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Proteomic response of Phaeocystis globosa to nitrogen limitation

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journal of Oceanology and limnology 2024年第1期42卷 141-149页

作者： Haisu lIU Ruiwang WEI Qiangyong lEI lei CUI Songhui lÜ Key laboratory of Eutrophication and Red Tide Prevention Research Center for Harmful Algae and Marine BiologyJinan UniversityGuangzhou 510632China Southern Marine Science and Engineering Guangdong laboratory Zhuhai 519082China National Engineering laboratory of Electronic and Information Standardization Shenzhen 518057China

Phaeocystis globosa is an important unicellular eukaryotic alga that can also form *** can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur *** far,the ecophysiology of *** has been investigated by numerous ***,the proteomic response of *** to nitrogen depletion remains largely *** compared four protein preparation methods of *** for two-dimensional electrophoresis(2-DE)(Urea/Triton X-100 with trichloroacetic acid(TCA)/acetone precipitation;TCA/acetone precipitation;Radio Immuno Precipitation Assay(RIPA)with TCA/acetone precipitation;and Tris buffer).Results show that the combination of RIPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect,based on which the proteomic response to nitrogen depletion was studied using *** addition,we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold(Pld not be identified,which might be attributed to the lack of genomic sequences of *** nitrogen limitation,replication protein-like,RNA ligase,and sn-glycerol-3-phosphate dehydrogenase were reduced,which may decrease the DNA replication level and ATP production in *** *** increase of endonucleaseⅢand transcriptional regulator enzyme may affect the metabolic and antioxidant function of *** cells and induce cell *** findings provide a basis for further proteomic study of *** and the optimization of protein preparation methods of marine microalgae.

关键词： Phaeocystis globosa nitrogen limitation proteomic response two-dimensional electrophoresis

Fusion and one-neutron stripping process for ^(6)li+^(94)Zr system around the Coulomb Barrier

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Chinese Physics C 2024年第9期48卷 133-141页

作者： Xuedou Su Guangxin Zhang Shipeng Hu Peiwei Wen H.O.Soler Gaolong Zhang Boshuai Cai Cenxi Yuan j.l.Ferreira Zhenwei jiao Mingli Wang Xiaoyu Wang Zhen Huang Huanqiao Zhang Chunlei Zhang Xiaoguang Wu Yun Zheng Congbo li Tianxiao liand j.lubian School of Physics Beihang UniversityBeijing 100191China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China Institute for Advanced Study in Nuclear Energy&Safety Shenzhen UniversityShenzhen 518060China Shenzhen Key laboratory of Research and Manufacture of High Purity Germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China China Institute of Atomic Energy Beijing 102413China Instituto de Física Universidade Federal FluminenseNiterói 24210-340Rio de JaneiroBrazil State Key laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China The Key laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China

The complete and incomplete fusion cross section as well as one-neutron stripping process of ^(6)li+^(94)Zr system were measured at the energies around the Coulomb barrier by onlineγ-ray *** addition to a 30%suppression factor when compared with the measured total fusion process,the complete fusion cross section in ^(6)li+^(94)Zr system was observed to be significantly lower than those in the nearby ^(6)li+^(90,96)Zr *** new experimental result implies that the coupling with breakup channel in the ^(6)li-induced fusion processes can be affected by the inner structure of the target,which is still not clear in any available model *** the one-neutron stripping process,the direct production cross sections for each level in 95Zr were extracted and compared with the coupled reaction channel calculation,offering a unique opportunity to examine the single-particle nature of the produced excited *** the fact that an overall overestimation of the production cross section for 954-keV and1618-keV levels was observed in the comparison,further investigation is highly demanded in order to understand the full reaction mechanism for the one-neutron stripping process induced by ^(6)li.

