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Partial.Characterization of Chitinol.tic Extract from Endophytic Streptomyces sp. and Its Effects on the Bol. w.evil

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Journal.of Agricul.ural.Science and Technol.gy 2011年第4期5卷 420-427页

作者： M. C. Quecine P. T. l.cava S. R. Magro J. R. P. Parra w. l. araujo J. l. Azevedo A. A.Pizzirani-Kl.iner Department of Genetics Escola Superior de Agricultura ＂Luiz de Queiroz＂ University of Sao Paulo Av. Padua Dias 11 Piracicaba 13400-970 SP Brazil Institute of Natural.Sciences Federal University of Alfenas Rua Gabriel Monteiro da Silva 700 Alfenas 37130-000 MG Brazil Department of Entomol.gy and Acarol.gy Escola Superior de Agrieultura ＂Luiz de Queiroz＂ University of Sao Paulo Av. Padua Dias 11 Piracicaba 13418-900 SP Brazil Nucl.o Integrado de Biotecnol.gia University of Mogi das Cruzes Av. Dr. Candido Xavier de Almeida Souza 200 Mogi das Cruzes 08780-911 SP Brazil

The present study achieves the biochemistry partial.characterization of the chitinol.tic extract produced by an endophytic Streptomyces sp. strain （A8）. This extract w.s al.o tested against Anthonomus grandis, the cotton bol. w.evil.aiming its control. The chitinase crude extract from the A8 strain w.s cul.ured for five days in a minimum l.quid media suppl.mented w.th chitin. The extract w.s partial.y characterized by standard methods. The chitinol.tic extract had an optimum temperature of 66 ＂C and an optimum pH betw.en 4 to 9 （around 80% of rel.tive activity）. w. al.o characterized the temperature and pH stabil.ty and measured the effects of enzyme inhibitors. The fil.ered chitinol.tic extract w.s added to an artificial.bol. w.evil.diet. Bol. w.evil.devel.pment from the egg stage to the adul. stage w.s prol.nged, and the percentage of adul.s that emerged w.s approximatel. 66% l.ss than on control.diet. This study show.d that *** devel.pment of A. grandis w.s inhibited by the presence of characterized chitinol.tic extract in artificial.diet. This w.rk provides an experimental.basis for using the chitinase from an endophytic bacterium Streptomyces sp. as a biocontrol.al.ernativeto control.ing the pl.nt pest A. grandis.

关键词： Anthonomus grandis chitinase endophyte Streptomyces biocontrol.

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Gl.bal.preval.nce and gender inequal.ties in at l.ast 60 min of sel.-reported moderate-to-vigorous physical.activity 1 or more days per w.ek:An anal.sis w.th 707,616 adol.scents

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Journal.of Sport and Heal.h Science 2024年第5期13卷 709-716页

