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α1-COP modulates plasmodesmata function through sphingolipid enzyme regulation

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Journal of Integrative Plant Biology 2024年第8期66卷 1639-1657页

作者： Arya Bagus Boedi Iswanto Minh Huy vu Jong Cheol Shon Ritesh Kumar Shuwei Wu Hobin Kang Da-Ran kim Geon Hui Son Woe Yoen kim Youn-Sig Kwak Kwang Hyeon Liu Sang Hee kim Jae-Yean kim Division of Applied Life Science(BK21 FOUR Program) Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 52828Korea College of Pharmacy and Research Institute of Pharmaceutical Sciences Kyungpook National UniversityDaegu 702-701Korea Departement of Plant Medicine Gyeongsang National UniversityJinju 52828Korea Division of Life Science Gyeongsang National UniversityJinju 52828Korea

Callose,aβ-1,3-glucan plant cell wall polymer,regulates symplasmic channel size at plasmodesmata(PD)and plays a crucial role in a variety of plant ***,elucidating the molecular mechanism of PD callose homeostasis is *** screened and identified an Arabidopsis mutant plant with excessive callose deposition at PD and found that the mutated gene wasα1-COP,a member of the coat protein I(COPI)coatomer *** report that loss of function ofα1-COP elevates the callose accumulation at PD by affecting subcellular protein localization of callose degradation enzyme Pd *** process is linked to the functions of ERH1,an inositol phosphoryl ceramide synthase,and glucosylceramide synthase through physical interactions with theα1-COP ***,the loss of function ofα1-COP alters the subcellular localization of ERH1 and GCS proteins,resulting in a reduction of Glc Cers and Glc HCers molecules,which are key sphingolipid(SL)species for lipid raft *** findings suggest thatα1-COP protein,together with SL modifiers controlling lipid raft compositions,regulates the subcellular localization of GPI-anchored PDBG2 proteins,and hence the callose turnover at PD and symplasmic movement of *** findings provide the first key clue to link the COPI-mediated intracellular trafficking pathway to the callose-mediated intercellular signaling pathway through PD.

关键词： callose coatomer proteins membrane-bound vesicle plasmodesmata sphingolipid enzymes

An Efficient Differential Evolution for Truss Sizing Optimization Using AdaBoost Classifier

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Computer Modeling in Engineering & Sciences 2023年第1期134卷 429-458页

作者： Tran-Hieu Nguyen Anh-tuan vu Hanoi University of Civil Engineering Hanoi100000Vietnam

Design constraints verification is the most computationally expensive task in evolutionary structural optimization due to a large number of structural analyses thatmust be *** a surrogatemodel to approximate the behavior of structures instead of the exact structural analyses is a possible solution to tackle this ***,most existing surrogate models have been designed based on regression *** paper proposes a novel method,called CaDE,which adopts a machine learning classification technique for enhancing the performance of the Differential Evolution(DE)*** proposed method is separated into two *** the first optimization stage,the original DE is implemented as usual,but all individuals produced in this phase are stored as inputs of the training *** on design constraints verification,these individuals are labeled as“safe”or“unsafe”and their labels are saved as outputs of the training *** collecting enough data,an AdaBoost model is trained to evaluate the safety state of *** model is then used in the second stage to preliminarily assess new individuals,and unpromising ones are rejected without checking design *** method reduces unnecessary structural analyses,thereby shortens the optimization *** benchmark truss sizing optimization problems are solved using the proposed method to demonstrate its *** obtained results show that the CaDE finds good optimal designs with less structural analyses in comparison with the original DE and four other DE *** reduction rate of five examples ranges from 18 to over 50%.Moreover,the proposed method is applied to a real-size transmission tower design problem to exhibit its applicability in practice.

关键词： Structural optimization machine learning surrogate model differential evolution AdaBoost classifier

Effects of Serum Concentration, Synchronization Time and Confluence on the Cell-Cycle Synchronization Efficiency of Goat Fibroblasts

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Open Journal of Animal Sciences 2024年第3期14卷 194-203页

作者： Van Khanh Nguyen Huong Thu Thi vu Au Thi Hoang Yen kim Thi Pham Giang Thi Thanh Nhan Hung Phu Lai Lan Doan Pham Key Laboratory of Animal Cell Biotechnology National Institute of Animal Science Hanoi Vietnam

This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.

