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Protective effect of thodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury

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Neural Regeneration Research 2020年第4期15卷 690-696页

作者： Xiao-Qin ha Bo Yang Huai-Jing Hou Xiao-ling Cai Wan-Yuan Xiong Xu-Pan Wei lanzhou University Second Hospital LanzhouGansu ProvinceChina Department of Clinical laboratory Lanzhou General Hospital of Lanzhou Military Area CommandLanzhouGansu ProvinceChina School of Clinical Medicine Gansu University of Traditional Chinese MedicineLanzhouGansu ProvinceChina School of life Science and Engineering Lanzhou University of TechnologyLanzhouGansu ProvinceChina

Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue *** study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons *** establishing the model,the rats were randomly divided into five *** in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μl).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 *** in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with *** in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/ml in a 50-μl cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected *** in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with *** week after treatment,exercise recovery was evaluated with a modified combined behavioral score ***-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord *** of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal *** staining was applied to detect HIF-1 protein levels in the spinal *** results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(Plene blue staining

关键词： acute spinal cord injury adenovirus adenovirus gene IX bone marrow mesenchymal stem cells combined behavioral score scale HIF-1α nerve regeneration nerve repair Rhodiola rosea Sry

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Chinese Medical Journal 1997年第6期110卷 96-98页

作者： Raymondliang C.k.lee FrederickChen Y.l.kwong C.S.Chim W.Y.Au S.Y.ha C.F.Chan Department of Medicine University of Hong Kong Queen Mary Hospital Pokfulam Hong Kong.

ＵｎｒｅｌａｔｅｄｍａｒｏｗｄｏｎｏｒｒｅｇｉｓｔｒｙｆｏｒＣｈｉｎｅｓｅＲａｙｍｏｎｄＬｉａｎｇ，Ｃ．Ｋ．Ｌｅｅ，ＦｒｅｄｅｒｉｃｋＣｈｅｎ，Ｙ．Ｌ．Ｋｗｏｎｇ，Ｃ．Ｓ．Ｃｈｉｍ，Ｗ．Ｙ．Ａｕ，Ｓ．Ｙ．Ｈａ，Ｃ．Ｆ... 详细信息

ＵｎｒｅｌａｔｅｄｍａｒｏｗｄｏｎｏｒｒｅｇｉｓｔｒｙｆｏｒＣｈｉｎｅｓｅＲａｙｍｏｎｄＬｉａｎｇ，Ｃ．Ｋ．Ｌｅｅ，ＦｒｅｄｅｒｉｃｋＣｈｅｎ，Ｙ．Ｌ．Ｋｗｏｎｇ，Ｃ．Ｓ．Ｃｈｉｍ，Ｗ．Ｙ．Ａｕ，Ｓ．Ｙ．Ｈａ，Ｃ．Ｆ．Ｃｈａｎ，Ｙ．Ｌ．ＬａｕａｎｄＢ...

关键词： registry donor

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long-Term Conservation Agriculture and Intensified Cropping Systems:Effects on Growth,Yield,Water,and Energy-use Efficiency of Maize in Northwestern India

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Pedosphere 2018年第6期28卷 952-963页

作者： Chiter M.PARIhaR Malu R.YADAV Shankar l.JAT Aditya k.SINGH Bhupender kUMAR Vijay POONIYA Sanatan PRADhaN Rakesh k.VERMA Mangi l.JAT Raj k.JAT Muli D.PARIhaR hari S.NAYAk Yashpal S.SAhaRAWAT Indian Council of Agricultural Research-Indian Institute of Maize Research New Delhi 110012(India) Indian Council of Agricultural Research-Indian Agricultural Research Institute New Delhi 110012(India) International Maize and Wheat Improvement Center New Delhi 110012(India) Borlaug Institute for South Asia Samastipur 848125(India) Chaudhary Charan Singh haryana Agricultural University Hisar 125004(India) International Center for Agricultural Research in the Dry Areas Kabul 11082010(Afghanistan)

