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Applied Mathematics 2011年第2期2卷 254-257页

作者： Sunil Kumar Om P. Singh Sandeep dixit 不详

Many problems in physics like reconstruction of the radially distributed emissivity from the line-of-sight projected intensity, the 3-D image reconstruction from cone beam projections in computerized tomography, etc. lead naturally, in the case of radial symmetry, to the study of Abel’s type integral equation. Obtaining the physically relevant quantity from the measured one requires, therefore the inversion of the Abel’s integral equation. The aim of this letter is to present a user friendly algorithm to invert generalized Abel integral equation by using homotopy perturbation method. The stability of the algorithm is analysed. The validity and applicability of this powerful technique is illustrated through various particular cases which demonstrate its efficiency and simplicity in solving these types of integral equations.

关键词： Generalized Abel Integral Equation Homotopy Perturbation Method Noise Term Stability

Culture Medium Optimization for Enhanced Puerarin Production by Shake Flask Cultures of Pueraria tuberosa(***.) DC

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Culture Medium Optimization for Enhanced Puerarin Production...

Fine-scale evolutionary genetic insights into Anopheles gambiae X-chromosome

第二届国际整合植物生物学学术研讨会

作者： Vijai Singh Karwasara Vinod Kumar dixit Department of Pharmaceutical Sciences Dr.H.S.Gour VishwavidyalayaSagarMadhya Pradesh 470003India

Pueraria tuberosa DC(Fabaceae) is a perennial woody lianas which is highly valued in Ayurveda and traditional Chinese systems of *** major constituents of Pueraria tuberosa were isoflavonoids,mainly puerarin,an isoflavonoid C-glycoside found in the genus *** exhibits a great variety of biological and pharmacological *** is used clinically for treatment of cardiac and cerebral blood vascular *** has been shown to possess many pharmacological activities such as antioxidant activity,anti-inflammatory activities,and protective activity against ischemic *** recent investigations reported puerarin could induce apoptosis in cancer *** cell cultures remain a favoured methodology for biotechnological production of nature-based compounds despite large-scale destruction of natural habitat due to higher demand and overexploitation of ***,the conventional propagation of Pueraria is too *** is disparity in puerarin content due to varying seasonal and agro-ecological ***,our aim is to establish the in-vitro culture of Pueraria,and to optimize the effect of essential macro and micro-nutrient components and sucrose feeding on P. tuberosa cell growth and isoflavonoid *** the initiation of *** culture leaves, stems and petioles were used with different growth medium and varying concentrations of different plant growth *** media supplemented with 4.52 uM 2,4-D + 5.37 uM NAA + 4.65μM Kn showed the highest callogenesis *** month old friable callus cultures were used to initiate suspension *** estimation of puerarin was done by HPLC. The effects of carbon,nitrogen,phosphate,and copper on cell growth and production of the isoflavone puerarin by suspension cultures of Pueraria tuberosa was *** feeding strategy remarkably stimulated cell biomass growth and puerarin *** was also noticed that NH or NO as the solitary nitr

关键词： cell suspension culture macro-nutrient optimization Pueraria tuberosa puerarin

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Journal of Biomedical Science and Engineering 2009年第5期2卷 304-311页

作者： Hemlata Srivastava Jyotsana dixit Aditya P. Dash Aparup Das Evolutionary Genomics and Bioinformatics Laboratory National Institute of Malaria Research Sector 8 Dwarka New Delhi-110 077 India Present address: World Health Organization Southeast Asian Regional Office New Delhi India

