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Belowground Tritrophic Food Chain Modulates Soil Respiration in Grasslands

Pedosphere 2018年第1期28卷 114-123页

作者： Andrey S.ZAITSEV Klaus BIRKHOFER Klemens EKSCHMITT volkmar wolters Institute of Animal Ecology Justus-Liebig-University of Giessen Department of Ecology Brandenburg University of Technology Cottbus-Senftenberg

Edaphic biota significantly affects several essential ecological functions such as C-storage, nutrient turnover, and ***, it is not completely understood how belowground animal contribution to these functions changes in grasslands subject to different land use types. A microcosm experiment was carried out to test the effect of a tritrophic food chain on CO_2 release from grassland soils. Soil was collected from three differently managed grassland systems(meadow, pasture, and mown pasture) located in three distinct German regions that cover a north-south gradient of approximately 500 km. The tritrophic food chain comprised natural edaphic microflora, nematodes, and predatory gamasid mites. The experimental design involved a full factorial combination of the presence and absence of nematodes and gamasid mites. Nematodes significantly increased the CO_2 emissions in most treatments,but the extent of this effect varied with land use type. The fact that grazing by nematodes stimulated the metabolic activity of the edaphic microflora over a wide range of grassland soils highlighted the critical impact of the microfauna on ecosystem services associated with soil organic matter dynamics. Gamasids slightly amplified the effect of nematodes on microbial metabolic activity,but only in the pastures. This effect was most probably due to the control of nematode abundance. The fact that gamasid addition also augmented the impact of environmental conditions on nematode-induced modulation of soil respiration highlighted the need for including land use differences while evaluating soil fauna contribution to soil processes. To conclude, the differential response of the investigated tritrophic food chain to different grassland management systems suggests that adverse effects of land use intensification on important soil processes such as atmospheric C-release could potentially be reduced by using management methods that preserve essential features of the belowground fo

关键词： CO_2 emission land use microcosm nematodes predatory mites soil fauna soil food webs

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How do soil fauna and soil microbiota respond to beech forest growth?

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Current Zoology 2009年第4期55卷 272-278页

作者： Matthieu CHAUVAT Andrei S.ZAITSEV Ernst GABRIEL volkmar wolters Department of Animal Ecology Justus Liebig University

The dynamics and performance of soil biota during forest rotation were studied in monoculture beech stands forming a chronosequence of four different age-classes(30,62,111,153 yr).Biomass was monitored in major groups of microflora,microfauna,mesofauna,and *** availability(litter layer,soil organic mater),biomass of the two dominant decomposer groups(microflora,earthworms)as well as the biomass of mesofauna and microfauna were found to remain quite stable during forest ***,the marked increase of the biomasses of primary decomposers(fungi,saprophagous macroinvertebrates)in the 62-year-old stand,followed by an increase of the biomasses of macropredators in the 111-year-old stand,indicate substantial changes of several components of edaphic communities during forest ***,constant values of soil respiration suggest that the overall performance of the soil food web does not change during beech forest ***,the decomposer system of lowland managed beech forests on calcareous soils seems to be very stable over *** suggest that earthworm activity might have masked impacts of forest development on other soil biota and led to an astounding stability of decomposer assemblages during beech forest rotation.

关键词： Beech Chronosequence Ecosystem performance Soil food-web Forest

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Unmasking metabolic dependencies in pancreatic cancer: aberrant polyamine synthesis as a promising new therapeutic target

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Signal Transduction and Targeted Therapy 2023年第11期8卷 5151-5152页

作者： Gerrit wolters-Eisfeld Thilo Hackert Cenap Güngör Department of General Visceral and Thoracic SurgeryUniversity Medical Center Hamburg-EppendorfHamburgGermany

In a recently published Nature article,Lee et *** distinct metabolic dependencies of pancreatic ductal adenocarcinoma(PDA)cells compared to normal tissue,and discovered that PDA cells exhibited a unique reliance on de novo ornithine synthesis from glutamine,rather than *** aberrant polyamine synthesis offers a promising therapeutic target for pancreatic cancer.

