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Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

Phyton-International Journal of Experimental Botany 2022年第1期91卷 207-221页

作者： Olha Mykhailenko Ivan Bezruk Victoriia Volochai Volodymyr Mishchenko Liudas Ivanauskas victoriya georgiyants Department of Pharmaceutical Chemistry National University of PharmacyKharkiv61168Ukraine Department of Pharmacognosy National University of PharmacyKharkiv61168Ukraine Department of Quality Standardisation and Certification of DrugsNational University of PharmacyKharkiv61001Ukraine Department of Analytical and Toxicological Chemistry Lithuanian University of Health SciencesKaunas44307Lithuania

The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic *** of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus *** phytochemical screening of *** leaves revealed the presence of some following compound categories such as phenolic compounds,aminoacids,saponins,proteins,tannins,triterpenoids,glycosides,*** total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and *** activity was determined by ABTS radical-cation scavenging method,*** total amount of flavonoids in *** leaves was 1.07±0.02 mg RE/g(p<0.05),the total amount of phenolic compounds was 0.41±0.01 mg GAE/g(p<0.05).By HPLC-DAD-MS analysis the presence of the mangiferin,chlorogenic acid,isoorientin,kaempferol,hyperoside,and isoquercitin was established for the first time in Crocus *** antiradical activity of *** leaves extracts was 150.08±4.5μmol/g(p<0.05)and its was mainly attributed to phenolic compounds *** high amounts of flavonoids and antiradical activity in *** leaves suggests promising phytochemical and pharmacological study of this Crocus species.

关键词： Crocus speciosus flavonoids leaves antiradical activity

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