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An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 μL of Rabbit Serum

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American Journal of Analytical Chemistry 2017年第9期8卷 553-563页

作者： veronika schmitt Andras Szeitz Tara L.Klassen Urs O.Hafeli Faculty of Pharmaceutical Sciences The University of British ColumbiaVancouverCanada

A highly sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the quantification of vancomycin (VAN) in low volumes of rabbit serum. For each analysis, 2 μL rabbit serum was precipitated with methanol that contained the internal standard teicoplanin (TEI). The supernatant was transferred into a 384 well-plate, diluted with water, covered with a pierceable silicone mat and 5 μL was analyzed in positive ionization mode. The UHPLC-MS/MS consisted of an Agilent 1290 Infinity UHPLC system connected to an AB Sciex QTrap®5500 hybrid linear ion-trap triple quadrupole mass spectrometer equipped with a Turbo Spray source. Chromatographic separation was achieved using a Waters Acquity UPLC BEH C18 (1.7 μm, 2.1 mm × 100 mm) column, a VanGuard (1.7 μm, 2.1 × 5 mm) guard column and a mobile phase of water and methanol both containing 5 mM ammonium acetate with 0.1% formic acid. VAN was quantified with multiple reaction monitoring using the transitions of m/z 725.5/144.2, and TEI was monitored at m/z 940.6/316.4. The accuracy, precision, linearity, range and lower limit of quantification (LLOQ) were determined. The accuracy was ≤9.93% and the precision was ≤10.6%. The range was established as 0.1 to 40 μg·mL-1. The LLOQ was 0.1 μg·mL-1 VAN requiring 2 μL of sample with an accuracy of -20.2% and precision of 8.39%. The method was applied successfully to determine the VAN concentrations in rabbit serum after the i.v. administration of VAN via implanted ear catheters.

关键词： Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Vancomycin Rabbit Serum Pharmacokinetics

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A force-compensated compliant MEMS-amplifier with electrostatic anti-springs

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Microsystems & Nanoengineering 2023年第3期9卷 235-244页

作者： Philip schmitt Martin Hoffmann Microsystems Technology Ruhr-Universität BochumUniversitätsstr.15044801 BochumGermany

In this paper,an electrostatic compliant mechanical amplifier intended for force-compensated displacement amplification in MEMS sensor applications is ***,mechanical transformers that enhance a small input displacement into a large output displacement generate large forces at the input of the *** microsystem proposed here allows for the reduction and compensation of the input stiffness of the amplifier and any mechanical components connected to it while providing a constant amplification ratio at the same *** amplifying mechanism features bidirectional electrostatic anti-springs enabling the control of the stiffness by applying a constant DC *** electrode design of the anti-springs and its influence on the force-displacement characteristic,the side instability and the maximal displacement are studied through analytical approaches and supported by FEA and by *** on the derived models,a compliant electromechanical amplifier is developed,featuring an amplification ratio of *** this amplifier the initial input stiffness of 422 N/m could be reduced to 6.8 N/m by applying a voltage of 100 *** an additional application,we show how the amplifier can be used as a mechanical force sensor with tuneable sensitivity,where the forces at the input are transformed into large output *** experiments,we show how the sensitivity can be adjusted and increased by a factor of 25 by applying a voltage at the anti-springs.

关键词： amplifier electrostatic spring

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Significance of intercellular communication for neurodegenerative diseases

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Neural Regeneration Research 2022年第5期17卷 1015-1017页

作者： Hilal Cihankaya Carsten Theiss veronika Matschke Ruhr University Bochum Medical FacultyInstitute of AnatomyDepartment of CytologyBochumGermany International Graduate School of Neuroscience(IGSN) Ruhr-University BochumBochumGermany

The nervous system is a highly complex organization composed of its basicunit,the neuronal cells,and specialized supporting cells,the glial *** main glial cells in the central nervous system(CNS)are astrocytes,microgl... 详细信息

关键词： cytes diseases degenerative

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Spatial modeling issues in future smart cities

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Geo-Spatial Information Science 2013年第1期16卷 7-12页

作者： Gerhard schmitt Department of Architecture and Future Cities Laboratory ETH ZürichZürichSwitzerlandand ETH CREATESingapore

It is our goal to make today’s and future cities smart,sustainable and *** order to achieve this,it is fundamental to understand how each city works,to formalize the knowledge gained and to apply it to a city model as the base for simulations that can generate future scenarios with a high level of *** nature of this model,which must cover design,qualitative and quantitative aspects,has changed over *** this study,we focus on the role of the spatial dimension and of geometry in a city *** from being a dominating generative force in ancient cities,spatial modeling has developed into an underlying description language for present and future cities to define functions and properties of the city in space and *** example of the stocks and flows model applied to the city depicts where and how spatial modeling influences the design,construction and performance of the future Smart City.

