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Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

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World Journal of Stem Cells 2021年第5期13卷 452-469页

作者： vladimir P Baklaushev Oleg V Durov vladimir A Kalsin Eugene V Gulaev Sergey V Kim Ilya L Gubskiy Veronika A Revkova Ekaterina M Samoilova Pavel A Melnikov Dzhina D Karal-Ogly Sergey V Orlov Alexander V Troitskiy vladimir P chekhonin Alexander V Averyanov Jan-Eric Ahlfors Biomedical Research Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Neurosurgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBAMoscow 115682MoskvaRussia Department of Anesthesiology N.N.Blokhin Russian Cancer Research CentreMoscow 115478MoskvaRussia Radiology and Clinical Physiology Scientific Research Center Federal center of brain research and neurotechnologies of the Federal Medical Biological AgencyMoscow 117997Russia Department of Neurobiology Serbsky National Medical Research Center for Psychiatry and NarcologyMoscow 119992MoskvaRussia Department of Primatology Russian Acad Med SciResearch Institute of Medical PrimatologySochi 119992SochiRussia Department of Vascular Surgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Basic and Applied Neurobiology V.P.Serbsky Federal Medical Research Center for Psychiatry and NarcologyRussia Department of Medical Nanobiotechnology Pirogov Russian National Research Medical University(RNRMU)Moscow 115682MoskvaRussia New World Laboratories Laval H7V 4A7QuebecCanada

BACKGROUND The development of regenerative therapy for human spinal cord injury(SCI)is dramatically restricted by two main challenges:the need for a safe source of functionally active and reproducible neural stem cells and the need of adequate animal models for preclinical *** reprogramming of somatic cells into neuronal and glial precursors might be a promising solution to the first *** use of non-human primates for preclinical studies exploring new treatment paradigms in SCI results in data with more translational relevance to human *** To investigate the safety and efficacy of intraspinal transplantation of directly reprogrammed neural precursor cells(drNPCs).METHODS Seven non-human primates with verified complete thoracic SCI were divided into two groups:drNPC group(n=4)was subjected to intraspinal transplantation of 5 million drNPCs rostral and caudal to the lesion site 2 wk post injury,and lesion control(n=3)was injected identically with the equivalent volume of *** Follow-up for 12 wk revealed that animals in the drNPC group demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of somatosensory evoked potential and motor evoked potential of injured *** resonance diffusion tensor imaging data confirmed the intraspinal transplantation of drNPCs did not adversely affect the morphology of the central nervous system or cerebrospinal fluid *** immunohistochemical analysis showed that drNPCs maintained SOX2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk,migrating to areas of axon growth *** Our data demonstrated that drNPC transplantation was safe and contributed to improvement of spinal cord function after acute SCI,based on neurological status assessment and neurophysiological recovery within 12 wk after *** functional improvement described was not associated with neuronal differentiation of th

关键词： Direct cell reprogramming Neural precursor cells Directly reprogrammed neural precursor cells Spinal cord injury Nonhuman primates Regenerative therapy,Evoked potentials

Numerical Simulation of Multicomponent Alloy Solidification

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Journal of Mechanics Engineering and Automation 2014年第10期4卷 828-837页

作者： vladimir pavlovich Ginkin Svetlana Mikhailovna Ganina Andrey Valentinovich Kartavykh MA TEK (Mathematical Ecology) Ltd. Obninsk 249033 Russia Department of Mathematics and Software Institute for Physics and Power Engineering Obninsk 249033 Russia Department of Physical Chemistry National University of Science and Technology "MISIS" Moscow 119049 Russia Department of Functional and Constructional Nanomaterials Technological Institute for Superhard and Novel Carbon MaterialsMoscow 142190 Russia

A non-equilibrium model of multicomponent melt solidification has been developed in which a Stefan problem with two boundaries is solved numerically, the boundaries being between the solid phase and the two-phase transition zone and between the two-phase transition zone and the liquid phase. The two-phase zone is represented as a porous medium with variable porosity. The additional force resisting the melt flow due to porosity and introduced by analogy with Darcy＇s law is taken into account. Computer simulation has been performed of the experiment on Sn-20 wt.%Pb binary alloy solidification by the method of downward-directed crystallization along the gravity vector. The paper shows the results of a quasi two-dimensional benchmark experiment on horizontal （i.e., at the right angle to the gravity vector） directional solidification of a binary Sn-3 wt.%Pb alloy. The calculations were done using two crystallization models： the equilibrium model and the non-equilibrium one. It is shown that the non-equilibrium model gives a better description of the thermal field evolution and solute distribution caused by natural convection.

关键词： Solidification binary alloy mushy zone macrosegregation convection heat-mass transfer modelling comparison withexperimental results.