Understanding the genetic architecture of indi-vidual taxa of medical importance is the first step for designing disease preventive strategies. To understand the genetic details and evolu-tionary perspective of the model malaria vector, Anopheles gambiae and to use the information in other species of local importance, we scanned the published X-chromosome se-quence for detail characterization and obtain evolutionary status of different genes. The te-locentric X-chromosome contains 106 genes of known functions and 982 novel genes. Majori-ties of both the known and novel genes are with introns. The known genes are strictly biased towards less number of introns;about half of the total known genes have only one or two in-trons. The extreme sized (either long or short) genes were found to be most prevalent (58% short and 23% large). Statistically significant positive correlations between gene length and intron length as well as with intron number and intron length were obtained signifying the role of introns in contributing to the overall size of the known genes of X-chromosome in An. gam-biae. We compared each individual gene of An. gambiae with 33 other taxa having whole ge-nome sequence information. In general, the mosquito Aedes aegypti was found to be ge-netically closest and the yeast Saccharomyces cerevisiae as most distant taxa to An. gambiae. Further, only about a quarter of the known genes of X-chromosome were unique to An. gambiae and majorities have orthologs in dif-ferent taxa. A phylogenetic tree was constructed based on a single gene found to be highly orthologous across all the 34 taxa. Evolutionary relationships among 13 different taxa were in-ferred which corroborate the previous and pre-sent findings on genetic relationships across various taxa.

关键词： Anopheles gambiae Comparative Genomics Evolution Malaria Orthologous Genes X-chromosome

Seasonal Variation of the Physicochemical Properties of Water Samples in Mahanadi Estuary, East Coast of India

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Journal of Environmental Protection 2013年第8期4卷 843-848页

作者： Pravat Ranjan dixit Biswabandita Kar Partha Chattopadhyay Chitta Ranjan Panda CSIR-Institute of Minerals and Materials Technology Bhubaneswar India KIIT University Bhubaneswar KIIT University Bhubaneswar India

The two major sources which are contributing to marine pollution are natural processes as well as anthropogenic activities. The natural process includes precipitation, erosion, weathering of crystal material whereas anthropogenic activities are urbanization, industrialization, mining and agricultural activities, etc. Mahanadi is the biggest river source of Odisha which joins the Bay of Bengal at Paradip. Paradip Township is an urbanized well-developed industrial township where various anthropogenic activities are contributing pollution to the water sources. In the present study, an attempt has been made to estimate and monitor the seasonal and spatial variation of physiochemical properties of the Mahanadi estuary, the East Coast coastal belt of India. The result revealed that there is a remarkable variation in the physiochemical parameters such as pH, salinity, TSS, DO, BOD, NO2N, NO3N, NH4N, TN, TP, SIO4 and Chl-a which are attributed to the runoff water getting discharged to the coastal water sources. It is being recommended to treat waste water and materials before discharging them to the marine water medium.

关键词： Physicochemical Water Pollution Mahanadi Estuary

The Role of Prophylactic Antibiotics for Percutaneous Procedures in Orthopaedic Surgery

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Surgical Science 2011年第6期2卷 348-352页

作者： A. Gulati A. dixit D. M. Williamson Great Western Hospital Swindon and Marlborough NHS Trust UK

Introduction This study investigates the current practice of surgeons in the United Kingdom with regards to their usage of prophylactic antibiotics for percutaneous orthopaedic procedures. Methods An electronic survey of 10 questions was devised and sent to all members of the British Orthopaedic Association. Three hundred and three replies were obtained (172 consultants, 131 trainees). Results Only half the numbers of orthopaedic surgeons would routinely use antibiotics for percutaneous K-wire fixation. Of the other half, 28% would never prescribe antibiotics and 22% would use them in special circumstances only. These ‘special circumstances’ were also not standardised. 92% of those who did prescribe antibiotics would administer single dose only and the majority (90%) would administer them during induction. There was no significant difference between trainees and consultants or between different orthopaedic procedures with regards to whether prophylactic antibiotics were prescribed or not. Discussion This survey highlights the split of opinion amongst practising orthopaedic surgeons as to the necessity or otherwise of antibiotic prophylaxis in percutaneous orthopaedic procedures. There are no reliable guidelines and further work should be carried out to investigate this subject.