关键词： future cities spatial modeling stocks and flows city models reality-based 3D models

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Geometrical Deposits on Microstructured Surfaces

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Journal of Bionic Engineering 2020年第4期17卷 851-865页

作者： veronika Kubyshkina Daniel Orejon Coinneach Mackenzie Dover Khellil Sefiane University of Edinburgh School of EngineeringKings BuildingsMayfield RoadEdinburghEH93JLUK Oden Institute for Computational Engineering and Sciences The University of Texas at AustinTexas TX 78712USA Tianjin Key Lab of Refrigeration Technology Tianjin University of CommerceTianjin 300134China

Research into evaporating droplets on patterned surfaces has grown exponentially,since the capacity to control droplet morphology has proven to have significant technological utility in emerging areas of fundamental research and industrial ***,we incorporate two interest domains-complex wetting patterns of droplets on structured surfaces and the ubiquitous coffee-ring phenomenon of nanofluids containing dispersed aluminium oxide *** lay out the surface design criteria by quantifying the effect of pillar density and shape on the wetting footprint of droplets,yielding complex polygon droplet *** work is not constrained to pure liquids only,as we delve into the shape selection of particle-laden droplets of different *** visualise the deposition patterns through microscopy on surfaces exhibiting different features and further establish the ordering of particles on microscale surface *** a high nanofluid concentration,we observe intriguing self-assembly of particles into highly ordered intricate *** collective findings of this work have the potential to enhance many industrial technologies,particularly attractive for high performance optical and electrical devices.

关键词： geometrical wetting tuneable surface topography nanosuspensions deposition patterns particle assembly

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Oxidative stress: the lowest common denominator of multiple diseases

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Neural Regeneration Research 2019年第2期14卷 238-241页

作者： veronika Matschke Carsten Theiss Johann Matschke Institute of Anatomy Department of Cytology Ruhr University Bochum Institute of Cell Biology (Cancer Research) University of Duisburg-Essen University Hospital Essen

Oxygen is essential to the human life and life of all aerobic organisms. The complete oxidation of nutrients for the biological energy supply is one of the most important prerequisites for the formation of higher life forms. However, cells that benefit from oxidative respiration also suffer from reactive oxygen species because they adapted to oxygen as an energy source. Healthy cells balance the formation and elimination of reactive oxygen species thereby creating and keeping reactive oxygen species-homeostasis. When the concentration of free radicals exceeds a critical level and homeostasis is disturbed, oxidative stress occurs leading to damage of multiple cellular molecules and compartments. Therefore, oxidative stress plays an important role in the physiology and pathology of various diseases. Often, the antioxidant protection system becomes pathologically unbalanced in the genesis of several diseases, leading to functional losses of the organism, as in the case of amyotrophic lateral sclerosis, or cells develop metabolic mechanisms to use this system as protection against external influences, such as in the case of glioblastoma cells. Either way, understanding the underlying deregulated mechanisms of the oxidative protection system would allow the development of novel treatment strategies for various diseases. Thus, regardless of the direction in which the reactive oxygen species-homeostasis disequilibrate, the focus should be on the oxidative protection system.

关键词： neurodegenerative disease amyotrophic lateral sclerosis cancer glioblastoma reactive oxygen species metabolism antioxidant protection system

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Chronic active and atrophic gastritis as significant contributing factor to the development of gastric cystica profunda

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World Journal of Gastroenterology 2024年第17期30卷 2308-2310页

作者： veronika Papp Pál Miheller Department of Surgery Transplantation and GastroenterologySemmelweis UniversityBudapest 1082Hungary

Gastric cystica profunda(GCP)is an uncommon but underestimated gastric *** precancerous potential determines its *** addition to previous mucosa injury due to operations,biopsy or polypectomy,chronic active and atrophic gastritis may also lead to the development of *** carefully examining the stomach and taking biopsy samples from the susceptible regions,the stage of atrophy can be *** atrophic gastritis is a risk factor for cancer evolvement and it can also contribute to GCPs *** frequently occur close to early gastric cancers(EGCs)or EGC can arise from the cystic *** resection is an effective and minimally invasive treat-ment in GCP.

关键词： Gastric cystica profunda Chronic active gastritis Atrophic gastritis Operative Link for Gastritis Assessment staging Early gastric cancer Endoscopic resection

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Forest Disturbance Enhanced the Activity of Epedaphic Collembola in Windthrown Stands of the High Tatra Mountains

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Journal of Mountain Science 2014年第2期11卷 449-463页

作者： veronika URBANOVIOVá Dana MIKLISOVá Lubomír KOVá Institute of Biology and Ecology Faculty of Science P.J. Safárik UniversityMoyzesova 11 Kosice 04001 Slovakia Institute of Parasitology Slovak Academy of SciencesHlinkova 3 Kosice 04001 Slovakia

