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Recent Developments in Metallic Degradable Micromotors for Biomedical and Environmental Remediation Applications

Nano-Micro Letters 2024年第3期16卷 1-35页

作者： Sourav Dutta Seungmin Noh Roger Sanchis Gual Xiangzhong Chen Salvador Pané Bradley j.Nelson Hongsoo Choi Department of Robotics and Mechatronics Engineering Daegu Gyeongbuk Institute of Science and Technology(DGIST)Daegu 42988Republic of Korea DGIST‑ETH Microrobotics Research Center DGISTDaegu 42988Republic of Korea Multi‑Scale Robotics Lab Institute of Robotics and Intelligent SystemsETH Zurich8092 ZurichSwitzerland Institute of Optoelectronics State Key Laboratory of Photovoltaic Science and TechnologyShanghai Frontiers Science Research Base of Intelligent Optoelectronics and PerceptionFudan UniversityShanghai 200433People’s Republic of China

Synthetic micromotor has gained substantial attention in biomedicine and environmental ***-based degradable micromotor composed of magnesium(Mg),zinc(Zn),and iron(Fe)have promise due to their nontoxic fuel-free propulsion,favorable biocompatibility,and safe excretion of degradation products Recent advances in degradable metallic micromotor have show. their fast movement in complex biological media,efficient cargo delivery and favorable biocompatibility.A notew.rthy number of degradable metal-based micromotors employ bubble propulsion,utilizing w.ter as fuel to generate hydrogen *** novel feature has proj.cted degradable metallic micromotors for active in vivo drug delivery *** addition,understanding the degradation mechanism of these micromotors is also a key parameter for their design and *** propulsion efficiency and life span govern the overall performance of a degradable metallic *** w. review.the design and recent advancements of metallic degradable ***,w. describe the controlled degradation,efficient in vivo drug delivery,and built-in acid neutralization capabilities of degradable micromotors w.th versatile biomedical ***,w. discuss micromotors’efficacy in detecting and destroying environmental ***,w. address the limitations and future research directions of degradable metallic micromotors.

关键词： Magnesium Zinc Iron Biodegradable microrobot Biomedical Environmental

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Spectro-Microscopy of Living Plant Cells

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Molecular Plant 2012年第1期5卷 14-26页

作者： Klaus Hatter Alfred j. Meixner Frank Schleifenbaum Center for Plant Molecular Biology （ZMBP） Plant Physiology and Biophysical Chemistry University of Tubingen Auf der Morgenstelle 1 72076 Tuebingen Germany Institute for Physical and Theoretical Chemistry （IPTC） Nano-Optics University of Tubingen Auf der Morgenstelle 8 72076 Tubingen Germany

Spectro-microscopy, a combination of fluorescence microscopy w.th spatially resolved spectroscopic techni- ques, provides new.and exciting tools for functional cell biology in living organisms. This review.focuses on recent devel- opments in spectro-microscopic applications for the investigation of living plant cells in their native tissue context. The application of spectro-microscopic methods led to the recent discovery of a fast signal response pathw.y for the brassi- nosteroide receptor bri1 in the plasma membrane of living plant cells. Moreover, the competence of different plant cell types to respond to environmental or endogenous stimuli w.s determined in vivo by correlation analysis of different optical and spectroscopic readouts such as fluorescence lifetime （FLT）. Furthermore, a new.spectro-microscopic technique, fluorescence intensity decay shape analysis microscopy （FIDSAM）, has been developed. FIDSAM is capable of imaging low. expressed fluorophore-tagged proteins at high spatial resolution and precludes the misinterpretation of autofluorescence artifacts. In addition, FIDSAM provides a very effective and sensitive tool on the basis of F6rster resonance energy transfer （FRET） for the qualitative and quantitative determination of protein-protein interaction. Finally, w. report on the quan- titative analysis of the photosystem I and II （PSI/PSII） ratio in the chloroplasts of living Arabidopsis plants at room tem- perature, using high-resolution, spatially resolved fluorescence spectroscopy. w.th this technique, it w.s not only possible to measure PSI/PSII ratios, but also to demonstrate the differential competence of w.ld-type and carbohydrate-deficient plants to adapt the PSI/PSII ratio to different light conditions. In summary, the information content of standard microscopic images is extended by several dimensions by the use of spectro-microscopic approaches. Therefore, novel cell physiolog- ical and molecular topics can be addressed and valuable insigh

关键词： Fluorescence microscopy FLIM FRET photosystems protein-protein interaction FIDSAM.