关键词： synthesis den depen

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Teriflunomide provides protective properties after oxygen-glucose-deprivation in hippocampal and cerebellar slice cultures

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Neural Regeneration Research 2021年第11期16卷 2243-2249页

作者： Anna wolters Judith Reuther Philipp Gude Thomas Weber Carsten Theiss Heike Vogelsang Veronika Matschke Institute of Anatomy Department of CytologyMedical FacultyRuhr University BochumBochumGermany Department of Anaesthesiology and Intensive Care Medicine Ruhr University BochumSt.Josef HospitalBochumGermany

One of the major challenges in emergency medicine is out-of-hospital cardiac arrest(OHCA).Every year,about 53–62/100000 people worldwide suffer an out-of-hospital cardiac arrest with serious consequences,whereas persistent brain injury is a major cause of morbidity and mortality of those surviving a cardiac ***,only few and insufficient strategies are known to limit neurological damage of ischemia and reperfusion *** aim of the present study was to investigate whether teriflunomide,an approved drug for treatment of relapsing-remitting-multiple-sclerosis,exerts a protective effect on brain cells in an in vitro model of ***,organotypic slice cultures from rat hippocampus and cerebellum were exposed to oxygen-glucose-deprivation and subsequently treated with *** administration of teriflunomide in the reperfusion time on both hippocampal and cerebellar slice cultures significantly decreased the amount of detectable propidium iodide signal compared with an untreated culture,indicating that more cells survive after ***,hippocampal slice cultures showed a higher vulnerability to ischemic conditions and a more sensitive response to teriflunomide compared with cerebellar slice *** study suggests that teriflunomide,applied as a post-treatment after an oxygenglucose-deprivation,has a protective effect on hippocampal and cerebellar cells in organotypic slice cultures of *** procedures were conducted under established standards of the German federal state of North Rhine Westphalia,in accordance with the European Communities Council Directive 2010/63/EU on the protection of animals used for scientific purposes.

关键词： brain damage cardiac arrest cell death hypoxic chamber ischemia organotypic slice cultures post-treatment resuscitation

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Immunohistochemical CD3 staining detects additional patients with celiac disease

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World Journal of Gastroenterology 2015年第24期21卷 7553-7557页

作者： Amani Mubarak Victorien M wolters Roderick HJ Houwen Fiebo JW ten Kate Department of Pediatric Gastroenterology University Medical Center Utrecht Wilhelmina Children’s Hospital Department of Pathology University Medical Center Utrecht

AIM: To investigate whether performing immuno-histochemical CD3 staining, in order to improve the detection of intra-epithelial lymphocytosis, has an additional value in the histological diagnosis of celiac ***: Biopsies obtained from 159 children were stained by hematoxylin and eosin(HE) and evaluated using the Marsh classification. CD3 staining was subsequently evaluated separately and ***: Differences in evaluation between the routine HE sections and CD3 staining were present in 20(12.6%) cases. In 10(6.3%) patients the diagnosis of celiac disease(Marsh Ⅱ and Ⅲ) changed on examination of CD3 staining: in 9 cases, celiac disease had initially been missed on the HE sections, while 1 patient had been over-diagnosed on the routine sections. In all patients, the final diagnosis based on CD3 staining, was concordant with serological results, which was not found previously. In the other 10(12.3%) patients, the detection of sole intra-epithelial lymphocytosis(Marsh Ⅰ) improved. Nine patients were found to have Marsh Ⅰ on CD3 sections, which had been missed on routine sections. Interestingly, the only patient with negative serology had Giardiasis. Finally, in 1 patient with negative serology, in whom Marsh Ⅰ was suspected on HE sections, this diagnosis was withdrawn after evaluation of the CD3 ***: Staining for CD3 has an additional value in the histological detection of celiac disease lesions, and CD3 staining should be performed when there is a discrepancy between serology and the diagnosis made on HE sections.

关键词： Celiac disease Diagnosis Intra-epitheliallymphocytosis Histology Marsh classification CD3staining

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Cell based therapy in Parkinsonism

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Translational Neurodegeneration 2013年第1期2卷 79-88页

作者： Johannes PJM de Munter Chongsik Lee Erik Ch wolters Department of Neurosciences University Maastricht MaastrichtThe Netherlands Amarna Stem Cells Group MaastrichtThe Netherlands Department of Neurology Asan Medical Center University of UlsanSeoelSouth Korea Department of Neurology UniversitatsSpitalZurichSwitzerland

Parkinson’s disease(PD)is a synucleinopathy-induced chronic progressive neurodegenerative disorder,worldwide affecting about 5 million *** of yet,actual therapies are symptomatic,and neuroprotective strategies are an unmet *** to their capability to transdifferentiate,to immune modulate and to increase neuroplasticity by producing neurotrophic factors,adult stem cells(ASC)might fill this *** research in 6-hydroxydopamine(6-OHDA)and/or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)lesioned animals established persistent improvements of motor behavior after ***/histochemical measurements in these animals evidenced an intracerebral applied ASC-induced increase of Tyrosine Hydroxylase-positive(TH^(+))cells with increased striatal dopamine levels,suggesting cell ***,clinical experience with subventricular applied ASCs in PD patients,although limited,is encouraging,evidencing neurorescue especially during the early phase of the *** multiple system atrophy(MSA)or progressive supranuclear palsy(PSP)patients,though,only marginal reduced progression of natural progression could be established after subventricular or intravasal ASC implantations.

