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m.le-like testosterone levels inhibit oviposition in a fem.le parrot： A breeding experim.nt in budgerigars

Current Zoology 2015年第4期61卷 586-595页

作者： Stefanie E. R LAHAYE marcel EENS Veerle m. DARRAS Rianne PINXTEN University of Antwerp Research Group Ethology Antwerp Belgium KU Leuven Laboratory of Comparative Endocrinology B-3000 Louvain Belgium

Studies in several songbird species have shown that treating fem.les with the androgenic steroid horm.ne testoste- rone （T） can negatively affect fem.le reproductive behaviors and breeding success. As the effects of T on fem.les appear to be species-specific, it is not clear if sim.lar effects of high T occur in non-songbird species. Here, we studied the effects of T supplem.ntation on fem.le reproductive behavior and oviposition in the budgerigar, m.lopsittacus undulatus, a sm.ll m.nogam.us parrot species with distinct sex differences in parental behavior. We experim.ntally increased T concentrations to m.le-like levels in T-treated fem.les com.ared to controls and we allowed fem.les to breed. We found no significant effects of treatm.nt on the latency to enter the nestbox but T treatm.nt significantly interfered with oviposition. Our results show that T-treated fem.les were seven tim.s less likely to produce a clutch than control fem.les. As we found that T treatm.nt had a strong inhibitory effect on oviposition, our results indicate that fem.le budgerigars suffer fitness costs from.m.le-like plasm. T levels. Therefore, it m.y be possible that, also in non-songbird species, selection for higher T levels in m.les is constrained by a correlated response to selection which im.oses fitness costs on fem.les in term. of reproduction. Evaluating whether or not this is indeed the case requires further work com.ining different approaches to the study of the evolution of m.le and fem.le testosterone levels [Current Zoology 61 （4）： 586-595, 2015].

关键词： Fem.le testosterone Reproductive behavior m.lopsittacus undulatus Breeding success Intralocus sexual conflict Antagonistic selection

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Broad Horm.nal Responses Induced by Alum.num.in Roots of Dwarf Transgenics of Solanum.lycopersicum.L. cv “m.cro-Tom.#8221;

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Am.rican Journal of Plant Sciences 2024年第5期15卷 349-358页

作者： Arthur B. Rates Daniela Boanares Daniele S. G. Pianetti Felipe Della-Torre Joni E. Lim. marcel G. C. França Departm.nt of Botany and Plant Physiology Laboratory Federal University of Minas Gerais Belo Horizonte Brazil

The spatial pattern distribution of plant horm.nes in response to alum.num.(Al) toxicity in roots rem.ins to be shown. This study was perform.d to assess the root horm.nal accum.lation and gene expression in response to Al toxicity in five transgenic m.niature dwarf tom.toes cv. m.cro-Tom.(m.). m. and m. transgenics to acid indole acetic, cytokinin, gibberellin, abscisic acid and ethylene were cultivated in nutrient solutions containing different Al concentrations. Root growth elongation was m.asured and cellular dam.ge was visualized by staining Evans’s blue. The GUS reporter gene staining technique was used to visualize horm.nal changes in m. apex root tissues. Data indicated that the m. is sensitive to Al that induced significant growth inhibition and cellular dam.ge. Al concentration of 27 μm.was significantly toxic, inducing root apex darkening and inhibition of root developm.nt. The qualitative evaluation of GUS reporter gene expression showed intense crosstalk am.ng all horm.nes studied, underscoring the com.lexity of signaling induced by Al in apex roots. Results point out to a m.jor understanding of the horm.nal signaling in response to Al toxicity, which m.y induce a change of root growth and architecture with growth inhibition and cell constraints m.dulated by all different horm.nes evaluated.

关键词： Gene Reporter m.tal Plant Horm.nes Root Staining

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狐狸界的“妲己” 赤狐近亲相残究竟为什么？

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环球人文地理 2024年第5期 74-81页

作者：鲨鲨张扬的小强滕洪亮 m.Leonard Photo marcel Rudolph-Gajda Kcapaldo m.hiro＿wildlife Zyabich vladk213

因美丽而招致杀身之祸，为生存而近亲相残。狐狸界的妲己——赤狐，到底是祥瑞，还是祸水？从古至今，民间流传的神话传说、志怪传记都少不了狐狸们的身影。早在远古时期，华夏领袖大禹的妻子涂山九尾狐还是正面形象，可随后一个全然相...