关键词： weakly bound nuclei complete fusion incomplete fusion transfer reaction

COVID-19 vaccination produces exercise-responsive SARS-CoV-2 specific T-cells regardless of infection history

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journal of Sport and Health Science 2024年第1期13卷 99-107页

作者： Kyle A.Smith Tiffany M.Zúñiga Forrest l.Baker Helena Batatinha Charles R.Pedlar Shane C.Burgess Michael P.Gustafson Emmanuel Katsanis Richard j.Simpson School of Nutritional Sciences and Wellness The University of ArizonaTucsonAZ 85721USA Department of Pediatrics The University of ArizonaTucsonAZ 85724USA Faculty of Sport Allied Health and Performance ScienceSt.Mary's UniversityTwickenham TW14SXUK Institute of Sport Exercise and Health University College LondonLondon WC1E 7HUUK Department of Animal and Comparative Biomedical Sciences The University of ArizonaTucsonAZ 85721USA The University of Arizona Cancer Center The University of ArizonaTucsonAZ 85719USA laboratory Medicine and Pathology Mayo Clinic ArizonaPhoenixAZ 85054USA Department of Immunobiology The University of ArizonaTucsonAZ 85724USA Department of Medicine The University of ArizonaTucsonAZ 85724USA Department of Pathology The University of ArizonaTucsonAZ 85724USA

Background:The mobilization and redistribution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)specific T-cells and neutralizing antibodies(nAbs)during exercise is purported to increase immune surveillance and protect against severe coronavirus disease 2019(COVID-19).We sought to determine if COVID-19 vaccination would elicit exercise-responsive SARS-CoV-2 T-cells and transiently alter nAb ***:Eighteen healthy participants completed a 20-min bout of graded cycling exercise before and/or after receiving a COVID-19 *** major leukocyte subtypes were enumerated before,during,and after exercise by flow cytometry,and immune responses to SARS-CoV-2 were determined using whole blood peptide stimulation assays,T-cell receptor(TCR)-βsequencing,and SARS-CoV-2 nAb ***:COVID-19 vaccination had no effect on the mobilization or egress of major leukocyte subsets in response to intensity-controlled graded ***,non-infected participants had a significantly reduced mobilization of CD4+and CD8+naive T-cells,as well as CD4+central memory T-cells,after vaccination(synthetic immunity group);this was not seen after vaccination in those with prior SARS-CoV-2 infection(hybrid immunity group).Acute exercise after vaccination robustly mobilized SARS-CoV-2 specific T-cells to blood in an intensity-dependent *** groups mobilized T-cells that reacted to spike protein;however,only the hybrid immunity group mobilized T-cells that reacted to membrane and nucleocapsid *** increased significantly during exercise only in the hybrid immunity ***:These data indicate that acute exercise mobilizes SARS-CoV-2 specific T-cells that recognize spike protein and increases the redistribution of nAbs in individuals with hybrid immunity.

关键词： Anti-viral COVID-19 Exercise immunology SARS-CoV-2 T-Cells Vaccine

Ground-based and additional science support for SMIlE

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Earth and Planetary Physics 2024年第1期8卷 275-298页

作者： j.A.Carter M.Dunlop C.Forsyth K.Oksavik E.Donovon A.Kavanagh S.E.Milan T.Sergienko R.C.Fear D.G.Sibeck M.Connors T.Yeoman X.Tan M.G.G.T.Taylor K.McWilliams j.Gjerloev R.Barnes D.D.Billet G.Chisham A.Dimmock M.P.Freeman D.-S.Han M.D.Hartinger S.-Y.W.Hsieh Z.-j.Hu M.K.james l.j.usola K.Kauristie E.A.Kronberg M.lester j.Manuel.j.Matzka I.McCrea Y.Miyoshi j.Rae l.Ren F.Sigernes E.Spanswick K.Sterne A.Steuwer T.Sun M.-T.Walach B.Walsh C.Wang j.Weygand j.Wild j.Yan j.Zhang Q.-H.Zhang Planetary Sciences Group School of Physics and AstronomyUniversity of LeicesterUniversity RoadLeicesterLE17RHUK Rutherford Appleton laboratory Space Science Technology Facilities CouncilOxfordshireUK School of Space and Environment Beihang UniversityBeijing 100191China Key laboratory of Space Environment Monitoring and Information Processing Ministry of Industry and Information TechnologyBeijing 100017China Department of Space and Climate Physics UCLMullard Space Science LaboratoryHolmbury St.MaryDorkingSurreyRH56NTUK Birkeland Centre for Space Science Department of Physics and Technology.University of BergenBergenNorway Department of Physics and Astronomy University of Calgary2500 University DriveCalgaryAlbertaCanada T2N 1N4 British Antarctic Survey CambridgeUK Swedish Institute of Space Physics KirunaSweden West Highfield Campus University of SouthamptonUniversity RoadSO171BJUK Goddard Space Flight Center NASAGreenbeltMarylandUSA Athabasca University AthabascaCanada ESTEC European Space AgencyNetherlands University of Saskatchewan SaskatoonCanada The johns Hopkins University Applied Physics laboratory LaurelMarylandUSA Swedish Institude of Space Physics UppsalaSE 75121Sweden Tongji University Shanghai 200092China Center for Space Plasma Physics Space Science Institute4765 Walnut Street Suite BBoulderColorado80301USA Polar Research Institute of China Shanghai 200136China Finnish Meteorological Institute(FMI) Finland Department of Earth and Environmental Sciences(Geophysics) Ludwig Maximilian University of Munich(LMU)MunichTheresienstr.41MunichD-80333Germany Canadian Space Agency MontrealCanada GFZ German Research Centre for Geosciences PotsdamGermany Nagoya University Institute for Space Earth Environment ResearchCenter for Integrated Data ScienceNagoyaAichiJP 464-8601 Northumbria University Newcastle upon TyneTyne and WearUK NE18ST National Space Science Center Chinese Academy SciencesBeijing 100190China Arctic Geophysics University Centre in Svalbard