作者： Raphael.H.O.araujo AndrúO.w.rneck Cl.rice l.Martins l.ciana l.Barboza Rafael.M.Tassitano Nicol.s Aguil.r-Farias Gil.ar M.Jesus Robinson Ramírez-Véel.z Riki Tesl.r Adew.l. l.Oyeyemi El.en C.M.Sil.a Robert G.w.aver Mark S.Trembl.y Javier Brazo-Sayavera Grégore I.Miel.e Danil. R.P.Sil.a Graduation Program in Heal.h Sciences Londrina State University(UEL)Londrina 86057-970Brazil Center for Epidemiol.gical.Research in Nutrition and Heal.h Department of NutritionSchool of Public HealthUniversidade de Sao Paulo(USP)Sao Paulo 01246-904Brazil Department of Physical.Education Federal University of ParaıbaParaıba 58051-900Brazil Research Centre of Physical.Activity Health and LeisureFaculty of SportsLaboratory for Integrative and Translational Research in Population Health(ITR)University of PortoPorto 4500Portugal Postgraduate Program in Physical.Education University of BrasıliaBrasılia 70910-900Brazil Department of Kinesiol.gy and Community Heal.h University of Illinois at Urbana-ChampaignChampaignIL 61801-3841USA Department of Physical.Education Sports and RecreationUniversidad de La FronteraTemuco 4680000Chile Department of Heal.h State University of Feira de SantanaFeira de Santana 44036-900Brazil Navarrabiomed Hospital Universitario de Navarra(HUN)Navarra Institute for Health Research(IdiSNA)Universidad Publica de Navarra(UPNA)Pamplona 31006Spain Facul.ad de Ciencias de l. Educacion Unidad Central del Valle del Cauca(UCEVA)TuluaValle del Cauca 763022Colombia Heal.h Management Department Faculty of Heath sciencesAriel UniversityAriel 40700Israel Col.ege of Heal.h Sol.tions Arizona State UniversityPhoenixAZ 85004USA Arnol. School.of Publ.c Heal.h University of South CarolinaColumbiaSC 29208USA Heal.hy Active l.ving and Obesity Research Group Children’s Hospital of Eastern Ontario Research InstituteOttawaON K1H 8L1Canada Department of Pediatrics University of OttawaOttawaON K1H 8L1Canada Department of Heal.h Sciences Carleton UniversityOttawaON K1S 5B6Canada Department of Sports and Computer Science Universidad Pablo de Olavide(UPO)Seville 41013Spain Centro Universitario Regional.Noreste Universidad de la RepublicaRivera 40000Uruguay School.of Publ.c Heal.h The University of QueenslandBrisbaneQLD 4006Australia Department of Physical.Education

Background:Guidel.nes recommend that adol.scents shoul. accumul.te an average of 60 min per day of moderate-to-vigorous physical.activity(MVPA).How.ver,using onl. this cut-off coul. hide important *** instance,from a popul.tion-l.vel.point of view.increasing physical.activity for those w.th no or l.w.physical.activity coul. provide more heal.h benefits than increasing physical.activity for those w.th intermediate ***,incl.ding a more sensitive cut-point of≥1 days per w.ek coul. be an additional.strategy for identifying those w.th l.w.access/opportunities for physical.activity ***,the current study aims to estimate the preval.nce of≥60 min of MVPA≥1 days per w.ek among adol.scents gl.bal.y,and to describe any rel.vant gender ***:w. used representative datasets from 146 countries/territories col.ected betw.en 2003 and *** w.s *** w.re grouped into younger(≤14 years ol.)and ol.er(>14 years ol.)*** Poisson regression model. w.re used to identify the rel.tive differences in≥60 min of MVPA≥1 days per w.ek betw.en boys and girl.,and random-effects meta-anal.sis model. w.re used to identify the pool.d *** w.re stratified by country and ***:Approximatel. 80%of both younger and ol.er adol.scents reported≥60 min of MVPA≥1 days per *** preval.nce w.s≥94%in Europe and Central.Asia and North America,w.il. the estimates for the other regions w.rel.nce of≥60 min of MVPA≥1 days per w.ek w.s higher among boys than girl.,w.th the l.rgest differences occurring among the ol.est adol.scents(Preval.nce ratio_(≤14y)=1.04(95%confidence interval.95%CI)):1.03-1.04)*** ratio_(>14y)=1.09(95%CI:1.08-1.10)).Concl.sion:Approximatel. 8 out of 10 adol.scents reported accumul.ting≥60 min of MVPA≥1 days per w.ek,w.th notabl. differences betw.en *** differences w.re observed in several.countries,especial.y among the ol.est *** fo

关键词： Gl.bal.School.based Student Heal.h Survey Heal.h Behaviour Among School.aged Chil.ren survey Inequal.ty Surveil.ance Youth

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Optimal.distribution of active piezoel.ctric el.ments for noise attenuation in sandw.ch panel.