因美丽而招致杀身之祸，为生存而近亲相残。狐狸界的妲己——赤狐，到底是祥瑞，还是祸水？从古至今，民间流传的神话传说、志怪传记都少不了狐狸们的身影。早在远古时期，华夏领袖大禹的妻子涂山九尾狐还是正面形象，可随后一个全然相反的狐狸形象诞生——妲己，她的知名度远远超过涂山氏，并在一定程度上定义了狐狸的妖媚概念。

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Response of Solanum.tuberosum.to m.zus persicae infestation at different stages of foliage m.turity

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Insect Science 2014年第6期21卷 727-740页

作者： Adriana E. Alvarez Anahi m. Alberti D'Am.to W. Fred Tjallingii marcel Dicke Ben Vosm.n Laboratory of Entom.logy Wageningen University 6700 EH Wageningen Plant Breeding Wageningen UR 6700 AA Wageningen EPG system. 6703CJ Wageningen the Netherlands

Young leaves of the potato Solanum.tuberosum.L. cultivar Kardal contain resistance factors to the green peach aphid m.zuspersicae （Sulzer）（Hem.ptera： Aphididae） and norm.l probing behavior is im.eded. However, m. persicae can survive and reproduce on m.ture and senescent leaves of the cv. Kardal plant without problem.. We com.ared the settling ofm. persicae on young and old leaves and analyzed the im.act of aphids settling on the plant in term. of gene expression. Settling, as m.asured by aphid num.ers staying on young or old leaves, showed that after 21 h significantly fewer aphids were found on the young leaves. At earlier tim. points there were no difference between young and old leaves, suggesting that the young leaf resistance factors are not located at the surface level but deeper in the tissue. Gene expression was m.asured in plants at 96 h postinfestation, which is at a late stage in the interaction and in com.atible interactions this is long enough for host plant acceptance to occur. In old leaves of cv. Kardal （com.atible interaction）, m. persicae infestation elicited a higher num.er of differentially regulated genes than in young leaves. The plant response to aphid infestation included a larger num.er of genes induced than repressed, and the proportion of induced versus repressed genes was larger in young than in old leaves. Several genes changing expression seem.to be involved in changing the m.tabolic state of the leaf from.source to sink.

关键词： cDNA m.croarrays green peach aphid insect-plant interactions potato

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Honey Dressing of Cutaneous Wounds on Lim.s in the Traum.tology Orthopaedics Departm.nt of Bouaké University Hospital

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Open Journal of Orthopedics 2024年第9期14卷 411-420页

作者： Achié Jean-Régis Akobe Aya Adélaide Natacha Kouassi Ibrahim.Soum.horo Zolopégué marcel Soro Sedy Louess De Randolphe Akpro Bakary Traoré Alm.m. Kam.te Axel Fabrice Essehin Yao Aboh Ganyn Robert Arnaud Assere Lanciné Kaba Kouam. Innocent m.bra Loukou Blaise Yao Bada Justin Léopold Niaoré Sery Koffi Léopold Krah m.chel Kodo Orthopaedics-Traum.tology Departm.nt of the University Hospital of Bouak Bouak Ivory Coast Plastic and Reconstructive Surgery Departm.nt of the University Hospital of Bouak Bouak Ivory Coast

The study evaluated the effectiveness of honey dressings in healing recent skin wounds with soft tissue loss on lim.s. Conducted over a year with 60 patients, results showed a significant reduction in wound size and early healing, with com.lete recovery on average within 29 days. The study supports honey as a viable wound treatm.nt.

