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Reperfusion injury after critical intestinal ischemia and its correction with perfluorochemical emulsion "perftoran"

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World Journal of Gastroenterology 2005年第45期11卷 7084-7090页

作者： Vyacheslav Leontjevich Kozhura Dmitriy Alexeevich Basarab Marina Innokentievna Timkina Arkadiy Mikhailivich Golubev vasiliy Ivanovich Reshetnyak Viktor Vasiljevich Moroz Research Institute of General Reanimatology

AIM： To investigate the anti-ischemic properties of perfluorochemical emulsion ＂perftoran＂ in mesenteric region. METHODS： Experiments were conducted on 146 nonlinear white male rats weighing 200-350 g. Partial critical intestinal ischemia was induced by thorough atraumatic strangulation of 5-6 cm jejunal loop with its mesentery for 90 rain. Global critical intestinal ischemia was made by atraumatic occlusion of the cranial mesenteric artery （CMA） for 90 rain also. Perftoran （PF, 0.8-1.0 mL per 100 g） in experimental groups or 0.9% sodium chloride in control groups was injected at 75 rain of ischemic period. Mean systemic arterial blood pressure （BPM） registration, intravital microscopy and morphological examination of ischemic intestine and its mesentery were performed in both groups. RESULTS： During 90 min of reperfusion, BPM progressively decreased to 27.3±7.4% after PF administration vs 38.6±8.0% in the control group of rats with partial intestinal ischemia （NS） and to 50.3±6.9% vs 53.1±5.8% in rats after global ischemia （NS）. During the reperfusion period, full restoration of microcirculation was never registered; parts with restored blood flow had leukocyte and erythrocyte stasis and intra-vascular clotting, a typical ＂non-reflow＂phenomenon. The reduction of mesenteric 50-400 μm feeding artery diameter was significantly less in the PF group than in the control group （24±5.5% vs 45.2±3.6%, P〈0.05） 5 min after partial intestinal ischemia. This decrease progressed but differences between groups minimized at the 90th min of reperfusion （41.5±4.2% and 50.3±2.8%, respectively）. In reperfusion of rat＇s intestine, a significant mucosal alteration was registered. Villous height decreased 2.5-3 times and the quantity of crypts decreased more than twice. In the group of rats administered PF, intestinal mucosal layer was protected from irreversible post-ischemic derangement during reperfusion. Saved cryptal epithelial cells were the source of regeneration of the epithelium

关键词： Rats Ischemia-reperfusion injury Gut Intestine Perfluorocarbon emulsions

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CATALYTIC PROPERTIES OF NANOFIBROUS CARBON IN SELECTIVE OXIDATION OF HYDROGEN SULPHIDE

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China Particuology 2006年第2期4卷 70-72页

作者： Gennady G. Kuvshinov vasiliy V. Shinkarev Alexey M. Glushenkov Maxim N. Boyko Dmitriy G. Kuvshinov Department of Chemical Engineering Novosibirsk State Technical University Pr.Karla Marksa 20 Novosibirsk Russia Group of catalytic technologies of carbon materials synthesis Boreskov Institute of Catalysis Pr.Akademika Lavrentieva 5 Novosibirsk Russia

Nanofibrous carbonaceous materials （NFC） as a new class of materials having many applications, can catalyze the selective oxidation of H2S to sulfur. The correlation between NFC structure and its activity and selectivity in H2S oxidation was determined. It is demonstrated that selectivity can be improved if NFC with more ordered structure be synthesized and the portion of the original catalyst in carbon be reduced by increasing the carbon accumulated in the catalyst.

关键词： nanostructure carbon nanofiber carbon nanotubes carbon yield selective oxidation of the hydrogen sulfide oxygen excess

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数位板汽车侧视图二维渲染教程

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工业设计 2015年第2期 42-43页

作者： vasiliy Kanevskiy

作者vasiliy Kanevskiy与大家分享通过Wacom Intous4L手绘板在Photoshop CS5完成的概念设计草图二维渲染教程。1、在PS建立5000 x 2800 NX 300 DPI画布,开始设计创作,不要太在意线条,以正确透视为主。2、降低线稿的透明度,再在草图的基... 详细信息

作者vasiliy Kanevskiy与大家分享通过Wacom Intous4L手绘板在Photoshop CS5完成的概念设计草图二维渲染教程。1、在PS建立5000 x 2800 NX 300 DPI画布,开始设计创作,不要太在意线条,以正确透视为主。2、降低线稿的透明度,再在草图的基础上画出精准的干净线条。3、填充灰色,在图层样式中把混合模式改成正片叠加。

关键词：教程二维线条国画技法车身

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Electrodeposition of Palladium Coatings from Iminodiacetate Electrolyte

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American Journal of Analytical Chemistry 2013年第11期4卷 642-646页

