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Culture of Neural Stem Cells in Calcium-alginate Microbeads

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生物医学工程学杂志 2005年第S1期22卷 105-106页

作者： Li-Song YAO~1 Tian-Qing LIU~(1△) Dan GE~1 Xue-Hu MA~1 Zhan-Feng CUI~2 1(Stem Cell and Tissue Engineering Laboratory, Dalian university of Technology, Dalian 116024, China)2(department of Engineering Science, oxford university, parks road, oxford ox1 3PJ, uk) Stem Cell and Tissue Engineering Laboratory Dalian University of Technology Dalian 116024 China department of Engineering Science Oxford University Parks Road Oxford OX1 3 PJ UK

1 IntroductionRecent research show. that neural stem cells may play an important role in the nerve injury reparation and nerve disease treatment. The shortage of the source and the number of NSCs, how.ver, is the main challenge for its clinic application. In this situation, expansion of NSCs in large scale and culture in three dimensional environment are very w.rth of exploration. Notablely, the shear stress existed in bioreactors can cause serious cell injury especially for the shear sensitive cells like NSCs.

关键词： NSCs Ca

The Effects of AFGP Addition and Removing Protocol of CPA on Vitrification Cryopreservation of Osteoblasts

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生物医学工程学杂志 2005年第S1期22卷 81-82页

作者： w.n-Xia FAN~1 Xue-Hu MA~(1Δ) Tian-Qing LIU~1 Zhan-Feng CUI~(1,2)1(Stem Cells and Tissue Engineering Lab., Dalian university of Technology, Dalian 116023, China)2(department of Engineering Science, oxford university, parks road, oxford ox1 3PJ, uk)

Effects of CPA Loading Temperature and Removal Protocols on MNC Vitrification

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生物医学工程学杂志 2005年第S1期22卷 87-88页

作者： Yang JIN~1 Xue-Hu MA~1 Dan GE~1 Tian-Qing LIU~1 Zhan-Feng CUI~(1,2)1(Stem Cell and Tissue Engineering Laboratory, Dalian university of Technology, Dalian 116024, China)2(department of Engineering Science, oxford university, parks road, oxford ox1 3PJ, uk)

Adding and Removal of CPAs in Osseous Tissue for Cryopreservation

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生物医学工程学杂志 2005年第S1期22卷 89-90页

作者： Xue-Hu MA~1 w.i BAI~1 Yang LIU~1 Dan GE~1 Zhan-Feng CUI~(1,2) Tian-Qing LIU~11(Stem Cell & Tissue Engineering Laboratory, Dalian university of Technology, Dalian 116024,China)2(department of Engineering Science, oxford university, parks road, oxford ox1 3PJ, uk)

Three-dimensional Fabrication of Bio-derived Bone Compounded w.th Osteoblasts Transfected by Green Fluorescent Protein

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生物医学工程学杂志 2005年第S1期22卷 93-94页

作者： Ke-Dong SONG~1 Tian-Qing LIU~(1Δ) Zhi-Ming YANG~3Xiang-Qin LI~1 Zhan-Feng CUI~2 Xue-Hu MA~11(Stem Cell and Tissue Engineering Laboratory, Dalian university of Technology, Dalian 116024, China) 2(department of Engineering Science, oxford university, parks road, oxford ox1 3PJ, uk)3(Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotecherapy, w.st China Hospital,Sichuan university, Chengdu 610041, China)

新型旋转壁式生物反应器内三维组织工程骨的构建

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生物化学与生物物理进展 2004年第11期31卷 996-1005页

作者：宋克东刘天庆李香琴崔占峰葛丹孙相玉马学虎大连理工大学精细化工国家重点实验室干细胞与组织工程研究室大连116024 department of Engineering Science Oxford UniversityParks RoadOxford OX1 3PJUK

利用微载体悬浮培养法将成骨细胞在旋转壁式生物反应器内进行大规模扩增 ,并检测细胞的组织形态和生物功能 .然后以此作为种子细胞 ,分别以 2× 10 6个 /ml和 1× 10 6个 /ml两种密度接种到支架材料上 ,于旋转壁式生物反应器 (Rw.)内进... 详细信息

利用微载体悬浮培养法将成骨细胞在旋转壁式生物反应器内进行大规模扩增 ,并检测细胞的组织形态和生物功能 .然后以此作为种子细胞 ,分别以 2× 10 6个 /ml和 1× 10 6个 /ml两种密度接种到支架材料上 ,于旋转壁式生物反应器 (Rw.)内进行三维组织工程骨的构建 .并将所构建的骨组织分别进行倒置显微镜 (invertedmicroscope)、扫描电镜 (SEM )、碱性磷酸酶 (ALP)、矿化结构和AO/EB双重荧光染色等生物学性能检测 ,以及对培养过程的营养物质代谢情况进行监控和分析 .结果表明 ,在Rw.中培养的骨组织生长良好 ,分泌大量胶原纤维 ,并有矿化基质和新骨样组织形成 .由上述结果可断定 ,通过Rw.内部流体对流所产生的应力刺激 ,可提高成骨细胞碱性磷酸酶的活性表达 ,并加速矿化结节的形成 ,从而完成成骨细胞的快速增殖与分化以及工程化组织的三维构建 .

