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A Compar.tive Study of ESTs Induced under.Dr.ught and Salinity Str.ss in Hyacinth Bean (<i>Lablab pur.ur.us</i>)

Amer.can Jour.al of Plant Sciences 2021年第5期12卷 840-857页

作者： S. Kokila varadahally r. devaraj Depar.ment of Biochemistr. Central college campus Bengaluru City University Bengaluru India

Abiotic str.ssor. like dr.ught and salinity ar. major.causes for.loss of agr.cultur.l pr.ductivity. Compar.son of expr.ssed sesquence tags (ESTs) under.differ.nt abiotic str.sses pr.vides insight into under.ying mecha... 详细信息

Abiotic str.ssor. like dr.ught and salinity ar. major.causes for.loss of agr.cultur.l pr.ductivity. Compar.son of expr.ssed sesquence tags (ESTs) under different abiotic stresses provides insight into underlying mechanism of stress response, and candidate genes to improve tolerance to abiotic and biotic stresses via breading and transgenic methods. In order to identify and compare stress-specific ESTs from drought and salinity stressed Hyacinth Bean, ten days old seedlings were subjected to respective stresses and RNA was extracted from control and stressed leaves for EST identification. 31 and 12 ESTs, respectively, were characterized from leaves of drought and salinity stressed seedlings of Hyacinth Bean, Lablab purpureusr.ana;"> by differ.ntial display r.-PCr.using identical combinations of 48 pr.mer. and validated using quantitative r.-PCr. r.lative fold expr.ssion was higher.under.salt str.ss than dr.ught str.ss. Wher.as 19 EST over.xpr.ssed under.dr.ught, 12 EST wer. down r.gulated. Of the 12 EST under.salinity, 9 EST wer. downr.gulated and 3 EST upr.gulated. Putative functions pr.dicted fr.m sequence homology indicated that 11 dr.ught specific EST cor.esponded to metabolic functions, and 4 of them cor.esponded to tr.nscr.ption r.gulation. Under.salinity, 4 and 2 EST, r.spectively, cor.esponded to metabolic and r.A associated functions. Under.both str.sses, ther. wer. ESTs associated with unknown functions, whose char.cter.zation may thr.w light on the r.gulator. mechanism. Differ.ng number.of ESTs differ.ntially expr.ssed under.dr.ught and salt str.ss, and their.pr.dicted functionalities, suggested distinct set of r.sponse genes involved under.these two str.sses, despite a good number.of physiological player. being common. Fr.m the pr.dicted functions of ESTs, the pap

关键词： Hyacinth Bean Dr.ught Salinity DDr.-PCr ESTs

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A Compar.tive Study of ESTs Induced under.Dr.ught and Salinity Str.ss in Hyacinth Bean(Lablab pur.ur.us)

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Amer.can Jour.al of Plant Sciences 2021年第5期

作者： S. Kokila varadahally r. devaraj

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