Conservation agriculture(CA)-based best-bet crop management practices may increase crop and water productivity, while conserving and sustaining natural resources. We evaluated the performance of rainy season maize during 2014 under an ongoing long-term trial(established in 2008) with three tillage practices, i.e., permanent bed(PB), zero tillage(ZT), and conventional tillage(CT) as main plots, and four intensified maize-based cropping systems, i.e., maize-wheat-mungbean, maize-chickpea-Sesbania(MCS), maizemustard-mungbean, and maize-maize-Sesbania) as subplot treatments. In the seventh rainy season of the experiment, maize growth parameters, yield attributes, yield, and water-and energy-use efficiency were highest at fixed plots under ZT. Maize growth parameters were significantly(P ld attributes, including cobs per m^2(7.8), cob length(0.183 m), grain rows per cob(13.8), and grains per row(35.6), were significantly higher under ZT than CT; however, no significant effect of cropping systems was found on maize growth and yield attributes. Zero tillage exhibited the highest maize productivity(4 589 kg ha^(-1)). However, among the cropping systems, MCS exhibited the highest maize productivity(4 582 kg ha^(-1)). In maize, water use was reduced by 80.2–120.9 mm ha-1under ZT and PB compared with CT, which ultimately enhanced the economic water-use efficiency by 42.0% and 36.6%, respectively. The ZT and PB showed a 3.5%–31.8% increase in soil organic carbon(SOC) at different soil depths(0–0.45 m), and a 32.3%–39.9% increase in energy productivity compared with CT. Overall, our results showed that CA-based ZT and PB practices coupled with diversified maize-based cropping systems effectively enhanced maize yield and SOC,as well as water-and energy-use efficiency, in northwestern India.

关键词： conventional tillage economic water-use efficiency permanent bed rainy season soil organic carbon zero tillage

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Association between serum cartilage oligomeric matrix protein and coronary artery calcification in maintenance hemodialysis patients

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Journal of Geriatric Cardiology 2020年第2期17卷 67-73页

作者： lahati ha Jun-Bao SHI hai-Yi YU kun YANG hai-Ning WANG Fang-Fang WANG Jiang-li haN Department of Cardiology and Institute of Vascular Medicine Peking University Third HospitalNHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesKey Laboratory of Molecular Cardiovascular ScienceMinistry of EducationBeijing Key Laboratory of Cardiovascular Receptors ResearchBeijingChina Department of Nephrology Peking University Third HospitalBeijingChina Department of Endocrinology Peking University Third HospitalBeijingChina

Background Coronary artery calcification(CAC)is common in end-stage renal disease(ESRD)patients,and the extent of CAC is closely related to cardiovascular outcomes in ESRD *** oligomeric matrix protein(COMP),as a component of the vascular matrix,has been found to be an inhibitor of arterial calcification in basic ***,there is no clinical research on the correlation between COMP and CAC in maintenance hemodialysis(MHD)*** aim of this study was to explore the relationship between serum COMP levels and CAC and cardiovascular events in MHD *** Serum COMP levels were compared between 54 MHD patients and 66 healthy *** patients were then divided into three groups according to the tertiles of the concentration of COMP level and were followed up for major adverse cardiac events(MACEs),which were defined as a combined end point of new onset angina pectoris,nonfatal myocardial infarction,heart failure,coronary artery revascularization,hospitalization due to angina pectoris and all-cause *** CAC score was calculated based on computed tomography *** The serum COMP level in MHD patients was significantly higher than that in the general population[984.23(248.43-1902.61)ng/ml vs.219.01(97.26-821.92)ng/ml,Plevels were positively correlated with CAC(r=0.313,P=0.021)and serum parathyroid hormone in MHD patients(r=0.359,Plinear regression suggested that after adjusting for age,fasting blood glucose(Glu)and glycosylated hemoglobin(HbAlc),CAC score was an independent predictor in the final model for COMP level(β=0.424,t=3.130,Pharacteristic(ROC)curve showed that COMP≥994 mg/ml had 68.0%sensitivity and 72.4%specificity for the prediction of severe CAC[area under the curve(AUC):0.674,P=0.030,95%CI:0.526-0.882].After a median follow-up of 16 months(8-24 months),there was no difference in the incidence rate of MACEs between the upper,middle and lower serum COMP gr

关键词： Biomarker Cartilage oligomeric matrix protein Coronary artery calcification Maintenance hemodialysis

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INVESTIGATION OF INFlUENCE OF HYDROGEN ATTACk ONFATIGUE PROPERTIES OF PRESSURE VESSEl STEElS

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Acta Metallurgica Sinica(English letters) 1998年第2期11卷 141-149页

作者： J.l.li k.F.Wang J.li Z.M.Yao W.ke Fushun Petroleum Inst Fushun China

Influence of hydrogen attack on mechanical and fatigue properties of 1020, 15CrMo and 2.25Cr-1Mo steels has been studied, which were exposed to gaseous hydrogen at pressure of 18MPa and 300-480℃ for up to 480h. The results of fatigue test showed that hydrogen attack (ha) resulted in an obvious change in fatigue properties of 1020 and 15CrMo steels, however, 2.25Cr-1Mo steel showed a lower susceptibility to hydrogen attack due to the addition of Cr and Mo. Fatigue crack behaviour for 1020 steel is attributed to hydrogen attack damage and the crack closure induced by the rough cracking surface, which lead to a valley on △kth～ha% plot. The change in the fatigue behaviour of 15CrMo steel is possibly caused by severe surface decarburization.