关键词： Prophylactic Antibiotics Percutaneous Procedures K-Wires

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法律缺失与经济学

Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i>and Its Immunogenic Potential

作者： (dixit Avinash K.)迪克西特

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Advances in Bioscience and Biotechnology 2015年第7期6卷 443-451页

作者： Sunita Kumari Yadav Carmelita N. Marbaniang Vibhuti Sharma Aparna dixit School of Biotechnology Jawaharlal Nehru University New Delhi India RNA Biology Group Institute for Molecular Infection Biology University of Würzburg Würzburg Germany

Aeromonas hydrophila, a gram negative bacterium is a major fish pathogen and causes major economic losses to aquaculture industry. Outer membrane proteins play a significant role in its survival during different environmental conditions and bacterial pathogenesis. The outer membrane protein R (OmpR) is a member of the two-component regulatory system of Aeromonas hydrophila which differentially regulates the expression of OmpF or OmpC depending on the osmolarity conditions. Role of OmpR has been demonstrated in its virulence in other infectious bacteria and it is found to be a potential drug target/vaccine candidate. However, the OmpR of A. hydrophila has not been characterized. In the present study, we report recombinant expression, purification of the OmpR of A. hydrophila strain Ah17 in salt inducible E. coli GJ1158 cells. Leaky expression of rOmpR was confirmed by Western blot analysis using anti-6 × His antibody. The histidine tagged recombinant OmpR (rOmpR) (~29 kDa) was purified using Ni-NTA affinity chromatography from the soluble fraction of induced E. coli cells. The rOmpR was found to be highly immunogenic with end point titres of greater than 1:80,000. The anti-rOmpR antisera were capable of agglutinating live A. hydrophila cells, thus showing vaccine potential of the rOmpR.

关键词： OmpR Aeromonas Cross-Reactivity Outer Membrane Protein Expression E. coli GJ1158

Gall bladder carcinoma: Aggressive malignancy with protean loco-regional and distant spread

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World Journal of Clinical Cases 2015年第3期3卷 231-244页

作者： Amit Nandan Dhar Dwivedi Shivi Jain Ruhi dixit Department of Radiodiagnosis and Imaging Institute of Medical Sciences Banaras Hindu University Department of General Surgery Institute of Medical Sciences Banaras Hindu University

The most common malignancy of biliary tract is gallbladder cancer(GBC) which is the third most common cancer in gastrointestinal tract. It is a lethal disease for most patients in spite of growing awareness and improved diagnostic techniques. GBC has a verypoor prognosis and the 5 year survival rate is < 10%. Although etiology of the carcinoma of the gallbladder is still obscure, various factors have been implicated, cholelithiasis being the most frequent. The incidence of GBC worldwide is based on the gender, geography and ethnicity which suggest that both genetic and environmental factors can cause GBC. The major route of spread of gallbladder cancer(GC) is locoregional rather than distant. It spreads by lymphatic, vascular, neural, intraperitoneal, and intraductal routes. Sonography is usually the most common imaging test to evaluate symptoms of biliary tract disease including suspected GC. With recent advances in imaging modalities like multi-detector computed tomography(CT) scanners, magnetic resonance imaging-positron emission tomography/CT diagnosis of gallbladder cancer has improved. Studies have also targeted molecular and genetic pathways. Treatment options have included extended and radical surgeries and adjuvant chemotherapy. This review article deals in detail with important aspects of carcinoma gallbladder and its manifestations and challenges. Role of various imaging modalities in characterization and accurate staging has been discussed. The loco-regional spread of this aggressive malignancy is dealt explicitly.

关键词： Gallbladder cancer Loco-regional and distant spread Cholelithiasis Imaging Adenocarcinoma

Solution of the Generalized Abel Integral Equation by Using Almost Bernstein Operational Matrix

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American Journal of Computational Mathematics 2011年第4期1卷 226-234页

作者： Sandeep dixit Rajesh K. Pandey Sunil Kumar Om P. Singh 不详

A direct almost Bernstein operational matrix of integration is used to propose a stable algorithm for numerical inversion of the generalized Abel integral equation. The applicability of the earlier proposed methods was restricted to the numerical inversion of a part of the generalized Abel integral equation. The method is quite accurate and stable as illustrated by applying it to intensity data with and without random noise to invert and compare it with the known analytical inverse. Thus it is a good method for applying to experimental intensities distorted by noise.

关键词： Abel Inversion Bernstein Polynomials Almost Bernstein Operational Matrix Of Integration Noise Resistance