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In silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species

Genes & Diseases 2018年第2期5卷 123-129页

作者： Abraham Peele Karlapudi Venkateswarulu T.C Jahnavi Tammineedi Krupanidhi Srirama Lohit Kanumuri vidya prabhakar kodali Department of Biotechnology Vignan’s Foundation for Science Technology and ResearchVadlamudiGuntur 522213India Department of Biotechnology Vikrama Simhapuri UniversityNelloreIndia

CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and to promote CRISPR adaptation,uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial population is at highest risk of phage *** baumannii is the most regularly identified gram-negative bacterium infecting *** reports have demonstrated that the extent of diseases caused by *** is expanding and,in a few cases,now surpasses the quantity of infections caused by *** Acinetobacter strains possess biofilm-forming ability,which plays a major role in virulence and drug *** bacteria use quorum sensing,a cell-to-cell communication process,to activate gene *** genes are involved in biofilm formation and the mechanism to disrupt the biofilm network is still not clearly *** this study,we performed in silico gene editing to exploit the AbaI gene,responsible for biofilm *** study explored different tools available for genome editing to create gene knockouts,selecting the *** AbaI gene as a target.

关键词： AbaI Acinetobacter baumannii CHOPCHOP Crispr-cas9 sgRNA

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Role of biosurfactants in bioremediation of oil pollution-a review

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Petroleum 2018年第3期4卷 241-249页

作者： Abraham Peele Karlapudi TCVenkateswarulu Jahnavi Tammineedi Lohit Kanumuri Bharath Kumar Ravuru Vijaya ramu Dirisala vidya prabhakar kodali Department of Biotechnology Vignan's Foundation for ScienceTechnology&ResearchVadlamudi522213India Department of Biotechnology Vikrama Simhapuri UniversityNellore524320India

The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the *** oil and oil products contamination may cause severe harm and hence,the attention has been remunerated in the development of alternative technologies for elimination of these *** were used in the remediation of oil pollution due to advantages such as biodegradability and low *** biosurfactants are produced from low cost substrates like agro-industrial wastes which reduce the cost of *** and bioemulsifiers are amphiphilic compounds and are produced as extracellular or a part of the cell membrane by *** insight view,how hydrocarbons are degraded by microorganisms and thereby reduce the damage of ecosystem is highly essential to target the *** are the bacterial communities which protects the bacterial cells from various adverse *** present review describes the biosurfactants and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation.

关键词： Biosurfactant Bioremediation Microbial enhanced oil recovery(MEOR) Biofilm

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Momordica charantia trypsin inhibitor Ⅱ inhibits growth and development of Helicoverpa armigera

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Insect Science 2009年第5期16卷 371-379页

作者： Manasi Alok Telang Prashant Pyati Mohini Sainani vidya Shrikant Gupta Ashok prabhakar Giri Plant Molecular Biology Unit Division of Biochemical Sciences National Chemical Laboratory Dr. Homi Bhabha Road Pune 411 008(M. S.) India

Bitter gourd （Momordica charantia L.） seeds contain several squash-type serine proteinase inhibitors （PIs）, which inhibit the digestive proteinases of the polyphagous insect pest Helicoverpa armigera. In the present work isolation of a DNA sequence encoding the mature peptide of a trypsin inhibitor McTI-Ⅱ, its cloning and expression as a recombinant protein using Pichia pastoris have been reported. Recombinant McTI-Ⅱ inhibited bovine trypsin at 1 ： 1 molar ratio, as expected, but did not inhibit chymotrypsin or elastase. McTI-Ⅱ also strongly inhibited trypsin-like proteinases （81% inhibition） as well as the total proteolytic activity of digestive proteinases （70% inhibition） from the midgut of H. armigera larvae. The insect larvae fed with McTI-Ⅱ-incorporated artificial diet suffered over 70% reduction in the average larval weight after 12 days of feeding. Moreover, ingestion of McTI-Ⅱ resulted in 23% mortality in the larval population. The strong antimetabolic activity of McTI-Ⅱ toward H. armigera indicates its probable use in developing insect tolerance in susceptible plants.

关键词： antimetabolic activity bitter gourd (Momordica charantia) digestive gut proteinases Helicoverpa armigera insect resistance squash family proteinase inhibitor

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