检索结果-南通市图书馆

Proteomics for discovery of candidate colorectal cancer biomarkers

World Journal of Gastroenterology 2014年第14期20卷 3804-3824页

作者： Paula lvarez-Chaver Olalla Otero-Estévez María Páez de la Cadena Francisco J Rodríguez-Berrocal vicenta s martínez-zorzano Unidad de Proteómica Servicio de Determinación EstructuralProteómica y GenómicaCentro de Apoyo Científico y Tecnológico a la InvestigaciónUniversidad de VigoCampus As Lagoas-Marcosende área de Bioquímica y Biología Molecular Departamento de BioquímicaGenética e InmunologíaFacultad de BiologíaUniversidad de VigoCampus As Lagoas-Marcosende

Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and specificity to detect this disease at early *** it is well known that the pathogenesis of CRC is a progressive accumulation of mutations in multiple genes,much less is known at the proteome ***,in the last years many proteomic studies have been conducted to find new candidate protein biomarkers for diagnosis,prognosis and as therapeutic targets for this malignancy,as well as to elucidate the molecular mechanisms of colorectal *** important advantage of the proteomic approaches is the capacity to look for multiple differentially expressed proteins in a single *** review provides an overview of the recent reports describing the different proteomic tools used for the discovery of new protein markers for CRC such as two-dimensional electrophoresis methods,quantitative mass spectrometry-based techniques or protein ***,we will also focus on the diverse biological samples used for CRC biomarker discovery such as tissue,serum and faeces,besides cell lines and murine models,discussing their advantages and disadvantages,and summarize the most frequently identified candidate CRC markers.

关键词： Colorectal cancer serum Tissue Proteins Biomarkers Proteomics Protein identification Mass spectrometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-frequency lacustrine sequence stratigraphy of clastic lakes: lessons from ancient successions

引用

Journal of Palaeogeography 2024年第4期13卷 621-645页

作者： Carlos Zavala Hua-Qing Liu Xiang-Bo Li Valentin Trobbiani Yang Li Mariano Arcuri Agustin zorzano Departamento de Geología Universidad Nacional del Sur (UNS) GCs Consulting services.Bahía Blanca Research Institute of Petroleum Exploration&Development-Northwest Consejo Nacional de Investigaciones Científicas y T ecnicas (CONICET) shandong University of science and Technology

sequence stratigraphic concepts and methods provide novel tools for performing stratigraphic analysis, allowing us to improve our understanding of depositional models and basin evolution. Main controls and depositional elements(e.g., surfaces, systems tracts, parasequences, etc.) recognized in conventional sequence stratigraphy are designed for marine-related systems. In contrast, the sequence stratigraphy of lacustrine successions is much more complex and poorly understood, because it is not driven by sea-level changes, but by a complex interaction between tectonics and high-frequency climatic cycles. The comprehensive analysis of the water balance of lacustrine systems allows the recognition of three types of lake conditions: Underfilled, balanced-fill and overfilled lakes. Understanding the lake conditions in ancient successions is fundamental for unraveling lacustrine sequence stratigraphy, since these conditions effectively control water salinity, internal stacking pattern and the characteristics of systems tracts. Underfilled lakes are hydrologically closed lakes, and consequently, the lake-level can highly fluctuate, driven by high-frequency wet-dry climatic cycles. During wet periods, rivers supply water and sediments, resulting in fining-and thinning-upward elementary depositional sequences(EDs's) accumulated during the transgressive systems tract(TsT). In contrast, dry periods are characterized by a relative lake-level fall with the subaerial exposure of lake margin areas during the regressive systems tract(RsT). Lake water salinity can fluctuate from brackish to hypersaline. Balanced-fill lakes are partially closed lakes, and consequently, they have characteristics of both underfilled and overfilled lakes. During the TsT, the lake is in underfilled condition, and consequently, the introduction of water and sediment will accumulate a fining-upward interval until reaching the spill point during the maximum flooding. The RsT is accumulated under an overfille

关键词： Lacustrine sequence stratigraphy Underfilled lakes Balanced-fill lakes Overfilled lakes Forced transgression Forced regression

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Deltas:a new classification expanding Bates's concepts

