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RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops

Horticulture Research 2020年第1期7卷 527-549页

作者： Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic Vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo vicki mccracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn Vanderzande Thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace Michigan State University East LansingMI 48824USA Washington State University WenatcheeWA 98801USA University of Minnesota St.PaulMN 55108USA Clemson University ClemsonSC 29634USA University of Florida WimaumaFL 33598USA USDA-ARS CorvallisOR 97333USA Washington State University PuyallupWA 98371USA Washington State University PullmanWA 99164USA Cedar Lake Research Group PortlandOR 97215USA University Queensland BrisbaneQLDAustralia Wageningen University and Research 6700 AA WageningenThe Netherlands University of New Hampshire DurhamNH 03824USA

The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the *** 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural ***,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in *** RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research *** was designed to enable the routine application of modern genomics and genetics technologies in *** crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural *** review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future *** technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic *** of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection *** chasm is now bridged,accelerating rosaceous crop genetic improvement.

关键词： breeding cultivar crops

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马克斯·布朗利:YouTube的网红测评员

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空中英语教室（高级版．彭蒙惠英语） 2024年第6期 30-31,48,49页

作者： Harry mccracken 不详

马克斯·布朗利把我带进他的机器人房间,在那里我与“巨人”面对面。那是一个物如其名、价值25万美元的机械手臂,它气势磅,但又不失优雅,高高地算立在那位身高六尺三寸(约190厘米)的主人面前,他正带我参观位于新泽西州卡尼镇的工作室。

关键词：机械手臂机器人工作室布朗利 YouTube 新泽西州面对面

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太极拳腿部动作对老年人平衡相关功能影响的研究

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医用生物力学 2024年第S1期 580-581页

作者：毛敏 vicki S.Mercer 李甫中 Michael T.Gross Troy Blackburn 于冰美国北卡罗莱纳大学教堂山分校物理治疗系山东大学护理与康复学院美国俄勒冈研究中心美国北卡罗来纳大学教堂山分校运动科学系

目的尽管太极拳(TC)可以预防老年人跌倒,但其有效动作仍不清楚,这很大程度上限制了太极拳的临床应用。本研究旨在比较太极拳下肢运动(TC LEE)、8式太极拳(8式TC)和牵拉练习对改善老年人平衡相关功能的效果。方法本研究共招募了102名...

目的尽管太极拳(TC)可以预防老年人跌倒,但其有效动作仍不清楚,这很大程度上限制了太极拳的临床应用。本研究旨在比较太极拳下肢运动(TC LEE)、8式太极拳(8式TC)和牵拉练习对改善老年人平衡相关功能的效果。方法本研究共招募了102名符合纳排标准的受试者。所有受试者被随机分配到TC LEE组(n=40)、8式TC组(n=31)和牵拉组(n=31),并接受相应的干预。干预总时长16周,每周3次,每次1小时。本研究的评价指标包括Berg平衡量表(BBS)、起立与行走测试(TUG)、静态站立时中心压力(COP)数据、踝关节本体感觉和髋关节外展力矩。所有受试者在干预前和干预后分别进行了评价指标的收集。使用重复测量方差分析比较了3个组在干预前后的评价指标。使用非劣检验,比较了两种TC干预组在BBS和TUG测试上的变化。结果与牵拉组相比,两干预组干预后在所有评价指标上均有显著改善(P<0.002)。BBS变化量的95%置信区间(CI)上限(-0.8～1.3分)在等效边界(1.8分)内。而TUG变化量的95%CI上限(0.1～2.1 s)超出等效边界(0.7 s),TC LEE组TUG变化分数更大。结论TC LEE可以改善老年人平衡相关功能,并且在改善TUG方面比8式TC效果更好,说明腿部动作可能是太极拳的关键,可在临床应用上有所侧重。

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Efficient expression of multiple guide RNAs for CRISPR/Cas genome editing