关键词： hydrogen attack pressure vessel steel threshold △k value crack closure

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Genetic reporter analysis reveals an expandable reservoir of OCT4+cells in adult skin

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Cell Regeneration 2014年第1期3卷 63-68页

作者： Anne limbourg Sabine Schnabel Vladimir J lozanovski l Christian Napp Teng-Cheong ha Tobias Maetzig Johann Bauersachs hassan Y Naim Axel Schambach Florian P limbourg Research Group Regenerative Agents HannoverGermany REBIRTH Cluster of Excellence HannoverGermany Integrated Research Center Transplantation(IFB-Tx) HannoverGermany Current affiliation:Department of Plastic Hand and Reconstructive SurgeryHannoverGermany Department of Cardiology and Angiology HannoverGermany Institute of Experimental Hematology OE6960 Hannover Medical SchoolCarl-Neuberg-Str.1D-30625 HannoverGermany Department of Physiological Chemistry Hannover Veterinary SchoolHannoverGermany Division of Hematology/Oncology Boston Children’s HospitalHarvard Medical SchoolHarvardUSA Department of Nephrology and Hypertension Hannover Medical SchoolHannoverGermany Department of General and Transplant Surgery University Hospital HeidelbergHeidelbergGermany Vascular Medicine and Transplantation Research Dept.of Nephrology and HypertensionOE 6841Hannover Medical SchoolCarl-Neuberg-Str.1D-30625 HannoverGermany

The transcription factor Oct4(Pou5f1)is a critical regulator of pluripotency in embryonic and induced pluripotent stem ***,Oct4 expression might identify somatic stem cell populations with inherent multipotent potential or a propensity for facilitated ***,analysis of Oct4 expression is confounded by Oct4 pseudogenes or non-pluripotency-related *** analysis of a transgenic Oct4-EGFP reporter mouse identified testis and skin as two principle sources of Oct4^(+)cells in postnatal *** the prevalence of GFP^(+)cells in testis rapidly declined with age,the skin-resident GFP^(+)population expanded in a cyclical *** cells were identified as epidermal stem cells dwelling in the stem cell niche of the hair follicle,which endogenously expressed all principle reprogramming factors at low ***,skin wounding or non-traumatic hair removal robustly expanded the GFP^(+)epidermal cell pool not only locally,but also in uninjured skin areas,demonstrating the existence of a systemic ***,the epithelial stem cell niche of the hair follicle harbors an expandable pool of Oct4^(+)stem cells,which might be useful for therapeutic cell transfer or facilitated reprogramming.

关键词： Oct4 Epidermal stem cells Skin Transgenic reporter mice

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New Aliphatic Alcohol and Ester Constituents from Rice Hulls of Oryza sativa

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Chinese Journal of Chemistry 2007年第6期25卷 843-848页

作者： CHUNG, Ill-Min AlI, Mohd CHUN, Se-Chul JIN, Cheng-Wu CHO, Dong-ha HONG, Seung-Beom AHMAD, Ateeque epartment of Applied life Science Konkuk University Seoul 143-701 South Korea Faculty of Pharmacy Hamdard University New Delhi 110 062 India kangwon National University School of Bioscience & Biotechnology Chunchon 200-701 South Korea

Six new aliphatic alcohol and ester compounds of n-octacos-9-enyl propionate （1）, 4-（3-formylheptyl）cyclohexyl 4-（cyclohexylcarbonyloxymethyl）cyclohexyl-carboxylate （2）, n-hentriacont-25-en-5α-ol （3）, n-hexetriacont-9,26-dien-8α,11β,23α-triol （4）, n-tetracontan-15α-ol （5） and n-tritetracontan-5α-ol （6） were isolated from the hulls of Oryza sativa. The structures were established on the basis of spectroscopic analysis.

