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All-epiphyseal versus trans-epiphyseal screw fixation for tillaux fractures:Does it matter?

World Journal of Orthopedics 2022年第2期13卷 131-138页

作者： Brett Heldt Isaiah Roepe Raymond Guo Elsayed Attia Ifeoma Inneh vinitha shenava Indranil Kushare Department of Orthopedic Surgery Baylor College of MedicineHoustonTX 77030United States Department of Orthopedic Surgery Texas Children's HospitalHoustonTX 77030United States

BACKGROUND Tillaux fractures occur primarily in adolescents due to the pattern of physeal closure and are classified as Salter-Harris type III physeal *** management with screw fixation is recommended for more than 2 mm of displacement or more than 1 mm of ***,the efficacy and complications of trans-physeal vs all-physeal screw fixation have not been investigated *** To compare the clinical and functional outcomes of trans-physeal(oblique)and all-epiphyseal(parallel)screw fixation in management of Tillaux fractures among pediatric *** This was an ethics board approved retrospective review of pediatric patients who presented to our tertiary children’s care facility with Tillaux *** included patients who had surgical fixation of a Tillaux fracture over a 10 year *** analysis included demographics,mode of injury,management protocols,and functional *** patients were divided into group 1(oblique fixation)and group 2(parallel fixation).Baseline patient characteristics and functional outcomes were compared between *** tests to evaluate differences included Fisher’s Exact or Chi-squared and independent samples t or Mann Whitney tests for categorical and continuous variables,*** A total of 42 patients(28 females and 14 males)were *** were no significant differences in body mass index,sex,age,or time to surgery between the groups[IK2].Sports injuries accounted for 61.9%of the cases,particularly non-contact(57.1%)and skating(28.6%)*** Tomography(CT)scan was ordered for 28 patients(66.7%),leading to diagnosis confirmation in 17 patients and change in management plan in 11 patients.[GRC3]Groups 1 and 2 consisted of 17 and 25 patients,*** mid to long-term functional outcomes,there were 14 and 10 patients in groups 1 and 2,*** analysis revealed no significant differences in the functional outcomes,pain scores,

关键词： Tillaux fracture Orthopedic surgery Fixation technique Functional outcomes

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Prospects for discriminating Zingiberaceae species in India using DNA barcodes

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Journal of Integrative Plant Biology 2014年第8期56卷 760-773页

作者： Meenakshi Ramaswamy vinitha Unnikrishnan Suresh Kumar Kizhakkethil Aishwarya Mamiyil Sabu George Thomas Plant Molecular Biology Rajiv Gandhi Centre for BiotechnologyThiruvananthapuram Kerala 695014 India Regional Facility for DNA Fingerprinting Rajiv Gandhi Centre for BiotechnologyThiruvananthapuram Kerala 695014 India Department of Botany University of Calicut

We evaluated nine plastid （matK, rbcL, rpoCl, rpoB, rp136-rpsS, ndhJ, trnL-F, tmrnH-psbA, accD） and two nuclear （ITS and ITS2） barcode loci in family Zingiberaceae by analyzing 60 accessions of 20 species belonging to seven genera from India. Bidirectional sequences were recovered for every plastid locus by direct sequencing of polymerase chain reaction （PCR） amplicons in all the accessions tested. However, only 35 （58%） and 4o accessions （66~） yielded ITS and ITS2 sequences, respectively, by direct sequencing. In different bioinformatics analyses, matK and rbcL consistently resolved 15 species （75%） into monophyletic groups and five species into two para- phyletic groups. The 173 ITS sequences, including 138 cloned sequences from 23 accessions, discriminated only 12 species （6o%）, and the remaining species were entered into three paraphyletic groups. Phylogenetic and genealogic analyses of plastid and ITS sequences imply the possible occurrence ofnatural hybridizations in the evolutionary past in giving rise to species paraphyly and intragenomic ITS heterogeneity in the species tested. The results support using matK and rbcL loci for barcoding Zingiberaceae members and highlight the poor utility of iTS and the highly regarded ITS2 in barcoding this family, and also caution against proposing ITS loci for barcoding taxa based on limited sampling.

