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Comparative Transcriptomic Analysis of Salt Adaptation in Roots of Contrasting Medicago truncatula Genotypes

Molecular Plant 2012年第5期5卷 1068-1081页

作者： Ons Zahaf Sandrine Blanchet Axel de Zelicourt Benoit Alunni Julie Plet Carole Laffont Laura de Lorenzo Sandrine Imbeaud Jean-Laurent lchante Anouck Diet Mounawer Badri Ana Zabalza Esther M. Gonzalez Herve Delacroix veronique Gruber Florian Frugier Martin Crespi Institut des Sciences du Vegetal (ISV) CNRS 91198 Gif-sur-Yvette France Universite Paris Diderot Paris 7 75205 Paris Cedex 13 France Departamento de Microbiologia y Parasitologia Universidad de Sevilla 41012 Sevilla Spain Present address: Centro Nacional de Biotecnologia (CNB) CSIC 28049 Madrid Spain Centre de Genetique Moleculaire (CGM) CNRS Gif/Orsay DNA Microarray Platform (GODMAP) Universite Paris-Sud 11 91190 Gif-sur-Yvette France Present address: Genomique Fonctionnelle des tumeurs solides INSERM IUH Universit~ Paris Descartes 75010 Paris France Laboratory of Legumes Centre of Biotechnology of Borj Cedria BP 901 2050 Hammam-Lif Tunisia Departamento de Ciencias del Medio Natural Universidad P~blica de Navarra 31006 Pamplona Spain

Evolutionary diversity can be driven by the interaction of plants with different environments. Molecular bases involved in ecological adaptations to abiotic constraints can be explored using genomic tools. Legumes are major crops worldwide and soil salinity is a main stress affecting yield in these plants. We analyzed in the Medicago truncatula legume the root transcriptome of two genotypes having contrasting responses to salt stress： TN1.11, sampled in a salty Tunisian soil, and the reference Jemalong A17 genotype. TN1.11 plants show increased root growth under salt stress as well as a differential accumulation of sodium ions when compared to A17. Transcriptomic analysis revealed specific gene clusters preferentially regulated by salt in root apices of TN1.11, notably those related to the auxin pathway and to changes in histone variant isoforms. Many genes encoding transcription factors （TFs） were also differentially regulated between the two genotypes in response to salt. Among those selected for functional studies, overexpression in roots of the A17 ge- notype of the bHLH-type TF most differentially regulated between genotypes improved significantly root growth under salt stress. Despite the global complexity of the differential transcriptional responses, we propose that an increase in this bHLH TF expression may be linked to the adaptation of M. truncatula to saline soil environments.

关键词： root architecture abiotic stress legume transcriptome bHLH.

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关于地球章动模型及其与地内磁场的耦合

关于地球章动模型及其与地内磁场的耦合

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中国天文学会恒星分会2004年学术年会

作者：黄乘利 veronique Dehant 廖新浩 Oliver de Viron Tim Van Hoolst 中科院上海天文台 Royal Observatory of Belgium 中科院上海天文台 Royal Observatory of Belgium Royal Observatory of Belgium

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The Use of the PCR-Based Dot-Blot Hybridization Assay to Detect Resistance Markers to Rifampicin and Streptomycin in Mycobacterium tuberculosis Isolates from the SW Region of Cameroon

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Journal of Tuberculosis Research 2016年第2期4卷 72-79页

作者： Irene Ane-Anyangwe Wilfred Fon Mbacham Henry Dilonga Meriki Teyim Pride Theresa Nkuo-Akenji veronique Mbeng Penlap Leopold Djomkam Tietcheu Damian Nota Anong Akindeh Mbuh Nji Vincent P. K. Titanji Department of Biochemistry and Microbiology Faculty of Science University of Buea Buea Cameroon Laboratory for Public Health Research Biotechnologies Biotechnology Centre University of Yaoundé Yaoundé Cameroon Mezam Polyclinic Bamenda Cameroon