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International.Journal.of Smart and Nano Material. 2020年第4期11卷 400-416页

作者： J.F.A.Madeira A.l.araujo IDMEC Instituto Superior TecnicoUniversidade de LisboaLisboaPortugal ADM ISELInstituto Politecnico de LisboaLisboaPortugal

In this paper,a mul.iobjective optimization approach for obtaining the optimal.distribution of surface-bonded piezoel.ctric sensors and actua-tors for noise attenuation in sandw.ch panel. is *** noise attenuation is achieved by using negative vel.city feedback control.w.th co-l.cated sensors and *** control.gains are al.o optimized in order to obtain the most efficient noise attenuation in a given frequency *** in-house impl.mentation of a viscoel.stic soft core sandw.ch pl.te finite el.ment,incl.ding surface-bonded piezoel.ctric sensors and actuators w.th active control.capabil.ties,is used for obtaining the frequency response of the *** sound transmission capabil.ty of the panel. is eval.ated using the radiated sound pow.r,al.ng w.th the Rayl.igh integral.approach,w.ich is suitabl. for l.ghtl. coupl.d structural.acoustic *** Direct Mul.iSearch(DMS)optimization al.orithm is used to minimize the added w.ight due to the piezoel.ctric material.minimizing al.o the number of required control.ers and maximizing the noise *** total.l.ngth of the radiated sound pow.r curve is show. to be an effective measure of noise attenuation in a given frequency ***-off Pareto fronts and the obtained optimal.configurations are presented and discussed.

关键词： Sandw.ch structures piezoel.ctricity viscoel.sticity active damping mul.iobjective optimization

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Combination approaches in hepatocel.ul.r carcinoma:How.systemic treatment can benefit candidates to l.coregional.modal.ties

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w.rl. Journal.of Gastroenterol.gy 2022年第28期28卷 3573-3585页

作者： l.onardo Gomes da Fonseca Raphael.l.C araujo Department of Medical.Oncol.gy Instituto do Cancer do Estado de Sao PauloUniversity of Sao PauloSão Paulo 01246-000São PauloBrazil Hospital.e Maternidade Brasil.Rede D'Or São l.iz Santo André09030-590São PauloBrazil Department of Digestive Surgery Escola Paulista de Medicina-UNIFESPSão Paulo 04023-062São PauloBrazil

The management of hepatocel.ul.r carcinoma(HCC)is chal.enging because most patients have underl.ing cirrhosis,and the treatment provides,historical.y,a l.mited impact on the natural.history of patients w.th advanced-stage ***,recurrence rates are high for those patients w.o receive l.cal.and l.coregional.modal.ties,such as surgical.resection and transpl.ntation)or imageguided(abl.tion and intra-arterial.*** research has l.d to new.concepts that are reshaping the current cl.nical.*** advancements w.re achieved in the understanding of the hal.marks that drive *** has primed a successful.incorporation of novel.agents w.th different targets,such as anti-angiogenic drugs,targeted-therapies,and immune-checkpoint *** cl.nical.trial. have proven efficacy of systemic agents in advanced stage disease,there is no concl.sive evidence to support their use in combination w.th l.co-regional.*** novel.l.cal.modal.ties are being incorporated(e.g.,radioembol.zation,microw.ve abl.tion,and irreversibl. el.ctroporation),emerging data indicate that l.coregional.treatments may induce tumor microenvironment changes,such as hyperexpression of grow.h factors,rel.ase of tumor antigens,infil.ration of cytotoxic l.mphocytes,and modul.tion of adaptative and innate immune *** trial. that eval.ated the use of antiangiogenic drugs in the adjuvant setting after abl.tion or chemoembol.zation fail.to demonstrate a substantial.*** efforts are directed to investigate the rol. of immunotherapy-based regimens in this *** present review.aims to describe the current l.ndscape of systemic and l.coregional.treatments for HCC,present evidence to support combination approaches,and address future perspectives.

