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中国科学院地质与地球物理研究所2015年度（第15届）学术年会

作者： Jinhua li Nicolas Menguy Christophe Gatel victor Boureau Etienne Snoeck Gilles Patriarche Eric Leroy Yongxin Pan Paleomagnetism and Geochronology Lab Key Laboratory of Earth and Planetary PhysicsInstitute of Geology and Geophysics Chinese Academy of Sciences Institut de Mineralogie de Physique des Materiaux et de Cosmochimie (IMPMC) Sorbonne Universites—UPMC Univ Paris 06 UMR CNRS 7590 Museum National d’Histoire Naturelle (MNHN) IRD UMR 2064 Place Jussieu Paris 75005 France France-China Biomineralization and Nano-structures Laboratory Chinese Academy of Sciences CEMES/CNRS LPN-UPR20/CNRS Route de Nozay Marcoussis 91460 France

Magnetotactic bacteria(MTB) are known to produce single-domain magnetite or greigite crystals within intracellular membrane organelles and to navigate along the Earth’s magnetic field lines. MTB have been suggested as being one of the most ancient biomineralizing metabolisms on the Earth and they represent a fundamental model of intracellular biomineralization. Moreover, the determination of their specific crystallographic signature(e.g. structure and morphology) is essential for palaeoenvironmental and ancient-life studies. Yet, the mechanisms of MTB biomineralization remain poorly understood, although this process has been extensively studied in several cultured MTB strains in the Proteobacteria phylum. Here, we show a comprehensive transmission electron microscopy(TEM) study of magnetic and structural properties down to atomic scales on bullet-shaped magnetites produced by the uncultured strain MYR-1 belonging to the Nitrospirae phylum, a deeply branching phylogenetic MTB group. We observed a multiple-step crystal growth of MYR-1 magnetite: initial isotropic growth forming cubo-octahedral particles(less than approx. 40 nm), subsequent anisotropic growth and a systematic final elongation along [001] *** the crystal growth, one major f111 g face is well developed and preserved at the larger basal end of the crystal. The basal f111 g face appears to be terminated by a tetrahedral – octahedral-mixed iron surface, suggesting dimensional advantages for binding protein(s), which may template the crystallization of magnetite. This study offers new insights for understanding magnetite biomineralization within the Nitrospirae phylum.

关键词： magnetotactic bacteria Nitrospirae phylum bullet-shaped magnetite crystal growth biomineralization micromagnetic property

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Water budgets in an arid and alpine permafrost basin:Observations from the High Mountain Asia

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Advances in Climate Change Research 2024年第5期15卷 830-844页

作者： Qing-Feng WANG Hui-Jun JIN Dong-liang LUO Yu SHENG Jun WEN Chien-Lu PING Mark H.CLARK Qiang MA Xiao-Ying JIN Ji-Chun WU Yu-Zhong YANG Qiang li Raul D.SERBAN Daqing YANG victor F.BENSE Qing-Bai WU Key Laboratory of Cryospheric Science and Frozen Soil Engineering Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China Qinghai-Beiluhe Plateau Frozen Soil Engineering Safety National Oseration and Research Station Norhwes Institute of Eco-Environment and Resources Chinese Academy of Sciences Lanzhou 730000China School of Civil Engineering and Transportation China-Russia Joint Laboratory of Cold Regions Engineering and Environmentand Permafrost InstituteNortheast Forestry UniversityHarbin 150040 China College of Atmospheric Science Plateau Atmosphere and Environment Key Laboratory of Sichuan ProvinceChengdu University of Information Technology Chengdu 610225China Agricultural and Forestry Experiment Station University of Alaska FairbanksPalmer Research CenterPalmer 99645USA USDA-Natural Resources Conservation Service Palmer 99645USA College of Water Conservancy and Hydropower Engineering Gansu Agricultural UniversityLanzhou 730070China Institute for Alpine Environment Eurac ResearchBolzano 39100Ilialy Faculty of Agricultural Environmental and Food SciencesFree University of Bozen-BolzanoBolzano 39100Italy Watershed Hydrology and Ecology Division Environment and Climate Change CanadaVictoria V8S 3V3Canada Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China Hydrology and Quantiative Water Management Group Department of Environmental SciencesWageningen UniversityWageningen 6700The Netherlands