The study aimed to assess the response of ep- and hemiedaphic Collembola communities(activity, richness, community structure) to a disturbance, the subsequent management regime and to the season(summer, winter) in a High Tatra Mountains spruce forest destroyed by a *** and clear-cut resulted in an initial increase in the activity of Collembola inhabiting open areas,opportunistic or generalist species, while forest specialists diminished in numbers or *** results indicated that treatment with nonextracted fallen trees(NEX) provided a better chance for forest species to survive compared with their survival in open habitats of extracted(EXT) and wildfire(FIR) treatments. Great species' potential of NEX treatment was indicated by Chao2 estimator and activity/species rarefaction curves. Communities of NEX treatment were more similar to the reference(REF) treatment, documented by ordination and cluster analyses. Thus, leaving fallen timber after a windthrow to natural process of succession is suitable for survival of forest species and maintenance of diversity in forests restoration than timber *** structure in wildfire(FIR) stands was the most dissimilar to the other treatments. Most of the species trapped in this treatment belonged to hemiedaphic life forms, while the activity of larger epedaphic species diminished. In contrast, the highest number of trapped Collembola in EXT treatment was connected with the larger-bodied epigeic species with fast dispersal ability. The trapping period affected both the number of individuals and species richness;Collembola activity and species diversity in the individual treatments were lower in winter compared with the summer period. Several species increased activity during the winter period, namely Folsomia penicula, Friesea mirabilis, F. truncata, Hypogastrura socialis and Protaphorura aurantiaca.

关键词： Wildfire Windstorm Clear-cutting Collembola Spruce forest

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Decomposition of labile and recalcitrant coniferous litter fractions affected by temperature during the growing season

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Journal of Forestry Research 2020年第4期31卷 1115-1121页

作者： veronika Jílková Kristyna Dufková Tomás Cajthaml Na Sádkách 7CeskéBudějovice 37005Czech Republic Faculty of Science Masaryk UniversityKotlárská267/2Brno 61137Czech Republic Institute for Environmental Studies Charles University in PragueBenátská2Prague 12801Czech Republic Institute of Microbiology Czech Academy of SciencesVídeňská1083Prague 14220Czech Republic

Temperate coniferous forest soils are considered important sinks of soil organic carbon(C).Fresh C inputs may,however,affect soil microbial activity,leading to increased organic matter decomposition and carbon dioxide *** consists of labile and recalcitrant fractions which are thought to be utilized by distinct microbial communities and at different rates during the growing *** this study,we incubated the whole litter(LC+RC),the labile(LC)and the recalcitrant(RC)fractions with the coniferous soil at two temperatures representing spring/autumn(10℃)and summer(20℃)for one *** respiration and microbial community composition were regularly determined using phospholipid fatty acids as *** LC fraction greatly increased soil respiration at the beginning of the incubation period but this effect was rather *** effect of the RC fraction persisted longer and,together with the LC+RC fraction,respiration increased during the whole incubation *** of the RC fraction was more strongly affected by higher temperatures than decomposition of the more labile fractions(LC and LC+RC).However,when we consider the relative increase in soil respiration compared to the dH2 O treatment,respiration increased more at a lower temperature,suggesting that available C is more important for microbial metabolism at lower *** C was added only once in our study,no changes in microbial community composition were detected,possibly because the microbial community is adapted to relatively low amounts of additional C such as the amounts naturally found in litter.

关键词： Temperate forest Picea abies Soil respiration Hot water-extractable carbon PLFA (phospholipid fatty acids)

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Why nanodiamond carriers manage to overcome drug resistance in cancer

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Cancer Drug Resistance 2020年第4期3卷 854-866页

作者： veronika Benson Abbas Amini Institute of Microbiology Czech Academy of SciencesPrague 14220Czech Republic Department of Mechanical Engineering Australian College of KuwaitSafat 13015Kuwait Center for Infrastructure Engineering Western Sydney UniversityPenrithNSW 2751Australia

Nanodiamonds represent an attractive potential carrier for anticancer *** main advantages of nanodiamond particles with respect to medical applications are their high compatibility with non-cancerous cells,feasible surface decoration with therapeutic and cancer-cell targeting molecules,and their relatively low manufacturing ***,nanodiamond carriers significantly increase treatment efficacy of the loaded drug,so anticancer drugs execute more effectively at a lower ***,lower drug dose results in less extensive side *** carriers decorated with a targeting molecule accumulate primarily in the tumor tissue,and those nanodiamond particles impair efflux of the drug from cancer *** approaches considering nanodiamond carriers were already tested in vitro,as well as in ***,researchers focus particularly on the possible side effects of nanodiamond carriers applied systemically in *** behavior of nanodiamond carriers depends heavily on their surface coatings,so each therapeutic complex must be evaluated ***,it seems that site-specific application of nanodiamond carriers is a rather safe therapeutic approach,but intravenous application needs further *** benefits of nanodiamond carriers are remarkable and represent a potent approach to overcome the drug resistance of many cancers.

关键词： Nanodiamond drug carrier drug resistance cancer therapy nanoparticles

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