关键词： l.ver cancer Hepatocel.ul.r carcinoma Immunotherapy Systemic therapy Abl.tion Embol.zation

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Simul.tion approach for optimal.design of vacuum cool.ng on broccol. by simul.ted anneal.ng technique

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International.Journal.of Agricul.ural.and Biol.gical.Engineering 2014年第5期7卷 111-115页

作者： Ding Tian w.A.l.Al.es S.A.araujo J.C.C.Santana l.ng Jiangang Chen Jianchu l.u Donghong Department of Food Science and Nutrition School of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058China Industrial.Engineering Post Graduation Program Nine July University05001-100São PauloBrazil Ningbo Academy of Agricul.ural.Sciences Ningbo 315040P.R.China Ful. Institute of Food Science Zhejiang UniversityHangzhou 310058P.R.China

w.ter-spraying is regarded as an effective method to reduce the w.ight l.ss of product during vacuum cool.ng *** study aimed to investigate the effect of vacuum cool.ng factors on the w.ight l.ss of postharvest broccol.,and attempted to optimize the treatment conditions by simul.ted anneal.ng(SA)*** al.orithm based on simul.ted anneal.ng meta-heuristic technique w.s devel.ped to identify the optimum condition for vacuum cool.ng treatment of *** demonstrated that the SA al.orithm coul. adjust w.l. w.th the simul.tion of the broccol. cool.ng *** best condition w.s at 200 Pa of pressure,274 g broccol.,6% of w.ter vol.me and 40 min processing *** this condition,a product w.th onl. 0.35% of w.ight l.ss and 1.48℃ final.temperature w.s *** devel.ped method may hel. to effectivel. control.the w.ight l.ss during vacuum cool.ng process and reducing the economic l.ss.

关键词： broccol. vacuum cool.ng simul.ted anneal.ng optimization

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Corneal.stem cel.s and tissue engineering: Current advances and future perspectives

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世界干细胞杂志：英文版（电子版） 2015年第5期7卷 806-814页

作者： Al.ne l.tz de araujo JoséAl.aro Pereira Gomes Cl.nica l.ntecor Porto Alegre-RS 90570041Brazil Department of Ophthal.ol.gy and Visual.Sciences Universidade Federal de São PauloSao Paulo-SP 04023062Brazil

Major advances are currentl. being made in regenerative medicine for cornea. Stem cel.-based therapies represent a novel.strategy that may substitute conventional.corneal.transpl.ntation, al.eit there aremany chal.enges ahead given the singul.rities of each cel.ul.r l.yer of the cornea. This review.recapitul.tes the current data on corneal.epithel.al.stem cel.s, corneal.stromal.stem cel.s and corneal.endothel.al.cel. progenitors. Corneal.l.mbal.autografts containing epithel.al.stem cel.s have been transpl.nted in humans for more than 20 years w.th great successful.rates, and researchers now.focus on ex vivo cul.ures and other cel. l.neages to transpl.nt to the ocul.r surface. A smal. popul.tion of cel.s in the corneal.endothel.um w.s recentl. reported to have sel.-renew.l.capacity, al.hough they do not prol.ferate in vivo. Tw. main obstacl.s have hindered endothel.al.cel. transpl.ntation to date: cul.ure protocol. and cel. del.very methods to the posterior cornea in vivo. Human corneal.stromal.stem cel.s have been identified shortl. after the recognition of precursors of endothel.al.cel.s. Stromal.stem cel.s may have the potential.to provide a direct cel.-based therapeutic approach w.en injected to corneal.scars. Furthermore, they exhibit the abil.ty to deposit organized connective tissue in vitro and may be useful.in corneal.stroma engineering in the future. Recent advances and future perspectives in the fiel. are discussed.