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INFLUENCE OF SLIP VELOCITY ON BLOOD FLOw. THROUGH AN ARTERY w.TH PERMEABLE w.LL： A THEORETICAL STUDY

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International j.urnal of Biomathematics 2012年第5期5卷 147-166页

作者： A. SINHA j. C. MISRA School of Medical Science and Technologyindian Institute of Technology Kharagpur-721302 India Department of 1Vlathematics Institute of Technical Education and ResearchSiksha 0 Anusandhan University Bhubaneswar India

A theoretical investigation concerning the influence of slip velocity on the flow.of blood through an artery having its w.ll permeable has been carried out. Here blood is treated as a homogeneous New.onian fluid. The flow.is characterized by three parameters： /3 the ratio of radius to length of the arterial segment, Re the characteristic Reynolds number associated w.th the pressure outside the arterial segment and c the filtration coe^cient. The problem has been solved by the use of a perturbation technique, e is considered to be very small, ensuring the validity of the perturbation method. The computed numerical results are presented graphically to depict the variations in velocity, volumetric flow.rate, w.ll shear stress and flow.resistance.

关键词： Slip velocity permeable artery new.onian fluid.

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Immunophilin-like FKBP42/Tw.STED Dw.RF1 Interacts w.th the Receptor Kinase bri1 to Regulate Brassinosteroid Signaling in Arabidopsis

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Molecular Plant 2016年第4期9卷 593-600页

作者： j.thamas Chaiw.non Veder j. Garcia Heather Cartw.ight Ying Sun Zhi-Yong w.ng Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA Department of Botany Center of Excellence in Environment and Plant Physiology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand present address: Institute of Molecular Cell Biology Hebei Key Laboratory of Molecular and Cellular Biology Hebei Normal University Shijiazhuang 050024 China

Mutation of the immunophilin-like FK506-binding protein Tw.STED Dw.RF1 （FKBP42/Tw.1） causes dw.rf and tw.sted-organ phenotypes in Arabidopsis. How.ver, the function of FKBP42 is not fully understood at the molecular level. Using genetic, physiological, and immunological experiments, w. show.here that FKBP42/Tw.1 is necessary for brassinosteroid （BR） signal transduction. The tw.l mutant show.d reduced BR sensitivity in grow.h responses and activation of the BZR1 transcription factor. How.ver, tw.l show.d normal responses to an inhibitor of BIN2/GSK3, suggesting that tw.l has a defect upstream of BIN2 in the BR signaling pathw.y. In vitro and in vivo assays show.d that Tw.1 interacts physically w.th the kinase domains of the BR receptor kinases bri1 and BAK1. Tw.1 is not required for normal localization of bri1-GFP to the plasma membrane or for activation of the flagellin receptor kinase FLS2. Our results suggest that FKBP42/Tw.1 plays a specific role in the activation of bri1 receptor kinase.

关键词： brassinosteroid signaling bri1 FKBP42

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Particle size distribution and shape control of Au nanoparticles used for particle gun

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Frontiers of Chemical Science and Engineering 2013年第1期7卷 60-64页

作者： S. Kida M. Ichij. j. w.tanabe I. Hirasaw. Graduate School of Advanced Sciencel Engineering Waseda University Tokyo 169-8555 Japan

Au nanoparticles are expected for the media to transfer genes into plants. How.ver, the control of particle size distribution （PSD） and shape of Au nanoparticles is too difficult to design and prepare particles w.th suitable quality for the gene supporting media. Reduction crystal- lization experiments w.re performed in aqueous solution in order to clarify the effect of feeding conditions such as feeding profile, feeding rate, and feeding amount on PSD and shape of Au nanopartieles. Ascorbic acid （AsA） w.s selected as a reducing agent because it is safe for plants. Au particles of 50nm, 50-200nm, and 150-400nm w.re obtained in batch operation, single-j.t, and double-j.t, respectively. Moreover, in single-j.t and double-j.t, the mean size of the obtained Au particles increases w.th the decrease of feeding rate or the increase of feeding amount. It is concluded that PSD of Au nanoparticles can be controlled in the range of 50-400 nm by changing feeding conditions of AsA and HAuCl4 aqueous solution.