Ground freeze-thaw processes have significant impacts on infiltration,runoff and ***,there are still critical knowledge gaps in understanding of hydrological processes in permafrost regions,especially of the interactions among permafrost,ecology,and *** this study,an alpine permafrost basin on the northeastern Qinghai-Tibet Plateau was selected to conduct hydrological and meteorological *** analyzed the annual variations in runoff,precipitation,evapotranspiration,and changes in water storage,as well as the mechanisms for runoff gen-eration in the basin from May 2014 to December *** annual flow curve in the basin exhibited peaks both in spring and autumn *** high ratio of evapotranspiration to annual precipitation(>1.O)in the investigated wetland is mainly due to the considerably underestimated‘observed'precipitation caused by the wind-induced instrumental error and the neglect of snow *** stream flow from early May to late October probably came from the lateral discharge of subsurface flow in alpine *** study can provide data support and validation for hydrological model simulation and prediction,as well as water resource assessment,in the upper Yellow River Basin,especially for the headwater *** results also provide case support for permafrost hydrology modeling in ungauged or poorly gauged watersheds in the High Mountain Asia.

关键词： Water budget Runoff Freeze-thaw processes Active layer Permafrost basin Alpine wetland

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A multiple-tissue-specific magnetic resonance imaging model for diagnosing Parkinson’s disease: a brain radiomics study

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Neural Regeneration Research 2022年第12期17卷 2743-2749页

作者： Xiao-Jun Guan Tao Guo Cheng Zhou Ting Gao Jing-Jing Wu victor Han Steven Cao Hong-Jiang Wei Yu-Yao Zhang Min Xuan Quan-Quan Gu Pei-Yu Huang Chun-Lei liu Jia-li Pu Bao-Rong Zhang Feng Cui Xiao-Jun Xu Min-Ming Zhang Department of Radiology the Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Department of Neurology the Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Department of Electrical Engineering and Computer Sciences University of CaliforniaBerkeleyCAUSA Institute for Medical Imaging Technology School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghaiChina School of Information Science and Technology ShanghaiTech UniversityShanghaiChina Helen Wills Neuroscience Institute University of CaliforniaBerkeleyCAUSA Department of Radiology Hangzhou Hospital of Traditional Chinese MedicineHangzhouZhejiang ProvinceChina

Brain radiomics can reflect the characteristics of brain ***,the value of T1-weighted images,quantitative susceptibility mapping,and R2*mapping in the diagnosis of Parkinson’s disease(PD)was underestimated in previous *** this prospective study to establish a model for PD diagnosis based on brain imaging information,we collected high-resolution T1-weighted images,R2*mapping,and quantitative susceptibility imaging data from 171 patients with PD and 179 healthy controls recruited from August 2014 to August *** to the inclusion time,123 PD patients and 121 healthy controls were assigned to train the diagnostic model,while the remaining 106 subjects were assigned to the external validation *** extracted 1408 radiomics features,and then used data-driven feature selection to identify informative features that were significant for discriminating patients with PD from normal controls on the training *** informative features so identified were then used to construct a diagnostic model for *** constructed model contained 36 informative radiomics features,mainly representing abnormal subcortical iron distribution(especially in the substantia nigra),structural disorganization(e.g.,in the inferior temporal,paracentral,precuneus,insula,and precentral gyri),and texture misalignment in the subcortical nuclei(e.g.,caudate,globus pallidus,and thalamus).The predictive accuracy of the established model was 81.1±8.0%in the training *** the external validation dataset,the established model showed predictive accuracy of 78.5±2.1%.In the tests of identifying early and drug-naïve PD patients from healthy controls,the accuracies of the model constructed on the same 36 informative features were 80.3±7.1%and 79.1±6.5%,respectively,while the accuracies were 80.4±6.3%and 82.9±5.8%for diagnosing middle-to-late PD and those receiving drug management,*** accuracies for predicting tremor-dominant and non-tremor-dominant PD w