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aBIOTECH 2020年第2期1卷 123-134页

作者： vicki Hsieh-Feng Yinong Yang Intercollege Graduate Degree Program in Plant Biology The Huck Institutes of the Life SciencesThe Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Plant Pathology and Environmental Microbiology The Pennsylvania State UniversityUniversity ParkPA 16802USA

The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein system(CRISPR/Cas)has recently become the most powerful tool available for genome engineering in various *** efficient and proper expression of multiple guide RNAs(gRNAs),the CRISPR/Cas system is particularly suitable for multiplex genome *** the past several years,different CRISPR/Cas expression strategies,such as two-component transcriptional unit,single transcriptional unit,and bidirectional promoter systems,have been developed to efficiently express gRNAs as well as Cas *** progress has been made to optimize gRNA production using different types of promoters and RNA processing strategies such as ribozymes,endogenous RNases,and exogenous endoribonuclease(Csy4).Besides being constitutively and ubiquitously expressed,inducible and spatiotemporal regulations of gRNA expression have been demonstrated using inducible,tissue-specific,and/or synthetic promoters for specific research *** recently,the emergence of CRISPR/Cas ribonucleoprotein delivery methods,such as engineered nanoparticles,further revolutionized transgene-free and multiplex genome *** this review,we discuss current strategies and future perspectives for efficient expression and engineering of gRNAs with a goal to facilitate CRISPR/Cas-based multiplex genome editing.

关键词： CRISPR/Cas Gene expression Genome engineering Guide RNA Multiplex editing

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Molecular phylogenetic analysis of Leibnitzia Cass.(Asteraceae:Mutisieae:Gerbera-complex),an Asian-North American disjunct genus

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Journal of Systematics and Evolution 2010年第3期48卷 161-174页

作者： Kristen E.BAIRD vicki A.FUNK Andrea WEEKS Department of Environmental Science and Policy George Mason University US National Herbarium Smithsonian InstitutionMRC 166P.O.Box 371012WashingtonDC 20013-7012USA

Leibnitzia comprises six species of perennial herbs that are adapted to high elevation conditions and is one of only two Asteraceae genera known to have an exclusively disjunct distribution spanning central to eastern Asia and North *** phylogenetic analysis of Leibnitzia and other Gerbera-complex members indicates that Leibnitzia is monophyletic,which is in contrast with our expectation that the American Leibnitzia species L. lyrata and *** would be more closely related to another American member of the Gerbera-complex,namely *** area reconstructions show that the historical biogeography of the Gerbera-complex mirrors that of the entire Asteraceae,with early diverging lineages located in South America that were followed by transfers to Africa and Eurasia and,most recently,to North *** transfer of Leibnitzia appears to have been directed from Asia to North *** calibrations of nuclear(ribosomal DNA internal transcribed spacer region)and chloroplast(trnL-rpl32 intron)DNA sequence data using relaxed clock methods and either mean rate or fossil-based priors unanimously support Miocene and younger divergence times for Gerbera-complex *** ages are not consistent with most Gondwanan vicariance episodes and,thus,the global distribution of Gerbera-complex members must be explained in large part by long-distance *** species of Leibnitzia are estimated to have diverged from their Asian ancestor during the Quaternary(ca.2 mya)and either migrated overland to North America via Beringia and retreated southwards along high elevation corridors to their present location in southwestern North America or were dispersed long distance.

关键词： Beringia biogeography Gerbera-complex Mutisieae.

