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中华医学会疼痛学分会第十一届学术年会

作者： Xiuli Zhao Zongxiang Tang Hongkang Zhang Fidelis E Atianjoh Jian-Yuan Zhao Lingli Liang Wei Wang Xiaowei Guan Sheng-Chin Kao vinod tiwari Yong-Jing Gao Paul N Hoffman Hengmi Cui Min Li Xinzhong Dong Yuan-Xiang Tao Department of Anesthesiology and Critical Care Medicine Johns Hopkins University School of Medicine College of Basic Medicine Nanjing University of Chinese Medicine The Solomon H.Snyder Department of Neuroscience Johns Hopkins University School of Medicine Institute of Nautical Medicine Jiangsu Key Laboratory of Neuroregeneration Nantong University Department of Ophthalmology Johns Hopkins University School of Medicine Biomedical Innovation Institute Yangzhou University Howard Hughes Medical Institute Johns Hopkins University School of Medicine

＜正＞Objective:Neuropathic pain is a refractory disease characterized by maladaptive changes in gene transcription and translation in the sensory *** noncoding RNAs（lncRNAs） are emerging as new players in gene regulation,but how lncRNAs operate in the development of neuropathic pain is ***:Expressions of Kcna2 antisense RNA were examined using RT-PCR,Northern blot and in situ

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A set of SCAR markers in cluster bean (<i>Cyamopsis tetragonoloba</i>L. Taub) genotypes

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Advances in Bioscience and Biotechnology 2014年第2期5卷 131-141页

作者： Pratibha Sharma vinod Kumar K. Venkat Raman Kapil tiwari Department of Bioscience & Biotechnology Banasthali University Rajasthan India National Research Center on Plant Biotechnology IARI New Delhi India

Cluster bean, one of the most important cash legume crop has played an increasingly important role in wide range of industries. Owing to the significance of molecular marker studies in numerous applications including in genetic improvement of crops, there is an obvious need to undertake such studies in cluster bean. In the present work, 35 genotypes of cluster bean were collected from different states of India and analyzed using RAPD and ISSR markers. Further SCAR marker system was introduced in order to increase the reproducibility of the polymorphism and specificity. For this polymorphic (RP-3, 1000 bp;RP-19, 1250 bp and 1100 bp) and geographical specific bands (RP-9, 650 bp) from RAPD as well as genotype specific band (IS-8, 550 bp) from genotype RGC-1031 (Rajasthan) from ISSR were selected and converted into SCAR markers. The study revealed first set of sequence-based SCAR markers in cluster bean which found more specific information using RAPD and ISSR profiles. One genotype specific SCAR-20 for RGC-1031 (tolerant genotype against Macrophomina phaseolina), could be used to prove identity of the genotype for improvement as well for its genetic purity assessment. Another SCAR-8 was selected due to its specificity for cluster bean genotypes from Rajasthan which might be important for population admixture studies.

关键词： Cluster Bean ISSR RAPD SCAR

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