关键词： Adult stem cells Parkinson’s disease Multiple system atrophy BDNF GDNF Expanded MSC Preclinical

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Tissue transglutaminase levels above 100 U/mL and celiac disease:A prospective study

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World Journal of Gastroenterology 2012年第32期18卷 4399-4403页

作者： Amani Mubarak Victorien M wolters Frits HJ Gmelig-Meyling Fiebo JW ten Kate Roderick HJ Houwen Department of Pediatric Gastroenterology Wilhelmina Children's HospitalUniversity Medical Center Utrecht3508 AB UtrechtThe Netherlands Department of Immunology University Medical Center Utrecht3508 AB UtrechtThe Netherlands Department of Pathology University Medical Center Utrecht3508 AB UtrechtThe Netherlands

AIM:To investigate whether a tissue-transglutaminase antibody(tTGA) level ≥ 100 U/mL is sufficient for the diagnosis of celiac disease(CD).METHODS:Children suspected of having CD were prospectively included in our study between March 2009 and September *** patients with immune globulin A deficiency and all patients on a gluten-free diet were excluded from the ***-endomysium antibodies(EMA) were detected by means of immunofluorescence using sections of distal monkey esophagus(EUROIMMUN,Luebeck,Germany).Serum anti-tTGA were measured by means of enzyme-linked immunosorbent assay using human recombinant tissue transglutaminase(ELiA Celikey IgA kit Phadia AB,Uppsala,Sweden).The histological slides were graded by a single experienced pathologist using the Marsh classification as modified by *** Ⅱ and Ⅲ lesions were considered to be diagnostic for the *** positive predictive values(PPVs),negative predictive values(NPVs),sensitivity and specificity of EMA and tTGA along with their 95% CI(for the cut off values > 10 and ≥ 100 U/mL) were calculated using histology as the gold standard for ***:A total of 183 children were included in the study.A total of 70(38.3%) were male,while 113(61.7%) were *** age range was between 1.0 and 17.6 years,and the mean age was 6.2 *** hundred twenty(65.6%) patients had a small intestinal biopsy diagnostic for the disease;3 patients had a Marsh Ⅱ lesion,and 117 patients had a Marsh Ⅲ *** the patients without CD,only 4 patients had a MarshⅠ*** the 183 patients,136 patients were positive for EMA,of whom 20 did not have CD,yielding a PPV for EMA of 85%(95% CI:78%-90%) and a corresponding specificity of 68%(95% CI:55%-79%).The NPV and specificity for EMA were 91%(95% CI:79%-97%) and 97%(95% CI:91%-99%),*** levels of tTGA were found in 130 patients,although only 116 patients truly had histological evidence of the *** PPV for tTGA was 89%(95% CI:82%-94%),and th

关键词： Celiac disease Diagnosis Serology Antitissue-transglutaminase antibodies Anti-endomysium antibodies

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最终元件确保一个完整的安全回路——莫克威尔德阀门有限责任公司高度一体化（压力）保护系统产品部经理Rens wolters详述隔离阀在重要的安全应用中的功能和要求

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经济导报（石油天然气） 2008年第2期 50-53页

作者： Rens wolters 莫克威尔德阀门有限责任公司高度一体化（压力）保护系统产品部经理

安全切断阀的一个众所周知的术语是紧急切断阀，或者简称作ESD阀。ESD阀的功能是根据停车系统的要求关闭阀门，因此执行的是一个关键的安全功能。紧急停车系统可以追溯到阀门安全系统的时代，阀门安全系统由第一层保护（紧急停车系统本... 详细信息

安全切断阀的一个众所周知的术语是紧急切断阀，或者简称作ESD阀。ESD阀的功能是根据停车系统的要求关闭阀门，因此执行的是一个关键的安全功能。紧急停车系统可以追溯到阀门安全系统的时代，阀门安全系统由第一层保护（紧急停车系统本身）和第二层保护组成，第二层保护是指安全泄放系统（安全泄放阀）。如果过程控制系统失灵，ESD系统将试图停车。如果停车失败，泄放阀触发，把压力、或者温度降低到一个可以接受的水平。

关键词：保护系统安全功能阀门压力一体化经理产品

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Children with celiac disease and high tTGA are genetically and phenotypically different

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World Journal of Gastroenterology 2013年第41期19卷 7114-7120页