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SYNERGISTIC EFFICACY OF ADENOVIRUS-MEDIATED BCL-XS GENE TRANSFER AND TOPOTECAN IN OVARIAN CANCER CELL

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Chinese Journal of Cancer Research 2001年第4期13卷 254-257页

作者：王和 vicki V Baker West China Second Hospital Sichuan University Chengdu 610041 China University of Michigan Comprehensive Cancer Center MI 48109-0724 USA

Objective: To observe the synergistic efficacy between Adenovirus-mediated bcl-Xs(Adv-bcl-Xs) gene transfer and chemotherapy on ovarian cancer cell growth. Methods: NuTu-19 cells were infected by different titers of Adv-bcl-Xs and treated with topotecan in the meantime. Cell proliferation was measured 3 days later by MTT. Graphical representations and statistical analyses for their interaction in tumor cells were done. Results: The statistical result and Graphical representations of the statistical modeling showed synergy effect on cell growth inhibition (P<0.01). Conclusion: There were synergistic efficacies between Adv-bcl-Xs gene therapy and Topotecan in ovarian cancer cell growth.

关键词： bcl-Xs Topotecan Ovarian Cancer

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Enhanced destabilization of mismatched DNA using gold nanoparticles offers specificity without compromising sensitivity for nucleic acid analyses

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Nano Research 2015年第12期8卷 3922-3933页

作者： Abootaleb Sedighi vicki Whitehall Paul C. H. Li Department of Chemistry Simon Fraser University Burnaby BC VSA 1S6 Canada QIMR Berghofer Medical Research Institute 300 Herston Rd Brisbane QLD 4006 Australia

Here, we report a method that uses gold nanoparticles （AuNPs） to enhance the specificity of DNA hybridization without reducing its detection sensitivity. The conventional stringent wash method utilizes high-temperatureflow-salt conditions to enhance the specificity of DNA hybridization-based assays. This method creates a destabilizing environment for base pairing that affects specific and nonspecific duplexes. Therefore, specificity is achieved at the expense of signal intensity or sensitivity. However, in the proposed wash method, AuNPs predominantly destabilize nonspecific duplexes, offering specificity without compromising sensitivity. This AuNP wash technique has proven to be effective in detecting single nucleotide polymorphisms （SNPs） in genomic samples even at room temperature in a CD-like NanoBioArray （CD-NBA） chip. This method is also robust with sequence variation and is compatible with multiplex DNA analyses on microarrays. Thus, the AuNP wash method could potentially be useful for improving the accuracy of DNA hybridization results.

关键词： DNA hybridization gold nanoparticles single nucleotidepolymorphism specificity CD-like NanoBioArray chip

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NanoHDA： A nanoparticle-assisted isothermal amplification technique for genotyping assays

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Nano Research 2017年第1期10卷 12-21页

作者： Abootaleb Sedighi Christopher Oberc vicki Whitehall Paul C. H. Li Department of Chemistry Simon Fraser University Burnaby British Columbia VSA 1S6 Canada QIMR Berghofer Medical Research Institute 300 Herston Rd Brisbane Queensland 4006 Australia Department of Chemical and Physical Sciences University of Toronto Mississauga Ontario LSL IC6 Canada

Isothermal methods, such as helicase-dependent amplification （HDA）, have an advantage over polymerase chain reaction for DNA amplification owing to their ease of operation. Here, we developed a new HDA method that is nanoparticle-assisted, termed nanoHDA. This method uses gold nanoparticles （AuNPs） to improve the sensitivity and specificity of the isothermal method. In HDA, the denaturation of DNA templates is mediated by helicases, but this method is limited by the low denaturation efficiency of helicases. In this report, AuNPs with preferential affinity for single-stranded DNA （ssDNA） were utilized to improve the denaturation efficiency of helicases. The same affinity property of nanoparticles can also enhance specificity by suppressing primer-dimer formation. This nanoHDA method was employed to genotype the KRAS gene in genomic DNA samples from colorectal cancer patients, as achieved by the hybridization of nanoHDA amplicons using the NanoBioArray chip.

关键词： gold nanoparticle isothermal DNAamplification helicase genotyping assay colorectal cancer single nucleotidepolymorphism KRAS

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Computer education for teachers : 4th ed.

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2002年

作者： Sharp vicki F.

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Collection development and management for 21st century library collections :

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2011年

作者： vicki L. Gregory.

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