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Assembly and Analysis of Changes in Transcriptomes of Dairy Cattle Rumen Epithelia during Lactation and Dry Periods

Agricultural Sciences 2018年第5期9卷 619-638页

作者： Cong-Jun Li Robert W. Li Ransom L. Baldwin vi Animal Genomics & Improvement Laboratory Beltsville Agricultural Research Center ARS USDA Beltsville MD USA Animal Parasitic Diseases Laboratory Beltsville Agricultural Research Center ARS USDA Beltsville MD USA

Lactation in dairy cattle is coupled with increased nutrient requirements for milk synthesis. Therefore, dairy cattle metabolism has to adapt to meet lactation-associated challenges and requires major functional adjustments of the rumen and whole digestive system. This report describes the use of next-generation sequencing technology for assembly and profiling of the transcriptome of cattle rumen epithelial tissues from cattle in both dry and lactation periods. Transcriptomics profiling and comparison revealed extensive changes in gene expression related to metabolism in rumen epithelial tissue due to the adaptation to lactation. Ruminal epithelial adaptation to the challenges of metabolism and high nutrient requirements during lactation is presumably the primary triggers for these alterations in gene expression. Principal component analysis (PCA) indicated that the gene expression profiles of the rumen epithelia from dry and lactating cattle fall into two very distinct clusters. Gene Ontology (go) enrichment analysis revealed that the most go terms were related to various metabolic processes in lactating cattle. The most significantly (false discovery rate (FDR) p-value go term in biological processes was “carbohydrate derivative metabolic process”, followed by “nucleoside metabolic process”. Up-stream regulators, such as PPARA (Peroxisome proliferator-activated receptor alpha) gene, and up-regulated genes of molecular transporters are the focal points of this report.

关键词： Dairy Cattle Lactation RNA-Sequencing Transcriptome Rumen Epithelium Gene Expression

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