引用

Journal of Palaeogeography 2021年第3期10卷 341-355页

作者： Carlos Zavala Mariano Arcuri Mariano Di Meglio Agustin zorzano German Otharan Ainara Irastorza Antonela Torresi Dpto.de Geologia Universidad Nacional del Sur(UNS)Bahia BlancaArgentina GCs Argentina sRL Molina Campos 1508000 Bahia BlancaArgentina Consejo Nacional de Investigaciones Científicas y Tecnicas (CONICET)Buenos AiresArgentina

Deltas constitute complex depositional systems formed when a land-derived gravity-flow(carrying water and sediments) discharges into a marine or lacustrine standing body of water. However, the complexity of deltaic sedimentary environments has been oversimplified by geoscientists over the years, considering just littoral deltas as the unique possible type of delta in natural systems. Nevertheless, a rational analysis suggests that deltas can be much more complex. In fact, the characteristics of deltaic deposits will depend on a complex interplay between the bulk density of the incoming flow and the salinity of the receiving water body. This paper explores the natural conditions of deltaic sedimentation according to different density contrasts. The rational analysis of deltaic systems allows to recognize three main fields for deltaic sedimentation, corresponding to(1) hypopycnal(2) homopycnal and(3) hyperpycnal delta settings. The hypopycnal delta field represents the situation when the bulk density of the incoming flow is lower than the density of the water in the basin. According to the salinity of the receiving water body, three different types of hypopycnal littoral deltas are recognized: hypersaline littoral deltas(HsLD), marine littoral deltas(MLD), and brackish littoral deltas(BLD). The basin salinity will determine the capacity of the delta for producing effective buoyant plumes, and consequently the characteristics and extension of prodelta *** littoral deltas(HOLD) form when the density of the incoming flow is roughly similar to the density of the water in the receiving basin. This situation is typical of clean bedload-dominated rivers entering freshwater lakes. Delta front deposits are dominated by sediment avalanches. Typical fallout prodelta deposits are absent or poorly developed since no buoyant plumes are generated. Hyperpycnal deltas form when the bulk density of the incoming flow is higher than the density of the water in the receivi

关键词： Deltas Hyperpycnal flows Homopycnal flows Hypopycnal flows Hyperpycnites Turbidites sediment gravity flows Gravity flows

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insulin-mimetic compound hexaquis(benzylammonium) decavanadate is antilipolytic in human fat cells

引用

World Journal of Diabetes 2017年第4期8卷 143-153页

作者： Christian Carpéné silvia Garcia-Vicente marta serrano Luc marti ChloéBelles Miriam Royo Jean Galitzky Antonio zorzano Xavier Testar Institut des Maladies Métaboliques et Cardiovasculaires I2MCINSERM U1048Institut.National de la Santé et de la Recherche Médicale &Université Paul Sabatier Genmedica Therapeutics s.L. Esplugues de Llobregat Combinatorial Chemistry Unit Barcelona Science ParkUniversity of Barcelona CIBER-BBN Networking Centre on BioengineeringBiomaterials and Nanomedicine Departament de Bioquimica i Biomedicina Molecular Facultat de BiologiaUniversitat de Barcelona CIBER de Diabetes y Enfermedades Metabólicas Asociadas(CIBERDEM) Instituto de Salud Carlos Ⅲ Institute for Research in Biomedicine(IRB Barcelona) the Barcelona Institute of Science and Technology

AIM To assess in rodent and human adipocytes the antilipolytic capacity of hexaquis(benzylammonium) decavanadate(B6V10), previously shown to exert antidiabetic effects in rodent models, such as lowering free fatty acids(FFA) and glucose circulating *** Adipose tissue(AT) samples were obtained after informed consent from overweight women undergoing plastic surgery. Comparison of the effects of B6V10 and reference antilipolytic agents(insulin,benzylamine,vanadate) on the lipolytic activity was performed on adipocytes freshly isolated from rat, mouse and human AT. Glycerol release was measured using colorimetric assay as an index of lipolytic activity. The influence of B6V10 and reference agents on glucose transport into human fat cells was determined using the radiolabelled 2-deoxyglucose uptake *** In all the species studied, B6V10 exhibited a dosedependent inhibition of adipocyte lipolysis when triglyceride breakdown was moderately enhanced by β-adrenergic receptor stimulation. B6V10 exerted on human adipocyte a maximal lipolysis inhibition of glycerol release that was stronger than that elicited by insulin. However, B6V10 did not inhibit basal and maximally stimulated lipolysis. When incubated at dose ≥ 10 μmol/L, B6V10 stimulated by twofold the glucose uptake in human fat cells, but-similarly to benzylamine-without reaching the maximal effect of insulin, while it reproduced one-half of the insulin-stimulation of lipogenesis in mouse fat cells. CONCLUsION B6V10 exerts insulin-like actions in adipocytes, including lipolysis inhibition and glucose transport activation. B6V10 may be useful in limiting lipotoxicity related to obesity and insulin resistance.