关键词： diagnosis imaging biomarker iron magnetic resonance imaging neuroimaging Parkinson’s disease quantitative susceptibility mapping R2*mapping radiomics T1-weighted imaging

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7系铝合金焊接技术的研究现状及展望

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材料导报 2023年第13期37卷 196-204页

作者：陈轩李萌蘖卜恒勇左汉宁昆明理工大学材料科学与工程学院昆明650093

本文主要对7系列铝合金焊接技术的现状进行总结和展望。当前7系铝合金的主要焊接工艺有电弧焊接、搅拌摩擦焊接、激光焊接以及复合焊接。电弧焊接主要分为钨极气体保护(TIG)焊、熔化极惰性气体保护(MIG)焊和冷金属过渡(CMT)焊接技术。7... 详细信息

本文主要对7系列铝合金焊接技术的现状进行总结和展望。当前7系铝合金的主要焊接工艺有电弧焊接、搅拌摩擦焊接、激光焊接以及复合焊接。电弧焊接主要分为钨极气体保护(TIG)焊、熔化极惰性气体保护(MIG)焊和冷金属过渡(CMT)焊接技术。7系铝合金采用CMT焊接工艺得到的焊缝美观,性能好。焊丝选择上,为避免焊缝中合金元素的烧损及裂纹敏感区,通常选用5系的铝镁焊丝。搅拌摩擦焊接是当前工程生产中铝合金焊接时采用最多的焊接方法,但不适用于形状复杂的焊接接头。激光焊接近年来通过发展已经在工业上得到推广及应用。未来7系铝合金焊接的研究方向是通过复合焊接工艺、开发新型焊丝、双丝焊接等方法来扩大其在工程上的使用范围。

关键词：铝合金焊接电弧焊接搅拌摩擦焊接激光焊接复合焊接

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瞬时受体电位M8离子通道对冷刺激诱导气道上皮细胞炎症反应的影响

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中华结核和呼吸杂志 2011年第10期34卷 757-761页

作者：李敏超 Juliy M. Perelman victor P. Kolosov 周向东重庆医科大学附属第二医院呼吸内科 400010 俄罗斯医学科学院远东呼吸生理与病理中心

目的探讨瞬时受体电位M8离子通道（TRPM8）在冷刺激诱导气道上皮细胞产生炎性因子过程中发挥的作用及相关信号转导机制。方法用冷空气（18℃）刺激人气道上皮16HBE细胞，以TRPM8通道特异性拮抗剂BCTC、TRPM8shRNA及蛋白激酶C（PKC）特... 详细信息

