关键词： Canine Mesenchymal Stem Cells Adipose-Derived Stromal Cells Culture Conditions

瑞士驻沪总领事馆和德国驻上海总领事馆文化教育处的室内空间

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时代建筑 2003年第6期46卷 86-89页

作者： Pirjo kiefer 张晓春上海意迅装饰设计工程有限公司

瑞士总领事馆和德国驻沪领事馆文化教育处两个项目由上海意迅装饰设计工程有限公司(Shanghai laction Decoration Design & Engineering ***. )的建筑设计师Pirjo kiefer设计。两个项目的并置,可以看到设计师在处理室内不同吊顶高度条... 详细信息

瑞士总领事馆和德国驻沪领事馆文化教育处两个项目由上海意迅装饰设计工程有限公司(Shanghai laction Decoration Design & Engineering ***. )的建筑设计师Pirjo kiefer设计。两个项目的并置,可以看到设计师在处理室内不同吊顶高度条件限制情况下的不同设计方法。

关键词：室内空间文化教育领事馆上海德国瑞士建筑设计师设计工程

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纽伦堡

美术观察 2011年第4期 F0004-F0004页

作者： Anselm kiefer

Development of an antibody that neutralizes soluble IgE and eliminates IgE expressing B cells

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Cellular & Molecular Immunology 2016年第3期13卷 391-400页

作者： Andrew C. Nyborg Anna Zacco Rachel Ettinger M. Jack Borrok Jie Zhu Tom Martini Rob woods Christine kiefer Michael A. Bowen E. Suzanne Cohenz Ronald Herbst Herren wu Steven Coats Medlmmune LLC Gaithersburg MD USA and 2Medlmmune Ltd Milstein Building Granta Park Cambridge UK

Immunoglobulin E （IgE） plays a key role in allergic asthma and is a clinically validated target for monoclonal antibodies. Therapeutic anti-lgE antibodies block the interaction between IgE and the Fc epsilon （Fcε） receptor, which eliminates or minimizes the allergic phenotype but does not typically curtail the ongoing production of IgE by B cells. we generated high-affinity anti-lgE antibodies （MEDI4212） that have the potential to both neutralize soluble IgE and eliminate IgE-expressing B-cells through antibody-dependent cell-mediated cytotoxicity. MEDI4212 variants were generated that contain mutations in the Fc region of the antibody or alterations in fucosylation in order to enhance the antibody＇s affinity for FcyRIIla. All MEDI4212 variants bound to human IgE with affinities comparable to the wild-type （wT） antibody. Each variant was shown to inhibit the interaction between IgE and FcεRI, which translated into potent inhibition of FcyRI-mediated function responses. Importantly, all variants bound similarly to IgE at the surface of membrane IgE expressing cells. However, MEDI4212 variants demonstrated enhanced affinity for FcyRIIla including the polymorphic variants at position 158. The improvement in FcyRIIla binding led to increased effector function in cell based assays using both engineered cell lines and class switched human IgE B cells. Through its superior suppression of IgE, we anticipate that effector function enhanced MEDI4212 may be able to neutralize high levels of soluble IgE and provide increased long-term benefit by eliminating the IgE expressing B cells before they differentiate and become IgE secreting plasma cells.

关键词： antibody therapeutic anti-lgE asthma IgE monoclonal antibody

Inactivation and Mutation of Yeast Cells Induced by Heavy ion Beam

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IMP & HIRFL Annual Report 1994年 184-185页

作者： wei Zengquan H.D.Pross U.weidmaun J. kiefer and G.Lenz(Strahlenzentrum,Justus-Liebig-University,Giessen,Germany.GSI ,Germany.)

ＩｎａｃｔｉｖａｔｉｏｎａｎｄＭｕｔａｔｉｏｎｏｆＹｅａｓｔＣｅｌｌｓＩｎｄｕｃｅｄｂｙＨｅａｖｙｉｏｎＢｅａｍ￥ＷｅｉＺｅｎｇｑｕａｎ；Ｈ．Ｄ．Ｐｒｏｓｓ；Ｕ．Ｗｅｉｄｍａｕｎ；Ｊ．Ｋｉｅｆｅｒ... 详细信息

ＩｎａｃｔｉｖａｔｉｏｎａｎｄＭｕｔａｔｉｏｎｏｆＹｅａｓｔＣｅｌｌｓＩｎｄｕｃｅｄｂｙＨｅａｖｙｉｏｎＢｅａｍ￥ＷｅｉＺｅｎｇｑｕａｎ；Ｈ．Ｄ．Ｐｒｏｓｓ；Ｕ．Ｗｅｉｄｍａｕｎ；Ｊ．ＫｉｅｆｅｒａｎｄＧ．Ｌｅｎｚ（Ｓｔｒａｈｌｅ...