作者： Valeriy S. Kublanovsky vasiliy N. Nikitenko Kostiantyn P. Rudenko V. I. Vernadskii Institute of General and Inorganic Chemistry NAS Ukraine Kyiv Ukraine

The ionic composition of an iminodiacetate electrolyte as a function of solution composition and pH has been deter-mined. The kinetic parameters (exchange currents and apparent transfer coefficients) of the electroreduction of a palla-dium(II) bis-iminodiacetate complex from an electrolyte containing excess ligand have been calculated. It has been shown that the rate of the electrode process is controlled by the diffusion of reduced ions to the electrode surface and by the electron-transfer reaction. The possibility of using iminodiacetate electrolyte for palladium plating for the deposition of fine-crystalline adherent and ductile palladium coatings has been examined.

关键词： Palladium Iminodiacetate Complex Compounds Electrodeposition Palladium Coatings

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Laser speckle contrast imaging compared with indocyanine green in renal perfusion of a porcine model

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Current Urology 2023年第2期17卷 141-145页

作者： Jonathan Noël Anya Mascarenhas Chibueze A.Nwaiwu Yao Liu Marcio Moschovas vasiliy E.Buharin John Oberlin Saloni Mehrotra Alyson F.Dechert Peter C.W.Kim Vipul Patel Department of Urology AdventHealth Global Robotics InstituteCelebrationFL 34747USA Harvard University CambridgeMAUSA Department of Surgery Warren Alpert Medical School of Brown UniversityRhode Island HospitalProvidenceRIUSA Department of Research Activ Surgical Inc.BostonMAUSA Department of Surgery University of BuffaloBuffaloNYUSA

Background:When viewed under near-infrared light,indocyanine green(ICG)signal for kidney perfusion can be utilized in partial *** speckle contrast imaging(LSCI)uses coherent light to detect perfusion during real-time laparoscopic *** and methods:Laser speckle contrast imaging or ActivSight,an imaging sensor adapter,was used during laparoscopy of an anesthetized porcine kidney ***'s"perfusion mode"and"quantification mode"displayed the blood flow as a heatmap and numerical signal intensity,***:After the upper segmental renal artery was clamped,ICG was seen in the lower pole,and LSCI showed low unit(dark color)quantification and perfusion in the upper *** green was retained in the lower pole after the upper segmental artery was unclamped,and LSCI perfusion was demonstrated in the entire ***:Laser speckle contrast imaging is a dye-free,repeatable,real-time adjunct for renal parenchymal perfusion assessment applicable to minimally invasive renal surgery to complement the technology of ICG near-infrared fluorescence and advance digital surgery.

关键词： Augmented reality Indocyanine green Laser speckle contrast imaging Perfusion Selective arterial clamping

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Expression of Acyl-lipid 12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato

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Journal of Integrative Plant Biology 2010年第3期52卷 289-297页

作者： Reza Maali Amiri Natalia O. Yur'eva Khristina R. Shimshilashvili Irina V. Goldenkova-Pavlova vasiliy P. Pchelkin Elmira I. Kuznitsova Vladimir D. Tsydendambaev Tamara I. Trunova Dmitry A. Los Gholamreza Salehi Jouzani Alexander M. Nosov Department of Agronomy and Plant Breeding University College of Agriculture and Natural Resources of the University of Tehran K.A.Timiryazev Institute of Plant Physiology Russian Academy of Sciences (RAS) Vavilov Institute of General Genetics Agricultural Biotechnology Researches Institute of Iran (ABRII) Department of Plant Physiology Moscow State University

We report the expression profile of acyl-lipid △12-desaturase （desA） gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic （Escherichia coil） and eukaryotic （Solanum tuberosum） cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase （licBM3） was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative （Petri dish test, electrophoregram and zymogram） and quantitative methods （spectrometry and gas liquid chromatography assays）. The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18：2 and 18：3 fatty acids in primary transgenic plants was observed for lines 2 （73%） and 3 （41%）. Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction.

关键词： Expression of Acyl-lipid 12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato gene

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CVD生长多壁碳纳米管的拉曼光谱径向呼吸振动模式观察(英文)

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新型炭材料 2015年第5期30卷 385-390页

作者： Valeriy V.Bolotov vasiliy E.Kan Egor V.Knyazev Peter M.Korusenko Sergey N.Nesov Yuriy A.Sten`kin Viktor A.Sachkov Irina V.Ponomareva Omsk Scientific Center of Siberian Branch Russian Academy of Sciences

采用拉曼光谱仪、透射电镜、俄歇能谱仪、X射线光电子能谱仪等研究在Si O2/Si基底上化学沉积的多壁碳纳米管(MWCNTs)经390℃的空气中热处理120 min前后与HCl溶液化学处理前后的结构变化情况。拉曼光谱集中测试低频(250-300 cm-1)带。结... 详细信息

