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Stem cells and tissue engineering: an alternative treatment for craniofacial congenital malformations and articular degenerative diseases

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Plastic and Aesthetic Research 2020年第6期7卷 24-37页

作者： cristina velasquillo Antonio Madrazo-Ibarra Claudia Gutiérrez-Gómez Marcia Rosario Pérez-Dosal Yaaziel Melgarejo-Ramírez Clemente Ibarra Biotecnología Instituto Nacional de Rehabilitación LGIICiudad de México 14389México Hospital General Manuel Gea González and Instituto Nacional de Rehabilitación LGII Ciudad de México 14080México Médico Adscrito al Servicio de Cirugía Plástica Instituto Nacional de PediatríaCiudad de México 04530México Dirección General Instituto Nacional de Rehabilitación LGIICiudad de México 14389México

The life quality of patients with craniofacial malformations is severely affected by the physical disabilities caused by the malformation itself,but also by being subjected to bullying,which leads to a series of relevant psychological and societal effects that have an economic impact on the health *** clefts,notably cleft lip(CL),cleft palate,and microtia,are the most common craniofacial birth defects in humans and represent a substantial burden,both personal and *** the other hand,osteoarthritis is a widespread degenerative disease that is becoming more common due to the extension of the human lifespan and to an increase in injuries in young people as a result of their *** in tissue engineering as a part of regenerative medicine offer new hope to patients that can benefit from new tissue engineering therapies based on the supportive action of tailored 3D biomaterials and the synergic action of stem cells that can be driven to the process of bone and cartilage *** review provides an update on recent considerations for stem cells and studies on the use of advanced biomaterials and cell therapies for the regeneration of craniofacial congenital malformations and articular degenerative diseases.

关键词： Mesenchymal stromal cells microtia cartilage cleft lip cleft palate

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Skin 3D Bioprinting. Applications in Cosmetology

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Journal of Cosmetics, Dermatological Sciences and Applications 2013年第1期3卷 85-89页

作者： cristina velasquillo Eduardo A. Galue Lourdes Rodriquez Clemente Ibarra L. Guillermo Ibarra-Ibarra Biotecnología Instituto Nacional de Rehabilitación Mexico City Mexico Wake Forest Institute for Regenerative Medicine Regenerative Medicine Winston Salem USA

Tissue engineering has been used for the treatment of several skin diseases and lesions;however, tissue engineering and regenerative medicine also have a huge potential in cosmetology. They include skin substitutes, cell therapy and wound-healing treatments based on biomaterial-based replacements and 3D systems. This review focuses on the bioprinting technology and how it can improve skin functions, restoring pigmentation or helping hair follicles to develop.

关键词： 3D Bioprinting Skin Bioprinting Scaffolds Human Skin Cells Cosmetology

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A Novel and Functional Artificial Bruch’s Membrane Made of Poly (ε-Caprolactone)/Gelatin for Retinal Pigment Epithelium Restoration

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Advances in Bioscience and Biotechnology 2024年第8期15卷 443-461页

作者： López Edith Silva-Bermudez Phaedra Gonzalez-Salinas Roberto velasquillo cristina Torres-Romero Abigail López-Colomé Ana María Pérez Armando Gina Prado-Prone Edgar Ramos-Martínez Iván Ramos-Martínez Quiroz-Mercado Hugo Hernández-Zimbrón Luis Fernando Instituto de Fisiologa Celular Universidad Nacional Autnoma de Mxico Ciudad de Mxico City Mxico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa Instituto Nacional de Rehabilitación Luis Guiller-mo Ibarra Ibarra Ciudad de México México Departamento de Investigacin Asociacin Para Evitar la Ceguera Ciudad de Mxico Mxico Subdireccin de Investigacin Tecnolgica Instituto Nacional de Rehabilitacin Luis Guillermo Ibarra Ibarra Ciudad de Mxico Mxico Departamento de Biologa Celular y Tisular Facultad de Medicina Universidad Nacional Autnoma de Mxico Ciudad de Mxico Mxico Escuela de Ciencias Universidad Autnoma Benito Jurez de Oaxaca Oaxaca Mxico Departamento de Medicina y Zootecnia de Cerdos Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional utnoma de Mxico Mxico Laboratorio de Investigacion Interdisciplinaria rea de Optometria Escuela Nacional de Estudios Superiores Unidad Len Universidad Nacional Autnoma de Mxico Len Guanajuato Mxico Laboratorio Nacional de ptica de la Visin-Centro de Investigaciones en ptica Len Guanajuato Mxico

Purpose: The primary aim of this paper is to design, create, and improve functional and artificial Bruch’s membranes (BM) using bioengineering techniques, which can be applied in the treatment of maculopathies by supporting the growth and maintenance of retinal pigment epithelium (RPE) cells, thereby potentially enabling subretinal implantation in patients. Methods: We fabricated by electrospinning ultrathin 3D nanofibrous membranes from Polycaprolactone (PCL), and different concentrations of gelatin (5%, 15% and 30%). ARPE-19 cells were seeded onto these artificial membranes. MTT assays were performed in order to evaluate ARPE-19 cell viability and cytotoxicity. IF assays were realized to observe the ARPE-19 cells onto each membrane. Ultrastructure of the modified Bruch’s membrane and ARPE-19 morphology after 25 days of culture were studied with transmission and scanning electron microscopy. To evaluate expression changes in markers of ARPE-19 (RPE65 and ZO-1) qRT-PCR assays were realized. Data from three independent experiments were pooled and expressed as the mean SD. A confidence level of P 0.05 was considered to be statistically significant. Results ARPE-19 cells grew on PCL/Gelatin membranes mainly in PCL/30% gelatin combination, which had not cytotoxic effect. RPE65/cytokeratin-18/ and actin-positive ARPE-19 cells formed a correctly orientated monolayer of polygonal cells with morphological polarity. The apical cell surfaces exhibited abundant protruding microfolds. Moreover, zones of polygonal border look as if ARPE-19 cells fused among, suggesting the presence of tight junctions. The expression of RPE65 and ZO-1 was unchanged. Conclusions: PCL/30% Gelatine membranes may imitate the natural BM to such extent that they support RPE-cells and exhibited RPE-like morphology. The engineering of a human RPE monolayer with these artificial BM, emulating the in vivo retina, arouse their potential subretinal implantation in patients with wet age-related macular degene

关键词： Artificial Bruch’s Membrane Electrospinning ARPE-19 Cells Age-Related Macular Degeneration

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