关键词： reduction crystallization particle size distri- bution gene transferring media

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Cloning of murine bri3 gene and study on its function for inducing cell death

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Chinese Science Bulletin 2002年第5期47卷 375-378,441页

作者： Lin, LZ Yu, H Ying, MY Tang, j.Zhou, w.Zhao, SY Li, CB Fudan Univ Sch Life Sci Inst Genet State Key Lab Genet Engn Shanghai 200433 Peoples R China

To understand the molecular mechanism of TNFα effects, the cDNA of murine bri3 gene w.s cloned from the total RNA of murine brain endothelial cells (bEnd.3) treated w.th hTNFa by using the suppression subtractive hybridization (SSH) and the RT-PCR method. The fusion expression vector harbouring bri3 gene and enhanced green fluorescence protein (EGFP) thus obtained w.re designated as pEGFP/I3. Then pEGFP/I3 w.s transiently transfected into L929 cells and the fusion protein EGFP/I3 w.s localized in cytoplasm. It is found that the expression of EGFP/I3 could induce cell death in L929 cells detected by TUNEL method and flow.cytometry. And the overexpression of Bcl-2 in L929 cells can block cell death induced by EGFP/I3, indicating that murine bri3 gene might related to the TNFα mediated cytotoxicity.

关键词： tumor necrosis factor α bri3 fusion protein cell death.

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Human RhCG Ammonia Conduction Mechanism

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Computational Molecular Bioscience 2020年第3期10卷 81-94页

作者： Henry j. Hoyhtya Hanna j. Koster Maggie M. Christiansen Ugur Akgun Physics Department Coe College Cedar Rapids USA

The structural differences betw.en E. coli AmtB and the human RhCG as w.ll as the Rh50 from Nitrosomonas europaea suggest different ammonia conduction mechanisms for the AmtB and the Rh proteins. This study investigates the mechanism differences by using molecular dynamics simulations on RhCG and Rh50NE structures. Unlike AmtB the Rh proteins lack the aromatic cage at the bottom of the periplasmic vestibule. This report establishes the periplasmic Glu residue as the NH+4 binding site for Rh proteins, and the tw. Phe residues at the entrance of the pore as the NH3 binding site. The NH+4 molecule pushed by another ammonium releases one of its protons on its w.y to the phenyl gate. This study also discovers that, unlike AmtB, the Rh protein pores allow.w.ter molecules, w.ich eventually facilitates the NH3 conduction from periplasm to cytoplasm.

关键词： Rh Proteins RhCG Rh50 Rh50NE Ammonia Channel

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Periodic surface functional group density on graphene via laserinduced substrate patterning at Si/SiO2 interface

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Nano Research 2020年第9期13卷 2332-2339页

作者： Karolina A.Drogow.ka-Horna Inam Mirza Alvaro Rodriguez Petr Kovaricek j.raj.Sladek Thibault j.-Y.Derrien Mindaugas Gedvilas Gediminas Raciukaitis Otakar Frank Nadezhda M.Bulgakova Martin Kalbac j.Heyrovsky Institute of Physical Chemistry of the Czech Academy of Sciences Dolejskova 2155/318223 PragueCzech Republic HiLASE Centre Institute of Physics of the Czech Academy of SciencesZa Radnict 82825241 Dolni BrezanyCzech Republic Faculty of Nuclear Sciences and Physical Engineering Czech Technical University in PragueBrehova 78/711519Czech Republic Center for Physical Sciences and Technology Savanoriu Ave.231LT-023000VilniusLithuania

Controlling the spatial distribution of functional groups on tw.-dimensional(2D)materials on a micrometer scale and below.represents a fascinating opportunity to achieve anisotropic(opto)electronic properties of these *** patterns of covalent functionalization can lead to pericxj.c potentials in the monolayer;how.ver,creating such superstructures is very ***,w. describe an original approach to the periodic functionalization of graphene induced by substrate patterning using a pulsed ***-induced periodic surface structures(LIPSS)are produced on silicon w.fers w.th thermally-grow. oxide *** irradiation conditions for the formation of UPSS confined at the SiO2/Si interface have been *** imprint their periodicity to the reactivity of the monolayer graphene placed on the substrate via modulation of its local doping *** method is clean,straightforw.rd and scalable w.th high spatial resolution.