关键词： Concerted evolution DNA barcoding ITS heterogeneity natural hybridizations Zingiberaceae

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A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

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Microsystems & Nanoengineering 2020年第1期6卷 1316-1333页

作者： Sthitodhi Ghosh Kashish Aggarwal vinitha T.U Thinh Nguyen Jungyoup Han Chong H.Ahn Department of Electrical Engineering and Computer Science Microsystems and BioMEMS LaboratoryUniversity of CincinnatiCincinnatiOH 45221USA Mico BioMed USA Inc. 10999 Reed Hartman HighwaySTE 309CCincinnatiOH 45242USA

There has been a considerable development in microfluidic based immunodiagnostics over the past few years which has greatly favored the growth of novel point-of-care-testing(POCT).However,the realization of an inexpensive,low-power POCT needs cheap and disposable microfluidic devices that can perform autonomously with minimum user *** work,for the first time,reports the development of a new microchannel capillary flow assay(MCFA)platform that can perform chemiluminescence based ELISA with lyophilized chemiluminescent *** new MCFA platform exploits the ultra-high sensitivity of chemiluminescent detection while eliminating the shortcomings associated with liquid reagent handling,control of assay sequence and user *** functionally designed microchannels along with adequate hydrophilicity produce a sequential flow of assay reagents and autonomously performs the ultra-high sensitive chemiluminescence based ELISA for the detection of malaria biomarker such as *** MCFA platform with no external flow control and simple chemiluminescence detection can easily communicate with smartphone via USB-OTG port using a custom-designed optical *** use of the smartphone for display,data transfer,storage and analysis,as well as the source of power allows the development of a smartphone based POCT analyzer for disease *** paper reports a limit of detection(LOD)of 8 ng/mL by the smartphone analyzer which is sensitive enough to detect active malarial *** MCFA platform developed with the smartphone analyzer can be easily customized for different biomarkers,so a hand-held POCT for various infectious diseases can be envisaged with full networking capability at low cost.

关键词： POCT smart capillary

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Secretive derived from hypoxia preconditioned mesenchymal stem cells promote cartilage regeneration and mitigate joint inflammation via extracellular vesicles

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Bioactive Materials 2023年第9期27卷 98-112页

作者： Yanmeng Yang Yingnan Wu Dahou Yang Shu Hui Neo Nurul Dinah Kadir Doreen Goh Jian Xiong Tan vinitha Denslin Eng Hin Lee Zheng Yang Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of SingaporeSingapore NUS Tissue Engineering Program Life Sciences InstituteNational University of SingaporeSingapore Critical Analytics for Manufacturing Personalised-Medicine Singapore-MIT Alliance for Research and TechnologySingapore

Secretome derived from mesenchymal stem cells (MSCs) have profound effects on tissue regeneration, which could become the basis of future MSCs therapies. Hypoxia, as the physiologic environment of MSCs, has great potential to enhance MSCs paracrine therapeutic effect. In our study, the paracrine effects of secretome derived from MSCs preconditioned in normoxia and hypoxia was compared through both in vitro functional assays and an in vivo rat osteochondral defect model. Specifically, the paracrine effect of total EVs were compared to that of soluble factors to characterize the predominant active components in the hypoxic secretome. We demonstrated that hypoxia conditioned medium, as well as the corresponding EVs, at a relatively low dosage, were efficient in promoting the repair of critical-sized osteochondral defects and mitigated the joint inflammation in a rat osteochondral defect model, relative to their normoxia counterpart. In vitro functional test shows enhancement through chondrocyte proliferation, migration, and matrix deposition, while inhibit IL-1β-induced chondrocytes senescence, inflammation, matrix degradation, and pro-inflammatory macrophage activity. Multiple functional proteins, as well as a change in EVs’ size profile, with enrichment of specific EV-miRNAs were detected with hypoxia preconditioning, implicating complex molecular pathways involved in hypoxia pre-conditioned MSCs secretome generated cartilage regeneration.

