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Site-Specific Conjugation of Cell Wall Polyrhamnose to Protein SpyAD Envisioning a Safe Universal Group A Streptococcal Vaccine

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Infectious Microbes & Diseases 2021年第2期3卷 87-100页

作者： Nina j.Gao Satoshi Uchiyama Lucy Pill Samira Dahesh joshua Olson Leslie Bautista Shilpa Maroju Aym Berges janet ZLiu Raymond HZurich Nina Mvan sorge jeff Fairman Neeraj Kapoor Victor Nizet Division of Host-Microbe Systems and Therapeutics Department of PediatricsUC San DiegoLa JollaCaliforniaUSA Vaxcyte Inc.Foster CityCaliforniaUSA Department of Medical Microbiology and Infection Prevention Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Netherlands Reference Laboratory for Bacterial Meningitis Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Skaggs School of Pharmacy and Pharmaceutical Sciences UC San DiegoLa JollaCaliforniaUSA

Development of an effective vaccine against the leading human bacterial pathogen group A Streptococcus(GAS)is a public health *** species defining group A cell wall carbohydrate(GAC,Lancefield antigen)can be engineered to remove its immunodominant N-acetylglucosamine(GlcNAc)side chain,implicated in provoking autoimmune cross-reactivity in rheumatic heart disease,leaving its polyrhamnose core(GACPR).Here we generate a novel protein conjugate of the GACPR and test the utility of this conjugate antigen in active *** of conjugation to a standard carrier protein,we selected SpyAD,a highly conserved GAS surface protein containing both B-cell and T-cell epitopes relevant to the bacterium that itself shows promise as a vaccine *** was synthesized using the XpressTM cell-free protein expression system,incorporating a non-natural amino acid to which GACpr was conjugated by site-specific click chemistry to yield high molecular mass SpyAD-GACPR conjugates and avoid disruption of important T-cell and B-cell immunological *** conjugated SpyAD-GACPR elicited antibodies that bound the surface of multiple GAS strains of diverse M types and promoted opsonophagocytic killing by human *** immunization of mice with a multivalent vaccine consisting of SpyAD-GACPR,together with candidate vaccine antigens streptolysin O and C5a peptidase,protected against GAS challenge in a systemic infection model and localized skin infection model,without evidence of cross reactivity to human heart or brain tissue *** general approach may allow GAC to be safely and effectively included in future GAS subunit vaccine formulations with the goal of broad protection without autoreactivity.

关键词： group A Streptococcus Streptococcus pyogenes group A carbohydrate Lancefield antigen conjugate vaccine XpressCFTM non-natural amino acids SpyAD Introduction

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Soil Organic Matter and Grain Yield of Rainfed Maize in Luvisols of Campeche, México

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Agricultural Sciences 2019年第12期10卷 1602-1613页

作者： juan Medina-Méndez Víctor H. volke.Haller josé I. Cortés-Flores Arturo Galvis-Spínola María de j. Santiago-Cruz Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Campo Experimental Edzná Campeche México Colegio de Postgraduados Texcoco México

The organic matter is an important soil component, due its favorable effects on soil physical and chemical properties and, by consequence, on crop yields. So, the objective of this work was quantify the changes through the time of soil organic matter in rainfed maize (Zea mays L.) system, in a period of 30 years of continuous crop and in relation to system of natural vegetation system, and also the effects of these changes on the crop yield, in Luvisols soils of State of Campeche, México. In production cycle of 2004, 53 farmer plots in rainfed maize system of different land use time and five sites of forest natural vegetation system were sampled for soil, to quantify physical and chemical properties of soil, and it was taken data about climate, system management and crop yield. The data were analiced by regression analysis, considered the organic matter as function of land use time and factors of soil and management of system, and the crop yield as function of soil organic matter and factors of soil, climate and management of system. The soil organic matter diminished from 5.68% in forest natural vegetation system to 3.59% after 16 - 30 years of cultivation, and the soil annual incorporation of vegetative mulch (weeds and stubble of maize) increased the organic matter from 0.14% after 1 - 5 years to 0.46% after 16 - 30 years of cultivation. Without fertilization, the diminution of soil organic matter caused 907 kg⋅ha−1 less of maize yield, and the fertilization with 200 kg⋅ha−1 of diamonic phosphate (36 kg⋅N⋅ha−1 and 92 kg P2O5 ha−1) increased the maize yield 1224 kg⋅ha−1 after 1 - 5 years and 1421 kg⋅ha−1 after 16 - 30 years of cultivation, but not compensed less maize yield of 711 kg⋅ha−1 due the diminution of soil organic matter.

关键词： Zea mays L. Annual Crops Forest Natural Vegetation Land Use Time Soil Properties

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国际金融管理纲要版

国际金融管理纲要

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1985年

作者：（美）韦斯顿（j.F.Weston）（美）佐尔格（B.W.sorge）李靖国译

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阿片类镇痛剂对门诊肿瘤病人的治疗

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德国医学 1999年第1期16卷 29-32页

作者： j.sorge.B.L ders C.Werry I.Pichlmayr 苗青朱晓悦 Zentrum Anasthesiologie der Medizinischen Hochschule Abteilung Ⅳ-Krankenhaus OststadtPodbielskistr.380D-30659 HannoverGermany 陕西省人民医院陕西省人民政府新闻办公室

疼痛是肿瘤疾病的常见伴随症状。在多数病人中,这种疼痛能按照一定的准则和原理(所谓的WHO-Stufenschemas)施行药物疗法很好控制。有创技术的使用仅在极少情况下适用。利用有创技术对肿瘤病人的全面治疗,不论是在医院或是诊所进行都是... 详细信息

疼痛是肿瘤疾病的常见伴随症状。在多数病人中,这种疼痛能按照一定的准则和原理(所谓的WHO-Stufenschemas)施行药物疗法很好控制。有创技术的使用仅在极少情况下适用。利用有创技术对肿瘤病人的全面治疗,不论是在医院或是诊所进行都是可能的。

关键词：肿瘤疼痛药物疗法阿片类镇痛剂

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