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Woody plant cell walls:Fundamentals and utilization

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Molecular Plant 2024年第1期17卷 112-140页

作者： Wei li Ying-Chung Jimmy lin Ying-Lan Chen Chenguang Zhou Shuang li Nette De Ridder Dyoni M.Oliveira Lanjun Zhange Baocai Zhang Jack P.Wang Changzheng Xu Xiaokang Fu Keming Luo Ai-Min Wu Taku Demura Meng-Zhu Lu Yihua Zhou Laigeng li Toshiaki Umezawa Wout Boerjan vincent L.Chiang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040China Department of life Science National Taiwan UniversityTaipeiTaiwanChina Department of Biotechnology and Bioindustry Sciences College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainanTaiwanChina Department of Plant Biotechnology and Bioinformatics Ghent UniversityTechnologiepark 719052 GhentBelgium VIB Center for Plant Systems Biology Technologiepark 719052 GhentBelgium State Key Laboratory of Plant Genomics Insttute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation Integrative Science Center of Germplasm CreationSchool of Life SciencesSouthwest UniversityChongqing 400715China Guangdong Key Laboratory for Innovative Developmentand Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitecturesSouth China Agricultural UniversityGuangzhou510642China Center for Digital Green-innovation Nara Institute of Science and TechnologykomaNara 630-0192Japan State Key Laboratory of Subtropical Siviculture College of Forestry and BiotechnologyZhejang A&F UniversityHangzhou 311300China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences300 Fenglin RoadShanghai 200032China Laboratoryof MetabolicScienceofForest Plants and Microorganisms Rsearch Institute for Sustainable HumanosphereKyoto UniversityUjiKyoto 611-0011 Japan

Cell walls in plants,particularly forest trees,are the major carbon sink of the terrestrial *** and biosynthetic features of plant cell walls were revealed early on,focusing mostly on herbaceous model *** developments in genomics,transcriptomics,epigenomics,transgenesis,and associated analytical techniques are enabling novel insights into formation of woody cell ***,we review multilevel regulation of cell wall biosynthesis in forest tree *** highlight current approaches to engineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic *** outline opportunities and challenges in future research to better understand cell type biogenesis for more efficient wood cell wall modification and utilization for biomaterials or for enhanced carbon capture and storage.

关键词： regulation of cell wall formation biosynthesis of cell wall components lignin cellulose hemicellulose cell wall utilization

科学论文向技术领域扩散的扩散速度与扩散效果研究

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情报理论与实践 2024年第7期47卷 35-47页

作者：李冰丁堃孙晓玲江文森大连理工大学科学学与科技管理研究所辽宁大连116024 蒙特利尔大学加拿大蒙特利尔H3C 3J7

[目的/意义]科学论文向技术领域扩散对技术的创新发展发挥着重要作用,然而论文在技术领域的扩散速度参差不齐,扩散速度的快慢是否影响产生的扩散效果尚不清晰。[方法/过程]文章利用专利引用论文的引用关系,以1980—2009年Web of Scienc... 详细信息

[目的/意义]科学论文向技术领域扩散对技术的创新发展发挥着重要作用,然而论文在技术领域的扩散速度参差不齐,扩散速度的快慢是否影响产生的扩散效果尚不清晰。[方法/过程]文章利用专利引用论文的引用关系,以1980—2009年Web of Science数据库的论文和1980—2020年USPTO数据库的授权专利作为核心数据集,通过论文被引速率和首次被引间隔测度论文向技术领域的扩散速度,通过扩散产生的技术影响强度和技术影响广度综合测度扩散效果,并添加被引间隔和被引速率的交互作用,分析论文扩散速度与扩散效果的关系。此外,选择被专利持续引用的论文作为研究对象,分析不同扩散时期的扩散速率与扩散效果的关系。[结果/结论]研究表明,论文整体扩散速率不断上升;扩散速率与技术影响强度和广度均呈现倒U形关系,被引间隔与技术影响强度和广度均呈现U形关系;被引间隔对扩散速率与扩散效果的关系具有显著调节作用。此外,在不同扩散时期,早期和中期的扩散速率与技术影响强度和广度呈现倒U形关系,而中后期扩散速率与技术影响强度和广度呈现U形关系。