关键词： Hypoxia preconditioned MSCs Secretome Extracellular vesicles Cartilage regeneration Joint inflammation

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Evaluation of the Toxic Effects of Heavy Metals and Endocrine-Disrupting Chemicals to the Marine Dinoflagellate Prorocentrum minimum

Evaluation of the Toxic Effects of Heavy Metals and Endocrin...

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2012年环境污染与大众健康学术会议

作者： vinitha Ebenezer Jang-Seu Ki Department of Green Life Science Sangmyung University Seoul-110-743 South Korea

Microalgae are bioindicators for risk assessment and development of environmental regulations for heavy metals and other emerging toxicants in marine environments. In this study, we evaluated the toxic effects of the heavy metals Cd, Pb, and Ni, and the endocrine disrupting chemicals （EDCs） polychlorinated biphenyl （PCB）, endosulfan （ES）, and metolachlor （MC） on the marine dinoflagellate Prorocentrum minimum. Toxicity assays showed that cell count and chlorophyll a level decreased with increased toxicant concentrations in all cases. EC 50 -72 h for the test chemicals were as follows: Cd, 2.78 mg L -1;Pb, 3.49 mg L -1;Ni, 0.64 mg L -1;PCB, 8.0 μg L -1;ES, 0.025 mg L -1;MC, 0.079 mg L -1 . These data suggested that the sensitivity pattern of P. minimum to heavy metals was Ni ＞ Cd ＞ Pb;three EDCs were mostly sensitive, and the PCB was highly toxic. This study provides essential data on the toxic effects of heavy metals and newly introduced EDCs on marine dinoflagellate.

关键词： Dinoflagellate Prorocentrum ecotoxicity assessment EC50 , heavy metals EDCs

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Effect of Substituent Position on the Properties of Chalcone Isomer Single Crystals

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Journal of Crystallization Process and Technology 2013年第4期3卷 148-155页

作者： R. Gandhimathi G. vinitha R. Dhanasekaran Crystal Growth Centre Anna University Chennai India 2Division of Physics Vellore Institute of Technology Chennai India.

This paper summarizes the synthesis, growth and the effect of the position of the substituent in the thienyl ring and also the properties of the grown chalcone crystals, 2-CTP and 3-CTP. The formation of compound is confirmed by the re- corded H1NMR spectra. A FT-IR spectrum confirms the presence of all functional groups in both of the crystals. Single crystal XRD reports that even though these two compounds crystallize in monoclinic crystal system, 2-CTP has centro- symmetric P21/c space group and 3-CTP has non-centrosymmetric space group P21. Thermal properties of grown crys- tals analyzed by TG/DTA study explain that the 3-CTP compound is slightly more stable than 2-CTP. The transparency of these isomers in the vis-IR region has been studied. Second and third order nonlinear optical properties of 3-CTP and 2-CTP crystals have been investigated by powder SHG and Z-scan technique respectively.

关键词： Solution Growth Characterization Chalcones Nonlinear Optical Materials

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The Impact of Human Immunodeficiency Virus Infection (HIV) on Lymphoma in South Africa

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Journal of Cancer Therapy 2015年第6期6卷 527-535页

作者： Moosa Patel vinitha Philip Tanvier Omar Dianne Turton Geoff Candy Atul Lakha Sugeshnee Pather Clinical Haematology Unit Department of Medicine Chris Hani Baragwanath Academic Hospital (CHBAH) Johannesburg South Africa Department of Anatomical Pathology National Health Laboratory Services (NHLS) Johannesburg South Africa Ampath Laboratories Johannesburg (Previously Department of Anatomical Pathology NHLS) South Africa Department of Surgery University of the Witwatersrand Johannesburg South Africa and Faculty of Health Sciences University of the Witwatersrand Johannesburg South Africa