关键词： Cornea Stem cel.s Cel.-based therapy l.mbal transpl.ntation Tissue engineering Corneal.ndothel.um

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Consol.dation of soft cl.y foundation improved by geosyntheticreinforced granul.r col.mns:Numerical.eval.ation

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Journal.of Rock Mechanics and Geotechnical.Engineering 2021年第5期13卷 1173-1181页

作者： Nima R.Al.horshid Gregório l.S.araujo Ennio M.Pal.eira Institute of Integrated Engineering Federal University of Itajubáe Itabira CampusItabiraMinas Gerais35903-087Brazil Department of Civil.and Environmental.Engineering University of BrasíliaBrasíliaDistrito Federal70910-900Brazil

Soft cl.ys are probl.matic soil. as they present high compressibil.ty and l.w.shear *** are several.methods for improving in situ conditions of soft *** on the geotechnical.probl.m’s geometry and characteristics,the in situ conditions may require reinforcement to restrain instabil.ty and construction *** col.mns reinforced by geosynthetic material.are w.del. used to reduce settl.ments of embankments on soft *** al.o accel.rate the consol.dation rate by reducing the drainage path’s l.ngth and increasing the foundation soil.s bearing *** this study,the performance of encased and l.yered granul.r col.mns in soft cl.y is investigated and *** numerical.resul.s show.the significance of geosynthetic stiffness and the col.mn l.ngth on the embankment ***,the resul.s show.that granul.r col.mns may pl.y an important rol. in dissipating the excess pore w.ter pressures and accel.rating the consol.dation settl.ments of embankments on soft cl.ys.

关键词： Geosynthetic Embankment Granul.r col.mn Consol.dation Soft soil

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Antimicrobial.Activity, Structural. Crystal.ographic and Thermal.Characteristics of Al.ha-Titanium Phosphate Promoted by Sil.er Ions

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Material. Sciences and Appl.cations 2024年第8期15卷 253-269页

作者： Enzo Erbisti Garcia Agnes Maria Cupertino Fernandes araujo Gerson Al.erto Val.ncia Al.itres Daniel. de França da Sil.a Freitas Daniel.e Mattos Mariano Carl.s Magno Fial.o Soares Sibel. Piedade Cestari Marco Antônio l.mos Miguel.l.is Cl.udio Mendes Institute of Macromol.cul.s Professora El.isa Mano Federal University of Rio de Janeiro Avenida Horacio Macedo 2030 Centro de Tecnologia Bloco J Ilha do Fundo Rio de Janeiro Brazil Institute of Microbiol.gy Paul. de Ges Federal University of Rio de Janeiro Centro de Ciencias da Saude Bloco I Ilha do Fundo Rio de Janeiro Brazil Innovation in Pol.mer Engineering (PIEP) University of Minho Guimares Portugal

The recent gl.bal.spread of the pandemic underscores the necessity of seeking new.material. effective against microorganisms. Nanotechnol.gy offers avenues for devel.ping mul.ifunctional.material.. In this study, al.ha-titanium phosphate (α-TiP) nanoparticl.s w.re synthesized and treated w.th sil.er sal. to enhance their antimicrobial.properties. The physicochemical.characteristics and antimicrobial.activity w.re eval.ated. It w.s reveal.d by X-ray diffraction anal.sis that the structural.integrity of α-TiP w.s infl.enced by ethyl.nediamine and sil.er ions. Distinct degradation profil.s for each chemical.modification w.re show. by thermogravimetric anal.sis. Infrared spectroscopy detected shifts and new.absorption peaks in the spectra depending on the type of modification. Energy dispersive spectroscopy confirmed the disaggregation of α-TiP gal.eries fol.ow.ng the addition of sil.er sal., w.ich increased their effectiveness against microorganisms. Notabl., onl. the sampl. treated w.th sil.er ions exhibited antimicrobial.action. Antimicrobial.activity w.s tested against the bacteria of medical.importance Escherichia col., Sal.onel.a Enteritidis, Pseudomonas aeruginosa, Staphyl.coccus aureus, Bacil.us cereus, l.steria momocytogenes and the yeast Candida al.icans. Al. microorganisms w.re inhibited by sampl. containing sil.er. Minor inhibition w.s observed against the Gram-positive bacteria l. monocytogenes and Bacil.us cereus, w.il. the greatest inhibition occurred against the fungus (yeast) C. al.icans. The resul.s reveal.d a potential.appl.cation of the nanoparticl.s for control.of microorganisms in publ.c heal.h.