The joint European Space Agency and Chinese Academy of Sciences Solar wind Magnetosphere Ionosphere link Explorer(SMIlE)mission will explore global dynamics of the magnetosphere under varying solar wind and interplanetary magnetic field conditions,and simultaneously monitor the auroral response of the Northern Hemisphere *** these large-scale responses with medium and fine-scale measurements at a variety of cadences by additional ground-based and space-based instruments will enable a much greater scientific impact beyond the original goals of the SMIlE ***,we describe current community efforts to prepare for SMIlE,and the benefits and context various experiments that have explicitly expressed support for SMIlE can offer.A dedicated group of international scientists representing many different experiment types and geographical locations,the Ground-based and Additional Science Working Group,is facilitating these *** include constructing an online SMIlE Data Fusion Facility,the discussion of particular or special modes for experiments such as coherent and incoherent scatter radar,and the consideration of particular observing strategies and spacecraft *** anticipate growing interest and community engagement with the SMIlE mission,and we welcome novel ideas and insights from the solar-terrestrial community.

关键词： magnetosphere ionosphere magnetosphere-ionosphere coupling ground-based experimentation SMIlE conjunctions missions

Mid-term survival of the Optimys short stem: A prospective case series of 500 patients

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World journal of Orthopedics 2024年第3期15卷 257-265页

作者： Bryan Hamans Sheryl de Waard Sjoerd Kaarsemaker Esther R C janssen Inger N Sierevelt Gino M M j Kerkhoffs Daniël Haverkamp Department of Orthopaedic Surgery Xpert ClinicAmsterdam 1101EANetherlands Department of Orthopedic Surgery Amsterdam University Medical CenterAmsterdam 1105 AZNetherlands Esther R C janssen Department of Orthopaedic SurgeryViecuri Medisch CentrumVenlo 5912 BLNetherlands Centre for Orthopaedic Research Spaarne ZiekenhuisHoofddorp 2134 TMNetherlands Department of Orthopaedic Surgery and Sports Medicine Amsterdam UMC-Location AMCAmsterdam 1105 AZNetherlands Department of Orthopedic Surgery Xpert Orthopedic Surgery ClinicAmsterdam 1101 EANetherlands

