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Phenotyping Key Fruit Quality Traits in Olive Using RGB Images and Back Propagation Neural Networks

Plant Phenomics 2023年第3期5卷 484-500页

作者： Giuseppe Montanaro Angelo Petrozza Laura Rustioni Francesco Cellini vitale nuzzo Università degli Studi della Basilicata 85100 PotenzaItaly ALSIA Agenzia Lucana Sviluppo Innovazione in AgricolturaMetapontum Agrobios Research Center75010 MetapontoItaly Department of Biological and Environmental Sciences and Technologies University of SalentoLecceItaly

To predict oil and phenol concentrations in olive fruit,the combination of back propagation neural networks(BPNNs)and contact-less plant phenotyping techniques was employed to retrieve RGB image-based digital proxies of oil and phenol *** of cultivars(×3)differing in ripening time were sampled(~10-day interval,×2 years),pictured and analyzed for phenol and oil *** to this,fruit samples were pictured and images were segmented to extract the red(R),green(G),and blue(B)mean pixel values that were rearranged in 35 RGB-based colorimetric *** BPNNs were designed using as input variables(a)the original 35 RGB indexes,(b)the scores of principal components after a principal component analysis(PCA)pre-processing of those indexes,and(c)a reduced number(28)of the RGB indexes achieved after a sparse *** results show that the predictions reached the highest mean R^(2) values ranging from 0.87 to 0.95(oil)and from 0.81 to 0.90(phenols)across the *** addition to the R^(2),other performance metrics were calculated(root mean squared error and mean absolute error)and combined into a general performance indicator(GPI).The resulting rank of the GPI suggests that a BPNN with a specific topology might be designed for cultivars grouped according to their ripening *** present study documented that an RGB-based image phenotyping can effectively predict key quality traits in olive fruit supporting the developing olive sector within a digital agriculture domain.

关键词： Image image supporting

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Use of priming strategies to advance the clinical application of mesenchymal stromal/stem cell-based therapy

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World Journal of Stem Cells 2024年第1期16卷 7-18页

作者： vitale Miceli Department of Research Istituto Mediterraneo per i Trapianti e Terapie ad alta SpecializzazionePalermo 90127Italy

Mesenchymal stromal/stem cells(MSCs)have garnered significant attention in the field of regenerative medicine due to their remarkable therapeutic *** play a pivotal role in maintaining tissue homeostasis and possess diverse functions in tissue repair and recovery in various *** cells are charac-terized by easy accessibility,few ethical concerns,and adaptability to in vitro cultures,making them a valuable resource for cell therapy in several clinical *** the years,it has been shown that the true therapeutic power of MSCs lies not in cell engraftment and replacement but in their ability to produce critical paracrine factors,including cytokines,growth factors,and exosomes(EXOs),which modulate the tissue microenvironment and facilitate repair and regeneration ***,MSC-derived products,such as condi-tioned media and EXOs,are now being extensively evaluated for their potential medical applications,offering advantages over the long-term use of whole ***,the efficacy of MSC-based treatments varies in clinical trials due to both intrinsic differences resulting from the choice of diverse cell sources and non-standardized production *** address these concerns and to enhance MSC therapeutic potential,researchers have explored many priming strategies,including exposure to inflammatory molecules,hypoxic conditions,and three-dimensional culture *** approaches have optimized MSC secretion of functional factors,empowering them with enhanced immunomodulatory,angiogenic,and regenerative properties tailored to specific medical *** fact,various priming strategies show promise in the treatment of numerous diseases,from immune-related disorders to acute injuries and ***,in order to exploit the full therapeutic potential of MSC therapy,the most important challenge is to optimize the modulation of MSCs to obtain adapted cell therapy for specific clinical *** other words,to unlock t

关键词： Mesenchymal stromal/stem cells Therapeutic properties Paracrine effects Cell priming Cell-free therapies Regenerative medicine

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Use of DREAM to assess relative risks of presence of pharmaceuticals and personal care products from a wastewater treatment plant

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Frontiers of Environmental Science & Engineering 2024年第9期18卷 91-104页