采用拉曼光谱仪、透射电镜、俄歇能谱仪、X射线光电子能谱仪等研究在Si O2/Si基底上化学沉积的多壁碳纳米管(MWCNTs)经390℃的空气中热处理120 min前后与HCl溶液化学处理前后的结构变化情况。拉曼光谱集中测试低频(250-300 cm-1)带。结果表明,经热处理和化学处理后,在250-300 cm-1形成的拉曼带在峰位和半宽几乎没变。由透射电镜可知,小直径碳纳米管的内径值与拉曼光谱测试结果一致。这些结果表明,低频带产生于小直径碳纳米管的内壁中碳原子的径向呼吸振动。

关键词：碳纳米管径向振动模式拉曼化学气相沉积显微镜

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颜色的质感乌克兰蓝色办公室

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室内设计与装修 2011年第5期 88-91页

作者： Sergey Makhno Butenko vasiliy 时颜

办公空间,实用最重要,但也是接待客户、展示企业文化的重要部分。让客户进入办公室后就能感受到该企业的魅力,从而增加客户对该企业的好感度,显得极其重要。位于乌克兰首都基辅的蓝色办公室（Azure Office）,

关键词：办公室乌克兰蓝色质感颜色企业文化办公空间客户

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DNA damage and cell death assessment in patients with severe multiple trauma using comet assay

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Health 2010年第5期2卷 412-417页

作者： Aliy K. Zhanataev Victor V. Moroz Andrey D. Durnev Maria Yu. Muravyeva vasiliy I. Reshetnyak Science Research Institute of Pharmacology Russian Academy of Medical Sciences Science Research Institute of General Reanimatology Russian Academy of Medical Sciences

Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet assay was used to measure DNA strand breakage, 8-oxoguanine levels and apoptotic and necrotic nuclei in after admission (day 0) and on days 3, 5, 7 and 15. TAOS was determined by colorimetric method. Results: Trauma patients had high DNA damage at admission (p < 0.01), that further increased with maximum value on day 5 (p < 0.001). On day 15 the degree of DNA damage remained significantly elevated (p < 0.01). No significant difference in the 8-oxoguanine levels at all days examined was found. Patients had a high percentage of apoptotic and necrotic comets at admission, with maximum values on days 3 and 5. A significantly lower TAOS was observed in patients on admission and days 3, 5, 7 and 15 (p < 0.001 in all cases). A decreasing of TAOS on days 7 and 15 compared to that on admission (p < 0.05) was observed. Conclusions: Blood cells from severe trauma patients’ display increased DNA damage associated with apoptosis and necrosis. Reduced plasma TAOS and a tendency to increase of 8-oxoguanine in DNA was observed.

关键词： Apoptosis Necrosis Multiple Trauma DNA-Comet Assay 8-Oxoguanine Total Antioxidant Status

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4-甲基-1,2,3-噻二唑-5-甲酸芳酯的合成及生物活性

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农药学学报 2010年第3期12卷 255-263页

作者：王守信米娜范志金付一峰黄云王唤张正财张聚方宋海斌 BELSKAYA N.P. bakulev V.A. 农业部农药化学与应用重点开放实验室北京100193 南开大学元素有机化学国家重点实验室天津300071 四川农业大学植物保护学院四川雅安625014 The Ural Federal University Named After the First President of Russia B.N.Yeltsin Yeltsin UrFU

为了寻找高效广谱的植物激活剂候选化合物,以三乙胺为缚酸剂,通过4-甲基-1,2,3-噻二唑-5-甲酰氯与酚类化合物在二氯甲烷中于室温下反应,制得一系列共20个4-甲基-1,2,3-噻二唑-5-甲酸芳酯化合物,其中9个化合物未见文献报道,所有化合物的... 详细信息

为了寻找高效广谱的植物激活剂候选化合物,以三乙胺为缚酸剂,通过4-甲基-1,2,3-噻二唑-5-甲酰氯与酚类化合物在二氯甲烷中于室温下反应,制得一系列共20个4-甲基-1,2,3-噻二唑-5-甲酸芳酯化合物,其中9个化合物未见文献报道,所有化合物的结构均得到了核磁共振氢谱、红外光谱、高分辨质谱的表征和确认。选择化合物2g培养了单晶,利用X-射线单晶衍射确证了该类化合物的空间结构。杀菌活性测定结果表明:部分化合物在50μg/mL下具有显著的离体杀菌活性,其中化合物2c,2n和2o具有广谱的杀菌活性,其EC50值在3.46～23.30μg/mL之间。生物活性测定结果表明,部分化合物显示了较好的抗病毒活性,大部分化合物对烟草花叶病毒(TMV)具有较好的钝化效果,2a,2c,2d,2e,2f,2g,2l,2o具有较好的诱导烟草抗TMV的活性。

关键词： 1 2 3-噻二唑合成生物活性植物激活剂

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