关键词： Adipocyte Lipolysis Amine oxidases Insulin resistance Obesity Hydrogen peroxide Vanadium Antidiabetics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential of soluble CD26 as a serum marker for colorectal cancer detection

引用

World Journal of Clinical Oncology 2011年第6期2卷 245-261页

作者： Oscar J Cordero Monica Imbernon Loretta De Chiara vicenta s martinez-zorzano Daniel Ayude Maria Paez de la Cadena F Javier Rodriguez-Berrocal Department of Biochemistry and Molecular Biology University of Santiago de CompostelaSchool of BiologyCIBUUS BuildingCampus Vida15782 Santiago de CompostelaSpain Department of Biochemistry Genetics and ImmunologyUniversity of VigoSchool of BiologyAs Lagoas-Marcosende s/n36310 VigoSpain

Colorectal cancer is characterized by a low survival rate even though the basis for colon cancer development,which involves the evolution of adenomas to carcinoma,is ***,the mortality rates continue to rise in economically transitioning countries although there is the opportunity to intervene in the natural history of the adenoma–cancer sequence through risk factors,screening,and *** in particular accounted for most of the decline in colorectal cancer mortality achieved in the UsA during the period *** show a better prognosis when the neoplasm is diagnosed *** the variety of screening strategies,the methods range from invasive and costly procedures such as colonoscopy to more low-cost and non-invasive tests such as the fecal occult blood test(guaiac and immunochemical).As a non-invasive biological serum marker would be of great benefit because of the performance of the test,several biomarkers,including cytologic assays,DNA and mRNA,and soluble proteins,have been *** found that the soluble CD26(sCD26)concentration is diminished in serum of colorectal cancer patients compared to healthy donors,suggesting the potential utility of a sCD26 immunochemical detection test for early ***26 originates from plasma membrane CD26 lacking its transmembrane and cytoplasmic *** 90%–95%of sCD26 has been associated with serum dipeptidyl peptidase IV(DPPIV)***-IV,assigned to the CD26 cluster,is a pleiotropic enzyme expressed mainly on epithelial cells and *** studies intended to validate this test for population screening to detect colorectal cancer and advanced adenomas are reviewed here.

关键词： Antibody arrays Bioinformatics Biomarkers cancer Clustering Cytometric beads Diagnosis Dipeptidyl peptidaseⅣ Enzyme linked immunosorbent assay Prognosis soluble CD26 screening serum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An Important Pool of sucrose Linked to starch Biosynthesis is Taken up by Endocytosis in Heterotrophic Cells

An Important Pool of Sucrose Linked to Starch Biosynthesis i...

引用

The second International symposium on Plant Neurobiology

作者： Edurne Baroja-Fernandez Ed Etxeberria Francisco José Muoz María Teresa Morán-zorzano Nora Alonso-Casajús Pedro Gonzalez Javier Pozueta-Romero Agrobioteknologia Instituta Nafarroako Unibertsitate Publikoa Gobierno de Navarra and Consejo Superior de Investigations CientificasMutiloako etorbidea zenbaki gabe31192 MutiloabetiNafarroaSpain University of Florida IFAs Citrus Research and Education Center700 Experiment Station RoadLake Alfred FL33850USA Agrobioteknologia Instituta Nafarroako Unibertsitate Publikoa Gobierno de Navarra and Consejo Superior de Investigations CientificasMutiloako etorbidea zenbaki gabe31192 MutiloabetiNafarroaSpain Agrobioteknologia Instituta Nafarroako Unibertsitate Publikoa Gobierno de Navarra and Consejo Superior de Investigations CientificasMutiloako etorbidea zenbaki gabe31192 MutiloabetiNafarroaSpain Agrobioteknologia Instituta Nafarroako Unibertsitate Publikoa Gobierno de Navarra and Consejo Superior de Investigations CientificasMutiloako etorbidea zenbaki gabe31192 MutiloabetiNafarroaSpain University of Florida IFAs Citrus Research and Education Center700 Experiment Station Road Lake Alfred FL33850USA Agrobioteknologia Instituta Nafarroako Unibertsitate Publikoa Gobierno de Navarra and Consejo Superior de Investigaciones CientificasMutiloako etorbidea zenbaki gabe31192 MutiloabetiNafarroaSpain