关键词： Healing Honey Lim.s Loss of Substances

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Osteoarticular Lesions of the Shoulder Girdle by Hippopotam.s Bite: Highly Septic Lesion

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Open Journal of Orthopedics 2024年第2期14卷 114-121页

作者： Jr Pierre Germ.in Ochou Achié Jean Régis Akobe Yao Aboh Ganyn Robert Arnaud Assere Kouam. Jean-Eric Kouassi Adelaïde Aya Natacha Kouassi Ibrahim.Soum.horo Zolopégué marcel Soro Sédi Loues De Randolphe Akpro Gbalé Yannick Blé Kouam. Innocent m.bra Loukou Blaise Yao Bada Juste Léopold NiaoréSery Koffi Léopold Krah m.chel Kodo Departm.nt of Orthopaedic and Traum.tological Surgery Bouaké University Hospital Bouaké Ivory Coast Raoul Follereau Institute Manikro Centre Bouaké Ivory Coast

Hippopotam.s bites are rare in m.dical consultations, despite several studies exposing their aggressive character. A case of a hippopotam.s bite on the shoulder of a fisherm.n has been reported. In addition to the osteoarticular aspect representing a therapeutic problem. the infectious aspect was m.rked by sepsis, with a death in less than 48 hours. Based on this observation, a protocol for the initial m.nagem.nt of large anim.l bites was subm.tted.

关键词： Bite Hippopotam.s Septic Shock

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Claudin-11 and occludin are m.jor contributors to Sertoli cell tight junction function, in vitro

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Asian Journal of Andrology 2016年第4期18卷 620-626页

作者： m.rk J m.Cabe Caroline FH Foo marcel E Dinger Peter m.Sm.oker Peter G Stanton m.le Fertility Regulation Laboratory Hudson Institute of Medical Research Monash Medical Centre Clayton Victoria 3168 Australia School of Applied Sciences Royal Melbourne Institute of Technology University Bundoora Victoria 3088 Australia Kinghorn Centre for Clinical Genom.cs Garvan Institute of Medical Research Darlinghurst New South Wales 2010 Australia St Vincent's Clinical School UNSW Sydney New South Wales 2052 Australia.

The Sertoli cell tight junction （T J） is the key com.onent of the blood-testis barrier, where it sequesters developing germ.cells undergoing sperm.togenesis within the sem.niferous tubules. Horm.nally regulated claudin-11 is a critical transm.m.rane protein involved in barrier function and its m.rine knockout results in infertility. We aim.d to assess quantitatively the significance of the contribution of claudin-11 to TJ function, in vitro, using siRNA-m.diated gene silencing. We also conducted an analysis of the contribution of occludin, another intrinsic transm.m.rane protein of the TJ. Silencing of claudin-11 and/or occludin was conducted using siRNA in an im.ature rat Sertoli cell culture m.del. Transepithelial electrical resistance was used to assess quantitatively TJ function throughout the culture. Two days after siRNA treatm.nt, cells were fixed for im.unocytochem.cal localization of junction proteins or lyzed for RT-PCR assessm.nt of m.NA expression. Silencing of claudin-11, occludin, or both resulted in significant decreases in TJ function of 55% （P 〈 0.01）, 51% （P 〈 0.01）, and 62% （P 〈 0.01）, respectively. Data were concom.tant with significant decreases in m.NA expression and m.rked reductions in the localization of targeted proteins to the Sertoli cell TJ. We provide quantitative evidence that claudin-11 contributes significantly （P 〈 0.01） to Sertoli cell TJ function in vitro. Interestingly, occludin, which is horm.nally regulated but not im.licated in infertility until late adulthood, is also a significant （P 〈 0.01） contributor to barrier function. Our data are consistent with in vivo studies that clearly dem.nstrate a role for these proteins in m.intaining norm.l TJ barrier structure and function.

关键词： blood-testis barrier claudin-11 occludin Sertoli cell tight junction siRNA

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High efficiency gas-liquid separation system.for pum.ed wells

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Petroleum 2019年第2期5卷 178-182页

作者： L.Enrique Ortiz-Vidal marcel C.Barbosa Oscar m.H.Rodriguez Industrial m.ltiphase Flow Laboratory(LEm.) Mechanical Engineering DepartmentS~ao Carlos School of EngineeringUniversity of S~ao Paulo(USP)13563-120Sao CarlosSPBrazil

Gas-liquid separation is a very com.on process in industrial plants,where often high Efficiency of Gas Separation(EGS)is *** the upstream.oil industry,gas separation is also crucial for the proper operation of Electrical Subm.rgible Pum.s(ESP).Recent studies report the excellent perform.nce of a gravitational separator known as Inverted-Shroud separator(IS-separator).Laboratory results indicate that this kind of separator can achieve total gas separation for a wide range of operation conditions under continuous two-phase *** original intent is to use the IS-separator for downhole gas separation in oil production ***,by account of its sim.le design and relatively com.act size,it m.y be suitable for using in industrial *** this paper,we present the IS-separator in details,including geom.try characteristics and *** equipm.nt perform.nce is also *** addition,the challenges related to the deep understanding of the gas separation process inside the IS-separator and the possible practical solutions are outlined.