BACKGROUND In recent years,there has been an increase in the number of total hip arthroplasty procedures in the younger patient *** active group has higher expectations of their prosthesis in comparison to the older population,and there is a greater physical demand for the *** femoral stems were in-troduced to retain proximal bone stock and joint biomechanics and became more common to implant in this specific ***,the long-term survival and functional outcomes of various short stems are still being investigated in different *** To determine the 5-year survival of the Optimys hip *** This was a prospective multicenter cohort study of 500 patients conducted in two hospitals in the *** patients received the Optimys short stem(Mathys ltd,Bettlach,Switzerland).The primary outcome measure was survival of the hip stem,with revision as the *** secondary outcome measurements included patient-reported outcome measures(PROMs).Kaplan-Meier analysis was used to calculate the 5-year survival ***-minus-log transformation was performed to calculate the 95%confidence interval(95%CI).Mixed model analyses were performed to assess the course of the PROMs during the 1st 2 years after *** were modeled separately for the 1st and 2nd years to calculate the yearly change in PROMs during both follow-up periods with accompanying 95%*** The mean age of the total 500 patients was 62.3 years(standard deviation:10.6)and 202 were male(40%).At a median follow-up of 5.5 years(interquartile range:4.5-6.7),7 patients were deceased and 6 revisions were registered,for infection(n=3),subsidence(n=2)and malposition(n=1).This resulted in an overall 5-year survival of 98.8%(95%CI:97.3-99.5).If infection was left out as reason for revision,a stem survival of 99.4%(95%CI:98.1-99.8)was *** questionnaires were completed by 471 patients(94%),317 patients(63%)completed the 1-year follow-up questionna

关键词： Total hip arthroplasty Femoral stem Short stem Optimys Survival 5-year survival Revision PROMs

Optimization of bio-oil production parameters from the pyrolysis of elephant grass (Pennisetum purpureum) using response surface methodology

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Clean Energy 2024年第5期8卷 241-251页

作者： Sunday C.Ikpeseni Samuel O.Sada Ufuoma j.Efetobor Henry O.Orugba Mathias Ekpu Hilary I.Owamah jeremiah l.Chukwuneke Solomon Oyebisi Uche P.Onochie Department of Mechanical Engineering Delta State UniversityP.M.B.1AbrakaNigeria Department of Mechanical Engineering Delta State PolytechnicP.M.B.3Otefe-OgharaNigeria Department of Chemical Engineering Delta State UniversityP.M.B.1AbrakaNigeria Department of Civil Engineering Delta State UniversityP.M.B.1AbrakaNigeria Department of Mechanical Engineering Nnamdi Azikiwe UniversityP.M.B.2025AwkaNigeria Department of Civil Engineering Covenant UniversityP.M.B.1023OttaNigeria Department of Civil Engineering The University of the South PacificP.M.B.9072SuvaFiji Department of Mechanical Engineering Delta State University of Science and TechnologyP.M.B.5OzoroNigeria

The need to increase bio-oil yield from biomass and enhance its fuel properties has driven research into optimizing the pyrolysis *** study investigated the influence of three key process parameters—temperature,heating rate,and nitrogen flow rate—on the pyrolysis of elephant grass(Pennisetum purpureum)in a fixed-bed *** surface methodology was used to study the im-pact of the aforementioned variables on bio-oil yield to improve its production *** analysis of the biomass revealed 79.24 wt%volatile matter,14.22 wt%fixed carbon,and 5.86%ash,with ultimate analysis showing 45.44%carbon,5.59%hydrogen,and 40.95%*** high volatile matter content and favourable carbon and hydrogen percentages indicate that elephant grass is a vi-able energy source due to its potential for high bio-oil yield and energy *** resulting bio-oil exhibited a higher heating value of 20.9 Mj/kg,indicating its suitability for various heating applications.A second-order regression model was developed for bio-oil yield,with optimal conditions identified as a temperature of 550°C,a heating rate of 17°C/min,and a nitrogen flow rate of 6 ml/*** study achieved an optimal bio-oil yield of 59.03 wt%,and the model’s high R²value of 0.8683 from analysis of variance analysis confirmed its predictive *** research highlights elephant grass as a sustainable feedstock for bio-oil production,offering valuable insights into optimizing pyrolysis conditions to enhance bio-oil yield,thus advancing biofuel technology.