作者： Daniela M.Pampanin Daniel Schlenk Matteo vitale Pierre Liboureau Magne O Sydnes Department of Chemistry Bioscience and Environmental EngineeringUniversity of StavangerStavanger 224021Norway Department of Environmental Sciences University of California RiversideRiversideCA 92521USA

Concerns related to environmental risks associated with pharmaceuticals and personal care products(PPCPs)have led researchers to seek methods for assessing and monitoring these contaminants in the aquatic *** and validating risk assessment tools that can evaluate ecological concerns and risks associated with PPCPs is ***,the suitability of a dose-related risk and effect assessment model,which estimates predicted environmental concentrations and allowed comparisons with predicted no effect concentrations determined,in combination with in vitro analyses of the whole effluent toxicity,was verified for the characterization of a PPCP *** of the most utilized PPCPs in Norway were measured in influent and effluent samples and used to parameterize the fate *** than 90%removal was attained for 12 out of 22 detected *** was not dependent on the class or the concentration of the specific substance and varied between 12%and 100%.The PPCPs detected in the discharged wastewater were utilized to assess individual contributions to the risk of the effluent,and no risk was identified for the targeted 30 *** simulations provided valuable information regarding the discharge plume distribution over time,which can aid planning of future environmental monitoring ***(using fish liver cells,PLHC-1)were used for assessing overall effluent toxicity,through cell viability,production of reactive oxygen species,and ethoxyresorufin-O-deethylase(EROD)*** present study may allow regulators to use risk-based strategies over removal criteria for monitoring studies and confirms the importance to take PPCP contamination into consideration when establishing environmental regulations.

关键词： Pharmaceuticals Wastewater effluent Ciprofloxacin Metoprolol Amitriptyline Carbamazepine Modelling Risk assessment Monitoring

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Laparoscopic central pancreatectomy with gastro-pancreatic anastomosis for symptomatic serous cystadenoma:A case report and literature review

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Laparoscopic, Endoscopic and Robotic Surgery 2024年第2期7卷 87-91页

作者： Giuseppe Frazzetta Antonino Picciurro Angela Maffongelli Irene vitale Francesco vitale Daniela Scimeca Michele Amata Anna Calì Ambra Bonaccorso Barbara Scrivo Vincenzo Di Martino Elisabetta Conte Filippo Mocciaro Roberto Di Mitri Pierenrico Marchesa Oncologic Surgery Unit ARNAS Civico HospitalPalermo 90100Italy Gastroenterology Unit ARNAS Civico HospitalPalermo 90100Italy

Surgery for lesions of the proximal part of the pancreatic body or neck can be challenging,and when enucleation is not possible,central pancreatectomy is an *** central pancreatic resection is rarely described worldwide;it is considered a difficult procedure mainly because of the risk of double pancreatic fistula developing at two sites of ***,it seems to be an excellent alternative to distal pancreatectomy or pancreaticoduodenectomy,with the advantages of preserving functioning parenchyma and reducing endocrine and exocrine ***,patients with pancreatic lesions requiring central resection are often managed with the open approach in many hospitals due to the complexity of total laparoscopic central pancreatectomy,which requires advanced laparoscopic skills,expertise and ***,we report a case of a 29-year-old female who underwent total laparoscopic central pancreatic resection with gastro-pancreatic anastomosis for symptomatic serous *** discuss the details of case management and review the relevant literature.

关键词： Laparoscopic Pancreatic surgery Central pancreatectomy Cystadenoma

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Long-term assessment of collagenase treatment for Dupuytren’s contracture:A 10-year follow-up study

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World Journal of Orthopedics 2024年第4期15卷 355-362页

作者： Marco Passiatore vitale Cilli Adriano Cannella Ludovico Caruso Giulia Maria Sassara Giuseppe Taccardo Rocco De Vitis Department of Bone and Joint Surgery ASST-Spedali CiviliBrescia 25123Italy Department of Chirurgie de la Main Centre Hospitalier Interregional Edith CavellBruxelles 1160Belgium Department of Orthopedics Fondazione Policlinico Universitario Agostino Gemelli IRCSSRome 00168Italy