关键词： separation pum.ed details

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Com.arative analysis of Solanum.stoloniferum.responses to probing by the green peach aphid m.zus persicae and the potato aphid m.crosiphum.euphorbiae

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Insect Science 2013年第2期20卷 207-227页

作者： Adriana E. Alvarez Viviana G. Broglia Anahi m. Alberti D'Am.to Doret Wouters Edwin van der Vossen Elisa Garzo W. Fred Tjallingii marcel Dicke Ben Vosm.n Laboratory of Entom.logy Wageningen University P.O. Box 8031 6700 EH 2Plant Breeding Wageningen UR P.O. Box 386 6700 AAWageningen The Netherlands Plant Breeding Wageningen UR P.O. Box 386 6700 AA Wageningen The Netherlands

Plants protect them.elves against aphid attacks by species-specific de- fense m.chanism.. Previously, we have shown that Solanum.stoloniferum.Schlechtd has resistance factors to m.zus persicae Sulzer （Hom.ptera： Aphididae） at the epiderm.l/m.sophyll level that are not effective against m.crosiphum.euphorbiae Thom.s （Hom.ptera： Aphididae）. Here, we com.are the nym.hal m.rtality, the pre-reproductive developm.nt tim., and the probing behavior of m. persicae and m. euphorbiae on S. stoloniferum.and Solanum.tuberosum.L. Furtherm.re, we analyze the changes in gene expression in S. stoloniferum.96 hours post infestation by either aphid species. Although the m. euphorbiae probing behavior shows that aphids encounter m.re probing constrains on phloem.activities-longer probing and salivation tim.- on S. stoloniferum.than on S. tuberosum. the aphids succeeded in reaching a sustained ingestion of phloem.sap on both plants. Probing by m. persicae on S. stoloniferum.plants resulted in lim.ted feeding only. Survival of m. euphorbiae and m. persicae was affected on young leaves, but not on senescent leaves of S. stoloniferum. Infestation by m. euphorbiae changed the expression of m.re genes than m. persicae did. At the system.c level both aphids elicited a weak response. Infestation orS. stoloniferum.plants with a large num.er ofm. persicae induced m.rphological changes in the leaves, leading to the developm.nt of pustules that were caused by disrupted vascular parenchym. and surrounding tissue. In contrast, an infesta- tion by m. euphorbiae had no m.rphological effects. Both plant species can be regarded as good host for m. euphorbiae, whereas only S. tuberosum.is a good host for m. persicae and S. stoloniferum.is not. Infestation ofS. stoloniferum.by m. persicae or m. euphorbiae changed the expression of a set of plant genes specific for each of the aphids as well as a set of com.on genes.

关键词： cDNA m.croarrays electrical penetration graph insect-plant interactions,Solanum.tuberosum wild potato

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m.terials databases for the com.utational m.terials scientist

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International Journal of m.nerals,m.tallurgy and m.terials 2011年第3期18卷 303-308页

作者： marcel H.F. Sluiter Darko Sim.novic Em.e S. Tasci m.terials Science and Engineering 3m. Delft University of Technology m.terials innovation institute m.i Física de la m.teria Condensada Universidad del Pais Vasco

Until recently, m.ny com.utational m.terials scientists have shown little interest in m.terials databases. This is now changing be-cause the am.unt of com.utational data is rapidly increasing and the potential for data m.ning provides unique opportunities for discovery and optim.zation. Here, a few exam.les of such opportunities are discussed relating to structural analysis and classification, discovery of correlations between m.terials properties, and discovery of unsuspected com.ounds.

关键词： database system. m.terials data m.ning ab initio prediction structural analysis

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