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Spermine induced reversible collapse of deoxyribonucleic acid-bridged nanoparticle-based assemblies

Nano Research 2018年第1期11卷 383-396页

作者： Kristian L. Goeken Richard B. M. Schasfoort vinod subram.niam.ron gill MIRA Institute for Biomedical Technology and Technical Medicine University of Twente Dfienerlolaan 5 7522 NB Enschede the Netherlands Vrije UniversiteitAmsterdam De Boelelaan 1105 1081 HVAmsterdam the Netherlands Saxion University of Applied Sciences M. H. Tromplaan 28 7513 A8 Enschede the Netherlands

DNA-linked 2D and 3D nano-assemblies find use in a diverse set of applications, ranging from DNA-origami in drug delivery and medical imaging, to DNA-linked nanoparticle structures for use in plasmonics and （bio）sensing. However, once these structures have been fully assembled, few options are available to modulate structure geometry. Here, we investigated the use of the polycation spermine to induce DNA collapse in small oligonucleotide-linked （54 bp） gold nanoparticle structures by monitoring shifts in the localized surface plasmon resonance （LSPR） peak and by comparing the data with finite-difference time-domain （FDTD） simulations. Our data shows that low concentrations of spermine can be applied to induce large changes in DNA conformation, leading to a significant reduction in interparticle distance （from - 25 to - 3 nm） and enhanced plasmonic coupling. The DNA collapse is near-instantaneous and reversible, and its application at low and high DNA densities is demonstrated with surface plasmon resonance imaging （SPRi）, showing the potential of spermine to dynamically modulate distances and geometry in DNA-based nano-assemblies.

关键词： nanoparticles spermine deoxyribonucleic acid localized surface plasmonresonance surface plasmon resonanceimaging

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Biodiversity,current developments and potential biotechnological applications of phosphorus-solubilizing and-mobilizing microbes:A review

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Pedosphere 2021年第1期31卷 43-75页

作者： Divjot KOUR Kusam Lata RANA Tanvir KAUR Neelam YADAV Ajar Nath YADAV Manish KUMAR vinod KUMAR Harcharan Singh DHALIWAL Anil Kumar SAXENA Department of Biotechnology Dr.Khem Singh Gill Akal College of AgricultureEternal UniversityBaru SahibSirmour 173101(India Gopi Nath P.G.College Veer Bahadur Singh Purvanchal UniversityGhazipur 275201(India Amity Institute of Biotechnology Amity UniversityGwalior 474005(India Biochemistry Forage SectionCollege of AgricultureCCS Agricultural UniversityHaryana 125004India ICAR-National Bureau of Agriculturally Important Microorganisms Kusmaur 275103(India)

As one of the most important and essential macronutrients next to nitrogen,phosphorus(P)is important for plant development,but it is the least mobile nutrient element in plant and ***,P is mined from geological sediments and added to agricultural soils so as to meet the critical requirements of crop plants for agronomic *** exists in soil in both organic and inorganic *** various inorganic forms of the element in soil are salts with calcium,iron,and aluminum,whereas the organic forms come from decaying vegetation and microbial *** is a huge diversity of plant microbiomes(epiphytic,endophytic,and rhizospheric)and soil microbiomes that have the capability to solubilize the insoluble P and make it available to *** main mechanism for the solubilization of inorganic P is by the production of organic acids,which lowers soil pH,or by the production of acid and alkaline phosphatases,which causes the mineralization of organic *** P-solubilizing and-mobilizing microorganisms belong to all three domains,comprising archaea,bacteria,and *** strains belonging to the genera Arthrobacter,Bacillus,Burkholderia,Natrinema,Pseudomonas,Rhizobium,and Serratia have been reported as efficient and potential P *** use of P solubilizers,alone or in combination with other plant growth-promoting microbes as an eco-friendly microbial consortium,could increase the P uptake of crops,increasing their yields for agricultural and environmental sustainability.

关键词： crop improvement gene expression microbial diversity organic acid production P mineralization P solubilizer phosphatase production plant growth-promoting traits

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肾功能和疾病规范命名:改善全球肾脏病预后(KDIGO)会议共识

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临床儿科杂志 2022年第8期40卷 627-633页

作者：王忍高春林夏正坤张沛(译) 东部战区总医院儿科江苏南京210002

为规范描述肾功能和疾病的专业术语,2019年6月改善全球肾脏病预后组织(KDIGO)召开共识会议,对肾脏功能和疾病、肾衰竭、急性肾脏病和急性肾损伤、慢性肾脏病及肾脏评估共5个部分的内容制定了相应的术语表,并就以下建议达成共识。①对于... 详细信息

为规范描述肾功能和疾病的专业术语,2019年6月改善全球肾脏病预后组织(KDIGO)召开共识会议,对肾脏功能和疾病、肾衰竭、急性肾脏病和急性肾损伤、慢性肾脏病及肾脏评估共5个部分的内容制定了相应的术语表,并就以下建议达成共识。①对于肾脏功能和疾病的一般描述,使用术语“kidney”,而不使用“renal”或前缀“nephro-”;②使用“肾衰竭(kidney failure)”并适当描述是否存在相应症状、体征和治疗,而不是“终末期肾脏疾病(end-stage kidney disease)”;③使用KDIGO对急性肾脏病(AKD)和急性肾损伤(AKI)进行定义和分类;④使用KDIGO对慢性肾脏病(CKD)进行定义和分类;⑤使用特定的肾脏检测指标,例如蛋白尿或肾小球滤过率(GFR)降低,而不是肾功能“异常(abnormal)”或“降低(reduced)”来描述肾脏结构和功能的改变。文章简要总结会议的主要讨论。

关键词：肾脏功能肾脏疾病术语

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