BACKGROUND Enzymatic fasciotomy with collagenase clostridium histolyticum(CCH)has revolutionized the treatment for Dupuytren’s contracture(DC).Despite its benefits,the long-term outcomes remain *** study presented a comprehensive 10-year follow-up assessment of the enduring effects of CCH on patients with *** To compare the short-term(12 wk)and long-term(10 years)outcomes on CCH treatment in patients with *** A cohort of 45 patients was treated with CCH at the metacarpophalangeal(MCP)joint and the proximal interphalangeal(PIP)joint and underwent systematic *** study adhered to multicenter trial protocols,and assessments were conducted at 12 wk,7 years,and 10 years *** Thirty-seven patients completed the 10-year *** 10 years,patients treated at the PIP joint exhibited a 100%***,patients treated at the MCP joint only showed a 50%*** satisfaction varied,with a lower satisfaction reported in PIP joint *** exceeding 20 degrees on the total passive extension deficit was observed,indicating a challenge for sustained *** differences were noted between outcomes at the 7-year and 10-year *** CCH demonstrated sustained efficacy when applied to the MCP ***,caution is warranted for CCH treatment at the PIP joint due to a high level of recurrence and low patient ***-intervention is needed within a decade of treatment.

关键词： Collagenase Xiapex Dupuytren disease Dupuytren recurrence Long term follow-up

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Biofabrication of nanovesicles for brain diseases

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Neural Regeneration Research 2023年第3期18卷 525-526页

作者： Pasquale Picone Domenico nuzzo Istituto per la Ricerca e l’Innovazione Biomedica CNRDipartimento di Scienze e Tecnologie Biologiche Chimiche e FarmaceuticheUniversitàdi PalermoPalermoItaly

Background:Nanotechnologies promise to improve disease diagnosis and treatment,overcoming the limitations of conventional *** particular,extracellular vesicles(EVs)and artificial vesicles(AVs)are strongly emerging tools in nanomedicine(Leggio et al.,2020).EVs are cell-derived membrane structures secreted after the fusion of endosomes with the plasma membrane(exosomes)or shed from the plasma membrane(microvesicles).EVs are released by different brain cells(neurons,oligodendrocytes,astrocytes,and microglia)and constitute a physiological intercellular communication ***,EVs can deliver different types of molecules(nucleic acids and proteins),which often influence the phenotype of the recipient *** play a physiological role in the central nervous system(CNS),such as development,myelination,regeneration,and synaptic activity(Lai et al.,2012).Due to their content,EVs could therefore constitute an important biomarker for neurodegenerative diseases,represent candidates for therapeutic use,enclosing regulatory molecules,or be considered as vectors for brain drug delivery(Croese et al.,2018).

关键词： diseases diagnosis constitute

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Estrogen/Huntingtin:a novel pathway involved in neuroprotection

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Neural Regeneration Research 2016年第3期11卷 402-403页

作者： Maria Teresa nuzzo Maria Marino Department of Science-Division of Biomedical Sciences and Technologies University Roma Tre

Neurodegenerative diseases（NDs）include more than 600 disease entities that are characterized by loss of specific neurons located in anatomically related functional areas which progressively lead to motor and cognitive *** pathogenesis of NDs involves mitochondrial dysfunction/oxidative stress,programmed cell death or abnormal protein aggregation,trafficking,and/or *** most cases,

关键词： programmed mitochondrial pathogenesis progressively aggregation oxidative abnormal neuronal caspase physiological

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BCLC staging system and liver transplantation:From a stage to a therapeutic hierarchy

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Hepatobiliary & Pancreatic Diseases International 2021年第1期20卷 4-5页

作者： Quirino Lai Alessandro vitale Hepato-biliary and Organ Transplant Unit Department of SurgerySapienza University of RomeViale del Policlinico 15500161 RomeItaly Department of Surgery Oncology and GastroenterologyUniversity of PaduaPaduaItaly

The Barcelona Clinic Liver Cancer(BCLC)system was proposed in 1999 with the intent to improve a therapeutic algorithm for the management of patients with hepatocellular carcinoma(HCC)[1].Both the European and the American Guidelines on the Treatment of HCC have endorsed the BCLC as the standard staging algorithm with prognostic and therapeutic implications[2,3].The BCLC staging system stratifies HCC patients into five stages(0,A,B,C and D).According to the algorithm,liver transplantation(LT)is indicated only in patients in the stages BCLC 0 and A,special situations provided.