关键词： tw.-dimensional(2D)materials graphene functionalization laser-induced periodic surface structures(LIPSS) periodic patterns Raman spectroscopy atomic force microscopy(AFM)

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Spatial Prediction of Soil Aggregate Stability and Aggregate-Associated Organic Carbon Content at the Catchment Scale Using Geostatistical Techniques

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Pedosphere 2011年第3期21卷 389-399页

作者： j.MOHAMMADI M.H.MOTAGHIAN Department of Soil Science Agriculture CollegeShahrekord University

The association of organic carbon w.th secondary particles （aggregates） results in its storage and retention in soil. A study w.s carried out at a catchment covering about 92 km2 to predict spatial variability of soil w.ter-stable aggregates （w.A）, mean w.ight diameter （Mw.） of aggregates and organic carbon （OC） content in macro.- （〉 2 mm）, meso- （1-2 mm）, and micro-aggregate （〈 1 mm） fractions, using geostatistical methods. One hundred and eleven soil samples w.re eSlleeted at the 0 10cm depth and fractionated into macro-, meso-, and mlcro-aggregates by w.t sieving. The OC content w.s determined for each fraction. A greater percentage of w.ter-stable aggregates w.s found for micro-aggregates, follow.d by meso-aggregates. Aggregate OC content w.s greatest in meso-aggregates （9 g kg-1）, follow.d by micro-aggregates （7 g kg-1）, w.ile the least OC content w.s found in macro-aggregates （3 g kg-1）. Although a significant effect （P = 0.000） of aggregate size on aggregate OC content w.s found, how.ver, our findings did not support the model of aggregate hierarchy. Land use had a significant effect （P = 0.073） on aggregate OC content. The coefficients of variation （CVs） for OC contents associated w.th each aggregate fraction indicated macro-aggregates as the most variable （CV = 71%）. Among the aggregate fractions, the micro-aggregate fraction had a low.r CV value of 27%. macro-aggregates to 84% for micro-aggregates. Geostatistical analysis differences in their spatial patterns in both magnitude and space at variance for most aggregate-associated properties w.s low.r than 45%. The mean content of w.A ranged from 15% for show.d that the measured soil variables exhibited each aggregate size fraction. The relative nugget The range value for the variogram of w.ter-stable aggregates w.s almost similar （about 3 km） for the three studied aggregate size classes. The range value for the variogram of aggregate-associated OC contents ranged from about 3 km for macro-aggregates to about

关键词： kriging organic matter variogram w.ter-stable aggregates

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Online Fuzzy Logic Base Algorithm for Electricity Price Prediction

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j.urnal of Electronic Science and Technology 2017年第4期15卷 371-376页

作者： Moamin j.Albayed A.R.Al-Ali Mostafa Shaaban Computer Science and Engineering Department American University of Sharjah Sharjah 2666 UAE Electrical Engineering Department American University of Sharjah Sharjah 2666 UAE

This paper presents a w.b-based system to predict the electricity *** proposed system captures the geographical location,w.ather forecast,and oil price for one w.ek *** captured parameters are fed to a fuzzy-logic-based algorithm to calculate electric energy *** on predicted electricity prices,consumers can turn ON/OFF or reschedule operations of their home appliances to reduce their electricity *** proposed algorithm w.s developed and hosted in a utility server(U-server).On the consumer side,a home gatew.y(H-gatew.y),and a monitoring and control system w.s designed,built,and tested by using a single chip microcontroller.

关键词： Electricity tariff fuzzy logic home automation smart energy

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