关键词：组织工程骨成骨细胞生物反应器支架材料细胞培养

低氧环境中微囊化成骨细胞支持造血干/祖细胞扩增

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生物化学与生物物理进展 2010年第7期37卷 754-761页

作者：宋克东刘天庆赵国峰武爽方美云石芳鑫朱丽丽马学虎崔占峰大连理工大学干细胞与组织工程研发中心大连116024 大连医科大学附属第一医院血液科大连116011 大连医科大学附属第一医院妇产科大连116011 department of Engineering Science Oxford UniversityParks RoadOxford OX13PJUK

在模拟骨髓造血壁龛(hematopoietic niche)的氧分压条件下,探讨微囊化成骨细胞(osteoblasts,OB)对脐血造血干/祖细胞(HSPC)体外扩增的支持和调控机理.分离培养人髂骨OB,采用聚电解质络合法将第3代的OB以密度为8×105ml包埋在直径为0.5m... 详细信息

在模拟骨髓造血壁龛(hematopoietic niche)的氧分压条件下,探讨微囊化成骨细胞(osteoblasts,OB)对脐血造血干/祖细胞(HSPC)体外扩增的支持和调控机理.分离培养人髂骨OB,采用聚电解质络合法将第3代的OB以密度为8×105ml包埋在直径为0.5mm的明胶-海藻酸钠-壳聚糖(GAC)微胶珠中.将微珠+造血干/祖细胞(A′组)、造血干/祖细胞(B′组)及微珠(C′组)置于6孔板,在5%氧分压下进行培养.同时在20%常氧条件下设置同样分组培养作为对照(A,B,C).通过流式细胞分析和半固体细胞集落培养,观察比较各培养体系中造血干/祖细胞的扩增,并检测体系内白血病抑制因子(LIF)和白介素-6(IL-6)的含量变化以探讨作用机理.经过倒置相差显微镜观察,人成骨细胞在微珠中分散均匀,生长状态良好.微珠内部有丰富的孔道供营养物质传递,有大量造血干/祖细胞弱黏附于微珠表面.经过7天的培养,A′、B′、A、B四组造血细胞的扩增倍数分别为(49.0±4.6),(3.3±0.5),(17.7±1.2)和(1.9±0.2).A′、B′、A组的CD34+细胞分别扩增了(87.6±8.3),(2.2±0.3)和(14.9±1.0)倍,B组则出现下降.A′、B′、A、B四组CFU-Cs集落扩增倍数分别为(9.8±0.8),(3.5±0.4),(6.9±0.7)和(2.6±0.2).低氧共培养体系比常氧共培养体系和非共培养体系对造血干/祖细胞的扩增有更大的促进作用.A′、B′、C′中IL-6和LIF含量明显高于对应的A、B、C组,与扩增倍数的差异相对应.微囊化成骨细胞对造血干/祖细胞扩增有明显的促进作用,5%氧分压接近体内造血壁龛氧环境,在此环境中成骨细胞分泌细胞因子量增多并通过其对造血干/祖细胞的扩增进行调节.

关键词：低氧造血干/祖细胞微胶珠成骨细胞共培养

Tw. EPISODES OF MONAZITE CRYSTALLIZATION DURING METAMORPHISM AND CRUSTAL MELTING IN THE EVEREST REGION OF THE NEPALESE HIMALAYA

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地学前缘 2000年第S1期7卷 27-27页

作者： Robert L. Simpson 1, Randall R. Parrish 2, Mike P. Searle 1, David J. w.ters 1[w.5,5”BX](1 department of Earth Sciences, oxford university, parks road, oxford ox1 3PR, uk 2 NERC Isotope Geosciences Laboratory, Keyw.rth, Nottingham NG12 5GG, uk)

New.monazite U\|Pb geochronological data from the Everest region suggest that 20～25Ma elapsed betw.en the initial India—Asia collision and kyanite\|sillimanite grade metamorphism. Our results indicate a tw.\|phase metamorphic history, w.th peak Barrovian metamorphism at (32 2±0 4)Ma and a later high\|temperature, low.|pressure event (620℃, 400MPa) at (22 7±0 2)Ma.. Emplacement and crystallization of the Everest granite subsequently occurred at 20 5～21 3Ma. The monazite crystallization ages that differ by 10Ma are recorded in tw. structurally adjacent rocks of different lithology, w.ich have the same post collisional p—T history.. Scanning electron microscopy reveals that the younger monazite is elaborately shaped and grew.in close association w.th apatite at grain boundaries and triple junctions, suggesting that grow.h w.s stimulated by a change in the fluid regime. The older monazite is euhedral, is not associated w.th apatite, and is commonly armoured w.thin silicate minerals. During the low.|pressure metamorphic event, the armouring protected the older monazites, and a lack of excess apatite in this sample prevented new.grow.h. Textural relationships suggest that apatite is one of the necessary monazite\|producing reactants, and spots w.thin monazite that are rich in Ca, Fe, Al and Si suggest that allanite acted as a preexisting rare earth element host. w. propose a simplified reaction for monazite crystallization based on this evidence.

关键词： monazite crystallization metamorphism crustal melting Everest region Nepalese Himalaya

Large-scale Expansion of SD Rat’s Osteoblasts in a Novel Rotating w.ll Vessel Bioreactor

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Large-scale Expansion of SD Rat’s Osteoblasts in a Novel Ro...