关键词： therapeutic staging liver

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Application of machine learning techniques to simulate the evaporative fraction and its relationship with environmental variables in corn crops

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Ecological Processes 2022年第1期11卷 758-771页

作者： Terenzio Zenone Luca vitale Daniela Famulari Vincenzo Magliulo National Research Council Institute for Agricultural and Forestry Systems in the MediterraneanP.Le Enrico Fermi 180055 PorticiItaly National Research Council CNR Institute for BioeconomyVia P.Gobetti10140129 BolognaItaly

Background:The evaporative fraction(EF)represents an important biophysical parameter reflecting the distribution of surface available *** this study,we investigated the daily and seasonal patterns of EF in a multi-year corn cultivation located in southern Italy and evaluated the performance of five machine learning(ML)classes of algorithms:the linear regression(LR),regression tree(RT),support vector machine(SVM),ensembles of tree(ETs)and Gaussian process regression(GPR)to predict the EF at daily time *** adopted methodology consisted of three main steps that include:(i)selection of the EF predictors;(ii)comparison of the different classes of ML;(iii)application,cross-validation of the selected ML algorithms and comparison with the observed ***:Our results indicate that SVM and GPR were the best classes of ML at predicting the EF,with a total of four different algorithms:cubic SVM,medium Gaussian SVM,the Matern 5/2 GPR,and the rational quadratic *** com-parison between observed and predicted EF in all four algorithms,during the training phase,were within the 95%confidence interval:the R^(2)value between observed and predicted EF was 0.76(RMSE 0.05)for the medium Gaussian SVM,0.99(RMSE 0.01)for the rational quadratic GPR,0.94(RMSE 0.02)for the Matern 5/2 GPR,and 0.83(RMSE 0.05)for the cubic SVM *** results were obtained during the testing *** results of the cross-validation analysis indicate that the R^(2)values obtained between all iterations for each of the four adopted ML algorithms were basically constant,confirming the ability of ML as a tool to predict ***:ML algorithms represent a valid alternative able to predict the EF especially when remote sensing data are not available,or the sky conditions are not *** application to different geographical areas,or crops,requires further development of the model based on different data sources of soils,climate,and cropping systems.

关键词： Energy flux Evapotranspiration Eddy covariance Artificial intelligence

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Effects of calibration process on the simulation of rear-end conflicts at roundabouts

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Journal of Traffic and Transportation Engineering(English Edition) 2019年第2期6卷 175-184页

作者： Vincenzo Gallelli Giuseppe Guido Alessandro vitale Rosolino Vaiana Department of Civil Engineering University of Calabria

A methodology for calibrating and validating VISSIM simulation model is presented that allows to replicate the observed vehicles conflicts. A roundabout case study has been selected to test the usefulness of a combined approach of VISSIM simulation package and the surrogate safety assessment model(SSAM) for providing reliable estimates of traffic conflicts. Safety performance has been assessed from the field by video-recording vehicle interactions at the roundabout, and then expressed in terms of time to collision(TTC)*** proposed calibration procedure has been performed by a multistage methodology involving microscopic drivers' car following behavior parameters to enhance the correlation between observed and simulated queue lengths at the roundabout's entries. The calibration procedure is based on a statistical screening of inputs leading to a linear expression relating significant parameters to the queue length. The best estimates of the model's parameters have been determined using a genetic algorithm *** spatial distribution of the rear-end conflicts and the TTC values determined by SSAM have been finally compared with the observed ones to analyze the capability of the model of replicating rear-end *** results suggest to this calibration procedure impacts positively on the estimate of the safety performance measures obtained through the simulation *** the good results in the evaluation of the model's accuracy, the simulation seems to fail in reproducing the traffic phenomena linked to unusual driving behavior, and therefore it is not able to replicate forced drivers' maneuvers that can lead to a conflict situation.

关键词： Simulation Safety performance Unmanned aerial vehicle Roundabouts Traffic conflicts

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