关键词： Oryza sativa l. poaceaee rice hull compound aliphatic alcohol and ester constituents

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Porous Carbon Architecture Assembled by Cross-linked Carbon leaves with Implanted Atomic Cobalt for High-Performance li-S Batteries

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Nano-Micro letters 2021年第10期13卷 98-112页

作者： Ruirui Wang Renbing Wu Chaofan Ding Ziliang Chen Hongbin Xu Yongfeng liu Jichao Zhang Yuan ha Ben Fei Hongge Pan Department of Materials Science Fudan UniversityShanghai 200433People’s Republic of China State key laboratory of Silicon Materials and School of Materials Science and Engineering Zhejiang UniversityHangzhou 310027People’s Republic of China Shanghai Synchrotron Radiation Facility Zhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204People’s Republic of China Institute of Science and Technology for New Energy Xi’an Technological UniversityXi’an 710021People’s Republic of China

The practical application of lithium-sulfur batteries is severely hampered by the poor conductivity,polysulfide shuttle effect and sluggish reaction kinetics of sulfur ***,a hierarchi-cally porous three-dimension(3D)carbon architecture assembled by cross-linked carbon leaves with implanted atomic Co-N4 has been deli-cately developed as an advanced sulfur host through a SiO_(2)-mediated zeolitic imidazolate framework-l(ZIF-l)*** unique 3D architectures not only provide a highly conductive network for fast electron transfer and buffer the volume change upon lithiation-delithi-ation process but also endow rich interface with full exposure of Co-N4 active sites to boost the lithium polysulfides adsorption and *** to the accelerated kinetics and suppressed shuttle effect,the as-prepared sulfur cathode exhibits a superior electrochemical perfor-mance with a high reversible specific capacity of 695 mAh g^(−1) at 5 C and a low capacity fading rate of 0.053%per cycle over 500 cycles at 1 *** work may provide a promising solution for the design of an advanced sulfur-based cathode toward high-performance li-S batteries.

关键词： Single-atom Co 3D porous carbon architecture Cathode lithium–sulfur battery

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乙型肝炎病毒基因的整合机制及对宿主的影响

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世界华人消化杂志 2006年第8期14卷 743-746页

作者：哈明昊魏来北京大学肝病研究所北京大学人民医院北京市100044

乙型肝炎病毒是导致慢性肝炎、肝纤维化、肝细胞癌的主要病因,目前认为乙型肝炎病毒基因整合在其致病机制中具有重要作用,本文试就该领域内目前研究成果做一综述．

关键词：乙型肝炎病毒基因整合肝癌

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Effect of abiotic factors on understory community structures in moist deciduous forests of northern India

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Forestry Studies in China 2013年第4期15卷 261-273页

作者： Ashish k.MISHRA Soumit k.BEHERA kripal SINGH R.M.MISHRA l.B.ChaUDhaRY Bajrang SINGH Plant Diversity Systematics and Herbarium Division CSIR-National Botanical Research Institute Department of Environmental Biology Awadesh Pratap Singh University Restoration Ecology Group CSIR-National Botanical Research Institute

Understory vegetation controls, in a significant way, the regeneration of overstory trees, carbon sequestration and nutrient retention in tropical forests. Development and organization of understory vegetation depend on climate, edaphic and biotic factors which are not well correlated with plant community structures. This study aimed to explore the relationships between understory vegetation and abiotic factors in natural and planted forest ecosystems. A non-metric multidimensional scaling(NMS) ordination technique was applied to represent forest understory vegetation among five forest communities, i.e., a dry miscellaneous forest(DMF), a sal mixed forest(SMF), a teak plantation(TP), a low-land miscellaneous forest(lMF) and a savanna area(SAV) of the katerniaghat Wildlife Sanctuary, located in northern India. Microclimatic variables, such as photosynthetically active radiation(PAR), air temperature(AT), soil temperature(ST), ambient atmospheric CO 2 concentration, absolute air humidity(AH), physical and chemical soil properties as well as biological properties were measured. Understory species were assessed via 100 random quadrats(5 m × 5 m) in each of the five forests in which a total of 75 species were recorded encompassing 67 genera from 37 families, consisting of 32 shrubs and 43 plant saplings. DMF was the most dense forest with 34,068 understory individuals per ha of different species, whereas the lowest understory population(13,900 per ha) was observed in the savanna. Ordination and correlation revealed that microclimate factors are most important in their effect compared to edaphic factors, on the development of understory vegetation in the various forest communities in the north of India.

关键词： vegetation structure microclimate ordination phytosociotogy understory vegetation

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