关键词： Goat Fibroblast Cycle Synchronisation Serum Concentration Synchronization Time Confluence

A Neural-network-based Approach to Study the Energy-optimal Hovering Wing Kinematics of a Bionic Hawkmoth Model

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Journal of Bionic Engineering 2019年第5期16卷 904-915页

作者： Anh tuan Nguyen Ngoc Doan Tran Thanh Trung vu Thanh Dong Pham Quoc Tru vu Jae-Hung Han Faculty of Aerospace Engineering Le Quy Don Technical University236 Hoang Quoc VietVietnam Office of International Cooperation Le Quy Don Technical University236 Hoang Quoc VietVietnam Department ofAerospace Engineerings Korea Advanced Institute of Science and Technology Daejeon 34141Republic ofKorea

This paper presents the application of an artificial neural network to develop an approach to determine and study the energy-optimal wing kinematics of a hovering bionic hawkmoth model.A three-layered artificial neural network is used for the rapid prediction of the unsteady aerodynamic force acting on the wings and the required *** this artificial network is integrated into genetic and simplex algorithms,the running time of the optimization process is reduced *** validity of this new approach is confirmed in a comparison with a conventional method using an aerodynamic model based on an extended unsteady vortex-lattice method for a sinu soidal wing kinematics *** studying the obtained results,it is found that actual hawkmoths do not hover under an energy-optimal ***,by tilting the stroke plane and lowering the wing positions,they can compromise and expend some energy to enhance their maneuverability and the stability of their flight.

关键词： optimal hovering wing kinematics artificial neural network insect flight genetic algorithm unsteady vortex-lattice method bionics

A fully automated framework for helicopter rotor blades design and analysis including aerodynamics,structure, and manufacturing

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Chinese Journal of Aeronautics 2016年第6期29卷 1602-1617页

作者： Ngoc Anh vu Jae Woo Lee tuan Phuong Nam Le Song Thanh Thao Nguyen Division of Computational Mechatronics Institute for Computational Science Ton Duc Thang University Faculty of Electrical & Electronics Engineering Ton Duc Thang University Department of Aerospace Information Engineering Konkuk University Faculty of Mechanical Engineering Industrial University of Ho Chi Minh City Department of Aerospace Engineering Ho Chi Minh City University of Technology Vietnam National University-Ho Chi Minh City

This study describes an integrated framework in which basic aerospace engineering aspects(performance, aerodynamics, and structure) and practical aspects(configuration visualization and manufacturing) are coupled and considered in one fully automated design optimization of rotor blades. A number of codes are developed to robustly perform estimation of helicopter configuration from sizing, performance analysis, trim analysis, to rotor blades configuration representation. These codes are then integrated with a two-dimensional airfoil analysis tool to fully design rotor blades configuration including rotor planform and airfoil shape for optimal aerodynamics in both hover and forward flights. A modular structure design methodology is developed for realistic composite rotor blades with a sophisticated cross-sectional geometry. A D-spar cross-sectional structure is chosen as a baseline. The framework is able to analyze all realistic inner configurations including thicknesses of D-spar, skin, web, number and ply angles of layers of each composite part,and materials. A number of codes and commercial software(ANSYS, Gridgen, VABS, Pre VABS,etc.) are implemented to automate the structural analysis from aerodynamic data processing to sectional properties and stress analysis. An integrated model for manufacturing cost estimation ofcomposite rotor blades developed at the Aerodynamic Analysis and Design Laboratory(AADL),Aerospace Information Engineering Department, Konkuk University is integrated into the framework to provide a rapid and dynamic feedback to configuration design. The integration of three modules has constructed a framework where the size of a helicopter, aerodynamic performance analysis, structure analysis, and manufacturing cost estimation could be quickly investigated. All aspects of a rotor blade including planform, airfoil shape, and inner structure are considered in a multidisciplinary design optimization without an exception of critical configuration.

关键词： Design optimization Helicopter rotor blades Integrated framework Modular structure design methodology Perform estimation

Piaoac Granites Related W-Sn Mineralization, Northern Vietnam: Evidences from Geochemistry, Zircon Geochronology and Hf Isotopes

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Journal of Earth Science 2019年第1期30卷 52-69页

作者： tuan Anh Nguyen Xiaoyong Yang Hien vu Thi Lei Liu Insung Lee School of Earth and Space Sciences University of Science and Technology of China Institute of Geological Sciences Vietnam Academy of Science and Technology(VAST) Department of Geology Hanoi University of Mining and Geology School of Earth and Environmental Sciences Seoul National University

Piaoac granites exposed in the Cao Bang region, northern Vietnam, are S-type granite, which are associated with W-Sn-Mo-Be-F mineralization. Zircon U-Pb ages, major and trace elements, mineral chemical and Hf isotopic compositions of the W-Sn-bearing granites from the Piaoac District have been investigated in detail. LA-ICP-MS U-Pb dating of zircon grains from these granites yielded ages of 82.5±2.3 and 82±1.8 Ma, representing an episode of Late Cretaceous magmatic event. These granites are characterized by high peraluminous and have typical S-type geochemical signatures with high SiO_2(72.37 wt.%–73.07 wt.%), high A/CNK values(1.61–1.65) and Al_2O_3(14.4 wt.%–15 wt.%). They are enriched in Rb, U, K, Th, Ta and Pb and display pronounced negative Ba, Sr, Nb, Ti and Eu(Eu/Eu*=0.19–0.24) anomalies. The high degree of fractional crystallization is characterized by low Rb, Sr, Ba and Eu concentrations with high ratios of La/Sm and Eu/Eu*. Zircon grains show εHf(t) values from-9.69 to-0.9 and the corresponding TDM2 range from 1.2 to 1.7 Ga, indicating that these granites could be derived from the Proterozoic basement rocks with minor input from mantle-derived magmas. The calculation of Fe^(3+) and Fe^(2+) of biotites indicates a low oxygen fugacity condition(log fO_2 ranging from 10-17 to 10-18 bars, below MH), which is favorable for the W-Sn mineralization. Tungsten and tin have been enriched in granitic magmas through fractionation, and low oxygen fugacity conditions have promoted the accumulation and transportation of W-Sn in the hydrothermal fluids, leading to deposition of mineral phases. The geochemical data suggest that Piaoac granites formed in an extensional setting related with the Late Cretaceous magmatism occurring large-scale lithospheric extensional in South China Block.