Drug sensitivity testing to establish resistance to TB drugs takes many months to arrive at. Public health physicians have difficulties with such an approach due to long wait periods and cannot use it to establish community wide prevalence as a way to understand where resistance may be emerging faster and to limit its spread. The objective of this study was to use the dot-blot hybridization technique in the detection of resistance to rifamycin (RIF) and streptomycin (SM) in South- Western Cameroon and to compare the technique with the routine culture and drug susceptibility testing for detecting resistance in a resource poor country, Cameroon. A hospital-based study was conducted at the Regional hospitals of Buea and Limbe and Tiko Central Clinic. Tuberculosis (TB) patients aged 15 to 50 (mean age: 30.50 ± 8.33 standard deviation) were recruited for the study between December 2006 and April 2007. Cultures from 59 patients were tested for rifampicin and streptomycin sensitivity by the modified proportion method and mutational analysis for rpoB codon 516 and rrs codon 513 was performed by the dot-blot hybridization technique. Of the 59 sputum samples collected (36 were males and 23 were females) came from Buea 19 (32.2%), Limbe 20 (33.9%) and Tiko 20 (33.9%) towns respectively. Amplification for the gene showed that there was (59) 100% amplification with primers used for rpoB genes and 43 (72.9%) amplification with primers used for the rrs gene. Mutational analysis demonstrated that resistance to RIF was common in females (52.1%) than males (41.7%) while 6% of the samples were indeterminate. 12 (20.3%) samples showed phenotypic and genotypic resistance to RIF compared to 34 samples (58.1%) for SM. Phenotypic resistance and genotypic susceptibility were found in 5 (8.5%) RIF and 3 (4.7%) SM compared to phenotypic susceptibility and genotypic resistance that were found in 2 (3.5%) RIF and 3(4.7%) SM. Double mutation on rpoB and rrs genes occurred in 8 (13.6%) DNA sample

关键词： PCR-Based DOT-Blot Analysis Rifamycin Streptomycin SW Region

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满大人作家画像:巴尔扎克1842年前后作品中的中国

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跨文化对话 2017年第1期 451-472页

作者： [法]白纯相许玉婷(译) 法国勒阿弗尔大学国际关系学院不详

本文将巴尔扎克于1842年为朋友、艺术家博尔热的画册所著同名书评集《中国与中国人》与《人间喜剧》加以对照,说明1842年前后巴尔扎克作品中“中国”一词的含义没有实质的变化,主要指“别处”、“精致”以及“财富”,由此进一步论证,巴... 详细信息

本文将巴尔扎克于1842年为朋友、艺术家博尔热的画册所著同名书评集《中国与中国人》与《人间喜剧》加以对照,说明1842年前后巴尔扎克作品中“中国”一词的含义没有实质的变化,主要指“别处”、“精致”以及“财富”,由此进一步论证,巴尔扎克作品中的中国不是博尔热所呈现的现实的、简化了的中国,而是他想象的中国,是他童年时从自己的父亲以及耶稣会士格鲁贤神父那里继承来的中国.中国是作家的投影,通过在作品中呈现中国,通过购买中国工艺品,作家在文学和生活中创造了自己的中国宫殿,从而让自己成为法国的满大人.

关键词：巴尔扎克中国别处精致财富满大人

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细胞自毁的古老起源

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环球科学 2024年第11期 60-65页

作者：罗尼卡·格林伍德陈鑫(翻译) 不详广州医科大学基础医学院

细胞如何演化出终结自己生命的过程?最近的研究表明,细胞凋亡最早出现在数十亿年前,起源于具有原始社会性的细菌。在细胞开始自我毁灭之前,我们很难判断它何时处于即将被毁的临界状态。它似乎只是在做日常的工作,转录基因,制造蛋白质,... 详细信息

细胞如何演化出终结自己生命的过程?最近的研究表明,细胞凋亡最早出现在数十亿年前,起源于具有原始社会性的细菌。在细胞开始自我毁灭之前,我们很难判断它何时处于即将被毁的临界状态。它似乎只是在做日常的工作,转录基因,制造蛋白质,名为线粒体的能量工厂细胞器也在尽职尽责地产生能量。但是,当一个线粒体接收到某种信号时,原本通常处于平静状态的线粒体蛋白质就会联合起来,形成一个“死亡机器”。它们会以令人惊诉的方式彻底切割细胞。短短几小时内,细胞所建立的一切都化为乌有,只剩下少量膜泡状结构。