目的探讨瞬时受体电位M8离子通道（TRPM8）在冷刺激诱导气道上皮细胞产生炎性因子过程中发挥的作用及相关信号转导机制。方法用冷空气（18℃）刺激人气道上皮16HBE细胞，以TRPM8通道特异性拮抗剂BCTC、TRPM8shRNA及蛋白激酶C（PKC）特异性抑制剂钙磷酸蛋白C为干预手段，将细胞分为对照组（37℃培养）、冷刺激组、冷刺激＋BCTC组、冷刺激＋转染TRPM8 shRNA组、冷刺激＋转染对照shRNA组、冷刺激＋钙磷酸蛋白C组。Western blot法检测TRPM8 shRNA转染对16HBE细胞合成TRPM8蛋白的干扰效率；钙离子成像技术测量前5组细胞内每10s间隔的相对Ca^2＋浓度动态变化；EliSA法检测各组细胞分泌的白细胞介素（IL）-6、IL-8、肿瘤坏死因子（TNF）-α蛋白含量；实时荧光定量PCR检测各组细胞中IL-6、IL．8、TNF-α mRNA表达水平。结果冷刺激组细胞内相对Ca^2＋浓度最高值为2．36±0．24，显著高于对照组的1．01±0．02（t=12．52，P〈0．01），冷刺激＋BCTC组、冷刺激＋转染TRPM8 shRNA组细胞内相对Ca^2＋浓度降为1．47±0．17和1．26±0．12，显著低于冷刺激组（t值分别为6．69、9．12，均P〈0．01）；冷刺激组的IL-6、IL-8、TNF-α的mRNA和蛋白含量分别为0．66±0．16、0．77±0．15、0．73±0．09、（92±13）ng／L、（125±22）ng／L、（88±12）ng／L，较对照组[0．37±0．08、0．32±0．07、0．48±0．10、（52±8）ng／L、（50±9）ng／L、（61±8）ng/L]显著升高（t值分别为3．20、5．36、3．36、5．24、6．26、3．74，均P〈0．05），冷刺激＋BCTC组[分别为0．42±0．09、0．52±0．13、0．52±0．12、（72±8）ng／L、（92±14）ng／L、（68±11）ng／L]、冷刺激＋转染TRPM8 shRNA组[分别为0．41±0．10、0．49±0．08、0．50±0．08、（60±12）ng／L、（89±14）ng／L、（68±11）ng/L]、冷刺激＋钙磷酸蛋白C组[分别为0．40±0．07、0．44±0．09、0．47±0．08、（69±9）ng／L、（86±15）ng／L、（61±10）ng／L]较冷刺激组显著降低（均P〈0．05）；冷刺激＋转染对照shRNA组[分别为0．61±0．10、0．69±0．11、0．64±0．13、（89±13）ng/L、（118±20）ng／L、（79±13）ng／L]与冷刺激组比较差异无统计学意义（t值分别为0．48、0．79、1．12、0．35、0．43、1．00，均P〉0．05）。结论冷空气可通过激活气道上皮细胞上的TRPM8离子通道而诱导Ca^2＋内流进而激活下游PKC信号通路，进而导致代表性炎性因子的表达及生成增多。

关键词：瞬时受体电位通道蛋白激酶C 白细胞介素6 白细胞介素8 肿瘤坏死因子-α

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电渗析浓缩高盐溶液:阴阳离子交换膜匹配性关系

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中国科学技术大学学报 2021年第2期51卷 103-110页

作者：颜海洋李为汪耀明吴亮 Nikonenko victor Pismenskaya Natalia 徐铜文中国科学技术大学化学与材料科学学院应用化学系安徽省功能膜材料与技术工程实验室安徽合肥230026 俄罗斯库班国立大学俄罗斯克拉斯诺达尔市350040

研究了阳离子交换膜和阴离子交换膜的匹配性对电渗析浓缩性能的影响,主要考察了电渗析过程中的盐通量、水通量、水盐通量比和电渗析浓缩液的浓度.实验结果表明,当阳离子交换膜具有合适的离子交换容量时,阳离子交换膜的水含量对电渗析的... 详细信息

研究了阳离子交换膜和阴离子交换膜的匹配性对电渗析浓缩性能的影响,主要考察了电渗析过程中的盐通量、水通量、水盐通量比和电渗析浓缩液的浓度.实验结果表明,当阳离子交换膜具有合适的离子交换容量时,阳离子交换膜的水含量对电渗析的浓度性能有着重要的影响;相比于离子交换容量和水含量,阴离子交换膜的离子渗透性对电渗析浓缩性能有着更加重要的影响.此外,当阳离子交换膜和阴离子交换膜均具有较高离子渗透性时,阳离子交换膜对电渗析浓缩的影响更为重要.

关键词：电渗析阳离子交换膜阴离子交换膜匹配性关系浓缩性能

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Research on Railway Engineering Experiment Teaching Principles Based on Virtual Simulation Experiment Teaching——Taking the Floating Slab Vibration Damping Track Virtual Experiment as an Example

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Journal of Educational Theory and Management 2021年第2期5卷 103-108页

作者： Zhihui Zhu Zhiping Zeng Jing liu lili liu Lei Xu Wei Chen Wei li Jianyang Luo Joseph Eleojo victor School of Civil Engineering Central South UniversityChangshaHunan410075China Experimental Teaching Center of Civil Engineering Virtual Simulation Central South UniversityChangshaHunan410075China Department of Civil Engineering Faculty of EngineeringAhmadu Bello UniversityZariaKaduna State800242Nigeria