关键词：论文扩散扩散速度技术影响强度技术影响广度专利引用论文

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LBD12转录因子调控毛果杨木材形成研究

南京林业大学学报（自然科学版） 2024年第1期48卷 29-38页

作者：高源孙佳彤周晨光姜立泉李伟李爽林木遗传育种全国重点实验室(东北林业大学) 黑龙江哈尔滨150040 辽宁省森林经营研究所辽宁丹东118000

【目的】创制毛果杨(Populus trichocarpa)LBD12(lateral organ boundaries domain 12)转录因子的过量表达植株,分析表型特征,初步探究PtrLBD12转录因子在毛果杨木材形成中的生物功能。【方法】基于前期的研究,筛选出了木材形成关键调... 详细信息

【目的】创制毛果杨(Populus trichocarpa)LBD12(lateral organ boundaries domain 12)转录因子的过量表达植株,分析表型特征,初步探究PtrLBD12转录因子在毛果杨木材形成中的生物功能。【方法】基于前期的研究,筛选出了木材形成关键调控因子PtrbHLH186调控的下游转录因子PtrLBD12,通过基因克隆获得PtrLBD12基因;创制PtrLBD12过量表达毛果杨植株,测定并统计生长30、60、90 d毛果杨野生型和过表达植株的生长指标;对生长120 d的转基因及野生型植株各茎节进行石蜡切片、化学染色观察及不同细胞类型的统计分析;通过定量PCR测定过表达PtrLBD12基因对22个木质素单体合成酶基因表达水平的影响。【结果】获得毛果杨PtrLBD12过表达植株;与野生型相比,转基因植株的株高、地径等生长指标水平明显降低;纤维和导管细胞的数量显著增多,导管细胞的孔径减小,形成层细胞的形态结构无明显变化;木质素沉积水平增加,木质化程度增强;PtrLBD12的过量表达促进了木质素合成酶基因的表达。【结论】LBD12转录因子在毛果杨木材形成过程中发挥重要的调控作用,可调节木质素单体合成酶基因的表达,改变木质素沉积方式。此外,该基因也可以改变木质部细胞形态结构,影响植株生长发育。

关键词：毛果杨木材形成遗传转化转录因子 PtrLBD12

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Carcinoid tumor of the appendix: A consecutive series from 1237 appendectomies

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World Journal of Gastroenterology 2006年第41期12卷 6699-6701页

作者： vincent Tchana-Sato Olivier Detry Marc Polus Albert Thiry Bernard Detroz Sylvie Maweja Etienne Hamoir Thierry Defechereux Carla Coimbra Arnaud De Roover Michel Meurisse Pierre Honoré Department of Abdominal Surgery and Transplantation University of LiègeCHU Sart Tilman B35Liège B4000Belgium Department of Gastroenterology University of LiègeCHU Sart Tilman B35Liège B4000Belgium Department of Pathology University of LiègeCHU Sart Tilman B35Liège B4000Belgium

AIM： To report the experience of the CHU Sart Tilman, University of liege, Belgium, in the management of appendiceal carinoid tumor. METHODS： A retrospective review of 1237 appendectomies performed in one single centre from January 2000 to May 2004, was undertaken. Analysis of demographic data, clinical presentation, histopathology, operative reports and outcome was presented. RESULTS： Among the 1237 appendectomies, 5 appendiceal carcinoid tumors were identified （0.4%） in 4 male and 1 female patients, with a mean age of 29.2 years （range： 6-82 years）. Acute appendicitis was the clinical presentation for all patients. Four patients underwent open appendectomy and one a laparoscopic procedure. One patient was reoperated to complete the excision of mesoappendix. All tumors were located at the tip of the appendix with a mean diameter of 0.6 cm （range： 0.3-1.0 cm）. No adjuvant therapy was performed. All patients were alive and disease-free during a mean follow-up of 33 mo. CONCLUSION： Appendiceal carcinoid tumor most of-ten presents as appendicitis. In most cases, it is found incidentally during appendectomies and its diagnosis is rarely suspected before histological examination. Appendiceal carcinoid tumor can be managed by simple appendectomy and resection of the mesoappendix, if its size is ≤ 1cm.