关键词： geochemistry zircon U-Pb age Hf isotope Piaoac granite W-Sn mineralization Northern Vietnam

Study of vertically ascending flight of a hawkmoth model

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Acta Mechanica Sinica 2020年第5期36卷 1031-1045,I0002页

作者： Anh tuan Nguyen vu Dan Thanh Le The Hung Tran V.N.Due Van Binh Phung Faculty of Aerospace Engineering Le Quy Don Technical UniversityHanoiVietnam Institute of Theoretical and Applied Research.Duy Tan University HanoiVietnam Division of Construction Computation Institute for Computational ScienceTon Due Thang UniversityHo Chi Minh CityVietnam Faculty of Civil Engineering Ton Due Thang UniversityHo Chi Minh CityVietnam

This paper provides insight into the wing kinematics,the power requirement and the dynamic stability characteristics of a hawkmoth model in vertically ascending *** wing kinematics of the hawkmoth model is obtained based on the minimum required power *** optimization process is conducted using genetic and simplex algorithms that are coupled with an artificial neural network to rapidly predict the aerodynamic force and required *** training data for the neural network are generated from an unsteady vortex-lattice *** to hover,the results in this study show the larger flapping frequency and the smaller rotation amplitude of the hawkmoth wing kinematics in ascending ***,more power is required when the ascending speed *** conducting a dynamic modal analysis based on a cycle-average approach,the certain effect of the ascending speed on the modal structures of the hawkmoth model was observed.

关键词： Hawkmoth Ascending flapping flight-Genetic algorithm Artificial neural network Flight dynamic stability

Optimal Wing Rotation Angle by the Unsteady Blade Element Theory for Maximum Translational Force Generation in Insect-mimicking Flapping-wing Micro Air Vehicle

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Journal of Bionic Engineering 2016年第2期13卷 261-270页

作者： Loan Thi kim Au Hoang vu Phan Hoon Cheol Park Department of Advanced Technology Fusion Konkuk University Seoul 143-701 South Korea

This paper provides a parametric study to obtain the optimal wing rotation angle for the generation of maximum transla- tional force in an insect-mimicking Flapping-Wing Micro Air Vehicle （FWMAV） during hovering. The blade element theory and momentum theory were combined to obtain the equation from which the translational aerodynamic force could be esti- mated. This equation was converted into a non-dimensional form, so that the effect of normalized parameters on the thrust coefficient could be analyzed. The research showed that the thrust coefficient for a given wing section depends on two factors, the rotation angle of the wing section and the ratio of the chord to the travel distance of the wing section in one flapping cycle. For each ratio that we investigated, we could arrive at an optimal rotation angle corresponding to a maximum thrust coefficient. This study may be able to provide guidance for the FWMAV design.

关键词： FWMAV optimal rotation angle parametric study blade element theory momentum theory

Comparison of Aerodynamic Forces and Moments Calculated by Three-dimensional Unsteady Blade Element Theory and Computational Fluid Dynamics

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Journal of Bionic Engineering 2017年第4期14卷 746-758页

作者： Loan Thi kim Au Hoang vu Phan Hoon Cheol Park Department of Advanced Technology Fusion and Artificial Muscle Research Center Konkuk University Seoul Korea

In previous work, we modified blade element theory by implementing three-dimensional wing kinematics and modeled the unsteady aerodynamic effects by adding the added mass and rotational forces. This method is referred to as Unsteady Blade Element Theory （UBET）. A comparison between UBET and Computational Fluid Dynamics （CFD） for flapping wings with high flapping frequencies （〉30 Hz） could not be found in literature survey. In this paper, UBET that considers the movement of pressure center in pitching-moment estimation was validated using the CFD method. We investigated three three-dimensional （3D） wing kinematics that produce negative, zero, and positive aerodynamic pitching moments. For all cases, the instantaneous aerodynamic forces and pitching moments estimated via UBET and CFD showed similar trends. The differences in average vertical forces and pitching moments about the center of gravity were about 10% and 12%, respectively. Therefore, UBET is proven to reasonably estimate the aerodynamic forces and pitching moment for flight dynamic study of FW-MAV. However, the differences in average wing drags and pitching moments about the feather axis were more than 20%. Since study of aerodynamic power requires reasonable estimation of wing drag and pitching moment about the feather axis, UBET needs further im- provement for hilzher accuracy.

关键词： blade element theory unsteady computational fluid dynamics flapping wings biomimetic