作者： Amani Mubarak Eric Spierings Victorien M wolters Henny G Otten Fiebo JW ten Kate Roderick HJ Houwen Department of Pediatric Gastroenterology University Medical Center UtrechtWilhelmina Children’s Hospital3508 AB UtrechtThe Netherlands Department of Immunology University Medical Center Utrecht3508 GA UtrechtThe Netherlands Department of Pathology University Medical Center Utrecht3508 GA UtrechtThe Netherlands

AIM:To investigate whether celiac disease(CD)patients with tissue-transglutaminase antibody(tTGA)≥100 U/mL are different from patients with lower tTGA ***:Biopsy-proven(MarshⅢ)pediatric CD patients(n=116)were prospectively included between March 2009 and October *** biopsies were evaluated by a single pathologist who was blinded to all of the patients’clinical *** patients were distributed into 2 groups according to their tTGA level,which was measured using enzyme-linked immunoassay:tTGA≥100 U/mL and Ttga<100 U/*** patients’characteristics,symptoms,human leukocyte antigen(HLA)genotype and degree of histological involvement were compared between the 2 ***:A total of 34(29.3%)children had tTGA values<100 U/mL and 82(70.7%)tTGA levels of≥100 U/*** with high tTGA levels had lower average body weight-for-height standard deviation scores(SDS)than did patients with tTGA<100 U/mL(-0.20±1.19 SDS vs 0.23±1.03 SDS,P=0.025).In the low tTGA group,gastrointestinal symptoms were more common(97.1%vs 75.6%,P=0.006).More specifically,abdominal pain(76.5%vs 51.2%;P=0.012)and nausea(17.6%vs 3.7%,P=0.018)were more frequent among patients with low *** contrast,patients with solely extraintestinal manifestations were only present in the high tTGA group(18.3%,P=0.005).These patients more commonly presented with aphthous stomatitis(15.9%vs 0.0%,P=0.010)and anemia(32.9%vs 11.8%,P=0.019).In addition,when evaluating the number of CD-associated HLA-DQ heterodimers(HLA-DQ2.5,HLA-DQ2.2 and HLA-DQ8),patients with low tTGA levels more commonly had only1 disease-associated heterodimer(61.8%vs 31.7%,P=0.005),while patients with high tTGA more commonly had multiple ***,patients with tTGA≥100 U/mL more often had a MarshⅢc lesion(73.2%vs 20.6%,P≤0.001)while in patients with low tTGA patchy lesions were more common(42.4%vs6.8%,P≤0.001).CONCLUSION:Patients with tTGA≥100 U/mL show several signs of more advanced *** also carry a larger

关键词： Celiac disease Serology Anti-tissue transglutaminase antibodies Human leukocyte antigen Phenotype

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Emission from quantum-dot high-β microcavities: transition from spontaneous emission to lasing and the effects of superradiant emitter coupling

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Light(Science & Applications) 2017年第1期6卷 638-645页

作者： Sören Kreinberg Weng W Chow Janik wolters Christian Schneider Christopher Gies Frank Jahnke Sven Höfling Martin Kamp Stephan Reitzenstein 无 Institut für Festkörperphysik Technische Universität BerlinBerlin 10623Germany Sandia National Laboratories AlbuquerqueNM 87185-1086USA Lehrstuhl für Technische Physik Universität WürzburgWürzburg 97074Germany School of Physics and Astronomy University of St AndrewsSt Andrews KY169SSUK Institute for Theoretical Physics University of BremenBremen 28334Germany

Measured and calculated results are presented for the emission properties of a new class of emitters operating in the cavity quantum electrodynamics *** structures are based on high-finesse GaAs/AlAs micropillar cavities,each with an active medium consisting of a layer of InGaAs quantum dots(QDs)and the distinguishing feature of having a substantial fraction of spontaneous emission channeled into one cavity mode(highβ-factor).This paper demonstrates that the usual criterion for lasing with a conventional(lowβ-factor)cavity,that is,a sharp non-linearity in the input–output curve accompanied by noticeable linewidth narrowing,has to be reinforced by the equal-time second-order photon autocorrelation function to confirm *** paper also shows that the equal-time second-order photon autocorrelation function is useful for recognizing superradiance,a manifestation of the correlations possible in high-βmicrocavities operating with *** terms of consolidating the collected data and identifying the physics underlying laser action,both theory and experiment suggest a sole dependence on intracavity photon *** for this assertion comes from all our measured and calculated data on emission coherence and fluctuation,for devices ranging from light-emitting diodes(LEDs)and cavity-enhanced LEDs to lasers,lying on the same two curves:one for linewidth narrowing versus intracavity photon number and the other for g(2)(0)versus intracavity photon number.

关键词： coherence laser physics microlasers nanolasers optoelectronics photon statistics quantum dots quantum optics

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