Sports Game Radial Basketball in Physical Education of Preschool Children

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Journal of Sports Science 2016年第6期4卷 374-376页

作者： Ovchinnikov vladimir pavlovich Nesmeyanov Anatoly Alexandrovich Vasilevskiy Dmitriy Konstantinovich Russian State Pedagogical University A. 1. Herzen Saint-Petersburg 190000 Russia President of the Federation of Piterbasket Saint-Petersburg 190000 Russia Leningrad State University Alexander Pushkin St. Petersburg 190000 Russia

The article is devoted to the application of new radial sports games basketball in physical education of preschool children. The benefits of a new game： expanding the playing space attributable to each player, limiting the opposition defenders and facilitates the use of gaming devices. The use of innovative methods in physical training of preschool children gives you the opportunity to solve successfully the whole complex of educational issues and the full development of the child.

关键词： Radial sports game basketball preschool children physical education innovation of process of physical education.

Geological Structure and Historical Development of Pre-Jurrasic Basement of West Siberia Oil- and Gas-Bearing Megabasin Karabash Zone

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Open Journal of Geology 2022年第10期12卷 717-738页

作者： Kirill Svjatoslavich Ivanov Nikolay pavlovich Kostrov Yuriy Viktorovich Erokhin vladimir Sergeevich Ponomarev Institute of Geology and Geochemistry Urals Branch of Russian Academy of Sciences Yekaterinburg Russia

An additional comprehensive study of the west Siberia Karabash zone basement has been conducted. Core samples from more than 300 wells that opened the rocks of the basement were analyzed by different methods. A new map of the pre-Jurassic basement of Karabash zone of the west part of the Khanty-Mansi Autonomous Okrug (KhMAO) has been made. For the first time for the West Siberia megabasin U-Pb dating was made for samples from Shaim-Kuznetsovsk meganticlinorium and late to middle Devonian ages were obtained. The Devonian ages was obtained for metamorphic complexes of the region and, in general, for the basement of West Siberia. The complexes considered as Precambrian before that. The obtained results enable significantly to clarify more exact history of pre-Jurassic rock forming for the basement of the west part of West Siberia craton and its structure in the limits of Karabash zone. Thus, obtained data testify that in pre-Jurassic basement of the west part of the West Siberia (Karabash zone), filling grabens basalt effusion began during Permian time (probably at the end of early Permian) at sublatitudinal compression, that is, at collision, possibly immediately after or subsynchronously with the origin of the granite. On the edge of Permian and Triassic (or in early Triassic), sublatitudinal compression changed into stretching, and submeridional grabens came to existence and basalt effusion came up to the maximum.

关键词： Zircon Age Definition West Siberia Pre-Jurassic Basement Triassic Graben-Rifts

Some Aspects Affecting the Molecular Mechanisms of Eukaryotic Adaptation under Global Warming

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Journal of Pharmacy and Pharmacology 2019年第11期7卷 578-583页

作者： Michael Ivanovich Gill vladimir pavlovich Novikov Lyubov Igorivna Denisyuk Igor Yurievich Gorbatenko Faculty of Technology of Production and Processing of Livestock Products Standardization and Biotechnology Mykolaiv National Agrarian University Mykolaiv 54010 Ukraine Department of Technology of Bioactive Compounds Pharmacy and Biotechnology Lviv Polytechnic National University Lviv 79000 Ukraine Kiev Clinical Ophthalmologic Hospital Kiev 01135 Ukraine Department of Animal Genetics and Biotechnology Mykolaiv National Agrarian University Mykolaiv 54010 Ukraine

Global warming is an irreversible process resulting in the deterioration of living conditions for various organisms, including the most important agricultural species. So-called ζ^32 factor of Escherichia coli is embedded into the RNA-thermosensor in the λ cIII gene and plays an important role in the regulation of bacterial response to hightened temperatures. Expression of heat/cold-shock genes and some virulence genes in response to temperature changes is coordinated by the genome. There are some known RNA-thermosensors with different structures that provide a functional control of the diversity of cellular processes. The most common RNA-thermosensor is the ROSE-element suppressing expression of heat-shock genes. A common feature is functionally important and it is elimination that makes the RNA-thermosensor insensitive to high temperatures. In this paper we describe molecular sequences (RNA-thermosensor) whose chemical compounds influence on the homeostatic temperature regulation, namely, on the corresponding enzymes. Though the data on RNA-thermosensors we obtained for microorganisms it is maybe possible in the long run to change the animal genome at the molecular level by the insertion of these sequences or cultivation of symbiotic microorganisms, which may be used for production of biologically active compounds. In addition, such insertions would probably be able to reduce the negative effect of high environmental temperatures on living organisms.