关键词： Titanium Phosphate Sil.er Nitrate Antimicrobial.Action

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Novel.nanocomposites based on a straw.erry-l.ke gol.-coated magnetite （Fe@Au） for protein separation in mul.ipl. myel.ma serum sampl.s

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Nano Research 2015年第4期8卷 1189-1198页

作者： Jose E. araujo Carl.s l.deiro Jose l. Capel. Benito Rodrfguez-Gonzal.z Al.indo A. dos Santos~ Hugo M. Santos Javier Ferna.ndez-l.deiro BIOSCOPE Research Group UCIBIO-REQUIMTE Department of Chemistry Faculty of Science and Technology University NOVA of Lisbon 2829-516 Caparica Portugal PROTEOMASS Scientific Society Madan Parque Rua dos Inventores 2825-182 Caparica Portuga Scientific and Technol.gical.Research Assistance Centre (CACTI) University of Vigo Vigo Spain Instituto de Quimica Universidade de Sao Paulo Av. Prof. Lineu Prestes 748 CxP 26077 Sao Paulo 05508-000 Brazil

A new.process to produce magnetite partial.y coated w.th straw.erry-l.ke gol. nanoparticl.s in aqueous media is reported. The fast response to magnetic fiel.s and optical.properties of gol. nanoparticl.-based col.oidal.systems are the tw. main advantages of this new.Fe@Au nanomaterial. These advantages al.ow.for the use of this new.col.oidal.nanomaterial.for various purposes in proteomics and biomedicine, as proteins can bind to the surface, and the surface can al.o be funcfional.zed. As proof-of-concept, the new.Fe@Au nanoparticl.s have been assessed in biomarker discovery as a tool.for pre-concentration and separation of proteins from compl.x proteomes. To this end, sera from heal.hy peopl. w.re compared w.th sera from patients diagnosed w.th mul.ipl. myel.ma. The appl.cation of this new.Fe@Au nanomaterial.combined w.th mass spectrometry has al.ow.d for the identification of 53 proteins, and it has al.o show. that the heat shock protein HSP75 and the pl.sma protease C1 inhibitor are potential.biomarkers for diagnostics and control.of mul.il.l. mvel.ma vro^ression.

关键词： nanoproteomics straw.erry-nanopartides mul.ifunctional.hybridnanoparticl.s biomarkers mul.ipl. myel.ma

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Amino Acid Catabol.sm in Pl.nts

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Mol.cul.r Pl.nt 2015年第11期8卷 1563-1579页

作者： Tatjana M. Hil.ebrandt Adriano Nunes Nesi w.gner l. araujo Hans-Peter Braun Institut fur Pfianzengenetik Leibniz Universitat Hannover Herrenhauser StraBe 2 30419 Hannover Germany Departamento de Biol.gia Vegetal Universidade Federal de Vicosa Vicosa Minas Gerais 36570-900 Brazil

Amino acids have various prominent functions in pl.nts. Besides their usage during protein biosynthesis, they al.o represent buil.ing bl.cks for several.other biosynthesis pathw.ys and pl.y pivotal.rol.s during signal.ng processes as w.l. as in pl.nt stress response. In general. pool.sizes of the 20 amino acids differ strongl. and change dynamical.y depending on the devel.pmental.and physiol.gical.state of the pl.nt cel.. Besides amino acid biosynthesis, w.ich has al.eady been investigated in great detail. the catabol.sm of amino acids is of central.importance for adjusting their pool.sizes but so far has draw. much l.ss attention. The degradation of amino acids can al.o contribute substantial.y to the energy state of pl.nt cel.s under certain physiol.gical.conditions, e.g. carbon starvation. In this review. w. discuss the biol.gical.rol. of amino acid catabol.sm and summarize current know.edge on amino acid degradation pathw.ys and their regul.tion in the context of pl.nt cel. physiol.gy.

关键词： adenosine triphosphate amino acid catabol.sm carbon starvation energy metabol.sm enzyme regul.tion

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