关键词：自我毁灭细胞凋亡临界状态线粒体泡状结构细胞器尽职尽责蛋白质

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Efficacy and tolerability of chitin-glucan combined with simethicone(GASTRAP^(■)DIRECT)in irritable bowel syndrome:A prospective,open-label,multicenter study

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2024年第3期15卷 10-22页

作者： Nathalie Talbodec Pauline Le Roy Peggy Fournier Benoit Lesage Elodie Lepoutre François Castex Jean Michel Godchaux Lionel Vandeville Benjamin Bismuth Xavier Lesage Pauline Bayart Michael Genin Christel Rousseaux veronique Maquet Salvatore Modica Pierre Desreumaux Caroline Valibouze Department of Gastroenterology Hôpital privéLe BoisLille 59000France Department of Gastroenterology Hôpital privéde Villeneuve d’AscqVilleneuve d’Ascq 59650France Univ.Lille CHU LilleULR 2694–METRICSÉvaluation des Technologies de Santéet des Pratiques MédicalesLille 59000France Development of Intestinal Biotech 1 Avenue Oscar LambretLille 59045France KitoZyme SA Parc Industriel des hauts Sarts Zone 2Rue de MilmortHerstal 4040Belgium BiOkuris A Parc Industriel des hauts Sarts Zone 2Rue de MilmortHerstal 4040Belgium Department of Hepato-Gastroenterology Lille University HospitalLille 59000France U1286-INFINITE Institute for Translational Research in InflammationUniv.LilleInsermCHU LilleLille 59000France Department of Digestive Surgery and Transplantation Lille University HospitalLille 59037France

BACKGROUND Irritable bowel syndrome(IBS),defined according to the Rome IV diagnostic criteria,is a chronic functional gastrointestinal disorder characterized by recurrent abdominal pain related to altered bowel ***-line recommended treatments are limited to combining drugs targeting predominant symptoms,particularly pain(antispasmodics),constipation(laxatives),and diarrhea(loperamide),yielding only a limited therapeutic ***^(■)DIRECT is a class IIa medical formulation composed of a combination of chitin-glucan and simethicone indicated for the symptomatic treatment of gas-related gastrointestinal disorders by combining different mechanisms of *** To evaluate the efficacy,tolerability,and safety of 4-week GASTRAP^(■)DIRECT treatment in patients with *** In this prospective,multicenter,open-label trial,120 patients with IBS received three sticks of GASTRAP^(■)DIRECT(1.5 g/d of chitin-glucan and 0.75 mg/d of simethicone)per day for 4 *** primary endpoint was the responder rate,defined as the number of patients whose abdominal pain score decreased by≥30%from baseline to week(W)*** analysis was performed using the per-protocol *** symptoms,impact of global symptoms on daily life,change in stool consistency,and improvement in defecatory disorders were *** Overall,100 patients were *** W4,67%(95%CI:57-75)showed improvement in abdominal pain(score:5.8±2.4 vs 2.9±2.0,P<0.0001).Similar improvements were observed for bloating[8.0±1.7 vs 4.7±2.9,P<0.0001;60%(95%CI:50-70)responders],abdominal distension[7.2±2.1 vs 4.4±3.1,P<0.0001;53%(95%CI:43-63)responders],and impact of global symptoms on daily life[7.1±2.0 vs 4.6±2.9,P<0.0001;54%(95%CI:44-64)responders].Stool consistency improved in most patients(90%and 57%for patients with liquid and hard stools,respectively).Overall,42%of patients with defecatory disorders reported very much/considerable improvements by *** severe adverse event occurred,and tolerabil

关键词： Chitin-glucan Irritable bowel syndrome Abdominal pain Flatulence Defecatory disorders Stool consistency Natural non-pharmacological treatment

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塔林Koidula街住宅

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城市环境设计 2011年第Z1期 166-170页

作者： Markus Kaasik Andres Ojari Ilmar Valdur Merjer Muurisepp Atelier le Balto-Marc Pouzol veronique Faucheur 王夏璐 3+1建筑事务所天津大学建筑学院

建筑位于Kadriorg公园(Tallinn最重要的历史风貌区)附近。目前,这一历史街区正在迅速发展成为居住区。沿Koidula大街挤满了不同时期的居民楼,使这条街道结构混乱。

关键词：住宅历史风貌区 Koidula 历史街区塔林

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The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner

The unconventional resistance protein PTR recognizes the Mag...