Virtual simulation teaching is an addendum to the experimental teaching mode of railway engineering,and the two teaching methods complement each other and merge with each *** view of the current research,there is little discussion about the integration path of the two *** on the connotation and design of virtual simulation teaching,this research systematically expounds the integration of the real path and path optimization problems,and puts forward the railway engineering experimental teaching principles based on virtual simulation *** the basis of this research,a virtual simulation experiment platform for vibration mechanics and its application in the floating slab vibration damping track was developed to make full use of three-dimensional modeling,virtual reality,human-computer interaction and other technologies,which can realistically simulate the vibration law and vibration damping effect of the rail transit system,and in the hope that the virtual simulation teaching can be widely used in the experimental teaching mode of railway engineering in the future.

关键词： Virtual simulation Experimental teaching Railway engineering Teaching principles Floating slab vibration damping track

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Exacerbated VEGF up-regulation accompanies diabetes-aggravated hemorrhage in mice after experimental cerebral ischemia and delayed reperfusion

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Neural Regeneration Research 2022年第7期17卷 1566-1575页

作者： Angela Ka Wai Lai Tsz Chung Ng victor Ka Lok Hung Ka Cheung Tam Chi Wai Cheung Sookja Kim Chung Amy Cheuk Yin Lo Department of Ophthalmology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong Special Administration RegionChina Department of Anesthesiology Laboratory and Clinical Research Institute for PainThe University of Hong KongHong Kong Special Administration RegionChina Macao University of Science and Technology TaipaMacao Special Administration RegionChinaSchool of Biomedical SciencesThe State Key Laboratory of Pharmaceutical BiotechnologyThe University of Hong KongHong Kong Special Administration RegionChina

Reperfusion therapy is the preferred treatment for ischemic stroke,but is hindered by its short treatment window,especially in patients with diabetes whose reperfusion after prolonged ischemia is often accompanied by exacerbated *** mechanisms underlying exacerbated hemorrhage are not fully *** study aimed to identify this mechanism by inducing prolonged 2-hour transient intraluminal middle cerebral artery occlusion in diabetic Ins2Akita/+mice to mimic patients with diabetes undergoing delayed mechanical *** results showed that at as early as 2 hours after reperfusion,Ins2Akita/+mice exhibited rapid development of neurological deficits,increased infarct and hemorrhagic transformation,together with exacerbated down-regulation of tight-junction protein ZO-1 and upregulation of blood-brain barrier-disrupting matrix metallopeptidase 2 and matrix metallopeptidase 9 when compared with normoglycemic Ins2+/+*** indicated that diabetes led to the rapid compromise of vessel integrity immediately after reperfusion,and consequently earlier death and further aggravation of hemorrhagic transformation 22 hours after *** observation was associated with earlier and stronger up-regulation of pro-angiogenic vascular endothelial growth factor(VEGF)and its downstream phospho-Erk1/2 at 2 hours after reperfusion,which was suggestive of premature angiogenesis induced by early VEGF up-regulation,resulting in rapid vessel disintegration in diabetic *** reticulum stress-related pro-apoptotic C/EBP homologous protein was overexpressed in challenged Ins2Akita/+mice,which suggests that the exacerbated VEGF up-regulation may be caused by overwhelming endoplasmic reticulum stress under diabetic *** conclusion,the results mimicked complications in patients with diabetes undergoing delayed mechanical thrombectomy,and diabetes-induced accelerated VEGF up-regulation is likely to underlie exacerbated hemorrhagic transformat

关键词： blood-brain barrier brain injury diabetes mellitus hemorrhagic transformation infarct ischemia/reperfusion injury middle cerebral artery occlusion mouse model stroke vascular endothelial growth factor

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Metastatic Colorectal Cancer: Optimizing Treatment with Anti-EGFR Monoclonal Antibody

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Journal of Cancer Therapy 2012年第6期3卷 902-911页

作者： Ka-On Lam victor Ho-Fun Lee Department of Clinical Oncology Li Ka Shing Faculty of Medicine The University of Hong Kong Hong Kong China.

