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世界核心医学期刊文摘（眼科学分册） 2005年第10期1卷 20-20页

作者： Funding M. vorum h. Nex E. Eh.ers N. 韩静 Department of Oph.h.lmology U'rhus University Hospital N?rrebrogade 44 8000 U'rhus C Denmark

Purpose: To investigate th. presence and concentration of α1-antitrypsin in aqueous h.mour at th. time of corneal rejection and to compare results obtained from patients with.reversible and irreversible rejection. Meth.ds: Samples of aq ueous h.mour were obtained from 17 patients with.acute corneal endoth.lial allog raft rejection. Th. presence of α1-antitrypsin in aqueous h.mour was confirmed by immunoblotting and measured employing a sandwich.ELISA. Total protein concen trations in aqueous h.mour were measured using Bradford’s meth.d. Th. outcome o f corneal rejection episodes was determined 1 month.after diagnosing corneal rej ection and described as reversible or irreversible rejection. Results: α1-anti trypsin was detected in aqueous h.mour. Patients with.reversible rejection h.d s ignificantly h.gh.r α1-antitrypsin concentration th.n patients with.irreversib le rejection (p=0.044). Th.re was no significant difference in total protein con centrations (p=0.745), and no correlation was found between α1-antitrypsin and total protein concentrations (p=0.368). Conclusions: α1-antitrypsin in aqueou s h.mour seems to signal a favourable outcome of corneal rejection. Th. possible mech.nism is discussed.

关键词：抗胰蛋白酶移植物排斥反应不可逆性免疫印迹法总蛋白预后良好 Bradford 无显著性差异

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急性角膜排斥患者的房水蛋白组学分析

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世界核心医学期刊文摘（眼科学分册） 2005年第8期1卷 10-10页

作者： Funding M. vorum h. h.noréB. 陈立军 Department of Oph.h.lmology úrhus University Hospital Nφrebrogade 44 8000 úrhus C Denmark

Purpose: To compare th. basic proteomic composition of aqueous h.mour (Ah. fro m patients with.corneal rejection (patients) with.Ah.from patients with.cataract (controls). Meth.ds: Aqueous h.mour was analysed for total protein concentratio n using Bradford’s meth.d and for protein composition using two-dimensional (2 D) gel electroph.resis. Image analysis was used to detect protein spots in 2D ge ls th.t were increased by more th.n factor 2 in patients as compared with.contro ls. Increased spots were identified by immunoblotting and mass spectrometry. Res ults: Aqueous h.mour from patients contained significantly h.gh.r total protein concentration th.n did Ah.from controls. A total of 31 spots were significantly increased in 2D gels from patients. Th. spots were derived from albumin, α1-an titrypsin, apolipoprotein J, cytokeratin type II, serin proteinase inh.bitor and transth.retin. After correction of spot volumes by total protein concentrations , 10 spots derived from albumin, cytokeratin type II and α1-antitrypsin remain ed significantly increased. Conclusion: Th. proteomic composition of Ah.differed significantly between patients and controls. Th. identified proteins suggest th.at th. ch.nges in Ah.are due to at least th.ee different mech.nisms: breakdown o f th. aqueousblood barrier, enzymatic degradation, and liberation of locally syn th.sized proteins.

关键词：蛋白组学蛋白印迹细胞角蛋白排斥反应白内障眼抗胰蛋白酶丝氨酸蛋白酶总蛋白含量质谱测定法载脂蛋白

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