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第二十届中国作物学会学术年会

作者： Gui Xiao Nutthalak Laksanavilat Stella Cesari Karine Lambou Ma?l Baudin Ahmad Jalilian Mary Jeanie Telebanco-Yanoria veronique Chalvon Isabelle Meusnier Elisabeth Fournier Didier Tharreau Bo Zhou Jun Wu Thomas Kroj State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center International Rice Research Institute PHIM Plant Health Institute Univ.Montpellier INRAE CIRADInstitut Agro IRD College of Agronomy Hunan Agricultural University

Blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating rice diseases. Disease resistance genes such as Pi-ta or Pi-ta2 are critical to protect rice production from blast. Published work reports that Pi-ta codes for a nucleotide-binding and leucine-rich repeat protein(NLR) that recognizes the fungal protease-like effector AVR-Pita by direct binding. However, this model was challenged by the recent discovery that Pi-ta2 resistance, which also relies on AVR-Pita detection, is conferred by the unconventional resistance gene Ptr, which codes for a membrane protein with a cytoplasmic armadillo repeat domain. Using NLR Pi-ta and Ptr RNAi knock-down and CRISPR/Cas9 knock-out mutant rice lines, we found that AVR-Pita recognition relies solely on Ptr and that the NLR Pi-ta has no role in it, indicating that it is not the Pi-ta resistance gene. Different alleles of Ptr confer different recognition specificities. The A allele of Ptr(PtrA) detects all natural sequence variants of the effector and confers Pi-ta2 resistance, while the B allele of Ptr(PtrB) recognizes a restricted set of AVR-Pita alleles and, thereby, confers Pi-ta resistance. Analysis of natural diversity in AVR-Pita and of mutant and transgenic strains identified one specific polymorphism in the effector sequence that controls escape from PtrB-mediated resistance. Taken together, our work establishes that the M. oryzae effector AVR-Pita is detected in an allele-specific manner by the unconventional rice resistance protein Ptr and that the NLR Pi-ta has no function in Pi-ta resistance and the recognition of AVR-Pita.

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杜邦公司提醒您注意:您是否已拥有合适的工作服?

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劳动保护 2001年第7期 47-47页

作者： veronique Tyvek~R化学保护工作服公司(Luxe m bourg)!区域经理 Sch mitt

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青春版《牡丹亭》白先勇的青春梦

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普洱 2012年第2期 107-107页

作者： veronique

Y说，很多年前读到《桃花扇》最后一出的时候，惊叹真是难得的好戏。及到后来，读罢全文，却大为怅然：原来也就只得最后一处是绝妙文字。我会心大笑：真正称得上华妙辞章、深婉清扬的戏曲，在我的心里始终还是只有《牡丹亭》。“回头... 详细信息

Y说，很多年前读到《桃花扇》最后一出的时候，惊叹真是难得的好戏。及到后来，读罢全文，却大为怅然：原来也就只得最后一处是绝妙文字。我会心大笑：真正称得上华妙辞章、深婉清扬的戏曲，在我的心里始终还是只有《牡丹亭》。“回头皆幻景，对面知是谁”一句振聋发聩，固然能震得人心魄颤动，却到底不如“原来姹紫嫣红开遍”一段能唱得荡气回肠、绕梁三日仍不绝其韵。更何况二八佳人因情而死、因情而生，情主至情，足叫人心荡神摇。

关键词：青春版《牡丹亭》青春梦白先勇《桃花扇》人心戏曲至情韵

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