Human immunodeficiency virus (HIV) infection is endemic in South Africa. Non-Hodgkin lymphoma (NHL) occurs with increased frequency in HIV seropositive individuals. The increase in NHL has been more marked in the last decade, with HIV being the major contributor to this increase. More than 70% of the adult NHL patients at Chris Hani Baragwanath Academic Hospital (CHBAH), Soweto, Johannesburg, are HIV seropositive. In addition, HIV has impacted on the clinical presentation—being more aggressive and atypical. Histologically, HIV-NHL typically manifests as B-cell, high grade lymphomas, including diffuse large B-cell lymphoma (DLBCL);Burkitt lymphoma (BL);B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and BL and plasmablastic lymphoma. The latter two entities, which were previously rare or unknown, have gained prominence in the last decade, occurring primarily in HIV seropositive individuals. HIV-NHL, being associated with all these adverse prognostic factors results in a poorer overall survival.

关键词： Human Immunodeficiency Virus (HIV) Non-Hodgkin Lymphoma (NHL) South Africa High Grade Adverse Prognostic Factors Poorer Overall Survival

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Signal Processing and AI-based Assessment of Rehabilitation Exercises for Diastasis Recti Abdominis

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Computer Systems Science & Engineering 2023年第10期47卷 333-348页

作者： R.Karthik R.Menaka P.Ponmathi Daehan Won P.vinitha Joshy J.G.Aravindan S.Harshavardhan K.V.S.D.Aashish kumar R.Akileshkumar Centre for Cyber Physical Systems(CCPS) School of Electronics EngineeringVellore Institute of TechnologyChennai600127India Faculty of Physiotherapy Sri Ramachandra Institute of Higher Education and ResearchChennai600116India System Sciences and Industrial Engineering Binghamton UniversityBinghamton13902-6000USA School of Electronics Engineering Vellore Institute of TechnologyChennai600127India

Diastasis Recti Abdominis(DRA)is the separation of abdominal recti muscles which occurs in women during their pregnancy and postpartum *** is because of the stretching of the linea alba,a fibrous connective tissue on the abdominal *** Linea Alba is elastic and retracts back after the delivery of the *** this tissue gets overstretched,it loses its elasticity and the gap in the abdominals may not be closed leading to *** motive of this research is to analyze the postpartum rehabilitation for signals from Inertial Measurement Unit(IMU)*** conservative treatment for women who are experiencing DRA is given in the form of *** physiotherapy exercises focus on the recti abdominis muscle to bring back the Linea alba *** will be a difficult process for the physiotherapist to monitor,whether patients did the exercises correctly or *** the exercises are not correct,they will not be effective in the reduction of inter-recti *** research aims to analyze the effectiveness of IMU signals in classifying the correct and incorrect *** was inferred that the IMU signals are effective in classifying the correct and incorrect exercises with an accuracy of 92%.

关键词： DRA IMU inter-recti distance rehabilitation machine learning

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Breast imaging:A survey

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World Journal of Clinical Oncology 2011年第4期2卷 171-178页

作者： Subbhuraam vinitha Sree Eddie Yin-Kwee Ng Rajendra U Acharya Oliver Faust School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore Department of Electronics and Computer Engineering Ngee Ann PolytechnicSingapore 599489Singapore

Breast cancer is the second leading cause of death in *** occurs when cells in the breast start to grow out of proportion and invade neighboring tissues or spread throughout the *** is one of the most effective and popular modalities presently used for breast cancer screening and *** have been made to improve the accuracy of breast cancer diagnosis using different imaging *** and magnetic resonance imaging have been used to detect breast cancers in high risk ***,electrical impedance imaging and nuclear medicine techniques are also being widely used for breast cancer screening and *** this paper,we discuss the capabilities of various breast imaging modalities.

关键词： Breast cancer Breast magnetic resonance imaging Breast ultrasound Mammography Thermography

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