＜正＞We have recently shown the occurrence of endocytic sucrose uptake in heterotrophic cells （Etxeberria et al. Plant Cell Physiol. 46, 474-481）. Whether this mechanism is involved in the sucrose-starch conversion process was investigated by comparing the rates of starch accumulation in sycamore cells cultured in the presence or absence of the endocytic inhibitors wortmannin and LY294002. These analyses revealed a two-phase process involving an initial 120 min wortmannin- and

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

上皮结缔组织移植治疗牙龈退缩的组织学与临床研究:附1例报告

引用

中国口腔医学继续教育杂志 2018年第6期21卷 341-348页

作者： Ana-Maria Garcia-De-la-Fuente Luis Antonio Aguirre-zorzano Ruth Estefania-Fresco Laia Roig-Odena Jose-Manuel Aguirre-Urizar 张路(译) 李煌(审) 不详江苏省南京市口腔医院 210000

目的:上皮结缔组织移植(sCTG)被认为是治疗牙龈萎缩的金标准。出于伦理原因,大多数研究只报告临床结果.而不报告组织学结果。材料与方法:本研究报道一位20岁的女性,表现为下颌左中切牙局部牙龈退缩5毫米,她接受了sCTG治疗。结果:根据最... 详细信息

目的:上皮结缔组织移植(sCTG)被认为是治疗牙龈萎缩的金标准。出于伦理原因,大多数研究只报告临床结果.而不报告组织学结果。材料与方法:本研究报道一位20岁的女性,表现为下颌左中切牙局部牙龈退缩5毫米,她接受了sCTG治疗。结果:根据最初的治疗方案,在11个月后拔牙,并进行组织学研究,发现有胶原纤维的结缔组织新附着直接垂直插入牙本质区和新的牙骨质区(1.37mm)。

关键词：结缔组织移植移植治疗牙龈退缩组织学临床研究上皮牙龈萎缩临床结果

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

内皮葡萄糖载体对葡萄糖的识别和调节

引用

Oncology and Translational Medicine 1994年第1期11卷 30-30页

作者： P.Rsen A.Gross F.Vinals s.Zink M.Palacin A.zorzano 熊一力 Diabetes-Forschungsinstitut Düsseldorf University of BarcelonaBarcelona 同济医科大学附属同济医院

内皮摄取葡萄糖的调节和机制尚未阐明。因此我们研究了牛主动脉内皮细胞摄取葡萄糖,以识别各种葡萄糖载体,证明胰岛素和葡萄糖对葡萄糖载体表达的影响。葡萄糖摄取遵循 Michaelis-Menten动力学。内皮虽然存在高亲和力胰岛素受体,但胰岛... 详细信息

内皮摄取葡萄糖的调节和机制尚未阐明。因此我们研究了牛主动脉内皮细胞摄取葡萄糖,以识别各种葡萄糖载体,证明胰岛素和葡萄糖对葡萄糖载体表达的影响。葡萄糖摄取遵循 Michaelis-Menten动力学。内皮虽然存在高亲和力胰岛素受体,但胰岛素并不影响葡萄糖摄取。通过免疫标记,能鉴定出内皮细胞有 glut 1,但无 glut 4。增高葡萄糖浓度(10～44mM)预孵育牛主动脉内皮细胞,4小时内可减40%～50%的葡萄糖摄取,同时 glut 1蛋白含量减

关键词：葡萄糖胰岛素右旋糖己糖多肽内皮牛主动脉内皮细胞

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：