关键词： Surgery Digestive cancer Laparoscopy Review

Advances in Quantifying Impact and Extent of Water-Land-Food Nexus for Food Security in Africa

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Agricultural Sciences 2022年第4期13卷 520-541页

作者： Auguste Cesar Itangishaka Yonghui Yang Kiril Manevski Huilong li Telesphore Habiyakare Gratien Twagirayezu Jean Yves Uwamungu Christine Mushimiyimana Romaine Ingabire Hubert Hirwa vincent Nzabarinda Key Laboratory of Agricultural Water Resources Hebei Laboratory of Agricultural Water-Saving Center for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Shijiazhuang China University of Chinese Academy of Sciences Beijing China Department of Agroecology Aarhus University Tjele Denmark Sino-Danish Center for Education and Research Beijing China College of Resources and Environment Chinese Academy of Sciences Beijing China School of Environmental and Municipal Engineering Lanzhou Jiaotong University Lanzhou China Tianshui Normal University Tianshui China College of Bioengineering and Technology Tianshui Normal University Tianshui China Gansu Provincial Key Laboratory of Resource Utilization of Agricultural Solid Waste Tianshui Normal University Tianshui China China-Belarus Institute of Ecology and Environment Tianshui Normal University Tianshui China Key Laboratory of Ecosystem Network Observation and Modelling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi China

Food systems have the potential to promote human health and enhance environmental sustainability;yet most African countries have decadal starvation and malnourishment due to unstable food systems lacking basic nutritional profiles. To understand the key questions of the African food production and food security crisis, it is important to evaluate the past and the present balance of food production and consumption quantitatively and comprehensively and identify resource constraints. This continental study analyzed water-land-food (WLF) nexus in recent decades (1997-2017) and accessed whether these resources cope with different population growth projections, dietary changes and agricultural water management. The findings revealed that in 2017, total production in Africa for the four major crops was 83.3, 34.1, 21.0 and 26.7 million tons for, respectively, maize, rice, sorghum and wheat. Together with the imported food, 38 countries experienced an increase in kcal by food supply, while 7 countries encountered a decrease. However, only 6 countries were above the 1500 kcal∙capita−1∙day−1—the global average food consumption from the four major crops. The study also found that in the context of food production and out of the total African population, 268 million (21.6%) have enough land and water, 310 million (25.0%) is without enough land and water, further 279 million (22.5%) have enough water but not enough land, and 381 million (30.8%) encounter enough land but not enough water. This is the first WLF nexus study for the African continent and emphasizes the need for efficient and rapid changes in the food systems of the African population, both in production and consumption, in order to provide sustainable and secure food systems, and ultimately approach the first three Sustainable Development Goals.

关键词： Basic Dietary Requirements Cereal Import Dependency Ration Constraint Import Production Shortages Resource

Depletion of the IKBKAP ortholog in zebrafish leads to hirschsprung disease-like phenotype

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World Journal of Gastroenterology 2015年第7期21卷 2040-2046页

作者： William Wai-Chun Cheng Clara Sze-Man Tang Hong-Sheng Gui Man-Ting So vincent Chi-Hang Lui Paul Kwong-Hang Tam Maria-Mercè Garcia-Barcelo Department of Surgery Li Ka Shing Faculty of Medicine the University of Hong Kong Department of Psychiatry Li Ka Shing Faculty of Medicine the University of Hong Kong Center for Genomic Sciences of the li Ka Shing Faculty of Medicine the University of Hong Kong Centre for Reproduction Development and Growth Li Ka Shing Faculty of Medicine the University of Hong Kong

AIM:To investigate the role of IKBKAP(inhibitor of kappa light polypeptide gene enhancer in B-cells,kinase complexassociated protein)in the development of enteric nervous system(ENS)and Hirschsprung disease(HSCR).METHODS:In this study,we injected a morpholino that blocked the translation of ikbkap protein to 1-cell stage zebrafish *** phenotype in the ENS was analysed by antibody staining of the pan-neuronal marker Hu C/D followed by enteric neuron *** mean numbers of enteric neurons were compared between the morphant and the *** also studied the expressions of ret and phox2bb,which are involved in ENS development,in the ikbkap morpholino injected embryos by quantitative reverse transcriptase polymerase chain reaction and compared them with the ***:We observed aganglionosis(χ2,Plino(morphant)when compared with the control *** of the morpholino was confirmed by similar results obtained using a second non-overlapping morpholino that blocked the translation of *** further studied the morphant by analysing the expression levels of genes involved in ENS development such as ret,phox2bb and sox10,and found that phox2bb,the ortholog of human PHOX2B,was significantly down-regulated(0.51±0.15 vs 1.00±0,P<0.05).Although we also observed a reduction in theexpression of ret,the difference was not ***:Loss of IKBKAP contributed to HSCR as demonstrated by functional analysis in zebrafish embryos.