关键词： Global warming RNA-thermosensors DNA ribonucleic acid (RNA) enzymes

A System for Road Pavement Composite Material Deformation

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Engineering（科研） 2012年第12期4卷 958-965页

作者： Viktor pavlovich Basuev vladimir Nikolaevich Venik Yuriy Konstantinovich Rybin Alexander Karlovich Efa National Research Tomsk Polytechnic University Tomsk Russia Road Construction Company “AVTOBAN” Moscow Russia Tomsky State University of Architecture and Building Tomsk Russia

For a comprehensive experimental evaluation of the material quality, forecast of the properties and parameter change of the bituminous material was made at the time under the impact of external factors, they are subjected to the necessary tests. In the article the automated set “Tomsk-Asphalt-Test” for determining the elastic modulus of the specimens made of bituminous materials was used in road pavements, maximally close to natural conditions of operation of highways of the Siberian region inRussiaare described. The automated set contains: electromechanical, climate, electronic, PC and software subsystem. The operation principle is a short-time deformation of the asphalt specimens;measurement of physical values: the stress, strain, variation of the size line and temperature of the asphalt pavement material test specimen, converting the measured values into electrical signals, their program processing and visualization. The control of testing and viewing results of measurements is carried out in accordance with the menu software subsystem. The results of calculations: the maximum values of vertical load the difference between the maximum horizontal deformation value and the value measured last after specimen of asphalt material loading for each test cycle, the sum of the differences of the horizontal deformation values of the two sensors and modulus of elasticity.

关键词： Road Pavement Composite Asphalt Compression Specimen Deformation Displacement Modulus of Elasticity

Liquid Metal Coolants Technology for Fast Reactors

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材料科学与工程（中英文B版） 2011年第7期1卷 913-928页

作者： Poplavsky vladimir Mikhailovich Efanov Alexander Dmitrievich Kozlov Fedor Alekseevich Orlov Yury Ivanovich Sorokin Alexander pavlovich FSUE State Scientific Center of the Russian Federation-Institute for Physics and Power Engineering named after A.I. Leypunsky Obninsk Russia

On the Fractal Mechanism of Interrelation Between the Genesis, Size and Composition of Atmospheric Particulate Matters in Different Regions of the Earth

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Atmospheric and Climate Sciences 2011年第3期1卷 120-133页

作者： Vitaliy D Rusov Radomir Ilic Radojko Jacimovic vladimir N pavlovich Yuriy A Bondarchuk vladimir N. Vaschenko Tatiana N Zelentsova Margarita E Beglaryan Elena P Linnik vladimir P Smolyar Sergey I Kosenko Alla A Gudyma Odessa National Polytechnic University Shevchenko av. 1 65044 Odessa Ukraine

Experimental data from the National Air Surveillance Network of Japan from 1974 to 1996 and from inde-pendent measurements performed simultaneously in the regions of Ljubljana (Slovenia), Odessa (Ukraine) and the Ukrainian “Academician Vernadsky” Antarctic station (64o15W;65o15S), where the air elemental composition was determined by the standard method of atmospheric particulate matter (PM) collection on nucleopore filters and subsequent neutron activation analysis, were analyzed. Comparative analysis of dif-ferent pairs of atmospheric PM element concentration data sets, measured in different regions of the Earth, revealed a stable linear (on a logarithmic scale) correlation, showing a power law increase of every atmos-pheric PM element mass and simultaneously the cause of this increase – fractal nature of atmospheric PM genesis. Within the framework of multifractal geometry we show that the mass (volume) of atmospheric PM elemental components has a log normal distribution, which on a logarithmic scale with respect to the random variable (elemental component mass) is identical to normal distribution. This means that the parameters of two-dimensional normal distribution with respect to corresponding atmospheric PM-multifractal elemental components measured in different regions, are a priory connected by equations of direct and inverse linear regression, and the experimental manifestation of this fact is the linear correlation between the concentra-tions of the same elemental components in different sets of experimental atmospheric PM data.

关键词： Atmospheric aerosols Multifractal Neutron activation analysis South Pole Ukrainian Antarctic station

应用磁共振扩散张量成像技术诊断大鼠创伤后脊髓空洞症

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中华实验外科杂志 2018年第7期35卷 1303-1303页

作者：陈凯张超李之珺付嘉园圆 vladimir P. chekhonin 天津市第四中心医院肿瘤介入科 300140 天津医科大学肿瘤医院骨与软组织肿瘤科 300060 天津医科大学肿瘤医院放射科 300060 天津医科大学临床医学院 300270 Moscow Pirogov Russian National Research Medical University117997

创伤后脊髓空洞症（PTS）是脊髓损伤（SCI）后髓内囊性退变。PTS可加剧SCI后症状。传统磁共振扫描（MRI）难以对PTS进行早期诊断。磁共振弥散张量成像（DTI）已应用于SCI研究。我们基于7．0TMRI通过DTI分析大鼠SCI模型，评估DTI早期诊断... 详细信息

创伤后脊髓空洞症（PTS）是脊髓损伤（SCI）后髓内囊性退变。PTS可加剧SCI后症状。传统磁共振扫描（MRI）难以对PTS进行早期诊断。磁共振弥散张量成像（DTI）已应用于SCI研究。我们基于7．0TMRI通过DTI分析大鼠SCI模型，评估DTI早期诊断PTS可行性。

关键词：磁共振扩散张量成像脊髓空洞症创伤后技术诊断大鼠应用磁共振弥散张量成像 SCI模型