关键词： renewable energy biomass sustainable feedstock regression model fixed-bed reactor

Carbon Monoxide as C1 Building Block in Fine Chemical Synthesis

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Chinese journal of Chemistry 2024年第2期42卷 199-221页

作者： Rui M.B.Carrilho Mario j.F.Calvete Gabor Mikle laszlo Kollar Mariette M.Pereira University of Coimbra Coimbra Chemistry CentreDepartment of ChemistryRua Larga3004-535 CoimbraPortugal Department of General and Inorganic Chemistry and ElKH-PTE Research Groupfor Selective Chemical Syntheses University of PecsIfjusag utja 6H-7624 PecsHungary

Carbon monoxide(CO)has become one of the most relevant and versatile renewable C1 building blocks for chemical synthesis,especially in the fine chemicals industry,due to the development of efficient and selective catalysts for its *** this review,we present a comprehensive critical analysis of the last 10 years literature on the use of CO as a renewable feedstock for fine chemicals *** review is organized by type of catalytic reaction,namely alkene and alkyne carbonylation,hydroformylation,carbonylation of aryl halides,carbonylative cross-coupling and C-H *** examples of the synthesis of relevant building blocks and/or known pharmaceuticals are *** is placed on examples of utilizing CO as the C1 building block in one or more catalytic *** catalyst used and the reaction conditions are consistently presented throughout all of the examples.

关键词： Carbon monoxide Fine chemicals Carbonylation Hydroformylation C-H functionalization

Exploring the associations between skeletal muscle echogenicity and physical function in aging adults:A systematic review with meta-analyses

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journal of Sport and Health Science 2024年第6期13卷 820-840页

作者： Dustin j.Oranchuk Stephan G.Bodkin Katie l.Boncella Michael O.Harris-love Muscle Morphology Mechanicsand Performance LaboratoryDepartment of Physical Medicine and RehabilitationUniversity of Colorado Anschutz Medical CampusAuroraCO 80045USA Department of Physical Therapy

Background:Assessment and quantification of skeletal muscle within the aging population is vital for diagnosis,treatment,and injury/disease *** clinical availability of assessing muscle quality through diagnostic ultrasound presents an opportunity to be utilized as a screening tool for function-limiting ***,relationships between muscle echogenicity and clinical functional assessments require authoritative ***,we aimed to(a) synthesize the literature to assess the relationships between skeletal muscle echogenicity and physical function in older adults(≥60 years),(b) perform pooled analyses of relationships between skeletal muscle echogenicity and physical function,and(c) perform sub-analyses to determine between-muscle ***:CINAHl,Embase,MEDlINE,PubMed,and Web of Science databases were systematically searched to identify articles relating skeletal muscle echogenicity to physical function in older ***-of-bias assessments were conducted along with funnel plot ***-analyses with and without sub-analyses for individual muscles were performed utilizing Fisher's Z transformation for the most common measures of physical ***'s Z was back-transformed to Pearson's r for ***:Fifty-one articles(n=5095,female=~2759,male=~2301,72.5± 5.8 years,mean±SD(1 study did not provide sex descriptors))were extracted for review,with previously unpublished data obtained from the authors of 13 *** rectus femoris(n=34) and isometric knee extension strength(n=22) were the most accessed muscle and physical qualities,*** relationship between quadriceps echogenicity and knee extensor strength was moderate(n=2924,r=-0.36(95% confidence interval:-0.38 to-0.32),p ll other meta-analyses(grip strength,walking speed,sit-to-stand,timed up-and-go) resulting in slightly weaker correlations(r:-0.34 to-0.23,all p lyses determined minimal differences in predicti

关键词： Correlations Echo intensity Elderly Intramuscular fat Strength

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On B(5,20)2-Groups

Algebra Colloquium 2024年第3期31卷 429-440页

作者： E.A.Alhassan Y.l.Tan Y.li School of Mathematical Sciences Jiangsu UniversityZhenjiangJiangsu 212013China Department of Mathematics Brock UniversitySt.CatharinesOntario L2S 3A1Canada

A finite group G is said to be a B(n,k)group if for any n-element subset[a_(1),…,a_(n)]of G,|{a_(i)a_(j)|1≤i,j≤n}|≤*** is of interest to characterize the structure of B(n,k)groups for n(n+1)/2≤k≤n^(2)-*** B(5,k)groups for 15≤k≤19 have been investigated by several *** this paper,we give a complete characterization of B(5,20)2-groups by showing there are five classes of such groups which are nontrivial and nonabelian.

关键词： B(n,k)groups small squaring property central extension GAP