关键词： Hirschsprung disease Enteric nervous system IKBKAP

基于CRISPR/Cas9的毛果杨bHLH106转录因子的功能研究

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南京林业大学学报（自然科学版） 2021年第6期45卷 15-23页

作者：孙佳彤国艳娇李爽周晨光姜立泉李伟林木遗传育种国家重点实验室(东北林业大学) 黑龙江哈尔滨150040

【目的】采用CRISPR/Cas9基因编辑系统创制毛果杨(Populus trichocarpa)bHLH106(Basic Helix-Loop-Helix 106)基因的突变体,分析植株的表型特征,初步揭示PtrbHLH106基因在毛果杨木材形成过程中的功能。【方法】基于前期对毛果杨野生型(... 详细信息

【目的】采用CRISPR/Cas9基因编辑系统创制毛果杨(Populus trichocarpa)bHLH106(Basic Helix-Loop-Helix 106)基因的突变体,分析植株的表型特征,初步揭示PtrbHLH106基因在毛果杨木材形成过程中的功能。【方法】基于前期对毛果杨野生型(WT)茎干的不同细胞类型(形成层、木质部和韧皮部细胞)RNA-seq数据,克隆得到一个在形成层及木质部较高表达的bHLH基因PtrbHLH106。采用CRISPR/Cas9基因编辑技术创制毛果杨PtrbHLH106的功能缺失突变体。对生长60、90、120 d的毛果杨ptrbhlh106突变体和WT植株进行表型观察;对生长120 d的植株各茎节进行石蜡切片,利用甲苯胺蓝染色观察并进行细胞统计分析。【结果】获得毛果杨ptrbhlh106突变体;与WT相比,突变体植株的株高、地径无明显差异;在整个测量的生长周期中,第8茎节长度有缩短的趋势,茎节数量有增加的趋势;形成层细胞层数有增加的趋势但差异不显著,导管细胞孔径显著增大,纤维细胞数量显著减少。【结论】ptrbhlh106突变体与WT植株在导管孔径和纤维细胞数量上存在差异,初步证明PtrbHLH106基因参与了调控毛果杨次生木质部的发育。

关键词：毛果杨次生木质部发育 CRISPR/Cas9基因编辑 PtrbHLH106转录因子

基于CRISPR/Cas9的毛果杨PtrHBI1基因功能解析

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南京林业大学学报（自然科学版） 2021年第6期45卷 31-39页

作者：王竹雯国艳娇李爽周晨光姜立泉李伟林木遗传育种国家重点实验室(东北林业大学) 黑龙江哈尔滨150040

【目的】利用CRISPR/Cas9系统创制毛果杨PtrHBI1功能缺失突变体,初步解析该转录因子在木材形成过程中的功能,为利用基因工程手段培育制浆造纸优良性状的林木新品种提供新思路。【方法】以毛果杨(Populus trichocarpa)为研究材料,根据激... 详细信息

【目的】利用CRISPR/Cas9系统创制毛果杨PtrHBI1功能缺失突变体,初步解析该转录因子在木材形成过程中的功能,为利用基因工程手段培育制浆造纸优良性状的林木新品种提供新思路。【方法】以毛果杨(Populus trichocarpa)为研究材料,根据激光显微切割技术捕获的野生型毛果杨不同细胞类型(形成层、木质部和韧皮部)RNA-seq数据,筛选得到1个bHLH家族转录因子PtrHBI1,利用毛果杨木质部原生质体系统对该基因进行亚细胞定位分析,采用原位杂交技术分析PtrHBI1组织特异性表达,采用CRISPR/Cas9技术创制毛果杨ptrhbi1突变体。对ptrhbi1突变体进行生长表型分析,利用石蜡切片对茎段横切面的各细胞类型进行形态分析,利用Klason酸水解法检测突变体木质素含量,使用高效液相色谱测定突变体纤维素和半纤维素含量。【结果】亚细胞定位显示PtrHBI1基因定位于细胞核,原位杂交结果表明PtrHBI1主要在形成层和木质部表达。通过CRISPR/Cas9创制的毛果杨ptrhbi1突变体株高显著增加,茎节数和地径在一定时期显著大于野生型。此外,突变体的导管孔径显著增大,纤维细胞数量显著减少。导管细胞的数量和形成层细胞层数与野生型无差异。木材组分分析表明,与野生型植株相比,ptrhbi1植株的纤维素含量增加,木质素总量无显著变化,紫丁香基木质素与愈创木基木质素之质量比(S/G)显著降低。【结论】PtrHBI1参与调控毛果杨的生长及次生木质部发育,可能在木材形成过程起着较为重要的调控作用。

