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Salmonella Typhi:from a Human Pathogen to a Vaccine Vector

Cellular & Molecular Immunology 2008年第2期5卷 91-97页

作者： victor tunje jeza Department of Immunology The State Key Laboratory of VirologyHubei Province Key Laboratory of Allergy and Immune-related DiseasesWuhan UniversitySchool of Medicine

Salmonella （S.） typhi is an important intracellular pathogen. Among the more than 2,300 closely-related Salmonella serovars bacteria recognized, S. typhi is the only one that is pathogenic exclusively for humans, in whom it causes typhoid or enteric fever. The pathogen has been around for many years and many studies have been done in an effort to combat it. Molecular and biologic features of S. typhi and host factors and immune responses involved in Salmonella invasion have been extensively studies. Vaccines that have been developed most notably are Vi polysaccharide and Ty21a. However, as the results show, there is still a long way to go. It is also shown that multi-drug resistance has occurred to the few available antibiotics. More and more studies have shown that Salmonella can be used as a vaccine vector carrying antigens of other pathogens. This has been promising in that the immune system can be elicited in response to both the Salmonella bacteria and the antigen of the pathogen in question. This review aims to highlight some of the milestones attained in the fight against the disease from the time S. typhi was seen as a pathogen causing typhoid fever to the use of Salmonella as a vaccine vector. Cellular ＆ Molecular Immunology.

关键词： Salmonella typhi vaccine vector typhoid Vi polysaccharide Ty21a

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L-ficolin对A型H1N1流感病毒感染小鼠的保护作用

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武汉大学学报（医学版） 2009年第6期30卷 701-704,709,F0002页

作者：潘勤侯炜 victor tunje jeza 章晓联武汉大学医学院免疫学系湖北武汉430071 武汉大学医学院病毒学国家重点实验室湖北武汉430071 武汉大学医学院病毒学研究所湖北武汉430071

目的:探讨L-ficolin分子与A型H1N1流感病毒的相互作用机制。方法:将pcDNA3.1-L-ficolin真核表达质粒以及对照质粒分别肌注小鼠后感染A型H1N1流感病毒,观察感染小鼠的体重变化、肺内病毒血凝滴度,并通过肺指数以及肺组织HE染色判断感染... 详细信息

目的:探讨L-ficolin分子与A型H1N1流感病毒的相互作用机制。方法:将pcDNA3.1-L-ficolin真核表达质粒以及对照质粒分别肌注小鼠后感染A型H1N1流感病毒,观察感染小鼠的体重变化、肺内病毒血凝滴度,并通过肺指数以及肺组织HE染色判断感染小鼠的肺部病变情况,ELISA检测肺组织IFN-γ和IL-4的水平。结果:A型H1N1流感病毒感染后,与对照组相比,注射了pcDNA3.1-L-ficolin质粒的小鼠体重无明显下降,肺内病毒血凝滴度较低,肺组织病变较轻,并且肺内IFN-γ和IL-4的水平增高。结论:pcDNA3.1-L-ficolin对小鼠抵御A型H1N1流感病毒感染有一定的保护作用,该质粒可作为抗流感病毒的新型候选制剂。

关键词：纤维胶凝蛋白 A型H1N1流感病毒感染

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Construction, Cloning, and Expression of HLA-A2-IgG2bFc Dimer for Donor-Specific Tolerance Induction

Construction, Cloning, and Expression of HLA-A2-IgG2bFc Dime...

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第八届全国免疫学学术大会

作者： victor tunje jeza 欧阳礼辰廖凡路陈俊单士刚翁秀芳吴雄文华中科技大学同济医学院免疫学系

Organ transplantation offers opportunity to a variety of patients with terminal functioning organs and ***, rejection of transplants has proved to be a formidable *** complete tolerance to allografts and xenografts without general immunosuppression is currently the holy grail of every transplantation *** immune suppressive drugs used for maintaining tolerance to allografts and xenografts has a major drawback of rendering the patient susceptible to infections and other side effects like malignancy and drug related toxicities with an overall rejection of the organ at some *** has been shown that MHCIg dimers can induce suppression of alloresponsive T cells in a donor specific ***, we set out to answer the question as whether these dimers will surmount rejection in *** do this, we began by constructing, cloning, and expressing HLA-A2-Ig G2 b Fc *** this purpose, we employed two *** was the construction from parts of an already cloned plasmid followed by overlap-PCR to form the full length HLA-A2 IgG2bFc insert which was then cloned to pc DNA3.1 to form pc DNA3.1-HLA-A2 *** second method was by extraction of mRNA from HLA-A2 transgenic mice to make the HLA-A2 c *** was then cloned into the pc DNA3.1 plasmid to form pc *** Ig G2 b Fc region was added by restriction digestion and ligation to form a clone as the first *** pc DNA3.1HLA-A2 IgG2bFc was then transfected by electroporation to J558 L *** was done using G418 antibiotic for four ***, ELISA was performed which confirmed that the transfection was *** dimer will be useful in that we will employ it in a bid to block rejection in a donor-specific fashion in congenic *** our model, the donor mice are transgenic for HLA-A2 while the recipient mice are transgenic for *** work is key to answer the aforementioned question on rejection.

关键词： HLA-A2 dimer Suppression Rejection and Donor-specific Alloresponse

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