Colorectal cancer remains a major cause of morbidity and mortality worldwide despite substantial improvement in the standard of care. Although surgical resection in selected patients may potentially be curative, systemic therapy is the only choice of treatment for most patients with metastatic colorectal cancer. Anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibody has established its role in the systemic therapy of metastatic colorectal cancer through multiple well-designed clinical trials and yet, the optimal use of anti-EGFR monoclonal antibody is undefined. This article serves to review the available evidence for anti-EGFR monoclonal antibodies and to speculate optimal strategy for their uses.

关键词： Colorectal Cancer Epidermal Growth Factor Receptor Cetuximab Panitumumab Strategy

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内生多肽Elafin对气道黏蛋白5AC表达的影响

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中华结核和呼吸杂志 2010年第11期33卷 801-805页

作者：余红梅李琪 Juliy M.Perelman victor P.Kolosov 周向东重庆医科大学附属第二医院呼吸内科 400010 俄罗斯医学科学院远东呼吸生理与病理中心

目的探讨内生多肽Elafin调节气道黏液高分泌的分子机制.方法构建人 Elafin 重组质粒,原代培养正常人支气管上皮细胞 HBE16,分为对照组、香烟抽提物（CSE）刺激组、CSE 刺激＋转染重组质粒、CSE 刺激＋空质粒组、单纯转染重组质粒以及... 详细信息

目的探讨内生多肽Elafin调节气道黏液高分泌的分子机制.方法构建人 Elafin 重组质粒,原代培养正常人支气管上皮细胞 HBE16,分为对照组、香烟抽提物（CSE）刺激组、CSE 刺激＋转染重组质粒、CSE 刺激＋空质粒组、单纯转染重组质粒以及空质粒组6组.四甲基偶氮唑盐法检测各组细胞活力,Western blot法检测p-JNK、p-ERK、p-P38和IκBα蛋白含量,荧光素酶报告基因系统测定激活蛋白-1（AP-1）及核转录因子-κB（NF-κB）活性,RT-PCR检测各组黏蛋白（MUC）5AC mRNA 表达水平,EliSA 法分析各组细胞 MUC5AC 蛋白的相对含量.结果 CSE刺激组的p-JNK、p-ERK、p-c-Jun、IκBα、AP-1、NF-κB、MUC5AC和MUC5AC mRNA 含量分别为（0.55±0.03）μg/mg、（0.64±0.06）μg/mg、（0.60±0.07）μg/mg、（0.27±0.03）μg/mg、7.49±0.31、4.42±0.22、（0.71±0.04）mg/L和0.81±0.04,与对照组的（0.26±0.02）μg/mg、（0.30±0.05）μg/mg、（0.19±0.04）μg/mg（0.61±0.04）μg/mg、2.54±0.22、2.37±0.16、（0.23±0.02）mg/L和0.32±0.03比较差异有统计学意义（均P＜0.05）.转染重组Elafin后再给予CSE刺激,p-JNK、p-ERK、p-c-Jun、IκBα、AP-1、NF-κB、MUC5AC和MUC5AC mRNA含量分别为（0.38±0.04）μg/mg、（0.31±0.04）μg/mg、（0.14±0.03）μg/mg、（0.54±0.03）μg/mg、2.60±0.19、2.55±0.21、（0.28±0.03）mg/L、0.35±0.05,与CSE组相比差异有统计学意义（均P＜0.05）;p-P38蛋白含量在CSE刺激及转染Elafin前后无明显变化.结论内生多肽Elafin可降低JNK和ERK磷酸化水平并抑制IκBα蛋白降解,从而降低转录激活蛋白-1和核因子-κB的活性,下调黏蛋白5AC的高表达,而p38MAPK在其中的作用并不明显.

关键词：弹性蛋白酶抑制因子黏蛋白类吸烟转录因子AP-1 NF-κB

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