关键词：毛果杨木材形成调控 CRISPR/Cas9 PtrHBI1转录因子

Towards 6G wireless communication networks:vision, enabling technologies, and new paradigm shifts

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Science China(Information Sciences) 2021年第1期64卷 5-78页

作者： Xiaohu YOU Cheng-Xiang WANG Jie HUANG Xiqi GAO Zaichen ZHANG Mao WANG Yongming HUANG Chuan ZHANG Yanxiang JIANG Jiaheng WANG Min ZHU Bin SHENG Dongming WANG Zhiwen PAN Pengcheng ZHU Yang YANG Zening liU Ping ZHANG Xiaofeng TAO Shaoqian li Zhi CHEN Xinying MA Chih-lin I Shuangfeng HAN Ke li Chengkang PAN Zhimin ZHENG Lajos HANZO Xuemin (Sherman) SHEN Yingjie Jay GUO Zhiguo DING Harald HAAS Wen TONG Peiying ZHU Ganghua YANG Jun WANG Erik G.LARSSON Hien Quoc NGO Wei HONG Haiming WANG Debin HOU Jixin CHEN Zhe CHEN Zhangcheng HAO Geoffrey Ye li Rahim TAFAZOLli Yue GAO H.vincent POOR Gerhard P.FETTWEIS Ying-Chang liANG National Mobile Communications Research Laboratory School of Information Science and EngineeringSoutheast University Purple Mountain Laboratories Shanghai Institute of Fog Computing Technology (SHIFT) ShanghaiTech University Research Center for Network Communication Peng Cheng Laboratory State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications National Engineering Laboratory for Mobile Network Technologies Beijing University of Posts and Telecommunications National Key Laboratory of Science and Technology on Communications University of Electronic Science and Technology of China (UESTC) China Mobile Research Institute School of Electronics and Computer Science University of Southampton Department of Electrical and Computer Engineering University of Waterloo Global Big Data Technologies Centre (GBDTC) University of Technology Sydney School of Electrical and Electronic Engineering The University of Manchester liFi Research and Development Centre Institute for Digital CommunicationsSchool of EngineeringThe University of Edinburgh Huawei Technologies Canada Co. Ltd. Huawei Technologies Department of Electrical Engineering (ISY) Link?ping University Institute of Electronics Communications & Information Technology (ECIT)Queen's University Belfast State Key Laboratory of Millimeter Waves School of Information Science and EngineeringSoutheast University School of Electrical and Computer Engineering Georgia Institute of Technology 5G Innovation Centre University of Surrey Princeton University Vodafone Chair Mobile Communications Systems Technische Universit?t Dresden Center for Intelligent Networking and Communications (CINC) University of Electronic Science and Technology of China (UESTC)

The fifth generation(5G) wireless communication networks are being deployed worldwide from 2020 and more capabilities are in the process of being standardized, such as mass connectivity, ultra-reliability,and guaranteed low latency. However, 5G will not meet all requirements of the future in 2030 and beyond, and sixth generation(6G) wireless communication networks are expected to provide global coverage, enhanced spectral/energy/cost efficiency, better intelligence level and security, etc. To meet these requirements, 6G networks will rely on new enabling technologies, i.e., air interface and transmission technologies and novel network architecture, such as waveform design, multiple access, channel coding schemes, multi-antenna technologies, network slicing, cell-free architecture, and cloud/fog/edge computing. Our vision on 6G is that it will have four new paradigm shifts. First, to satisfy the requirement of global coverage, 6G will not be limited to terrestrial communication networks, which will need to be complemented with non-terrestrial networks such as satellite and unmanned aerial vehicle(UAV) communication networks, thus achieving a space-airground-sea integrated communication network. Second, all spectra will be fully explored to further increase data rates and connection density, including the sub-6GHz, millimeter wave(mmWave), terahertz(THz),and optical frequency bands. Third, facing the big datasets generated by the use of extremely heterogeneous networks, diverse communication scenarios, large numbers of antennas, wide bandwidths, and new service requirements, 6G networks will enable a new range of smart applications with the aid of artificial intelligence(AI) and big data technologies. Fourth, network security will have to be strengthened when developing 6G networks. This article provides a comprehensive survey of recent advances and future trends in these four aspects. Clearly, 6G with additional technical requirements beyond those of 5G will enable f

关键词： 6G vision network architecture air interface and transmission technologies space-air-ground-sea integrated network all spectra artificial intelligence network security.