关键词： Mutation Yeast

皮肤黑色素瘤患者的淋巴结及皮下转移超声:一项前瞻性多中心研究结果

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世界核心医学期刊文摘（皮肤病学分册） 2006年第3期2卷 52-53页

作者： Blum A. Schmid-wendtnerM.-H. Mauss-kiefer V. 党倩丽 Private and Teaching Practice of Dermatology Seestrasse 3a DE-78464 Konstanz Germany Dr

Background: Ultrasound (sonography,B-mode sonography, ultrasonography) examination improves the sensitivity in more than 25% compared to the clinical palpation, especially after surgery on the regional lymph node area. Objective: To evaluate the distribution of metastases during follow-up in the draining lymph node areas from the scar of primary to regional lymph nodes (head and neck, supraclavicular, axilla, infraclavicular, groin) in patients with cutaneous melanoma with or without sentinel lymph node biopsy (SLNB) or former elective or consecutive complete lymph node dissection in case of positive sentinel lymph node (CLND). Methods: Prospective multicenter study of the Departments of Dermatology of the Universities of Homburg/Saar, Tü bingen and Munich (Germany) in which the distribution of lymph node and subcutaneous metastases were mapped from the scar of primary to the lymphatic drainage region in 53 melanoma patients (23women, 30 men; median age: 64 years; median tumor thickness: 1.99 mm) with known primary, visible lymph nodes or subcutaneous metastases proven by ultrasound and histopathology during the follow-up. Results: Especially in the axilla, infraclavicular region and groin the metastases were not limited to the anatomic lymph node regions. In 5 patients (9.4% ) (4 of them were in stage IV) lymph node metastases were not located in the corresponding lymph node area. 32 patients without former SLNB had a time range between melanoma excision and lymph node metastases of 31 months (median), 21 patients with SLNB had 18 months (p with positive SLNB the time range was 17 months, in 10 patients with negative SLNB 21 months (pwith CLNDthe time range was 31 months and in 21 patients without CLND 18 months (pw significant changes which shoul

关键词：前瞻性多中心研究皮肤黑色素瘤局部淋巴结皮下转移超声患者前哨淋巴结活检淋巴结清扫术外科手术后淋巴引流区

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顿悟

2010年1月至2015年3月美国因缺氧和吸入烃类气体/蒸气引起的石油天然气开采工人急性死亡调查

作者： (美)查尔斯·基弗(Charles kiefer) (美)马尔科姆·康斯特布尔(Malcolm Constable)

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上海预防医学 2017年第12期29卷 975-977页

作者： Robert J.Harrison Kyla Retzer Michael J.Kosnett Michael Hodgson Todd Jordan Sophia Ridl Max kiefer 杨凤 Division of Occupational and Environmental Medicine$ University of California San Francisco National Institute for Occupational Safety and Healtli Western States Division CDC Division of Clinical Pharmacology and Toxicology University of Colorado School of Medicine 4. Department of Environmental and Occupational Health Colorado School of Public Health Office of Occupational Medicine and Nursing Occupational Safety and Health Administration$ Washingtons DC Health Response Team Occupational Safety and Healtii Administration Salt Lake City Utah 不详

2013年,1名来自旧金山加州大学的职业医师向美国疾病预防控制中心（CDC）国家职业安全与卫生研究院（National Institute for Occupational Safety and Health,NIOSH）和职业安全与健康管理局（Occupational Safety and Health Administration,OSHA）报告美国西部2名石油天然气开采工人的死亡病例,引起死亡的原因可能是由于工人打开油气储罐的舱门时,暴露在烃类气体/蒸气（hydrocarbon gases and vapors,HGVs）中且合并缺氧。

关键词：石油天然气天然气开采 NIOSH 烃类气体

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约翰·多恩：《封圣》——抒情诗叙事学分析

玉溪师范学院学报 2019年第4期35卷 37-42页

作者：詹斯·基弗谭君强(译) 汉堡大学跨学科叙事学研究中心德国汉堡20146 云南大学文学院云南昆明650091

17世纪英国玄学派诗人约翰·多恩的《封圣》是一首著名的抒情诗。诗歌以自身故事的抒情人对他的交流对象,即他的受述者讲述的形式,展现了抒情人对他爱情的自信。他以各种方式为他的爱情辩护,确信即便他的爱情现在不被接受,但在未来,他... 详细信息

17世纪英国玄学派诗人约翰·多恩的《封圣》是一首著名的抒情诗。诗歌以自身故事的抒情人对他的交流对象,即他的受述者讲述的形式,展现了抒情人对他爱情的自信。他以各种方式为他的爱情辩护,确信即便他的爱情现在不被接受,但在未来,他将以自己与所爱的人的爱情载入圣者名册而成为他人的楷模。在这首17世纪的诗歌中已经包含对现代概念的爱情的清晰表达。

关键词：约翰·多恩《封圣》抒情人脚本事件性